Electrokinetic Evaluation of Individual Exosomes by On-Chip Microcapillary Electrophoresis with Laser Dark-Field Microscopy

Cell-secreted nanovesicles called exosomes are expected as a promising candidate biomarker of various diseases. Toward the future application of exosomes as a disease biomarker for low-invasive diagnostics, challenges remain in the development of sensitive and precise analysis methods for exosomes....

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Veröffentlicht in:Japanese Journal of Applied Physics 2013-06, Vol.52 (6), p.06GK10-06GK10-4
Hauptverfasser: Kato, Kei, Kobayashi, Masashi, Hanamura, Nami, Akagi, Takanori, Kosaka, Nobuyoshi, Ochiya, Takahiro, Ichiki, Takanori
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container_end_page 06GK10-4
container_issue 6
container_start_page 06GK10
container_title Japanese Journal of Applied Physics
container_volume 52
creator Kato, Kei
Kobayashi, Masashi
Hanamura, Nami
Akagi, Takanori
Kosaka, Nobuyoshi
Ochiya, Takahiro
Ichiki, Takanori
description Cell-secreted nanovesicles called exosomes are expected as a promising candidate biomarker of various diseases. Toward the future application of exosomes as a disease biomarker for low-invasive diagnostics, challenges remain in the development of sensitive and precise analysis methods for exosomes. In this study, we performed the electrokinetic evaluation of individual exosomes by the combined use of on-chip microcapillary electrophoresis and laser dark-field microscopy. We extracted exosomes from six types of human cell cultured in a serum-free medium by differential ultracentrifugation and their zeta potential (electrophoretic mobility) were evaluated. We demonstrated that the proposed electrophoresis apparatus is particularly suitable for the tracking analysis of the electrophoretic migration of individual exosomes and enables the accurate evaluation of the zeta potential distribution of exosomes, for the first time. From the experimental results, we found that there is a strong correlation between the average zeta potentials of exosomes and their cells of origin.
doi_str_mv 10.7567/JJAP.52.06GK10
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subjects Biomarkers
Diseases
Electrokinetics
Electrophoresis
Lasers
Microscopy
Migration
Zeta potential
title Electrokinetic Evaluation of Individual Exosomes by On-Chip Microcapillary Electrophoresis with Laser Dark-Field Microscopy
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