Differential Roles of PIN1 and PIN2 in Root Meristem Maintenance Under Low-B Conditions in Arabidopsis thaliana
Boron (B) is an essential element for plants; its deficiency causes rapid cessation of root elongation. In addition, B influences auxin accumulation in plants. To assess the importance of auxin transport in B-dependent root elongation, Arabidopsis thaliana pin1-pin4 mutants were grown under low-B co...
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| Veröffentlicht in: | Plant and cell physiology 2015-06, Vol.56 (6), p.1205-1214 |
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| container_title | Plant and cell physiology |
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| creator | Li, Ke Kamiya, Takehiro Fujiwara, Toru |
| description | Boron (B) is an essential element for plants; its deficiency causes rapid cessation of root elongation. In addition, B influences auxin accumulation in plants. To assess the importance of auxin transport in B-dependent root elongation, Arabidopsis thaliana pin1-pin4 mutants were grown under low-B conditions. Among them, only the pin2/eir1-1 mutant showed a significantly shorter root under low-B conditions than under control conditions. Moreover, the root meristem size of pin2/eir1-1 was reduced under low-B conditions. Among the PIN-FORMED (PIN) family, PIN1 and PIN2 are important for root meristem growth/maintenance under normal conditions. To investigate the differential response of pin1 and pin2 mutants under low-B conditions, the effect of low-B on PIN1-green fluorescent protein (GFP) and PIN2-GFP accumulation and localization was examined. Low-B did not affect PIN2-GFP, while it reduced the accumulation of PIN1-GFP. Moreover, no signal from DII-VENUS, an auxin sensor, was detected under the low-B condition in the stele of wild-type root meristems. Taken together, these results indicate that under low-B conditions PIN1 is down-regulated and PIN2 plays an important role in root meristem maintenance. |
| doi_str_mv | 10.1093/pcp/pcv047 |
| format | Article |
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In addition, B influences auxin accumulation in plants. To assess the importance of auxin transport in B-dependent root elongation, Arabidopsis thaliana pin1-pin4 mutants were grown under low-B conditions. Among them, only the pin2/eir1-1 mutant showed a significantly shorter root under low-B conditions than under control conditions. Moreover, the root meristem size of pin2/eir1-1 was reduced under low-B conditions. Among the PIN-FORMED (PIN) family, PIN1 and PIN2 are important for root meristem growth/maintenance under normal conditions. To investigate the differential response of pin1 and pin2 mutants under low-B conditions, the effect of low-B on PIN1-green fluorescent protein (GFP) and PIN2-GFP accumulation and localization was examined. Low-B did not affect PIN2-GFP, while it reduced the accumulation of PIN1-GFP. Moreover, no signal from DII-VENUS, an auxin sensor, was detected under the low-B condition in the stele of wild-type root meristems. Taken together, these results indicate that under low-B conditions PIN1 is down-regulated and PIN2 plays an important role in root meristem maintenance.</description><identifier>ISSN: 0032-0781</identifier><identifier>EISSN: 1471-9053</identifier><identifier>DOI: 10.1093/pcp/pcv047</identifier><identifier>PMID: 25814435</identifier><language>eng</language><publisher>Japan</publisher><subject>Arabidopsis - drug effects ; Arabidopsis - genetics ; Arabidopsis - growth & development ; Arabidopsis - metabolism ; Arabidopsis Proteins - genetics ; Arabidopsis Proteins - metabolism ; Boron - pharmacology ; Gene Expression Regulation, Plant - drug effects ; Green Fluorescent Proteins - metabolism ; Indoleacetic Acids - metabolism ; Membrane Transport Proteins - genetics ; Membrane Transport Proteins - metabolism ; Meristem - drug effects ; Meristem - metabolism ; Mutation - genetics ; Organ Size - drug effects</subject><ispartof>Plant and cell physiology, 2015-06, Vol.56 (6), p.1205-1214</ispartof><rights>The Author 2015. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oup.com.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c389t-53b62a9c757a64f077dcef94e34e47eb8f36548da6450d47d2b9f97d6d2d57513</citedby><cites>FETCH-LOGICAL-c389t-53b62a9c757a64f077dcef94e34e47eb8f36548da6450d47d2b9f97d6d2d57513</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25814435$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Ke</creatorcontrib><creatorcontrib>Kamiya, Takehiro</creatorcontrib><creatorcontrib>Fujiwara, Toru</creatorcontrib><title>Differential Roles of PIN1 and PIN2 in Root Meristem Maintenance Under Low-B Conditions in Arabidopsis thaliana</title><title>Plant and cell physiology</title><addtitle>Plant Cell Physiol</addtitle><description>Boron (B) is an essential element for plants; its deficiency causes rapid cessation of root elongation. In addition, B influences auxin accumulation in plants. To assess the importance of auxin transport in B-dependent root elongation, Arabidopsis thaliana pin1-pin4 mutants were grown under low-B conditions. Among them, only the pin2/eir1-1 mutant showed a significantly shorter root under low-B conditions than under control conditions. Moreover, the root meristem size of pin2/eir1-1 was reduced under low-B conditions. Among the PIN-FORMED (PIN) family, PIN1 and PIN2 are important for root meristem growth/maintenance under normal conditions. To investigate the differential response of pin1 and pin2 mutants under low-B conditions, the effect of low-B on PIN1-green fluorescent protein (GFP) and PIN2-GFP accumulation and localization was examined. Low-B did not affect PIN2-GFP, while it reduced the accumulation of PIN1-GFP. Moreover, no signal from DII-VENUS, an auxin sensor, was detected under the low-B condition in the stele of wild-type root meristems. Taken together, these results indicate that under low-B conditions PIN1 is down-regulated and PIN2 plays an important role in root meristem maintenance.</description><subject>Arabidopsis - drug effects</subject><subject>Arabidopsis - genetics</subject><subject>Arabidopsis - growth & development</subject><subject>Arabidopsis - metabolism</subject><subject>Arabidopsis Proteins - genetics</subject><subject>Arabidopsis Proteins - metabolism</subject><subject>Boron - pharmacology</subject><subject>Gene Expression Regulation, Plant - drug effects</subject><subject>Green Fluorescent Proteins - metabolism</subject><subject>Indoleacetic Acids - metabolism</subject><subject>Membrane Transport Proteins - genetics</subject><subject>Membrane Transport Proteins - metabolism</subject><subject>Meristem - drug effects</subject><subject>Meristem - metabolism</subject><subject>Mutation - genetics</subject><subject>Organ Size - drug effects</subject><issn>0032-0781</issn><issn>1471-9053</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kE1LxDAQhoMo7rp68QdIjiJUkyZp2uO6fsKuirjnkjYTjLRJTbqK_96WXT0M88L7zBwehE4puaSkYFdd3Q3zRbjcQ1PKJU0KItg-mhLC0oTInE7QUYwfhAyZkUM0SUVOOWdiivyNNQYCuN6qBr_6BiL2Br88PlGsnB5Diq0bGt_jFQQbe2jxSlnXg1OuBrx2GgJe-u_kGi-807a33sXxZh5UZbXvoo24f1eNVU4dowOjmggnuz1D67vbt8VDsny-f1zMl0nN8qJPBKuyVBW1FFJl3BApdQ2m4MA4cAlVblgmeK6HUhDNpU6rwhRSZzrVQgrKZuh8-7cL_nMDsS9bG2toGuXAb2JJs1yyjIpiRC-2aB18jAFM2QXbqvBTUlKOgstBcLkVPMBnu7-bqgX9j_4ZZb-UnHax</recordid><startdate>20150601</startdate><enddate>20150601</enddate><creator>Li, Ke</creator><creator>Kamiya, Takehiro</creator><creator>Fujiwara, Toru</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20150601</creationdate><title>Differential Roles of PIN1 and PIN2 in Root Meristem Maintenance Under Low-B Conditions in Arabidopsis thaliana</title><author>Li, Ke ; Kamiya, Takehiro ; Fujiwara, Toru</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c389t-53b62a9c757a64f077dcef94e34e47eb8f36548da6450d47d2b9f97d6d2d57513</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Arabidopsis - drug effects</topic><topic>Arabidopsis - genetics</topic><topic>Arabidopsis - growth & development</topic><topic>Arabidopsis - metabolism</topic><topic>Arabidopsis Proteins - genetics</topic><topic>Arabidopsis Proteins - metabolism</topic><topic>Boron - pharmacology</topic><topic>Gene Expression Regulation, Plant - drug effects</topic><topic>Green Fluorescent Proteins - metabolism</topic><topic>Indoleacetic Acids - metabolism</topic><topic>Membrane Transport Proteins - genetics</topic><topic>Membrane Transport Proteins - metabolism</topic><topic>Meristem - drug effects</topic><topic>Meristem - metabolism</topic><topic>Mutation - genetics</topic><topic>Organ Size - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Ke</creatorcontrib><creatorcontrib>Kamiya, Takehiro</creatorcontrib><creatorcontrib>Fujiwara, Toru</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Plant and cell physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Ke</au><au>Kamiya, Takehiro</au><au>Fujiwara, Toru</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differential Roles of PIN1 and PIN2 in Root Meristem Maintenance Under Low-B Conditions in Arabidopsis thaliana</atitle><jtitle>Plant and cell physiology</jtitle><addtitle>Plant Cell Physiol</addtitle><date>2015-06-01</date><risdate>2015</risdate><volume>56</volume><issue>6</issue><spage>1205</spage><epage>1214</epage><pages>1205-1214</pages><issn>0032-0781</issn><eissn>1471-9053</eissn><abstract>Boron (B) is an essential element for plants; its deficiency causes rapid cessation of root elongation. In addition, B influences auxin accumulation in plants. To assess the importance of auxin transport in B-dependent root elongation, Arabidopsis thaliana pin1-pin4 mutants were grown under low-B conditions. Among them, only the pin2/eir1-1 mutant showed a significantly shorter root under low-B conditions than under control conditions. Moreover, the root meristem size of pin2/eir1-1 was reduced under low-B conditions. Among the PIN-FORMED (PIN) family, PIN1 and PIN2 are important for root meristem growth/maintenance under normal conditions. To investigate the differential response of pin1 and pin2 mutants under low-B conditions, the effect of low-B on PIN1-green fluorescent protein (GFP) and PIN2-GFP accumulation and localization was examined. Low-B did not affect PIN2-GFP, while it reduced the accumulation of PIN1-GFP. Moreover, no signal from DII-VENUS, an auxin sensor, was detected under the low-B condition in the stele of wild-type root meristems. Taken together, these results indicate that under low-B conditions PIN1 is down-regulated and PIN2 plays an important role in root meristem maintenance.</abstract><cop>Japan</cop><pmid>25814435</pmid><doi>10.1093/pcp/pcv047</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Plant and cell physiology, 2015-06, Vol.56 (6), p.1205-1214 |
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| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current); EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Arabidopsis - drug effects Arabidopsis - genetics Arabidopsis - growth & development Arabidopsis - metabolism Arabidopsis Proteins - genetics Arabidopsis Proteins - metabolism Boron - pharmacology Gene Expression Regulation, Plant - drug effects Green Fluorescent Proteins - metabolism Indoleacetic Acids - metabolism Membrane Transport Proteins - genetics Membrane Transport Proteins - metabolism Meristem - drug effects Meristem - metabolism Mutation - genetics Organ Size - drug effects |
| title | Differential Roles of PIN1 and PIN2 in Root Meristem Maintenance Under Low-B Conditions in Arabidopsis thaliana |
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