Automated and portable solid phase extraction platform for immuno-detection of 17β-estradiol in water
•A fully automated and portable platform for pre-concentration of water samples is proposed.•The method is based on solid phase extraction.•500-Fold pre-concentration of 100ml samples at ng/L levels of 17β-estradiol was achieved.•The method is compatible with analysis by an immunoassay.•The system i...
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| Veröffentlicht in: | Journal of Chromatography A 2015-02, Vol.1381, p.22-28 |
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| container_title | Journal of Chromatography A |
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| creator | Heub, Sarah Tscharner, Noe Monnier, Véronique Kehl, Florian Dittrich, Petra S. Follonier, Stéphane Barbe, Laurent |
| description | •A fully automated and portable platform for pre-concentration of water samples is proposed.•The method is based on solid phase extraction.•500-Fold pre-concentration of 100ml samples at ng/L levels of 17β-estradiol was achieved.•The method is compatible with analysis by an immunoassay.•The system is suitable for at-site analysis and monitoring applications.
A fully automated and portable system for solid phase extraction (SPE) has been developed for the analysis of the natural hormone 17β-estradiol (E2) in environmental water by enzyme linked immuno-sorbent assay (ELISA). The system has been validated with de-ionized and artificial sea water as model samples and allowed for pre-concentration of E2 at levels of 1, 10 and 100ng/L with only 100ml of sample. Recoveries ranged from 24±3% to 107±6% depending on the concentration and sample matrix. The method successfully allowed us to determine the concentration of two seawater samples. A concentration of 15.1±0.3ng/L of E2 was measured in a sample obtained from a food production process, and 8.8±0.7ng/L in a sample from the Adriatic Sea. The system would be suitable for continuous monitoring of water quality as it is user friendly, and as the method is reproducible and totally compatible with the analysis of water sample by simple immunoassays and other detection methods such as biosensors. |
| doi_str_mv | 10.1016/j.chroma.2014.12.076 |
| format | Article |
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A fully automated and portable system for solid phase extraction (SPE) has been developed for the analysis of the natural hormone 17β-estradiol (E2) in environmental water by enzyme linked immuno-sorbent assay (ELISA). The system has been validated with de-ionized and artificial sea water as model samples and allowed for pre-concentration of E2 at levels of 1, 10 and 100ng/L with only 100ml of sample. Recoveries ranged from 24±3% to 107±6% depending on the concentration and sample matrix. The method successfully allowed us to determine the concentration of two seawater samples. A concentration of 15.1±0.3ng/L of E2 was measured in a sample obtained from a food production process, and 8.8±0.7ng/L in a sample from the Adriatic Sea. The system would be suitable for continuous monitoring of water quality as it is user friendly, and as the method is reproducible and totally compatible with the analysis of water sample by simple immunoassays and other detection methods such as biosensors.</description><identifier>ISSN: 0021-9673</identifier><identifier>EISSN: 1873-3778</identifier><identifier>DOI: 10.1016/j.chroma.2014.12.076</identifier><identifier>PMID: 25604269</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>17β-Estradiol ; Automated ; Biosensors ; Enzyme-linked immuno-sorbent assay ; Enzymes ; Estradiol - analysis ; Estrogens - analysis ; Extraction ; Immunoassay ; Portability ; Sample preparation ; Sea water ; Seawater - analysis ; Solid phase extraction ; Solid Phase Extraction - methods ; Solid phases ; Water - analysis ; Water Pollutants, Chemical - analysis ; Water quality</subject><ispartof>Journal of Chromatography A, 2015-02, Vol.1381, p.22-28</ispartof><rights>2015 Elsevier B.V.</rights><rights>Copyright © 2015 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c498t-2675e20a069616cc044a53df3be5b247c501a28b250b855f6bc42d468bc2c8d53</citedby><cites>FETCH-LOGICAL-c498t-2675e20a069616cc044a53df3be5b247c501a28b250b855f6bc42d468bc2c8d53</cites><orcidid>0000-0002-3242-8103</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.chroma.2014.12.076$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25604269$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Heub, Sarah</creatorcontrib><creatorcontrib>Tscharner, Noe</creatorcontrib><creatorcontrib>Monnier, Véronique</creatorcontrib><creatorcontrib>Kehl, Florian</creatorcontrib><creatorcontrib>Dittrich, Petra S.</creatorcontrib><creatorcontrib>Follonier, Stéphane</creatorcontrib><creatorcontrib>Barbe, Laurent</creatorcontrib><title>Automated and portable solid phase extraction platform for immuno-detection of 17β-estradiol in water</title><title>Journal of Chromatography A</title><addtitle>J Chromatogr A</addtitle><description>•A fully automated and portable platform for pre-concentration of water samples is proposed.•The method is based on solid phase extraction.•500-Fold pre-concentration of 100ml samples at ng/L levels of 17β-estradiol was achieved.•The method is compatible with analysis by an immunoassay.•The system is suitable for at-site analysis and monitoring applications.
A fully automated and portable system for solid phase extraction (SPE) has been developed for the analysis of the natural hormone 17β-estradiol (E2) in environmental water by enzyme linked immuno-sorbent assay (ELISA). The system has been validated with de-ionized and artificial sea water as model samples and allowed for pre-concentration of E2 at levels of 1, 10 and 100ng/L with only 100ml of sample. Recoveries ranged from 24±3% to 107±6% depending on the concentration and sample matrix. The method successfully allowed us to determine the concentration of two seawater samples. A concentration of 15.1±0.3ng/L of E2 was measured in a sample obtained from a food production process, and 8.8±0.7ng/L in a sample from the Adriatic Sea. The system would be suitable for continuous monitoring of water quality as it is user friendly, and as the method is reproducible and totally compatible with the analysis of water sample by simple immunoassays and other detection methods such as biosensors.</description><subject>17β-Estradiol</subject><subject>Automated</subject><subject>Biosensors</subject><subject>Enzyme-linked immuno-sorbent assay</subject><subject>Enzymes</subject><subject>Estradiol - analysis</subject><subject>Estrogens - analysis</subject><subject>Extraction</subject><subject>Immunoassay</subject><subject>Portability</subject><subject>Sample preparation</subject><subject>Sea water</subject><subject>Seawater - analysis</subject><subject>Solid phase extraction</subject><subject>Solid Phase Extraction - methods</subject><subject>Solid phases</subject><subject>Water - analysis</subject><subject>Water Pollutants, Chemical - analysis</subject><subject>Water quality</subject><issn>0021-9673</issn><issn>1873-3778</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1q3TAQhUVpaW7TvkEpWnZjdyTr724CIfQPAt20ayFLY6KLbd1IdpK-Vh-kz1QFJ1mGbiSEvnNmOIeQ9wxaBkx9OrT-KqfJtRyYaBlvQasXZMeM7ppOa_OS7AA4a_ZKdyfkTSkHAKZB89fkhEsFgqv9jgzn61JNFgzUzYEeU15cPyItaYz1eeUKUrxbsvNLTDM9jm4ZUp5oPWicpnVOTcAFt980UKb__mmwVEGIaaRxprfVPL8lrwY3Fnz3cJ-SX18-_7z41lz--Pr94vyy8WJvloYrLZGDA7VXTHkPQjjZhaHrUfZcaC-BOW56LqE3Ug6q94IHoUzvuTdBdqfk4-Z7zOl6rXvYKRaP4-hmTGuxTBlpOANu_gPVXWeUNKKiYkN9TqVkHOwxx8nl35aBvS_DHuxWhr0vwzJuaxlV9uFhwtpPGJ5Ej-lX4GwDsEZyEzHb4iPOHkPMNVIbUnx-wj9gsJ2e</recordid><startdate>20150213</startdate><enddate>20150213</enddate><creator>Heub, Sarah</creator><creator>Tscharner, Noe</creator><creator>Monnier, Véronique</creator><creator>Kehl, Florian</creator><creator>Dittrich, Petra S.</creator><creator>Follonier, Stéphane</creator><creator>Barbe, Laurent</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QH</scope><scope>7TV</scope><scope>7UA</scope><scope>C1K</scope><scope>F1W</scope><scope>H97</scope><scope>L.G</scope><scope>7U5</scope><scope>8FD</scope><scope>L7M</scope><orcidid>https://orcid.org/0000-0002-3242-8103</orcidid></search><sort><creationdate>20150213</creationdate><title>Automated and portable solid phase extraction platform for immuno-detection of 17β-estradiol in water</title><author>Heub, Sarah ; Tscharner, Noe ; Monnier, Véronique ; Kehl, Florian ; Dittrich, Petra S. ; Follonier, Stéphane ; Barbe, Laurent</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c498t-2675e20a069616cc044a53df3be5b247c501a28b250b855f6bc42d468bc2c8d53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>17β-Estradiol</topic><topic>Automated</topic><topic>Biosensors</topic><topic>Enzyme-linked immuno-sorbent assay</topic><topic>Enzymes</topic><topic>Estradiol - analysis</topic><topic>Estrogens - analysis</topic><topic>Extraction</topic><topic>Immunoassay</topic><topic>Portability</topic><topic>Sample preparation</topic><topic>Sea water</topic><topic>Seawater - analysis</topic><topic>Solid phase extraction</topic><topic>Solid Phase Extraction - methods</topic><topic>Solid phases</topic><topic>Water - analysis</topic><topic>Water Pollutants, Chemical - analysis</topic><topic>Water quality</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Heub, Sarah</creatorcontrib><creatorcontrib>Tscharner, Noe</creatorcontrib><creatorcontrib>Monnier, Véronique</creatorcontrib><creatorcontrib>Kehl, Florian</creatorcontrib><creatorcontrib>Dittrich, Petra S.</creatorcontrib><creatorcontrib>Follonier, Stéphane</creatorcontrib><creatorcontrib>Barbe, Laurent</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aqualine</collection><collection>Pollution Abstracts</collection><collection>Water Resources Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Journal of Chromatography A</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Heub, Sarah</au><au>Tscharner, Noe</au><au>Monnier, Véronique</au><au>Kehl, Florian</au><au>Dittrich, Petra S.</au><au>Follonier, Stéphane</au><au>Barbe, Laurent</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Automated and portable solid phase extraction platform for immuno-detection of 17β-estradiol in water</atitle><jtitle>Journal of Chromatography A</jtitle><addtitle>J Chromatogr A</addtitle><date>2015-02-13</date><risdate>2015</risdate><volume>1381</volume><spage>22</spage><epage>28</epage><pages>22-28</pages><issn>0021-9673</issn><eissn>1873-3778</eissn><abstract>•A fully automated and portable platform for pre-concentration of water samples is proposed.•The method is based on solid phase extraction.•500-Fold pre-concentration of 100ml samples at ng/L levels of 17β-estradiol was achieved.•The method is compatible with analysis by an immunoassay.•The system is suitable for at-site analysis and monitoring applications.
A fully automated and portable system for solid phase extraction (SPE) has been developed for the analysis of the natural hormone 17β-estradiol (E2) in environmental water by enzyme linked immuno-sorbent assay (ELISA). The system has been validated with de-ionized and artificial sea water as model samples and allowed for pre-concentration of E2 at levels of 1, 10 and 100ng/L with only 100ml of sample. Recoveries ranged from 24±3% to 107±6% depending on the concentration and sample matrix. The method successfully allowed us to determine the concentration of two seawater samples. A concentration of 15.1±0.3ng/L of E2 was measured in a sample obtained from a food production process, and 8.8±0.7ng/L in a sample from the Adriatic Sea. The system would be suitable for continuous monitoring of water quality as it is user friendly, and as the method is reproducible and totally compatible with the analysis of water sample by simple immunoassays and other detection methods such as biosensors.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>25604269</pmid><doi>10.1016/j.chroma.2014.12.076</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0002-3242-8103</orcidid></addata></record> |
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| subjects | 17β-Estradiol Automated Biosensors Enzyme-linked immuno-sorbent assay Enzymes Estradiol - analysis Estrogens - analysis Extraction Immunoassay Portability Sample preparation Sea water Seawater - analysis Solid phase extraction Solid Phase Extraction - methods Solid phases Water - analysis Water Pollutants, Chemical - analysis Water quality |
| title | Automated and portable solid phase extraction platform for immuno-detection of 17β-estradiol in water |
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