Precise quantitation of 136 urinary proteins by LC/MRM-MS using stable isotope labeled peptides as internal standards for biomarker discovery and/or verification studies

[Display omitted] •Most multiplexed and broadest protein panel quantified via MRM with SIS peptides.•The 136 quantified urinary proteins span >5 orders of magnitude in concentration.•Quantitative reproducibility of 8.6% CV, on average, from a process triplicate.•Largest panel to date can serve as...

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Veröffentlicht in:	Methods (San Diego, Calif.) Calif.), 2015-06, Vol.81, p.24-33
Hauptverfasser:	Percy, Andrew J., Yang, Juncong, Hardie, Darryl B., Chambers, Andrew G., Tamura-Wells, Jessica, Borchers, Christoph H.
Format:	Artikel
Sprache:	eng
Schlagworte:	Aged Biomarkers, Tumor - urine Carbon Radioisotopes Chromatography, Liquid - methods Chromatography, Liquid - standards Humans Internal standard Male Mass Spectrometry - methods Mass Spectrometry - standards MRM Multiplexed Neoplasm Proteins - urine Nitrogen Radioisotopes Peptide Peptides - standards Prostatic Neoplasms - diagnosis Protein quantitation Proteomics - methods Reference Standards Urinalysis - methods Urine
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