Substrate specificity engineering of Escherichia coli derived fructosamine 6-kinase

A three-dimensional structural model of Escherichia coli fructosamine 6-kinase (FN6K), an enzyme that phosphorylates fructosamines at C6 and catalyzes the production of the fructosamine 6-phosphate stable intermediate, was generated using the crystal structure of 2-keto-3-deoxygluconate kinase isola...

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Veröffentlicht in:	Biotechnology letters 2013-02, Vol.35 (2), p.253-258
Hauptverfasser:	Kojima, Katsuhiro, Mikami-Sakaguchi, Akane, Kameya, Miho, Miyamoto, Yusuke, Ferri, Stefano, Tsugawa, Wakako, Sode, Koji
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Substitution Amino acids Applied Microbiology Bacillus subtilis Biochemistry Biomedical and Life Sciences Biotechnology Catalytic Domain Crystal structure E coli engineering Enzymes Escherichia coli Escherichia coli - enzymology Fructosamine - metabolism Kinases Life Sciences Lysine - analogs & derivatives Lysine - metabolism Metabolic Engineering Microbiology Models, Molecular Mutagenesis Mutagenesis, Site-Directed Mutant Proteins - genetics Mutant Proteins - metabolism mutants Original Research Paper Phosphotransferases - genetics Phosphotransferases - metabolism Protein Engineering Residues site-directed mutagenesis Substrate Specificity Substrates Thermus thermophilus Three dimensional models Valine - analogs & derivatives Valine - metabolism
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