microRNA expression in autonomous thyroid adenomas: Correlation with mRNA regulation
•Deregulated miRNA were identified in thyroid autonomous adenoma.•Correlations were performed between miRNA and mRNA regulation in the same tumors.•Deregulated miRNA are mostly involved in extracellular matrix organization.•Several miRNA are involved in the negative feedback of the cAMP pathway. The...
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| Veröffentlicht in: | Molecular and cellular endocrinology 2015-08, Vol.411, p.1-10 |
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| creator | Floor, Sébastien L. Trésallet, Christophe Hébrant, Aline Desbuleux, Alice Libert, Frédérick Hoang, Catherine Capello, Matteo Andry, Guy van Staveren, Wilma C.G. Maenhaut, Carine |
| description | •Deregulated miRNA were identified in thyroid autonomous adenoma.•Correlations were performed between miRNA and mRNA regulation in the same tumors.•Deregulated miRNA are mostly involved in extracellular matrix organization.•Several miRNA are involved in the negative feedback of the cAMP pathway.
The objective of the study was to identify the deregulated miRNA in autonomous adenoma and to correlate the data with mRNA regulation. Seven autonomous adenoma with adjacent healthy thyroid tissues were investigated. Twelve miRNAs were downregulated and one was upregulated in the tumors. Combining bioinformatic mRNA target prediction and microarray data on mRNA regulations allowed to identify mRNA targets of our deregulated miRNAs. A large enrichment in mRNA encoding proteins involved in extracellular matrix organization and different phosphodiesterases were identified among these putative targets. The direct interaction between miR-101-3p and miR-144-3p and PDE4D mRNA was experimentally validated. The global miRNA profiles were not greatly modified, confirming the definition of these tumors as minimal deviation tumors. These results support a role for miRNA in the regulation of extracellular matrix proteins and tissue remodeling occurring during tumor development, and in the important negative feedback of the cAMP pathway, which limits the consequences of its constitutive activation in these tumors. |
| doi_str_mv | 10.1016/j.mce.2015.04.001 |
| format | Article |
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The objective of the study was to identify the deregulated miRNA in autonomous adenoma and to correlate the data with mRNA regulation. Seven autonomous adenoma with adjacent healthy thyroid tissues were investigated. Twelve miRNAs were downregulated and one was upregulated in the tumors. Combining bioinformatic mRNA target prediction and microarray data on mRNA regulations allowed to identify mRNA targets of our deregulated miRNAs. A large enrichment in mRNA encoding proteins involved in extracellular matrix organization and different phosphodiesterases were identified among these putative targets. The direct interaction between miR-101-3p and miR-144-3p and PDE4D mRNA was experimentally validated. The global miRNA profiles were not greatly modified, confirming the definition of these tumors as minimal deviation tumors. These results support a role for miRNA in the regulation of extracellular matrix proteins and tissue remodeling occurring during tumor development, and in the important negative feedback of the cAMP pathway, which limits the consequences of its constitutive activation in these tumors.</description><identifier>ISSN: 0303-7207</identifier><identifier>EISSN: 1872-8057</identifier><identifier>DOI: 10.1016/j.mce.2015.04.001</identifier><identifier>PMID: 25916957</identifier><language>eng</language><publisher>Ireland: Elsevier Ireland Ltd</publisher><subject>Adenoma - genetics ; Adenoma - metabolism ; Adenoma - pathology ; Adult ; Aged ; Aged, 80 and over ; Autonomous adenoma ; cAMP ; Cyclic Nucleotide Phosphodiesterases, Type 4 - genetics ; Cyclic Nucleotide Phosphodiesterases, Type 4 - metabolism ; Down-Regulation ; ECM ; Female ; Gene Expression Profiling ; Gene Expression Regulation, Neoplastic ; Humans ; Male ; MicroRNAs - genetics ; MicroRNAs - metabolism ; Middle Aged ; miRNA ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Signal Transduction - genetics ; Thyroid Neoplasms - genetics ; Thyroid Neoplasms - metabolism ; Thyroid Neoplasms - pathology ; Tissue Array Analysis</subject><ispartof>Molecular and cellular endocrinology, 2015-08, Vol.411, p.1-10</ispartof><rights>2015 Elsevier Ireland Ltd</rights><rights>Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c423t-2bd3f7c4fa425b3fdbe61b11aa26aded909944b14af3fd02800bcccfc40f3b5d3</citedby><cites>FETCH-LOGICAL-c423t-2bd3f7c4fa425b3fdbe61b11aa26aded909944b14af3fd02800bcccfc40f3b5d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.mce.2015.04.001$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,27929,27930,46000</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25916957$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Floor, Sébastien L.</creatorcontrib><creatorcontrib>Trésallet, Christophe</creatorcontrib><creatorcontrib>Hébrant, Aline</creatorcontrib><creatorcontrib>Desbuleux, Alice</creatorcontrib><creatorcontrib>Libert, Frédérick</creatorcontrib><creatorcontrib>Hoang, Catherine</creatorcontrib><creatorcontrib>Capello, Matteo</creatorcontrib><creatorcontrib>Andry, Guy</creatorcontrib><creatorcontrib>van Staveren, Wilma C.G.</creatorcontrib><creatorcontrib>Maenhaut, Carine</creatorcontrib><title>microRNA expression in autonomous thyroid adenomas: Correlation with mRNA regulation</title><title>Molecular and cellular endocrinology</title><addtitle>Mol Cell Endocrinol</addtitle><description>•Deregulated miRNA were identified in thyroid autonomous adenoma.•Correlations were performed between miRNA and mRNA regulation in the same tumors.•Deregulated miRNA are mostly involved in extracellular matrix organization.•Several miRNA are involved in the negative feedback of the cAMP pathway.
The objective of the study was to identify the deregulated miRNA in autonomous adenoma and to correlate the data with mRNA regulation. Seven autonomous adenoma with adjacent healthy thyroid tissues were investigated. Twelve miRNAs were downregulated and one was upregulated in the tumors. Combining bioinformatic mRNA target prediction and microarray data on mRNA regulations allowed to identify mRNA targets of our deregulated miRNAs. A large enrichment in mRNA encoding proteins involved in extracellular matrix organization and different phosphodiesterases were identified among these putative targets. The direct interaction between miR-101-3p and miR-144-3p and PDE4D mRNA was experimentally validated. The global miRNA profiles were not greatly modified, confirming the definition of these tumors as minimal deviation tumors. These results support a role for miRNA in the regulation of extracellular matrix proteins and tissue remodeling occurring during tumor development, and in the important negative feedback of the cAMP pathway, which limits the consequences of its constitutive activation in these tumors.</description><subject>Adenoma - genetics</subject><subject>Adenoma - metabolism</subject><subject>Adenoma - pathology</subject><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Autonomous adenoma</subject><subject>cAMP</subject><subject>Cyclic Nucleotide Phosphodiesterases, Type 4 - genetics</subject><subject>Cyclic Nucleotide Phosphodiesterases, Type 4 - metabolism</subject><subject>Down-Regulation</subject><subject>ECM</subject><subject>Female</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Humans</subject><subject>Male</subject><subject>MicroRNAs - genetics</subject><subject>MicroRNAs - metabolism</subject><subject>Middle Aged</subject><subject>miRNA</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Signal Transduction - genetics</subject><subject>Thyroid Neoplasms - genetics</subject><subject>Thyroid Neoplasms - metabolism</subject><subject>Thyroid Neoplasms - pathology</subject><subject>Tissue Array Analysis</subject><issn>0303-7207</issn><issn>1872-8057</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMtOwzAQRS0EoqXwAWxQlmwSxo7zglVV8ZIQSKisLceeUFdNXOwE6N_jqoUlK0vjM3dmDiHnFBIKNL9aJq3ChAHNEuAJAD0gY1oWLC4hKw7JGFJI44JBMSIn3i8BoMhYeUxGLKtoXmXFmMxbo5x9fZ5G-L126L2xXWS6SA697WxrBx_1i42zRkdSY6hIfx3NrHO4kv2W_TL9Imq3AQ7fh13xlBw1cuXxbP9OyNvd7Xz2ED-93D_Opk-x4iztY1brtCkUbyRnWZ02usac1pRKyfIwTFdQVZzXlMsmfAIrAWqlVKM4NGmd6XRCLne5a2c_BvS9aI1XuFrJDsPmguZlzilNcx5QukPDtd47bMTamVa6jaAgtjLFUgSZYitTABdBZui52McPdYv6r-PXXgBudgCGIz8NOuGVwU6hNg5VL7Q1_8T_ANKWhpE</recordid><startdate>20150815</startdate><enddate>20150815</enddate><creator>Floor, Sébastien L.</creator><creator>Trésallet, Christophe</creator><creator>Hébrant, Aline</creator><creator>Desbuleux, Alice</creator><creator>Libert, Frédérick</creator><creator>Hoang, Catherine</creator><creator>Capello, Matteo</creator><creator>Andry, Guy</creator><creator>van Staveren, Wilma C.G.</creator><creator>Maenhaut, Carine</creator><general>Elsevier Ireland Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20150815</creationdate><title>microRNA expression in autonomous thyroid adenomas: Correlation with mRNA regulation</title><author>Floor, Sébastien L. ; Trésallet, Christophe ; Hébrant, Aline ; Desbuleux, Alice ; Libert, Frédérick ; Hoang, Catherine ; Capello, Matteo ; Andry, Guy ; van Staveren, Wilma C.G. ; Maenhaut, Carine</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c423t-2bd3f7c4fa425b3fdbe61b11aa26aded909944b14af3fd02800bcccfc40f3b5d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Adenoma - genetics</topic><topic>Adenoma - metabolism</topic><topic>Adenoma - pathology</topic><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Autonomous adenoma</topic><topic>cAMP</topic><topic>Cyclic Nucleotide Phosphodiesterases, Type 4 - genetics</topic><topic>Cyclic Nucleotide Phosphodiesterases, Type 4 - metabolism</topic><topic>Down-Regulation</topic><topic>ECM</topic><topic>Female</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Humans</topic><topic>Male</topic><topic>MicroRNAs - genetics</topic><topic>MicroRNAs - metabolism</topic><topic>Middle Aged</topic><topic>miRNA</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Signal Transduction - genetics</topic><topic>Thyroid Neoplasms - genetics</topic><topic>Thyroid Neoplasms - metabolism</topic><topic>Thyroid Neoplasms - pathology</topic><topic>Tissue Array Analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Floor, Sébastien L.</creatorcontrib><creatorcontrib>Trésallet, Christophe</creatorcontrib><creatorcontrib>Hébrant, Aline</creatorcontrib><creatorcontrib>Desbuleux, Alice</creatorcontrib><creatorcontrib>Libert, Frédérick</creatorcontrib><creatorcontrib>Hoang, Catherine</creatorcontrib><creatorcontrib>Capello, Matteo</creatorcontrib><creatorcontrib>Andry, Guy</creatorcontrib><creatorcontrib>van Staveren, Wilma C.G.</creatorcontrib><creatorcontrib>Maenhaut, Carine</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular and cellular endocrinology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Floor, Sébastien L.</au><au>Trésallet, Christophe</au><au>Hébrant, Aline</au><au>Desbuleux, Alice</au><au>Libert, Frédérick</au><au>Hoang, Catherine</au><au>Capello, Matteo</au><au>Andry, Guy</au><au>van Staveren, Wilma C.G.</au><au>Maenhaut, Carine</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>microRNA expression in autonomous thyroid adenomas: Correlation with mRNA regulation</atitle><jtitle>Molecular and cellular endocrinology</jtitle><addtitle>Mol Cell Endocrinol</addtitle><date>2015-08-15</date><risdate>2015</risdate><volume>411</volume><spage>1</spage><epage>10</epage><pages>1-10</pages><issn>0303-7207</issn><eissn>1872-8057</eissn><abstract>•Deregulated miRNA were identified in thyroid autonomous adenoma.•Correlations were performed between miRNA and mRNA regulation in the same tumors.•Deregulated miRNA are mostly involved in extracellular matrix organization.•Several miRNA are involved in the negative feedback of the cAMP pathway.
The objective of the study was to identify the deregulated miRNA in autonomous adenoma and to correlate the data with mRNA regulation. Seven autonomous adenoma with adjacent healthy thyroid tissues were investigated. Twelve miRNAs were downregulated and one was upregulated in the tumors. Combining bioinformatic mRNA target prediction and microarray data on mRNA regulations allowed to identify mRNA targets of our deregulated miRNAs. A large enrichment in mRNA encoding proteins involved in extracellular matrix organization and different phosphodiesterases were identified among these putative targets. The direct interaction between miR-101-3p and miR-144-3p and PDE4D mRNA was experimentally validated. The global miRNA profiles were not greatly modified, confirming the definition of these tumors as minimal deviation tumors. These results support a role for miRNA in the regulation of extracellular matrix proteins and tissue remodeling occurring during tumor development, and in the important negative feedback of the cAMP pathway, which limits the consequences of its constitutive activation in these tumors.</abstract><cop>Ireland</cop><pub>Elsevier Ireland Ltd</pub><pmid>25916957</pmid><doi>10.1016/j.mce.2015.04.001</doi><tpages>10</tpages></addata></record> |
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| subjects | Adenoma - genetics Adenoma - metabolism Adenoma - pathology Adult Aged Aged, 80 and over Autonomous adenoma cAMP Cyclic Nucleotide Phosphodiesterases, Type 4 - genetics Cyclic Nucleotide Phosphodiesterases, Type 4 - metabolism Down-Regulation ECM Female Gene Expression Profiling Gene Expression Regulation, Neoplastic Humans Male MicroRNAs - genetics MicroRNAs - metabolism Middle Aged miRNA RNA, Messenger - genetics RNA, Messenger - metabolism Signal Transduction - genetics Thyroid Neoplasms - genetics Thyroid Neoplasms - metabolism Thyroid Neoplasms - pathology Tissue Array Analysis |
| title | microRNA expression in autonomous thyroid adenomas: Correlation with mRNA regulation |
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