Loss of diversity within Mycoplasma bovis isolates collected in France from bovines with respiratory diseases over the last 35 years

Mycoplasma (M.) bovis has recently emerged as a major, worldwide etiological agent of bovine respiratory diseases leading to huge economic losses mainly due to high morbidity and mortality as well as poor growth rates. The spread of M. bovis infections between different animals, herds, regions or co...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Infection, genetics and evolution genetics and evolution, 2015-07, Vol.33, p.118-126
Hauptverfasser: Becker, Claire A M, Thibault, François M, Arcangioli, Marie-Anne, Tardy, Florence
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 126
container_issue
container_start_page 118
container_title Infection, genetics and evolution
container_volume 33
creator Becker, Claire A M
Thibault, François M
Arcangioli, Marie-Anne
Tardy, Florence
description Mycoplasma (M.) bovis has recently emerged as a major, worldwide etiological agent of bovine respiratory diseases leading to huge economic losses mainly due to high morbidity and mortality as well as poor growth rates. The spread of M. bovis infections between different animals, herds, regions or countries has been often reported to be connected to the movement of animals. However, despite recent considerable efforts, no universal subtyping method is yet available to trace M. bovis isolates circulation at an international scale. Moreover in France, the overall population diversity of M. bovis isolates has not been assessed since the early 1990s. This study was conducted to fill in these gaps. The genotypic diversity between sixty isolates collected in France over the last 35 years was assessed using two molecular subtyping methods that addressed either the long-term epidemiological relationships (Multi Locus Sequence Typing, MLST) or the genetic microvariations (Multiple Locus VNTR Analysis, MLVA) between isolates. Phenotypic diversity was also analyzed by using Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) to compare the main protein patterns of isolates. All proposed subtyping approaches were optimized and led to the same pattern in the French M. bovis population that consisted of two clusters, the first one comprising isolates collected before 2000 and the second, those collected after 2000. Recent strains were further shown to be more homogeneous than older ones, which is consistent with the spread of a single clone throughout the country. Because this spread was concomitant with the emergence of multiresistant M. bovis isolates, several hypotheses are discussed to explain the homogeneity of M. bovis isolates in France, even though the M. bovis species is fully equipped to generate diversity.
doi_str_mv 10.1016/j.meegid.2015.04.019
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1687348112</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1687348112</sourcerecordid><originalsourceid>FETCH-LOGICAL-c237t-6abc8a2d82d390c9c550a8161a8b34a2a33c759275831b910a1d1b56d05779583</originalsourceid><addsrcrecordid>eNo9kMFuEzEQhq0KREvhDRDykUsWj71ee4-ooi1SKi5wtma9TutoNw4ep2jvPDgOCT3NaOabf6SPsQ8gGhDQfd42cwiPcWykAN2IthHQX7Ar0J1ZGanNq3MPqrWX7C3RVggwQto37FLqHhRoe8X-rBMRTxs-xueQKZaF_47lKe74w-LTfkKakQ_pORKPlCYsgbhP0xR8CSOv2G3GnQ98k9P8j9tV4JjAc6B9zFhSXmo4BaS6SfUJL0-B1-DCleZLwEzv2OsNThTen-s1-3n79cfN_Wr9_e7bzZf1yktlyqrDwVuUo5Wj6oXvvdYCLXSAdlAtSlTKG91Lo62CoQeBMMKgu1FoY_o6vGafTrn7nH4dAhU3R_JhmnAX0oEcdNZUWwCyou0J9bkKymHj9jnOmBcHwh39u607-XdH_060rvqvZx_PHw7DHMaXo__C1V9wUYSf</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1687348112</pqid></control><display><type>article</type><title>Loss of diversity within Mycoplasma bovis isolates collected in France from bovines with respiratory diseases over the last 35 years</title><source>MEDLINE</source><source>ScienceDirect</source><creator>Becker, Claire A M ; Thibault, François M ; Arcangioli, Marie-Anne ; Tardy, Florence</creator><creatorcontrib>Becker, Claire A M ; Thibault, François M ; Arcangioli, Marie-Anne ; Tardy, Florence</creatorcontrib><description>Mycoplasma (M.) bovis has recently emerged as a major, worldwide etiological agent of bovine respiratory diseases leading to huge economic losses mainly due to high morbidity and mortality as well as poor growth rates. The spread of M. bovis infections between different animals, herds, regions or countries has been often reported to be connected to the movement of animals. However, despite recent considerable efforts, no universal subtyping method is yet available to trace M. bovis isolates circulation at an international scale. Moreover in France, the overall population diversity of M. bovis isolates has not been assessed since the early 1990s. This study was conducted to fill in these gaps. The genotypic diversity between sixty isolates collected in France over the last 35 years was assessed using two molecular subtyping methods that addressed either the long-term epidemiological relationships (Multi Locus Sequence Typing, MLST) or the genetic microvariations (Multiple Locus VNTR Analysis, MLVA) between isolates. Phenotypic diversity was also analyzed by using Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) to compare the main protein patterns of isolates. All proposed subtyping approaches were optimized and led to the same pattern in the French M. bovis population that consisted of two clusters, the first one comprising isolates collected before 2000 and the second, those collected after 2000. Recent strains were further shown to be more homogeneous than older ones, which is consistent with the spread of a single clone throughout the country. Because this spread was concomitant with the emergence of multiresistant M. bovis isolates, several hypotheses are discussed to explain the homogeneity of M. bovis isolates in France, even though the M. bovis species is fully equipped to generate diversity.</description><identifier>ISSN: 1567-1348</identifier><identifier>EISSN: 1567-7257</identifier><identifier>DOI: 10.1016/j.meegid.2015.04.019</identifier><identifier>PMID: 25913158</identifier><language>eng</language><publisher>Netherlands</publisher><subject>Animals ; Cattle ; Cattle Diseases - epidemiology ; Cattle Diseases - history ; Cattle Diseases - microbiology ; France ; Genetic Variation ; Genotype ; History, 20th Century ; History, 21st Century ; Minisatellite Repeats ; Multilocus Sequence Typing ; Mycoplasma bovis - classification ; Mycoplasma bovis - genetics ; Mycoplasma Infections - veterinary ; Phylogeny</subject><ispartof>Infection, genetics and evolution, 2015-07, Vol.33, p.118-126</ispartof><rights>Copyright © 2015 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c237t-6abc8a2d82d390c9c550a8161a8b34a2a33c759275831b910a1d1b56d05779583</citedby><cites>FETCH-LOGICAL-c237t-6abc8a2d82d390c9c550a8161a8b34a2a33c759275831b910a1d1b56d05779583</cites><orcidid>0000-0001-5830-2915</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25913158$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Becker, Claire A M</creatorcontrib><creatorcontrib>Thibault, François M</creatorcontrib><creatorcontrib>Arcangioli, Marie-Anne</creatorcontrib><creatorcontrib>Tardy, Florence</creatorcontrib><title>Loss of diversity within Mycoplasma bovis isolates collected in France from bovines with respiratory diseases over the last 35 years</title><title>Infection, genetics and evolution</title><addtitle>Infect Genet Evol</addtitle><description>Mycoplasma (M.) bovis has recently emerged as a major, worldwide etiological agent of bovine respiratory diseases leading to huge economic losses mainly due to high morbidity and mortality as well as poor growth rates. The spread of M. bovis infections between different animals, herds, regions or countries has been often reported to be connected to the movement of animals. However, despite recent considerable efforts, no universal subtyping method is yet available to trace M. bovis isolates circulation at an international scale. Moreover in France, the overall population diversity of M. bovis isolates has not been assessed since the early 1990s. This study was conducted to fill in these gaps. The genotypic diversity between sixty isolates collected in France over the last 35 years was assessed using two molecular subtyping methods that addressed either the long-term epidemiological relationships (Multi Locus Sequence Typing, MLST) or the genetic microvariations (Multiple Locus VNTR Analysis, MLVA) between isolates. Phenotypic diversity was also analyzed by using Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) to compare the main protein patterns of isolates. All proposed subtyping approaches were optimized and led to the same pattern in the French M. bovis population that consisted of two clusters, the first one comprising isolates collected before 2000 and the second, those collected after 2000. Recent strains were further shown to be more homogeneous than older ones, which is consistent with the spread of a single clone throughout the country. Because this spread was concomitant with the emergence of multiresistant M. bovis isolates, several hypotheses are discussed to explain the homogeneity of M. bovis isolates in France, even though the M. bovis species is fully equipped to generate diversity.</description><subject>Animals</subject><subject>Cattle</subject><subject>Cattle Diseases - epidemiology</subject><subject>Cattle Diseases - history</subject><subject>Cattle Diseases - microbiology</subject><subject>France</subject><subject>Genetic Variation</subject><subject>Genotype</subject><subject>History, 20th Century</subject><subject>History, 21st Century</subject><subject>Minisatellite Repeats</subject><subject>Multilocus Sequence Typing</subject><subject>Mycoplasma bovis - classification</subject><subject>Mycoplasma bovis - genetics</subject><subject>Mycoplasma Infections - veterinary</subject><subject>Phylogeny</subject><issn>1567-1348</issn><issn>1567-7257</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kMFuEzEQhq0KREvhDRDykUsWj71ee4-ooi1SKi5wtma9TutoNw4ep2jvPDgOCT3NaOabf6SPsQ8gGhDQfd42cwiPcWykAN2IthHQX7Ar0J1ZGanNq3MPqrWX7C3RVggwQto37FLqHhRoe8X-rBMRTxs-xueQKZaF_47lKe74w-LTfkKakQ_pORKPlCYsgbhP0xR8CSOv2G3GnQ98k9P8j9tV4JjAc6B9zFhSXmo4BaS6SfUJL0-B1-DCleZLwEzv2OsNThTen-s1-3n79cfN_Wr9_e7bzZf1yktlyqrDwVuUo5Wj6oXvvdYCLXSAdlAtSlTKG91Lo62CoQeBMMKgu1FoY_o6vGafTrn7nH4dAhU3R_JhmnAX0oEcdNZUWwCyou0J9bkKymHj9jnOmBcHwh39u607-XdH_060rvqvZx_PHw7DHMaXo__C1V9wUYSf</recordid><startdate>201507</startdate><enddate>201507</enddate><creator>Becker, Claire A M</creator><creator>Thibault, François M</creator><creator>Arcangioli, Marie-Anne</creator><creator>Tardy, Florence</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-5830-2915</orcidid></search><sort><creationdate>201507</creationdate><title>Loss of diversity within Mycoplasma bovis isolates collected in France from bovines with respiratory diseases over the last 35 years</title><author>Becker, Claire A M ; Thibault, François M ; Arcangioli, Marie-Anne ; Tardy, Florence</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c237t-6abc8a2d82d390c9c550a8161a8b34a2a33c759275831b910a1d1b56d05779583</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Animals</topic><topic>Cattle</topic><topic>Cattle Diseases - epidemiology</topic><topic>Cattle Diseases - history</topic><topic>Cattle Diseases - microbiology</topic><topic>France</topic><topic>Genetic Variation</topic><topic>Genotype</topic><topic>History, 20th Century</topic><topic>History, 21st Century</topic><topic>Minisatellite Repeats</topic><topic>Multilocus Sequence Typing</topic><topic>Mycoplasma bovis - classification</topic><topic>Mycoplasma bovis - genetics</topic><topic>Mycoplasma Infections - veterinary</topic><topic>Phylogeny</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Becker, Claire A M</creatorcontrib><creatorcontrib>Thibault, François M</creatorcontrib><creatorcontrib>Arcangioli, Marie-Anne</creatorcontrib><creatorcontrib>Tardy, Florence</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Infection, genetics and evolution</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Becker, Claire A M</au><au>Thibault, François M</au><au>Arcangioli, Marie-Anne</au><au>Tardy, Florence</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Loss of diversity within Mycoplasma bovis isolates collected in France from bovines with respiratory diseases over the last 35 years</atitle><jtitle>Infection, genetics and evolution</jtitle><addtitle>Infect Genet Evol</addtitle><date>2015-07</date><risdate>2015</risdate><volume>33</volume><spage>118</spage><epage>126</epage><pages>118-126</pages><issn>1567-1348</issn><eissn>1567-7257</eissn><abstract>Mycoplasma (M.) bovis has recently emerged as a major, worldwide etiological agent of bovine respiratory diseases leading to huge economic losses mainly due to high morbidity and mortality as well as poor growth rates. The spread of M. bovis infections between different animals, herds, regions or countries has been often reported to be connected to the movement of animals. However, despite recent considerable efforts, no universal subtyping method is yet available to trace M. bovis isolates circulation at an international scale. Moreover in France, the overall population diversity of M. bovis isolates has not been assessed since the early 1990s. This study was conducted to fill in these gaps. The genotypic diversity between sixty isolates collected in France over the last 35 years was assessed using two molecular subtyping methods that addressed either the long-term epidemiological relationships (Multi Locus Sequence Typing, MLST) or the genetic microvariations (Multiple Locus VNTR Analysis, MLVA) between isolates. Phenotypic diversity was also analyzed by using Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) to compare the main protein patterns of isolates. All proposed subtyping approaches were optimized and led to the same pattern in the French M. bovis population that consisted of two clusters, the first one comprising isolates collected before 2000 and the second, those collected after 2000. Recent strains were further shown to be more homogeneous than older ones, which is consistent with the spread of a single clone throughout the country. Because this spread was concomitant with the emergence of multiresistant M. bovis isolates, several hypotheses are discussed to explain the homogeneity of M. bovis isolates in France, even though the M. bovis species is fully equipped to generate diversity.</abstract><cop>Netherlands</cop><pmid>25913158</pmid><doi>10.1016/j.meegid.2015.04.019</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0001-5830-2915</orcidid></addata></record>
fulltext fulltext
identifier ISSN: 1567-1348
ispartof Infection, genetics and evolution, 2015-07, Vol.33, p.118-126
issn 1567-1348
1567-7257
language eng
recordid cdi_proquest_miscellaneous_1687348112
source MEDLINE; ScienceDirect
subjects Animals
Cattle
Cattle Diseases - epidemiology
Cattle Diseases - history
Cattle Diseases - microbiology
France
Genetic Variation
Genotype
History, 20th Century
History, 21st Century
Minisatellite Repeats
Multilocus Sequence Typing
Mycoplasma bovis - classification
Mycoplasma bovis - genetics
Mycoplasma Infections - veterinary
Phylogeny
title Loss of diversity within Mycoplasma bovis isolates collected in France from bovines with respiratory diseases over the last 35 years
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T08%3A02%3A41IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Loss%20of%20diversity%20within%20Mycoplasma%20bovis%20isolates%20collected%20in%20France%20from%20bovines%20with%20respiratory%20diseases%20over%20the%20last%2035%20years&rft.jtitle=Infection,%20genetics%20and%20evolution&rft.au=Becker,%20Claire%20A%20M&rft.date=2015-07&rft.volume=33&rft.spage=118&rft.epage=126&rft.pages=118-126&rft.issn=1567-1348&rft.eissn=1567-7257&rft_id=info:doi/10.1016/j.meegid.2015.04.019&rft_dat=%3Cproquest_cross%3E1687348112%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1687348112&rft_id=info:pmid/25913158&rfr_iscdi=true