Structure and expression of gene coding for a pupal cuticle protein of Bombyx mori
A specific protein termed as PCP accumulates in the newly synthesized pupal cuticle of the silkworm, Bombyx mori. We have cloned the genomic sequence encoding PCP and analyzed its structure. The PCP gene comprises two exons interspersed by a single intron approx. 5.8 kb in length. Transcription init...
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| Veröffentlicht in: | Biochimica et biophysica acta 1992-09, Vol.1132 (2), p.161-167 |
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| container_title | Biochimica et biophysica acta |
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| creator | Nakato, Hiroshi Izumi, Susumu Tomino, Shiro |
| description | A specific protein termed as PCP accumulates in the newly synthesized pupal cuticle of the silkworm,
Bombyx mori. We have cloned the genomic sequence encoding PCP and analyzed its structure. The PCP gene comprises two exons interspersed by a single intron approx. 5.8 kb in length. Transcription initiation sites of the PCP gene were located at nucleotide level. The 5′ flanking region of the gene contains a sequence homologous to the Pit-1 DNA recognition element of the rat prolactin and growth hormone genes. The developmental profile of the PCP precursor RNA in epidermal cells showed that the biosynthesis of PCP is regulated at the transcriptional level in a stage- and tissue-specific fashion during post-embryonic development. Administration of 20-hydroxyecdysone to the isolated abdomens prepared from the early fifth instar larvae provoked the accumulation of PCP mRNA in epidermis, suggesting that the molting hormone triggers the expression of PCP gene. |
| doi_str_mv | 10.1016/0167-4781(92)90007-M |
| format | Article |
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Bombyx mori. We have cloned the genomic sequence encoding PCP and analyzed its structure. The PCP gene comprises two exons interspersed by a single intron approx. 5.8 kb in length. Transcription initiation sites of the PCP gene were located at nucleotide level. The 5′ flanking region of the gene contains a sequence homologous to the Pit-1 DNA recognition element of the rat prolactin and growth hormone genes. The developmental profile of the PCP precursor RNA in epidermal cells showed that the biosynthesis of PCP is regulated at the transcriptional level in a stage- and tissue-specific fashion during post-embryonic development. Administration of 20-hydroxyecdysone to the isolated abdomens prepared from the early fifth instar larvae provoked the accumulation of PCP mRNA in epidermis, suggesting that the molting hormone triggers the expression of PCP gene.</description><identifier>ISSN: 0167-4781</identifier><identifier>ISSN: 0006-3002</identifier><identifier>EISSN: 1879-2634</identifier><identifier>DOI: 10.1016/0167-4781(92)90007-M</identifier><identifier>PMID: 1390888</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Amino Acid Sequence ; Animals ; B. mori ; Base Sequence ; Biological and medical sciences ; Bombyx - genetics ; Bombyx - growth & development ; Bombyx mori ; Cloning, Molecular ; Cuticle protein ; DNA ; Ecdysone - physiology ; Fundamental and applied biological sciences. Psychology ; Gene cloning ; Gene Expression Regulation ; Genes. Genome ; Genomic sequence ; Insect Proteins ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; Molting hormone ; Protein Biosynthesis ; Proteins - genetics ; Restriction Mapping ; Transcription, Genetic ; Transcriptional regulation</subject><ispartof>Biochimica et biophysica acta, 1992-09, Vol.1132 (2), p.161-167</ispartof><rights>1992</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c483t-e2ff9cf6fbe5e57e2cf4b9347124c7fbfee51af32f45b061ed4a68902bbdf8923</citedby><cites>FETCH-LOGICAL-c483t-e2ff9cf6fbe5e57e2cf4b9347124c7fbfee51af32f45b061ed4a68902bbdf8923</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5611330$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1390888$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nakato, Hiroshi</creatorcontrib><creatorcontrib>Izumi, Susumu</creatorcontrib><creatorcontrib>Tomino, Shiro</creatorcontrib><title>Structure and expression of gene coding for a pupal cuticle protein of Bombyx mori</title><title>Biochimica et biophysica acta</title><addtitle>Biochim Biophys Acta</addtitle><description>A specific protein termed as PCP accumulates in the newly synthesized pupal cuticle of the silkworm,
Bombyx mori. We have cloned the genomic sequence encoding PCP and analyzed its structure. The PCP gene comprises two exons interspersed by a single intron approx. 5.8 kb in length. Transcription initiation sites of the PCP gene were located at nucleotide level. The 5′ flanking region of the gene contains a sequence homologous to the Pit-1 DNA recognition element of the rat prolactin and growth hormone genes. The developmental profile of the PCP precursor RNA in epidermal cells showed that the biosynthesis of PCP is regulated at the transcriptional level in a stage- and tissue-specific fashion during post-embryonic development. Administration of 20-hydroxyecdysone to the isolated abdomens prepared from the early fifth instar larvae provoked the accumulation of PCP mRNA in epidermis, suggesting that the molting hormone triggers the expression of PCP gene.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>B. mori</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Bombyx - genetics</subject><subject>Bombyx - growth & development</subject><subject>Bombyx mori</subject><subject>Cloning, Molecular</subject><subject>Cuticle protein</subject><subject>DNA</subject><subject>Ecdysone - physiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene cloning</subject><subject>Gene Expression Regulation</subject><subject>Genes. Genome</subject><subject>Genomic sequence</subject><subject>Insect Proteins</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Molting hormone</subject><subject>Protein Biosynthesis</subject><subject>Proteins - genetics</subject><subject>Restriction Mapping</subject><subject>Transcription, Genetic</subject><subject>Transcriptional regulation</subject><issn>0167-4781</issn><issn>0006-3002</issn><issn>1879-2634</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1r1UAUhgex1Gv1HyjMQkQXqfOV-dgU9KJtoaXgx3qYTM6UkSQTZxK5_fcmvZd2VxeHszjPeXl5EHpDySklVH5aRlVCafrBsI-GEKKq62doQ7UyFZNcPEebB-QFelnK74UhkspjdEy5IVrrDfr-Y8qzn-YM2A0tht2YoZSYBpwCvoUBsE9tHG5xSBk7PM6j67Cfp-g7wGNOE8R79Evqm7sd7lOOr9BRcF2B14d9gn59-_pze1Fd3Zxfbj9fVV5oPlXAQjA-yNBADbUC5oNoDBeKMuFVaAJATV3gLIi6WWpDK5zUhrCmaYM2jJ-g9_vcpcafGcpk-1g8dJ0bIM3FKk6NYUT9F6RSK8nomij2oM-plAzBjjn2Lt9ZSuzq3K5C7SrUGmbvndvr5e3tIX9uemgfn_aSl_u7w90V77qQ3eBjecBqSSnnZMHO9hgs0v5GyLb4CIOHNmbwk21TfLrHP8qLngM</recordid><startdate>19920924</startdate><enddate>19920924</enddate><creator>Nakato, Hiroshi</creator><creator>Izumi, Susumu</creator><creator>Tomino, Shiro</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19920924</creationdate><title>Structure and expression of gene coding for a pupal cuticle protein of Bombyx mori</title><author>Nakato, Hiroshi ; Izumi, Susumu ; Tomino, Shiro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c483t-e2ff9cf6fbe5e57e2cf4b9347124c7fbfee51af32f45b061ed4a68902bbdf8923</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>B. mori</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Bombyx - genetics</topic><topic>Bombyx - growth & development</topic><topic>Bombyx mori</topic><topic>Cloning, Molecular</topic><topic>Cuticle protein</topic><topic>DNA</topic><topic>Ecdysone - physiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene cloning</topic><topic>Gene Expression Regulation</topic><topic>Genes. Genome</topic><topic>Genomic sequence</topic><topic>Insect Proteins</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Molting hormone</topic><topic>Protein Biosynthesis</topic><topic>Proteins - genetics</topic><topic>Restriction Mapping</topic><topic>Transcription, Genetic</topic><topic>Transcriptional regulation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nakato, Hiroshi</creatorcontrib><creatorcontrib>Izumi, Susumu</creatorcontrib><creatorcontrib>Tomino, Shiro</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biochimica et biophysica acta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nakato, Hiroshi</au><au>Izumi, Susumu</au><au>Tomino, Shiro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Structure and expression of gene coding for a pupal cuticle protein of Bombyx mori</atitle><jtitle>Biochimica et biophysica acta</jtitle><addtitle>Biochim Biophys Acta</addtitle><date>1992-09-24</date><risdate>1992</risdate><volume>1132</volume><issue>2</issue><spage>161</spage><epage>167</epage><pages>161-167</pages><issn>0167-4781</issn><issn>0006-3002</issn><eissn>1879-2634</eissn><abstract>A specific protein termed as PCP accumulates in the newly synthesized pupal cuticle of the silkworm,
Bombyx mori. We have cloned the genomic sequence encoding PCP and analyzed its structure. The PCP gene comprises two exons interspersed by a single intron approx. 5.8 kb in length. Transcription initiation sites of the PCP gene were located at nucleotide level. The 5′ flanking region of the gene contains a sequence homologous to the Pit-1 DNA recognition element of the rat prolactin and growth hormone genes. The developmental profile of the PCP precursor RNA in epidermal cells showed that the biosynthesis of PCP is regulated at the transcriptional level in a stage- and tissue-specific fashion during post-embryonic development. Administration of 20-hydroxyecdysone to the isolated abdomens prepared from the early fifth instar larvae provoked the accumulation of PCP mRNA in epidermis, suggesting that the molting hormone triggers the expression of PCP gene.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>1390888</pmid><doi>10.1016/0167-4781(92)90007-M</doi><tpages>7</tpages></addata></record> |
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| ispartof | Biochimica et biophysica acta, 1992-09, Vol.1132 (2), p.161-167 |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Amino Acid Sequence Animals B. mori Base Sequence Biological and medical sciences Bombyx - genetics Bombyx - growth & development Bombyx mori Cloning, Molecular Cuticle protein DNA Ecdysone - physiology Fundamental and applied biological sciences. Psychology Gene cloning Gene Expression Regulation Genes. Genome Genomic sequence Insect Proteins Molecular and cellular biology Molecular genetics Molecular Sequence Data Molting hormone Protein Biosynthesis Proteins - genetics Restriction Mapping Transcription, Genetic Transcriptional regulation |
| title | Structure and expression of gene coding for a pupal cuticle protein of Bombyx mori |
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