Isolation of adipose-derived stem cells: a comparison among different methods

Adipose-derived stromal cells (ASCs) are usually isolated by digestion with collagenase. We have compared alternative methods to isolate ASCs in a more economically viable protocol. Nine protocols using red blood cells lysis buffer solution, trypsin, collagenase and centrifugation were compared; the...

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Veröffentlicht in:	Biotechnology letters 2014-04, Vol.36 (4), p.693-702
Hauptverfasser:	Markarian, Carolina Franke, Frey, Gianna Zaffari, Silveira, Maiele Dornelles, Chem, Eduardo Mainieri, Milani, Adriana Rosa, Ely, Pedro Bins, Horn, Ana Paula, Nardi, Nance Beyer, Camassola, Melissa
Format:	Artikel
Sprache:	eng
Schlagworte:	Adipocytes Adipose Tissue - cytology Applied Microbiology Biochemistry Biocompatibility Biomedical and Life Sciences Biomedical materials Biotechnology bone formation Bones Cell Proliferation Cell Separation - methods Cell Survival cell viability Centrifugation Collagen Comparative studies Differentiation Digestion erythrocytes Immunophenotyping Life Sciences lipogenesis Microbiology Original Research Paper Specimen Handling - methods Stem cells Stem Cells - physiology stromal cells tissue engineering Trypsin
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