Effects of Water on the Single-Chain Elasticity of Poly(U) RNA

Water, the dominant component under the physiological condition, is a complicated solvent which greatly affects the properties of solute molecules. Here, we utilize atomic force microscope-based single-molecule force spectroscopy to study the influence of water on the single-molecule elasticity of a...

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Veröffentlicht in:Langmuir 2015-06, Vol.31 (22), p.6107-6113
Hauptverfasser: Luo, Zhonglong, Cheng, Bo, Cui, Shuxun
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description Water, the dominant component under the physiological condition, is a complicated solvent which greatly affects the properties of solute molecules. Here, we utilize atomic force microscope-based single-molecule force spectroscopy to study the influence of water on the single-molecule elasticity of an unstructured single-stranded RNA (poly­(U)). In nonpolar solvents, RNA presents its inherent elasticity, which is consistent with the theoretical single-chain elasticity calculated by quantum mechanics calculations. In aqueous buffers, however, an additional energy of 1.88 kJ/mol·base is needed for the stretching of the ssRNA chain. This energy is consumed by the bound water rearrangement ( E w) during chain elongation. Further experimental results indicate that the E w value is uncorrelated to the salt concentrations and stretching velocity. The results obtained in an 8 M guanidine·HCl solution provide more evidence that the bound water molecules around RNA give rise to the observed deviation between aqueous and nonaqueous environments. Compared to synthetic water-soluble polymers, the value of E w of RNA is much lower. The weak interference of water is supposed to be the precondition for the RNA secondary structure to exist in aqueous solution.
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subjects Elasticity
Poly U - chemistry
Quantum Theory
RNA - chemistry
Water - chemistry
title Effects of Water on the Single-Chain Elasticity of Poly(U) RNA
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