Moderate Exercise Increases Affinity of Large Very Low-Density Lipoproteins for Hydrolysis by Lipoprotein Lipase
Context: Postprandial triglyceride (TG) concentration is independently associated with cardiovascular disease risk. Exercise reduces postprandial TG concentrations, but the mechanisms responsible are unclear. Objective: The objective was to determine the effects of exercise on affinity of chylomicro...
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| Veröffentlicht in: | The journal of clinical endocrinology and metabolism 2015-06, Vol.100 (6), p.2205-2213 |
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| container_title | The journal of clinical endocrinology and metabolism |
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| creator | Ghafouri, Khloud Cooney, Josephine Bedford, Dorothy K Wilson, John Caslake, Muriel J Gill, Jason M. R |
| description | Context:
Postprandial triglyceride (TG) concentration is independently associated with cardiovascular disease risk. Exercise reduces postprandial TG concentrations, but the mechanisms responsible are unclear.
Objective:
The objective was to determine the effects of exercise on affinity of chylomicrons, large very low-density lipoproteins (VLDL1), and smaller VLDL (VLDL2) for lipoprotein lipase (LPL)-mediated TG hydrolysis.
Design:
This was designed as a within-participant crossover study.
Setting:
The setting was a university metabolic investigation unit.
Participants:
Participants were 10 overweight/obese men.
Interventions:
Participants undertook two oral fat tolerance tests, separated by 7–14 days, in which they had blood taken while fasting and for 4 hours after a high-fat mixed meal. On the afternoon before one test, they performed a 90-minute treadmill walk at 50% maximal oxygen uptake (exercise trial [EX]); no exercise was performed before the control trial (CON).
Main Outcome Measures:
We measured circulating TG-rich lipoprotein concentrations and affinity of chylomicrons, VLDL1, and VLDL2 for LPL-mediated TG hydrolysis.
Results:
Exercise significantly reduced fasting VLDL1-TG concentration (CON, 0.49 [0.33–0.72] mmol.L-1; EX, 0.36 [0.22–0.59] mmol.L-1; geometric means [95% confidence interval]; P = .04). Time-averaged postprandial chylomicron-TG (CON, 0.55 ± 0.10 mmol.L-1; EX, 0.39 ± 0.08 mmol.L-1; mean ± SEM; P = .03) and VLDL1-TG (CON, 0.85 ± 0.13 mmol.L-1; EX, 0.66 ± 0.10 mmol.L-1; P = .01) concentrations were both lower in EX than CON. Affinity of VLDL1 for LPL-mediated TG hydrolysis increased by 2.2 (1.3–3.7)-fold [geometric mean (95% confidence interval)] (P = .02) in the fasted state and 2.6 (1.8–2.6)-fold (P = .001) postprandially. Affinity of chylomicrons and VLDL2 was not significantly different between trials.
Conclusions:
Exercise increases affinity of VLDL1 for LPL-mediated TG hydrolysis both fasting and postprandially. This mechanism is likely to contribute to the TG-lowering effect of exercise. |
| doi_str_mv | 10.1210/jc.2015-1196 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1686415827</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1686415827</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4849-83fdb5966d8f6b24b432065e40a221072563b56757d6d55a3278579c474600753</originalsourceid><addsrcrecordid>eNptkE1v1DAQhi0EotvCjTPykQMutuOP5FiV0lYK4gKIm5U4E-olGwdPoiX_HkdbkJDwxZbnmdfjh5BXgl8KKfi7vb-UXGgmRGWekJ2olGZWVPYp2XEuBaus_HZGzhH3nAuldPGcnEldGlsKtSPTx9hBamagN78g-YBA70efoEFAetX3YQzzSmNP6yZ9B_oV0krreGTvYcStUocpTinOEEakfUz0bu1SHFYMSNt_yts5p74gz_pmQHj5uF-QLx9uPl_fsfrT7f31Vc28KlXFyqLvWl0Z05W9aaVqVSG50aB4I_OvrdSmaLWx2nam07oppC21rbyyynBudXFB3pxy8_M_F8DZHQJ6GIZmhLigE6Y0SuhS2oy-PaE-RcQEvZtSODRpdYK7zbHbe7c5dpvjjL9-TF7aA3R_4T9SM6BOwDEOMyT8MSxHSO4BmmF-cDwvlUG2JfI8LGfbVZXbilMbjF30KYwwJUB0-7ikMav6_zS_ARnilyc</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1686415827</pqid></control><display><type>article</type><title>Moderate Exercise Increases Affinity of Large Very Low-Density Lipoproteins for Hydrolysis by Lipoprotein Lipase</title><source>MEDLINE</source><source>Alma/SFX Local Collection</source><source>EZB Electronic Journals Library</source><source>Oxford Journals</source><source>Journals@Ovid Complete</source><creator>Ghafouri, Khloud ; Cooney, Josephine ; Bedford, Dorothy K ; Wilson, John ; Caslake, Muriel J ; Gill, Jason M. R</creator><creatorcontrib>Ghafouri, Khloud ; Cooney, Josephine ; Bedford, Dorothy K ; Wilson, John ; Caslake, Muriel J ; Gill, Jason M. R</creatorcontrib><description>Context:
Postprandial triglyceride (TG) concentration is independently associated with cardiovascular disease risk. Exercise reduces postprandial TG concentrations, but the mechanisms responsible are unclear.
Objective:
The objective was to determine the effects of exercise on affinity of chylomicrons, large very low-density lipoproteins (VLDL1), and smaller VLDL (VLDL2) for lipoprotein lipase (LPL)-mediated TG hydrolysis.
Design:
This was designed as a within-participant crossover study.
Setting:
The setting was a university metabolic investigation unit.
Participants:
Participants were 10 overweight/obese men.
Interventions:
Participants undertook two oral fat tolerance tests, separated by 7–14 days, in which they had blood taken while fasting and for 4 hours after a high-fat mixed meal. On the afternoon before one test, they performed a 90-minute treadmill walk at 50% maximal oxygen uptake (exercise trial [EX]); no exercise was performed before the control trial (CON).
Main Outcome Measures:
We measured circulating TG-rich lipoprotein concentrations and affinity of chylomicrons, VLDL1, and VLDL2 for LPL-mediated TG hydrolysis.
Results:
Exercise significantly reduced fasting VLDL1-TG concentration (CON, 0.49 [0.33–0.72] mmol.L-1; EX, 0.36 [0.22–0.59] mmol.L-1; geometric means [95% confidence interval]; P = .04). Time-averaged postprandial chylomicron-TG (CON, 0.55 ± 0.10 mmol.L-1; EX, 0.39 ± 0.08 mmol.L-1; mean ± SEM; P = .03) and VLDL1-TG (CON, 0.85 ± 0.13 mmol.L-1; EX, 0.66 ± 0.10 mmol.L-1; P = .01) concentrations were both lower in EX than CON. Affinity of VLDL1 for LPL-mediated TG hydrolysis increased by 2.2 (1.3–3.7)-fold [geometric mean (95% confidence interval)] (P = .02) in the fasted state and 2.6 (1.8–2.6)-fold (P = .001) postprandially. Affinity of chylomicrons and VLDL2 was not significantly different between trials.
Conclusions:
Exercise increases affinity of VLDL1 for LPL-mediated TG hydrolysis both fasting and postprandially. This mechanism is likely to contribute to the TG-lowering effect of exercise.</description><identifier>ISSN: 0021-972X</identifier><identifier>EISSN: 1945-7197</identifier><identifier>DOI: 10.1210/jc.2015-1196</identifier><identifier>PMID: 25867814</identifier><language>eng</language><publisher>United States: Endocrine Society</publisher><subject>Adult ; Cross-Over Studies ; Exercise - physiology ; Exercise Test ; Humans ; Hydrolysis ; Lipid Metabolism - physiology ; Lipoprotein Lipase - metabolism ; Lipoproteins, VLDL - metabolism ; Male ; Middle Aged ; Obesity - metabolism ; Overweight - metabolism ; Substrate Specificity ; Young Adult</subject><ispartof>The journal of clinical endocrinology and metabolism, 2015-06, Vol.100 (6), p.2205-2213</ispartof><rights>Copyright © 2015 by the Endocrine Society</rights><rights>Copyright © 2015 by The Endocrine Society</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4849-83fdb5966d8f6b24b432065e40a221072563b56757d6d55a3278579c474600753</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25867814$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ghafouri, Khloud</creatorcontrib><creatorcontrib>Cooney, Josephine</creatorcontrib><creatorcontrib>Bedford, Dorothy K</creatorcontrib><creatorcontrib>Wilson, John</creatorcontrib><creatorcontrib>Caslake, Muriel J</creatorcontrib><creatorcontrib>Gill, Jason M. R</creatorcontrib><title>Moderate Exercise Increases Affinity of Large Very Low-Density Lipoproteins for Hydrolysis by Lipoprotein Lipase</title><title>The journal of clinical endocrinology and metabolism</title><addtitle>J Clin Endocrinol Metab</addtitle><description>Context:
Postprandial triglyceride (TG) concentration is independently associated with cardiovascular disease risk. Exercise reduces postprandial TG concentrations, but the mechanisms responsible are unclear.
Objective:
The objective was to determine the effects of exercise on affinity of chylomicrons, large very low-density lipoproteins (VLDL1), and smaller VLDL (VLDL2) for lipoprotein lipase (LPL)-mediated TG hydrolysis.
Design:
This was designed as a within-participant crossover study.
Setting:
The setting was a university metabolic investigation unit.
Participants:
Participants were 10 overweight/obese men.
Interventions:
Participants undertook two oral fat tolerance tests, separated by 7–14 days, in which they had blood taken while fasting and for 4 hours after a high-fat mixed meal. On the afternoon before one test, they performed a 90-minute treadmill walk at 50% maximal oxygen uptake (exercise trial [EX]); no exercise was performed before the control trial (CON).
Main Outcome Measures:
We measured circulating TG-rich lipoprotein concentrations and affinity of chylomicrons, VLDL1, and VLDL2 for LPL-mediated TG hydrolysis.
Results:
Exercise significantly reduced fasting VLDL1-TG concentration (CON, 0.49 [0.33–0.72] mmol.L-1; EX, 0.36 [0.22–0.59] mmol.L-1; geometric means [95% confidence interval]; P = .04). Time-averaged postprandial chylomicron-TG (CON, 0.55 ± 0.10 mmol.L-1; EX, 0.39 ± 0.08 mmol.L-1; mean ± SEM; P = .03) and VLDL1-TG (CON, 0.85 ± 0.13 mmol.L-1; EX, 0.66 ± 0.10 mmol.L-1; P = .01) concentrations were both lower in EX than CON. Affinity of VLDL1 for LPL-mediated TG hydrolysis increased by 2.2 (1.3–3.7)-fold [geometric mean (95% confidence interval)] (P = .02) in the fasted state and 2.6 (1.8–2.6)-fold (P = .001) postprandially. Affinity of chylomicrons and VLDL2 was not significantly different between trials.
Conclusions:
Exercise increases affinity of VLDL1 for LPL-mediated TG hydrolysis both fasting and postprandially. This mechanism is likely to contribute to the TG-lowering effect of exercise.</description><subject>Adult</subject><subject>Cross-Over Studies</subject><subject>Exercise - physiology</subject><subject>Exercise Test</subject><subject>Humans</subject><subject>Hydrolysis</subject><subject>Lipid Metabolism - physiology</subject><subject>Lipoprotein Lipase - metabolism</subject><subject>Lipoproteins, VLDL - metabolism</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Obesity - metabolism</subject><subject>Overweight - metabolism</subject><subject>Substrate Specificity</subject><subject>Young Adult</subject><issn>0021-972X</issn><issn>1945-7197</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkE1v1DAQhi0EotvCjTPykQMutuOP5FiV0lYK4gKIm5U4E-olGwdPoiX_HkdbkJDwxZbnmdfjh5BXgl8KKfi7vb-UXGgmRGWekJ2olGZWVPYp2XEuBaus_HZGzhH3nAuldPGcnEldGlsKtSPTx9hBamagN78g-YBA70efoEFAetX3YQzzSmNP6yZ9B_oV0krreGTvYcStUocpTinOEEakfUz0bu1SHFYMSNt_yts5p74gz_pmQHj5uF-QLx9uPl_fsfrT7f31Vc28KlXFyqLvWl0Z05W9aaVqVSG50aB4I_OvrdSmaLWx2nam07oppC21rbyyynBudXFB3pxy8_M_F8DZHQJ6GIZmhLigE6Y0SuhS2oy-PaE-RcQEvZtSODRpdYK7zbHbe7c5dpvjjL9-TF7aA3R_4T9SM6BOwDEOMyT8MSxHSO4BmmF-cDwvlUG2JfI8LGfbVZXbilMbjF30KYwwJUB0-7ikMav6_zS_ARnilyc</recordid><startdate>201506</startdate><enddate>201506</enddate><creator>Ghafouri, Khloud</creator><creator>Cooney, Josephine</creator><creator>Bedford, Dorothy K</creator><creator>Wilson, John</creator><creator>Caslake, Muriel J</creator><creator>Gill, Jason M. R</creator><general>Endocrine Society</general><general>Copyright by The Endocrine Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201506</creationdate><title>Moderate Exercise Increases Affinity of Large Very Low-Density Lipoproteins for Hydrolysis by Lipoprotein Lipase</title><author>Ghafouri, Khloud ; Cooney, Josephine ; Bedford, Dorothy K ; Wilson, John ; Caslake, Muriel J ; Gill, Jason M. R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4849-83fdb5966d8f6b24b432065e40a221072563b56757d6d55a3278579c474600753</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Adult</topic><topic>Cross-Over Studies</topic><topic>Exercise - physiology</topic><topic>Exercise Test</topic><topic>Humans</topic><topic>Hydrolysis</topic><topic>Lipid Metabolism - physiology</topic><topic>Lipoprotein Lipase - metabolism</topic><topic>Lipoproteins, VLDL - metabolism</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Obesity - metabolism</topic><topic>Overweight - metabolism</topic><topic>Substrate Specificity</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ghafouri, Khloud</creatorcontrib><creatorcontrib>Cooney, Josephine</creatorcontrib><creatorcontrib>Bedford, Dorothy K</creatorcontrib><creatorcontrib>Wilson, John</creatorcontrib><creatorcontrib>Caslake, Muriel J</creatorcontrib><creatorcontrib>Gill, Jason M. R</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The journal of clinical endocrinology and metabolism</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ghafouri, Khloud</au><au>Cooney, Josephine</au><au>Bedford, Dorothy K</au><au>Wilson, John</au><au>Caslake, Muriel J</au><au>Gill, Jason M. R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Moderate Exercise Increases Affinity of Large Very Low-Density Lipoproteins for Hydrolysis by Lipoprotein Lipase</atitle><jtitle>The journal of clinical endocrinology and metabolism</jtitle><addtitle>J Clin Endocrinol Metab</addtitle><date>2015-06</date><risdate>2015</risdate><volume>100</volume><issue>6</issue><spage>2205</spage><epage>2213</epage><pages>2205-2213</pages><issn>0021-972X</issn><eissn>1945-7197</eissn><abstract>Context:
Postprandial triglyceride (TG) concentration is independently associated with cardiovascular disease risk. Exercise reduces postprandial TG concentrations, but the mechanisms responsible are unclear.
Objective:
The objective was to determine the effects of exercise on affinity of chylomicrons, large very low-density lipoproteins (VLDL1), and smaller VLDL (VLDL2) for lipoprotein lipase (LPL)-mediated TG hydrolysis.
Design:
This was designed as a within-participant crossover study.
Setting:
The setting was a university metabolic investigation unit.
Participants:
Participants were 10 overweight/obese men.
Interventions:
Participants undertook two oral fat tolerance tests, separated by 7–14 days, in which they had blood taken while fasting and for 4 hours after a high-fat mixed meal. On the afternoon before one test, they performed a 90-minute treadmill walk at 50% maximal oxygen uptake (exercise trial [EX]); no exercise was performed before the control trial (CON).
Main Outcome Measures:
We measured circulating TG-rich lipoprotein concentrations and affinity of chylomicrons, VLDL1, and VLDL2 for LPL-mediated TG hydrolysis.
Results:
Exercise significantly reduced fasting VLDL1-TG concentration (CON, 0.49 [0.33–0.72] mmol.L-1; EX, 0.36 [0.22–0.59] mmol.L-1; geometric means [95% confidence interval]; P = .04). Time-averaged postprandial chylomicron-TG (CON, 0.55 ± 0.10 mmol.L-1; EX, 0.39 ± 0.08 mmol.L-1; mean ± SEM; P = .03) and VLDL1-TG (CON, 0.85 ± 0.13 mmol.L-1; EX, 0.66 ± 0.10 mmol.L-1; P = .01) concentrations were both lower in EX than CON. Affinity of VLDL1 for LPL-mediated TG hydrolysis increased by 2.2 (1.3–3.7)-fold [geometric mean (95% confidence interval)] (P = .02) in the fasted state and 2.6 (1.8–2.6)-fold (P = .001) postprandially. Affinity of chylomicrons and VLDL2 was not significantly different between trials.
Conclusions:
Exercise increases affinity of VLDL1 for LPL-mediated TG hydrolysis both fasting and postprandially. This mechanism is likely to contribute to the TG-lowering effect of exercise.</abstract><cop>United States</cop><pub>Endocrine Society</pub><pmid>25867814</pmid><doi>10.1210/jc.2015-1196</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0021-972X |
| ispartof | The journal of clinical endocrinology and metabolism, 2015-06, Vol.100 (6), p.2205-2213 |
| issn | 0021-972X 1945-7197 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1686415827 |
| source | MEDLINE; Alma/SFX Local Collection; EZB Electronic Journals Library; Oxford Journals; Journals@Ovid Complete |
| subjects | Adult Cross-Over Studies Exercise - physiology Exercise Test Humans Hydrolysis Lipid Metabolism - physiology Lipoprotein Lipase - metabolism Lipoproteins, VLDL - metabolism Male Middle Aged Obesity - metabolism Overweight - metabolism Substrate Specificity Young Adult |
| title | Moderate Exercise Increases Affinity of Large Very Low-Density Lipoproteins for Hydrolysis by Lipoprotein Lipase |
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