new virus-induced gene silencing vector based on Euphorbia mosaic virus-Yucatan peninsula for NPR1 silencing in Nicotiana benthamiana and Capsicum annuum var. Anaheim
Virus-induced gene silencing is based on the sequence-specific degradation of RNA. Here, a gene silencing vector derived from EuMV-YP, named pEuMV-YP:ΔAV1, was used to silence ChlI and NPR1 genes in Nicotiana benthamiana. The silencing of the ChlI transcripts was efficient in the stems, petioles and...
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| Veröffentlicht in: | Biotechnology letters 2013-05, Vol.35 (5), p.811-823 |
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| creator | Villanueva-Alonzo, Hernan J Us-Camas, Rosa Y López-Ochoa, Luisa A Robertson, Dominique Guerra-Peraza, Orlene Minero-García, Yereni Moreno-Valenzuela, Oscar A |
| description | Virus-induced gene silencing is based on the sequence-specific degradation of RNA. Here, a gene silencing vector derived from EuMV-YP, named pEuMV-YP:ΔAV1, was used to silence ChlI and NPR1 genes in Nicotiana benthamiana. The silencing of the ChlI transcripts was efficient in the stems, petioles and leaves as reflected in tissue bleaching and reduced transcript levels. The silencing was stable, reaching the flowers and fruits, and was observed throughout the life cycle of the plants. Additionally, the silencing of the NPR1 gene was efficient in both N. benthamiana and Capsicum annuum. After silencing, the plants’ viral symptoms increased to levels similar to those seen in wild-type plants. These results suggest that NPR1 plays a role in the compatible interactions of EuMV-YP N. benthamiana and EuMV-C. annum var. anaheim. |
| doi_str_mv | 10.1007/s10529-013-1146-1 |
| format | Article |
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Anaheim</title><source>MEDLINE</source><source>SpringerLink Journals - AutoHoldings</source><creator>Villanueva-Alonzo, Hernan J ; Us-Camas, Rosa Y ; López-Ochoa, Luisa A ; Robertson, Dominique ; Guerra-Peraza, Orlene ; Minero-García, Yereni ; Moreno-Valenzuela, Oscar A</creator><creatorcontrib>Villanueva-Alonzo, Hernan J ; Us-Camas, Rosa Y ; López-Ochoa, Luisa A ; Robertson, Dominique ; Guerra-Peraza, Orlene ; Minero-García, Yereni ; Moreno-Valenzuela, Oscar A</creatorcontrib><description>Virus-induced gene silencing is based on the sequence-specific degradation of RNA. Here, a gene silencing vector derived from EuMV-YP, named pEuMV-YP:ΔAV1, was used to silence ChlI and NPR1 genes in Nicotiana benthamiana. The silencing of the ChlI transcripts was efficient in the stems, petioles and leaves as reflected in tissue bleaching and reduced transcript levels. The silencing was stable, reaching the flowers and fruits, and was observed throughout the life cycle of the plants. Additionally, the silencing of the NPR1 gene was efficient in both N. benthamiana and Capsicum annuum. After silencing, the plants’ viral symptoms increased to levels similar to those seen in wild-type plants. These results suggest that NPR1 plays a role in the compatible interactions of EuMV-YP N. benthamiana and EuMV-C. annum var. anaheim.</description><identifier>ISSN: 0141-5492</identifier><identifier>EISSN: 1573-6776</identifier><identifier>DOI: 10.1007/s10529-013-1146-1</identifier><identifier>PMID: 23546940</identifier><language>eng</language><publisher>Dordrecht: Springer-Verlag</publisher><subject>Applied Microbiology ; Biochemistry ; Biomedical and Life Sciences ; Biotechnology ; Bleaching ; Capsicum - genetics ; Capsicum - metabolism ; Capsicum - virology ; Capsicum annuum ; Disease Resistance - genetics ; Euphorbia ; flowers ; fruits ; Gene Silencing ; Genes ; Genes, Plant - genetics ; Genetic Vectors - genetics ; Genomics ; Leaves ; Life Sciences ; Mathematical analysis ; Microbiology ; Mosaic Viruses - genetics ; Nicotiana - genetics ; Nicotiana - metabolism ; Nicotiana - virology ; Nicotiana benthamiana ; Original Research Paper ; petioles ; Plant biology ; Plant Diseases ; Plant Proteins - analysis ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Plants (organisms) ; Plasmids ; RNA ; Stems ; VAR ; Vectors (mathematics) ; Viruses</subject><ispartof>Biotechnology letters, 2013-05, Vol.35 (5), p.811-823</ispartof><rights>Springer Science+Business Media Dordrecht 2013</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c486t-ded981261912e6f1386a0fc3273e6903427261ca6140fdafebdc1faf120959e53</citedby><cites>FETCH-LOGICAL-c486t-ded981261912e6f1386a0fc3273e6903427261ca6140fdafebdc1faf120959e53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10529-013-1146-1$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10529-013-1146-1$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,777,781,27905,27906,41469,42538,51300</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23546940$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Villanueva-Alonzo, Hernan J</creatorcontrib><creatorcontrib>Us-Camas, Rosa Y</creatorcontrib><creatorcontrib>López-Ochoa, Luisa A</creatorcontrib><creatorcontrib>Robertson, Dominique</creatorcontrib><creatorcontrib>Guerra-Peraza, Orlene</creatorcontrib><creatorcontrib>Minero-García, Yereni</creatorcontrib><creatorcontrib>Moreno-Valenzuela, Oscar A</creatorcontrib><title>new virus-induced gene silencing vector based on Euphorbia mosaic virus-Yucatan peninsula for NPR1 silencing in Nicotiana benthamiana and Capsicum annuum var. Anaheim</title><title>Biotechnology letters</title><addtitle>Biotechnol Lett</addtitle><addtitle>Biotechnol Lett</addtitle><description>Virus-induced gene silencing is based on the sequence-specific degradation of RNA. Here, a gene silencing vector derived from EuMV-YP, named pEuMV-YP:ΔAV1, was used to silence ChlI and NPR1 genes in Nicotiana benthamiana. The silencing of the ChlI transcripts was efficient in the stems, petioles and leaves as reflected in tissue bleaching and reduced transcript levels. The silencing was stable, reaching the flowers and fruits, and was observed throughout the life cycle of the plants. Additionally, the silencing of the NPR1 gene was efficient in both N. benthamiana and Capsicum annuum. After silencing, the plants’ viral symptoms increased to levels similar to those seen in wild-type plants. These results suggest that NPR1 plays a role in the compatible interactions of EuMV-YP N. benthamiana and EuMV-C. annum var. anaheim.</description><subject>Applied Microbiology</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Bleaching</subject><subject>Capsicum - genetics</subject><subject>Capsicum - metabolism</subject><subject>Capsicum - virology</subject><subject>Capsicum annuum</subject><subject>Disease Resistance - genetics</subject><subject>Euphorbia</subject><subject>flowers</subject><subject>fruits</subject><subject>Gene Silencing</subject><subject>Genes</subject><subject>Genes, Plant - genetics</subject><subject>Genetic Vectors - genetics</subject><subject>Genomics</subject><subject>Leaves</subject><subject>Life Sciences</subject><subject>Mathematical analysis</subject><subject>Microbiology</subject><subject>Mosaic Viruses - genetics</subject><subject>Nicotiana - genetics</subject><subject>Nicotiana - metabolism</subject><subject>Nicotiana - virology</subject><subject>Nicotiana benthamiana</subject><subject>Original Research Paper</subject><subject>petioles</subject><subject>Plant biology</subject><subject>Plant Diseases</subject><subject>Plant Proteins - analysis</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Plants (organisms)</subject><subject>Plasmids</subject><subject>RNA</subject><subject>Stems</subject><subject>VAR</subject><subject>Vectors (mathematics)</subject><subject>Viruses</subject><issn>0141-5492</issn><issn>1573-6776</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNks1uEzEURi0EoiHwAGzAUjdspvjaHnu8rKLyI1UFAV2wGt14PImrjCfY4yBeiOfEaQKqWBQWlm3d832WpUPIc2BnwJh-nYDV3FQMRAUgVQUPyAxqLSqltXpIZgwkVLU0_IQ8SemGMWY004_JCRe1VEayGfkZ3He68zGnyocuW9fRlQuOJr9xwfqwojtnpzHSJaYyGwO9yNv1GJce6TAm9PaY_potThjo1gUfUt4g7Uvq6uMnuNPlA73ydpw8BqRLF6Y1DrdnDB1d4DZ5m4dyCblsO4xn9Dzg2vnhKXnU4ya5Z8d9Tq7fXHxZvKsuP7x9vzi_rKxs1FR1rjMNcAUGuFM9iEYh663gWjhlmJBcl6FFBZL1HfZu2VnosQfOTG1cLebk1aF3G8dv2aWpHXyybrPB4MacWlBN3TDNQfwbFTXnpqzmP1DZSKPhtvX0L_RmzDGUP-8LlVKsaXSh4EDZOKYUXd9uox8w_miBtXs12oMabVGj3atRwnPy4ticl4Pr_iR-u1AAfgBSGYWVi3eevqf15SHU49jiKvrUXn_mRbwim6y11PcSXNdMi1_mVNZ1</recordid><startdate>20130501</startdate><enddate>20130501</enddate><creator>Villanueva-Alonzo, Hernan J</creator><creator>Us-Camas, Rosa Y</creator><creator>López-Ochoa, Luisa A</creator><creator>Robertson, Dominique</creator><creator>Guerra-Peraza, Orlene</creator><creator>Minero-García, Yereni</creator><creator>Moreno-Valenzuela, Oscar A</creator><general>Springer-Verlag</general><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QR</scope><scope>7T7</scope><scope>7TB</scope><scope>7U5</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>L6V</scope><scope>L7M</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>Q9U</scope><scope>7X8</scope><scope>7QO</scope><scope>7TM</scope><scope>7U9</scope><scope>H94</scope><scope>RC3</scope></search><sort><creationdate>20130501</creationdate><title>new virus-induced gene silencing vector based on Euphorbia mosaic virus-Yucatan peninsula for NPR1 silencing in Nicotiana benthamiana and Capsicum annuum var. 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Anaheim</atitle><jtitle>Biotechnology letters</jtitle><stitle>Biotechnol Lett</stitle><addtitle>Biotechnol Lett</addtitle><date>2013-05-01</date><risdate>2013</risdate><volume>35</volume><issue>5</issue><spage>811</spage><epage>823</epage><pages>811-823</pages><issn>0141-5492</issn><eissn>1573-6776</eissn><abstract>Virus-induced gene silencing is based on the sequence-specific degradation of RNA. Here, a gene silencing vector derived from EuMV-YP, named pEuMV-YP:ΔAV1, was used to silence ChlI and NPR1 genes in Nicotiana benthamiana. The silencing of the ChlI transcripts was efficient in the stems, petioles and leaves as reflected in tissue bleaching and reduced transcript levels. The silencing was stable, reaching the flowers and fruits, and was observed throughout the life cycle of the plants. Additionally, the silencing of the NPR1 gene was efficient in both N. benthamiana and Capsicum annuum. After silencing, the plants’ viral symptoms increased to levels similar to those seen in wild-type plants. These results suggest that NPR1 plays a role in the compatible interactions of EuMV-YP N. benthamiana and EuMV-C. annum var. anaheim.</abstract><cop>Dordrecht</cop><pub>Springer-Verlag</pub><pmid>23546940</pmid><doi>10.1007/s10529-013-1146-1</doi><tpages>13</tpages></addata></record> |
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| ispartof | Biotechnology letters, 2013-05, Vol.35 (5), p.811-823 |
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| subjects | Applied Microbiology Biochemistry Biomedical and Life Sciences Biotechnology Bleaching Capsicum - genetics Capsicum - metabolism Capsicum - virology Capsicum annuum Disease Resistance - genetics Euphorbia flowers fruits Gene Silencing Genes Genes, Plant - genetics Genetic Vectors - genetics Genomics Leaves Life Sciences Mathematical analysis Microbiology Mosaic Viruses - genetics Nicotiana - genetics Nicotiana - metabolism Nicotiana - virology Nicotiana benthamiana Original Research Paper petioles Plant biology Plant Diseases Plant Proteins - analysis Plant Proteins - genetics Plant Proteins - metabolism Plants (organisms) Plasmids RNA Stems VAR Vectors (mathematics) Viruses |
| title | new virus-induced gene silencing vector based on Euphorbia mosaic virus-Yucatan peninsula for NPR1 silencing in Nicotiana benthamiana and Capsicum annuum var. Anaheim |
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