Quantitative analysis of low-abundance serological proteins with peptide affinity-based enrichment and pseudo-multiple reaction monitoring by hybrid quadrupole time-of-flight mass spectrometry

[Display omitted] •Development of a workflow for the quantitation of low-abundance serological proteins.•Peptide affinity-based enrichment is effective for the analysis of low-abundance proteins.•Optimization of hybrid Q-TOF MS for pseudo-MRM analysis.•Pseudo-MRM provides an improved signal-to-noise...

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Veröffentlicht in:	Analytica chimica acta 2015-07, Vol.882, p.38-48
Hauptverfasser:	Kim, Kwang Hoe, Ahn, Yeong Hee, Ji, Eun Sun, Lee, Ju Yeon, Kim, Jin Young, An, Hyun Joo, Yoo, Jong Shin
Format:	Artikel
Sprache:	eng
Schlagworte:	Affinity Labels Amino Acid Sequence Blood Proteins - analysis Blood Proteins - chemistry Calibration Humans Hybrid quadrupole time-of-flight mass spectrometer Limit of Detection Low-abundance serological protein Molecular Sequence Data Peptide affinity-based enrichment Peptides - chemistry Pseudo-multiple reaction monitoring Tandem Mass Spectrometry - methods
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