Effect of acute hyperglycemia on moderately hypothermic GL261 mouse glioma monitored by T1-weighted DCE MRI
Objective We sought to evaluate the effects of acute hyperglycemia induced by intraperitoneal injection of glucose (2.7 g/kg) on vascular delivery to GL261 mouse gliomas kept at moderate hypothermia (~30 °C). Materials and methods Seven GL261 glioma-bearing mice were studied by T1-weighted DCE MRI b...
Gespeichert in:
| Veröffentlicht in: | Magma (New York, N.Y.) N.Y.), 2015-04, Vol.28 (2), p.119-126 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 126 |
|---|---|
| container_issue | 2 |
| container_start_page | 119 |
| container_title | Magma (New York, N.Y.) |
| container_volume | 28 |
| creator | Simões, Rui V. Ortuño, Juan E. Bokacheva, Louisa Candiota, Ana P. Ledesma-Carbayo, Maria J. Delgado-Goñi, Teresa García-Martín, Maria L. Santos, Andrés Arús, Carles |
| description | Objective
We sought to evaluate the effects of acute hyperglycemia induced by intraperitoneal injection of glucose (2.7 g/kg) on vascular delivery to GL261 mouse gliomas kept at moderate hypothermia (~30 °C).
Materials and methods
Seven GL261 glioma-bearing mice were studied by T1-weighted DCE MRI before and after an injection of glucose (
n
= 4) or saline (
n
= 3). Maximum relative contrast enhancement (RCE) and initial area under the enhancement curve (IAUC) were determined in each pixel.
Results
The mean tumor parameter values showed no significant changes after injecting either saline (RCE −5.9 ± 5.0 %; IAUC −3.7 ± 3.6 %) or glucose (RCE −1.6 ± 9.0 %; IAUC +0.6 ± 6.4 %). Pixel-by-pixel analysis revealed small post-injection changes in RCE and IAUC between the glucose and saline groups, all within 13 % range of their baseline values.
Conclusion
Perturbing the metabolism of GL261 tumors kept at moderate hypothermia with hyperglycemia did not induce significant changes in the permeability/perfusion of these tumors. This is relevant for future studies with this model since regional differences in glucose accumulation could thus reflect basal heterogeneities in vasculature and/or metabolism of GL261 tumors. |
| doi_str_mv | 10.1007/s10334-014-0447-2 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1685808580</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1671213548</sourcerecordid><originalsourceid>FETCH-LOGICAL-c502t-7d3dfa98a0ef484d5674a303d8b719971d431921c407bf501c31ccee217f32293</originalsourceid><addsrcrecordid>eNqNkUFv1DAQhS0EokvLD-CCfOQS8NiO7RzRspRKW1VC7dnyOuPdlCRe7ERV_j1ebeGIehhZo_fNk_UeIR-AfQbG9JcMTAhZMSgjpa74K7ICUfPKKAWvyYo1ylQ1l-KCvMv5kTEONRNvyQWXDShp9Ir82oSAfqIxUOfnCelhOWLa94vHoXM0jnSILSY3Yb-ctDgdMA2dp9dbrqCIc0a677s4uLKM3RQTtnS30HuonrDbH6ayfltv6O3PmyvyJrg-4_vn95I8fN_cr39U27vrm_XXbeVrxqdKt6INrjGOYZBGtrXS0gkmWrPT0DQaWimg4eAl07tQM_ACvEfkoIPgvBGX5NPZ95ji7xnzZIcue-x7N2L5rwVlasNO8xK0JNso9RJUAy_pS1NQOKM-xZwTBntM3eDSYoHZU3P23JwtzdlTc5aXm4_P9vNuwPbfxd-qCsDPQC7SuMdkH-OcxhLkf1z_AIOzoSo</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1671213548</pqid></control><display><type>article</type><title>Effect of acute hyperglycemia on moderately hypothermic GL261 mouse glioma monitored by T1-weighted DCE MRI</title><source>MEDLINE</source><source>Springer Nature - Complete Springer Journals</source><creator>Simões, Rui V. ; Ortuño, Juan E. ; Bokacheva, Louisa ; Candiota, Ana P. ; Ledesma-Carbayo, Maria J. ; Delgado-Goñi, Teresa ; García-Martín, Maria L. ; Santos, Andrés ; Arús, Carles</creator><creatorcontrib>Simões, Rui V. ; Ortuño, Juan E. ; Bokacheva, Louisa ; Candiota, Ana P. ; Ledesma-Carbayo, Maria J. ; Delgado-Goñi, Teresa ; García-Martín, Maria L. ; Santos, Andrés ; Arús, Carles</creatorcontrib><description>Objective
We sought to evaluate the effects of acute hyperglycemia induced by intraperitoneal injection of glucose (2.7 g/kg) on vascular delivery to GL261 mouse gliomas kept at moderate hypothermia (~30 °C).
Materials and methods
Seven GL261 glioma-bearing mice were studied by T1-weighted DCE MRI before and after an injection of glucose (
n
= 4) or saline (
n
= 3). Maximum relative contrast enhancement (RCE) and initial area under the enhancement curve (IAUC) were determined in each pixel.
Results
The mean tumor parameter values showed no significant changes after injecting either saline (RCE −5.9 ± 5.0 %; IAUC −3.7 ± 3.6 %) or glucose (RCE −1.6 ± 9.0 %; IAUC +0.6 ± 6.4 %). Pixel-by-pixel analysis revealed small post-injection changes in RCE and IAUC between the glucose and saline groups, all within 13 % range of their baseline values.
Conclusion
Perturbing the metabolism of GL261 tumors kept at moderate hypothermia with hyperglycemia did not induce significant changes in the permeability/perfusion of these tumors. This is relevant for future studies with this model since regional differences in glucose accumulation could thus reflect basal heterogeneities in vasculature and/or metabolism of GL261 tumors.</description><identifier>ISSN: 0968-5243</identifier><identifier>EISSN: 1352-8661</identifier><identifier>DOI: 10.1007/s10334-014-0447-2</identifier><identifier>PMID: 24916487</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Acute Disease ; Animals ; Biology ; Biomedical Engineering and Bioengineering ; Brain Neoplasms - complications ; Brain Neoplasms - pathology ; Brain Neoplasms - therapy ; Cell Line, Tumor ; Computer Appl. in Life Sciences ; Diffusion Magnetic Resonance Imaging - methods ; Female ; Glioma ; Glucose ; Health Informatics ; Heterogeneity ; Hyperglycemia ; Hyperglycemia - complications ; Hyperglycemia - pathology ; Hypothermia ; Hypothermia, Induced - methods ; Imaging ; Medicine & Public Health ; Metabolism ; Mice ; Mice, Inbred C57BL ; Pixels ; Radiology ; Reproducibility of Results ; Research Article ; Saline ; Sensitivity and Specificity ; Solid State Physics ; Treatment Outcome ; Tumor Burden ; Tumors</subject><ispartof>Magma (New York, N.Y.), 2015-04, Vol.28 (2), p.119-126</ispartof><rights>ESMRMB 2014</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c502t-7d3dfa98a0ef484d5674a303d8b719971d431921c407bf501c31ccee217f32293</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10334-014-0447-2$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10334-014-0447-2$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24916487$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Simões, Rui V.</creatorcontrib><creatorcontrib>Ortuño, Juan E.</creatorcontrib><creatorcontrib>Bokacheva, Louisa</creatorcontrib><creatorcontrib>Candiota, Ana P.</creatorcontrib><creatorcontrib>Ledesma-Carbayo, Maria J.</creatorcontrib><creatorcontrib>Delgado-Goñi, Teresa</creatorcontrib><creatorcontrib>García-Martín, Maria L.</creatorcontrib><creatorcontrib>Santos, Andrés</creatorcontrib><creatorcontrib>Arús, Carles</creatorcontrib><title>Effect of acute hyperglycemia on moderately hypothermic GL261 mouse glioma monitored by T1-weighted DCE MRI</title><title>Magma (New York, N.Y.)</title><addtitle>Magn Reson Mater Phy</addtitle><addtitle>MAGMA</addtitle><description>Objective
We sought to evaluate the effects of acute hyperglycemia induced by intraperitoneal injection of glucose (2.7 g/kg) on vascular delivery to GL261 mouse gliomas kept at moderate hypothermia (~30 °C).
Materials and methods
Seven GL261 glioma-bearing mice were studied by T1-weighted DCE MRI before and after an injection of glucose (
n
= 4) or saline (
n
= 3). Maximum relative contrast enhancement (RCE) and initial area under the enhancement curve (IAUC) were determined in each pixel.
Results
The mean tumor parameter values showed no significant changes after injecting either saline (RCE −5.9 ± 5.0 %; IAUC −3.7 ± 3.6 %) or glucose (RCE −1.6 ± 9.0 %; IAUC +0.6 ± 6.4 %). Pixel-by-pixel analysis revealed small post-injection changes in RCE and IAUC between the glucose and saline groups, all within 13 % range of their baseline values.
Conclusion
Perturbing the metabolism of GL261 tumors kept at moderate hypothermia with hyperglycemia did not induce significant changes in the permeability/perfusion of these tumors. This is relevant for future studies with this model since regional differences in glucose accumulation could thus reflect basal heterogeneities in vasculature and/or metabolism of GL261 tumors.</description><subject>Acute Disease</subject><subject>Animals</subject><subject>Biology</subject><subject>Biomedical Engineering and Bioengineering</subject><subject>Brain Neoplasms - complications</subject><subject>Brain Neoplasms - pathology</subject><subject>Brain Neoplasms - therapy</subject><subject>Cell Line, Tumor</subject><subject>Computer Appl. in Life Sciences</subject><subject>Diffusion Magnetic Resonance Imaging - methods</subject><subject>Female</subject><subject>Glioma</subject><subject>Glucose</subject><subject>Health Informatics</subject><subject>Heterogeneity</subject><subject>Hyperglycemia</subject><subject>Hyperglycemia - complications</subject><subject>Hyperglycemia - pathology</subject><subject>Hypothermia</subject><subject>Hypothermia, Induced - methods</subject><subject>Imaging</subject><subject>Medicine & Public Health</subject><subject>Metabolism</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Pixels</subject><subject>Radiology</subject><subject>Reproducibility of Results</subject><subject>Research Article</subject><subject>Saline</subject><subject>Sensitivity and Specificity</subject><subject>Solid State Physics</subject><subject>Treatment Outcome</subject><subject>Tumor Burden</subject><subject>Tumors</subject><issn>0968-5243</issn><issn>1352-8661</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkUFv1DAQhS0EokvLD-CCfOQS8NiO7RzRspRKW1VC7dnyOuPdlCRe7ERV_j1ebeGIehhZo_fNk_UeIR-AfQbG9JcMTAhZMSgjpa74K7ICUfPKKAWvyYo1ylQ1l-KCvMv5kTEONRNvyQWXDShp9Ir82oSAfqIxUOfnCelhOWLa94vHoXM0jnSILSY3Yb-ctDgdMA2dp9dbrqCIc0a677s4uLKM3RQTtnS30HuonrDbH6ayfltv6O3PmyvyJrg-4_vn95I8fN_cr39U27vrm_XXbeVrxqdKt6INrjGOYZBGtrXS0gkmWrPT0DQaWimg4eAl07tQM_ACvEfkoIPgvBGX5NPZ95ji7xnzZIcue-x7N2L5rwVlasNO8xK0JNso9RJUAy_pS1NQOKM-xZwTBntM3eDSYoHZU3P23JwtzdlTc5aXm4_P9vNuwPbfxd-qCsDPQC7SuMdkH-OcxhLkf1z_AIOzoSo</recordid><startdate>20150401</startdate><enddate>20150401</enddate><creator>Simões, Rui V.</creator><creator>Ortuño, Juan E.</creator><creator>Bokacheva, Louisa</creator><creator>Candiota, Ana P.</creator><creator>Ledesma-Carbayo, Maria J.</creator><creator>Delgado-Goñi, Teresa</creator><creator>García-Martín, Maria L.</creator><creator>Santos, Andrés</creator><creator>Arús, Carles</creator><general>Springer Berlin Heidelberg</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7SC</scope><scope>7U5</scope><scope>JQ2</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope></search><sort><creationdate>20150401</creationdate><title>Effect of acute hyperglycemia on moderately hypothermic GL261 mouse glioma monitored by T1-weighted DCE MRI</title><author>Simões, Rui V. ; Ortuño, Juan E. ; Bokacheva, Louisa ; Candiota, Ana P. ; Ledesma-Carbayo, Maria J. ; Delgado-Goñi, Teresa ; García-Martín, Maria L. ; Santos, Andrés ; Arús, Carles</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c502t-7d3dfa98a0ef484d5674a303d8b719971d431921c407bf501c31ccee217f32293</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Acute Disease</topic><topic>Animals</topic><topic>Biology</topic><topic>Biomedical Engineering and Bioengineering</topic><topic>Brain Neoplasms - complications</topic><topic>Brain Neoplasms - pathology</topic><topic>Brain Neoplasms - therapy</topic><topic>Cell Line, Tumor</topic><topic>Computer Appl. in Life Sciences</topic><topic>Diffusion Magnetic Resonance Imaging - methods</topic><topic>Female</topic><topic>Glioma</topic><topic>Glucose</topic><topic>Health Informatics</topic><topic>Heterogeneity</topic><topic>Hyperglycemia</topic><topic>Hyperglycemia - complications</topic><topic>Hyperglycemia - pathology</topic><topic>Hypothermia</topic><topic>Hypothermia, Induced - methods</topic><topic>Imaging</topic><topic>Medicine & Public Health</topic><topic>Metabolism</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Pixels</topic><topic>Radiology</topic><topic>Reproducibility of Results</topic><topic>Research Article</topic><topic>Saline</topic><topic>Sensitivity and Specificity</topic><topic>Solid State Physics</topic><topic>Treatment Outcome</topic><topic>Tumor Burden</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Simões, Rui V.</creatorcontrib><creatorcontrib>Ortuño, Juan E.</creatorcontrib><creatorcontrib>Bokacheva, Louisa</creatorcontrib><creatorcontrib>Candiota, Ana P.</creatorcontrib><creatorcontrib>Ledesma-Carbayo, Maria J.</creatorcontrib><creatorcontrib>Delgado-Goñi, Teresa</creatorcontrib><creatorcontrib>García-Martín, Maria L.</creatorcontrib><creatorcontrib>Santos, Andrés</creatorcontrib><creatorcontrib>Arús, Carles</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>ProQuest Computer Science Collection</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><jtitle>Magma (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Simões, Rui V.</au><au>Ortuño, Juan E.</au><au>Bokacheva, Louisa</au><au>Candiota, Ana P.</au><au>Ledesma-Carbayo, Maria J.</au><au>Delgado-Goñi, Teresa</au><au>García-Martín, Maria L.</au><au>Santos, Andrés</au><au>Arús, Carles</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of acute hyperglycemia on moderately hypothermic GL261 mouse glioma monitored by T1-weighted DCE MRI</atitle><jtitle>Magma (New York, N.Y.)</jtitle><stitle>Magn Reson Mater Phy</stitle><addtitle>MAGMA</addtitle><date>2015-04-01</date><risdate>2015</risdate><volume>28</volume><issue>2</issue><spage>119</spage><epage>126</epage><pages>119-126</pages><issn>0968-5243</issn><eissn>1352-8661</eissn><abstract>Objective
We sought to evaluate the effects of acute hyperglycemia induced by intraperitoneal injection of glucose (2.7 g/kg) on vascular delivery to GL261 mouse gliomas kept at moderate hypothermia (~30 °C).
Materials and methods
Seven GL261 glioma-bearing mice were studied by T1-weighted DCE MRI before and after an injection of glucose (
n
= 4) or saline (
n
= 3). Maximum relative contrast enhancement (RCE) and initial area under the enhancement curve (IAUC) were determined in each pixel.
Results
The mean tumor parameter values showed no significant changes after injecting either saline (RCE −5.9 ± 5.0 %; IAUC −3.7 ± 3.6 %) or glucose (RCE −1.6 ± 9.0 %; IAUC +0.6 ± 6.4 %). Pixel-by-pixel analysis revealed small post-injection changes in RCE and IAUC between the glucose and saline groups, all within 13 % range of their baseline values.
Conclusion
Perturbing the metabolism of GL261 tumors kept at moderate hypothermia with hyperglycemia did not induce significant changes in the permeability/perfusion of these tumors. This is relevant for future studies with this model since regional differences in glucose accumulation could thus reflect basal heterogeneities in vasculature and/or metabolism of GL261 tumors.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>24916487</pmid><doi>10.1007/s10334-014-0447-2</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0968-5243 |
| ispartof | Magma (New York, N.Y.), 2015-04, Vol.28 (2), p.119-126 |
| issn | 0968-5243 1352-8661 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1685808580 |
| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Acute Disease Animals Biology Biomedical Engineering and Bioengineering Brain Neoplasms - complications Brain Neoplasms - pathology Brain Neoplasms - therapy Cell Line, Tumor Computer Appl. in Life Sciences Diffusion Magnetic Resonance Imaging - methods Female Glioma Glucose Health Informatics Heterogeneity Hyperglycemia Hyperglycemia - complications Hyperglycemia - pathology Hypothermia Hypothermia, Induced - methods Imaging Medicine & Public Health Metabolism Mice Mice, Inbred C57BL Pixels Radiology Reproducibility of Results Research Article Saline Sensitivity and Specificity Solid State Physics Treatment Outcome Tumor Burden Tumors |
| title | Effect of acute hyperglycemia on moderately hypothermic GL261 mouse glioma monitored by T1-weighted DCE MRI |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-13T03%3A45%3A43IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Effect%20of%20acute%20hyperglycemia%20on%20moderately%20hypothermic%20GL261%20mouse%20glioma%20monitored%20by%20T1-weighted%20DCE%20MRI&rft.jtitle=Magma%20(New%20York,%20N.Y.)&rft.au=Sim%C3%B5es,%20Rui%20V.&rft.date=2015-04-01&rft.volume=28&rft.issue=2&rft.spage=119&rft.epage=126&rft.pages=119-126&rft.issn=0968-5243&rft.eissn=1352-8661&rft_id=info:doi/10.1007/s10334-014-0447-2&rft_dat=%3Cproquest_cross%3E1671213548%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1671213548&rft_id=info:pmid/24916487&rfr_iscdi=true |