Down-regulation of P4501A1 and P4501A2 mRNA expression in isolated hepatocytes by oxidative stress
We have previously shown that the inflammatory mediator interleukin-1 suppressed transcription of CYP1A1 and CYP1A2 mRNAs (Barker, C.W., Fagan, J.B., and Pasco, D.S. (1992) J. Biol. Chem. 267, 8050-8055). Since many of the actions of inflammatory mediators are mimicked by oxidative stress, we treate...
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| Veröffentlicht in: | The Journal of biological chemistry 1994-02, Vol.269 (6), p.3985-3990 |
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| creator | BARKER, C. W FAGAN, J. B PASCO, D. S |
| description | We have previously shown that the inflammatory mediator interleukin-1 suppressed transcription of CYP1A1 and CYP1A2 mRNAs
(Barker, C.W., Fagan, J.B., and Pasco, D.S. (1992) J. Biol. Chem. 267, 8050-8055). Since many of the actions of inflammatory
mediators are mimicked by oxidative stress, we treated isolated hepatocytes with 0.25-1.0 mM H2O2 to determine whether expression
of these genes is also modulated by oxidative stress. Inducer-dependent accumulation of CYP1A1 and CYP1A2 mRNAs were maximally
reduced approximately 50 and 70%, respectively, by 1.0 mM H2O2. Run-on transcription analysis suggested that the effect of
H2O2 was mediated transcriptionally. The reduction in CYP1A mRNA levels was not due to a reduction in the levels of all mRNAs
due to some general toxic effect since H2O2 did not reduce glyceraldehyde-3-phosphate dehydrogenase, alpha-tubulin, beta-fibrinogen,
or albumin mRNA levels, and did not increase lactate dehydrogenase released into the medium. Insulin-mimicked H2O2 action,
reducing the expression of both mRNAs, and N-acetylcysteine, which increases intracellular glutathione levels, completely
reversed the insulin effect on both mRNAs and the H2O2 effect on CYP1A1 mRNA, but only partially reversed the H2O2 effect
on CYP1A2 mRNA. This study indicates that the CYP1A1 and CYP1A2 genes are responsive to oxidative stress and that the majority
of this responsiveness can be modified by cellular redox potential. |
| doi_str_mv | 10.1016/s0021-9258(17)41731-5 |
| format | Article |
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(Barker, C.W., Fagan, J.B., and Pasco, D.S. (1992) J. Biol. Chem. 267, 8050-8055). Since many of the actions of inflammatory
mediators are mimicked by oxidative stress, we treated isolated hepatocytes with 0.25-1.0 mM H2O2 to determine whether expression
of these genes is also modulated by oxidative stress. Inducer-dependent accumulation of CYP1A1 and CYP1A2 mRNAs were maximally
reduced approximately 50 and 70%, respectively, by 1.0 mM H2O2. Run-on transcription analysis suggested that the effect of
H2O2 was mediated transcriptionally. The reduction in CYP1A mRNA levels was not due to a reduction in the levels of all mRNAs
due to some general toxic effect since H2O2 did not reduce glyceraldehyde-3-phosphate dehydrogenase, alpha-tubulin, beta-fibrinogen,
or albumin mRNA levels, and did not increase lactate dehydrogenase released into the medium. Insulin-mimicked H2O2 action,
reducing the expression of both mRNAs, and N-acetylcysteine, which increases intracellular glutathione levels, completely
reversed the insulin effect on both mRNAs and the H2O2 effect on CYP1A1 mRNA, but only partially reversed the H2O2 effect
on CYP1A2 mRNA. This study indicates that the CYP1A1 and CYP1A2 genes are responsive to oxidative stress and that the majority
of this responsiveness can be modified by cellular redox potential.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1016/s0021-9258(17)41731-5</identifier><identifier>PMID: 8307954</identifier><identifier>CODEN: JBCHA3</identifier><language>eng</language><publisher>Bethesda, MD: American Society for Biochemistry and Molecular Biology</publisher><subject>Animals ; Benzoflavones - pharmacology ; beta-Naphthoflavone ; Biological and medical sciences ; Cell Survival - drug effects ; Cytochrome P-450 Enzyme System - genetics ; Cytochrome P-450 Enzyme System - metabolism ; Fundamental and applied biological sciences. Psychology ; Gene Expression ; Genes, jun ; Glutathione - metabolism ; Hydrogen Peroxide - pharmacology ; Insulin - pharmacology ; Liver - metabolism ; Male ; Molecular and cellular biology ; Molecular genetics ; Oxidation-Reduction ; Rats ; Rats, Sprague-Dawley ; RNA, Messenger - genetics ; Stress, Physiological - enzymology ; Transcription, Genetic - drug effects ; Transcription. Transcription factor. Splicing. Rna processing</subject><ispartof>The Journal of biological chemistry, 1994-02, Vol.269 (6), p.3985-3990</ispartof><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4195-c079957089ee27358c2279231891c7a9a45eb4992ce95e32249b143717849043</citedby><cites>FETCH-LOGICAL-c4195-c079957089ee27358c2279231891c7a9a45eb4992ce95e32249b143717849043</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4045262$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8307954$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>BARKER, C. W</creatorcontrib><creatorcontrib>FAGAN, J. B</creatorcontrib><creatorcontrib>PASCO, D. S</creatorcontrib><title>Down-regulation of P4501A1 and P4501A2 mRNA expression in isolated hepatocytes by oxidative stress</title><title>The Journal of biological chemistry</title><addtitle>J Biol Chem</addtitle><description>We have previously shown that the inflammatory mediator interleukin-1 suppressed transcription of CYP1A1 and CYP1A2 mRNAs
(Barker, C.W., Fagan, J.B., and Pasco, D.S. (1992) J. Biol. Chem. 267, 8050-8055). Since many of the actions of inflammatory
mediators are mimicked by oxidative stress, we treated isolated hepatocytes with 0.25-1.0 mM H2O2 to determine whether expression
of these genes is also modulated by oxidative stress. Inducer-dependent accumulation of CYP1A1 and CYP1A2 mRNAs were maximally
reduced approximately 50 and 70%, respectively, by 1.0 mM H2O2. Run-on transcription analysis suggested that the effect of
H2O2 was mediated transcriptionally. The reduction in CYP1A mRNA levels was not due to a reduction in the levels of all mRNAs
due to some general toxic effect since H2O2 did not reduce glyceraldehyde-3-phosphate dehydrogenase, alpha-tubulin, beta-fibrinogen,
or albumin mRNA levels, and did not increase lactate dehydrogenase released into the medium. Insulin-mimicked H2O2 action,
reducing the expression of both mRNAs, and N-acetylcysteine, which increases intracellular glutathione levels, completely
reversed the insulin effect on both mRNAs and the H2O2 effect on CYP1A1 mRNA, but only partially reversed the H2O2 effect
on CYP1A2 mRNA. This study indicates that the CYP1A1 and CYP1A2 genes are responsive to oxidative stress and that the majority
of this responsiveness can be modified by cellular redox potential.</description><subject>Animals</subject><subject>Benzoflavones - pharmacology</subject><subject>beta-Naphthoflavone</subject><subject>Biological and medical sciences</subject><subject>Cell Survival - drug effects</subject><subject>Cytochrome P-450 Enzyme System - genetics</subject><subject>Cytochrome P-450 Enzyme System - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression</subject><subject>Genes, jun</subject><subject>Glutathione - metabolism</subject><subject>Hydrogen Peroxide - pharmacology</subject><subject>Insulin - pharmacology</subject><subject>Liver - metabolism</subject><subject>Male</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Oxidation-Reduction</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>RNA, Messenger - genetics</subject><subject>Stress, Physiological - enzymology</subject><subject>Transcription, Genetic - drug effects</subject><subject>Transcription. Transcription factor. Splicing. Rna processing</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kF1rFDEUhoModVv9CYUgInoxNScfk-RyqVaFYkV74V3IZM92IzOTbTLbdv-9M91hQyAHzvOeEx5CzoFdAIP6c2GMQ2W5Mh9Bf5KgBVTqBVkAM6ISCv6-JIsj8pqclvKPjUdaOCEnRjBtlVyQ5kt67KuMd7vWDzH1NK3pL6kYLIH6fjXXnHa_fy4pPm0zljJhcbwljRlc0Q1u_ZDCfsBCmz1NT3E1znpAWoYJf0NerX1b8O38npHbq6-3l9-r65tvPy6X11WQYFUVxh9ZpZmxiFwLZQLn2nIBxkLQ3nqpsJHW8oBWoeBc2gak0KCNtEyKM_LhMHab0_0Oy-C6WAK2re8x7YqD2tTGcD6C6gCGnErJuHbbHDuf9w6Ym9S6P5M3N3lzoN2zWqfG3Pm8YNd0uDqmZpdj__3c9yX4dp19H2I5YpJJxetp_bsDtol3m8eY0TUxhQ12jtfW1U5Yo8R_0xeJEg</recordid><startdate>19940211</startdate><enddate>19940211</enddate><creator>BARKER, C. W</creator><creator>FAGAN, J. B</creator><creator>PASCO, D. S</creator><general>American Society for Biochemistry and Molecular Biology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope></search><sort><creationdate>19940211</creationdate><title>Down-regulation of P4501A1 and P4501A2 mRNA expression in isolated hepatocytes by oxidative stress</title><author>BARKER, C. W ; FAGAN, J. B ; PASCO, D. S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4195-c079957089ee27358c2279231891c7a9a45eb4992ce95e32249b143717849043</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Animals</topic><topic>Benzoflavones - pharmacology</topic><topic>beta-Naphthoflavone</topic><topic>Biological and medical sciences</topic><topic>Cell Survival - drug effects</topic><topic>Cytochrome P-450 Enzyme System - genetics</topic><topic>Cytochrome P-450 Enzyme System - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression</topic><topic>Genes, jun</topic><topic>Glutathione - metabolism</topic><topic>Hydrogen Peroxide - pharmacology</topic><topic>Insulin - pharmacology</topic><topic>Liver - metabolism</topic><topic>Male</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Oxidation-Reduction</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>RNA, Messenger - genetics</topic><topic>Stress, Physiological - enzymology</topic><topic>Transcription, Genetic - drug effects</topic><topic>Transcription. Transcription factor. Splicing. Rna processing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>BARKER, C. W</creatorcontrib><creatorcontrib>FAGAN, J. B</creatorcontrib><creatorcontrib>PASCO, D. S</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>BARKER, C. W</au><au>FAGAN, J. B</au><au>PASCO, D. S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Down-regulation of P4501A1 and P4501A2 mRNA expression in isolated hepatocytes by oxidative stress</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>1994-02-11</date><risdate>1994</risdate><volume>269</volume><issue>6</issue><spage>3985</spage><epage>3990</epage><pages>3985-3990</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><coden>JBCHA3</coden><abstract>We have previously shown that the inflammatory mediator interleukin-1 suppressed transcription of CYP1A1 and CYP1A2 mRNAs
(Barker, C.W., Fagan, J.B., and Pasco, D.S. (1992) J. Biol. Chem. 267, 8050-8055). Since many of the actions of inflammatory
mediators are mimicked by oxidative stress, we treated isolated hepatocytes with 0.25-1.0 mM H2O2 to determine whether expression
of these genes is also modulated by oxidative stress. Inducer-dependent accumulation of CYP1A1 and CYP1A2 mRNAs were maximally
reduced approximately 50 and 70%, respectively, by 1.0 mM H2O2. Run-on transcription analysis suggested that the effect of
H2O2 was mediated transcriptionally. The reduction in CYP1A mRNA levels was not due to a reduction in the levels of all mRNAs
due to some general toxic effect since H2O2 did not reduce glyceraldehyde-3-phosphate dehydrogenase, alpha-tubulin, beta-fibrinogen,
or albumin mRNA levels, and did not increase lactate dehydrogenase released into the medium. Insulin-mimicked H2O2 action,
reducing the expression of both mRNAs, and N-acetylcysteine, which increases intracellular glutathione levels, completely
reversed the insulin effect on both mRNAs and the H2O2 effect on CYP1A1 mRNA, but only partially reversed the H2O2 effect
on CYP1A2 mRNA. This study indicates that the CYP1A1 and CYP1A2 genes are responsive to oxidative stress and that the majority
of this responsiveness can be modified by cellular redox potential.</abstract><cop>Bethesda, MD</cop><pub>American Society for Biochemistry and Molecular Biology</pub><pmid>8307954</pmid><doi>10.1016/s0021-9258(17)41731-5</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Animals Benzoflavones - pharmacology beta-Naphthoflavone Biological and medical sciences Cell Survival - drug effects Cytochrome P-450 Enzyme System - genetics Cytochrome P-450 Enzyme System - metabolism Fundamental and applied biological sciences. Psychology Gene Expression Genes, jun Glutathione - metabolism Hydrogen Peroxide - pharmacology Insulin - pharmacology Liver - metabolism Male Molecular and cellular biology Molecular genetics Oxidation-Reduction Rats Rats, Sprague-Dawley RNA, Messenger - genetics Stress, Physiological - enzymology Transcription, Genetic - drug effects Transcription. Transcription factor. Splicing. Rna processing |
| title | Down-regulation of P4501A1 and P4501A2 mRNA expression in isolated hepatocytes by oxidative stress |
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