Arsenite Oxidation by Pseudomonas arsenicoxydans Immobilized on Zeolite and Its Potential Biotechnological Application
Pseudomonas arsenicoxydans has been recently described as a new arsenite oxidizing bacterial species. Arsenite detoxification activity by this species was determined by HPLC/HG/AAS. P. arsenicoxydans showed a high rate of As(III) conversion, particularly when immobilized (it oxidizes 100 % of 500 μg...
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| Veröffentlicht in: | Bulletin of environmental contamination and toxicology 2015-05, Vol.94 (5), p.667-673 |
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| creator | Valenzuela, Cristian Moraga, Ruben Leon, Carla Smith, Carlos T. Mondaca, Maria-Angelica Campos, Victor L. |
| description | Pseudomonas arsenicoxydans
has been recently described as a new arsenite oxidizing bacterial species. Arsenite detoxification activity by this species was determined by HPLC/HG/AAS.
P. arsenicoxydans
showed a high rate of As(III) conversion, particularly when immobilized (it oxidizes 100 % of 500 μg arsenite present in the medium after 48 of incubation). Arsenite oxidizing activity, mediated by a constitutive periplasmic enzyme, was determined following the transfer of reducing equivalents from arsenite to 2,4-dichlorophenolindophenol (DCIP) showing that approximately 75 % (0.173 µmol DCIP min
−1
mg
−1
) of the total activity (0.231 µmol DCIP min
−1
mg
−1
) was detected in the periplasmic fraction. Using PCR with primers specific for arsenite oxidase gene showed the presence of a gene encoding for arsenite oxidase in
P. arsenicoxydans
. Results show the potential biotechnological application of
P.
arsenicoxydans
as a candidate for detoxification of As(III). |
| doi_str_mv | 10.1007/s00128-015-1495-7 |
| format | Article |
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has been recently described as a new arsenite oxidizing bacterial species. Arsenite detoxification activity by this species was determined by HPLC/HG/AAS.
P. arsenicoxydans
showed a high rate of As(III) conversion, particularly when immobilized (it oxidizes 100 % of 500 μg arsenite present in the medium after 48 of incubation). Arsenite oxidizing activity, mediated by a constitutive periplasmic enzyme, was determined following the transfer of reducing equivalents from arsenite to 2,4-dichlorophenolindophenol (DCIP) showing that approximately 75 % (0.173 µmol DCIP min
−1
mg
−1
) of the total activity (0.231 µmol DCIP min
−1
mg
−1
) was detected in the periplasmic fraction. Using PCR with primers specific for arsenite oxidase gene showed the presence of a gene encoding for arsenite oxidase in
P. arsenicoxydans
. Results show the potential biotechnological application of
P.
arsenicoxydans
as a candidate for detoxification of As(III).</description><identifier>ISSN: 0007-4861</identifier><identifier>EISSN: 1432-0800</identifier><identifier>DOI: 10.1007/s00128-015-1495-7</identifier><identifier>PMID: 25673523</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Aquatic Pollution ; Arsenic ; Arsenites - metabolism ; Atomic absorption analysis ; Bacteria ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Biotechnology ; Chemical compounds ; Detoxification ; Earth and Environmental Science ; Ecotoxicology ; Environment ; Environmental Chemistry ; Environmental Health ; Environmental Pollutants - metabolism ; Genes ; Liquid chromatography ; Molecular Sequence Data ; Oxidase ; Oxidation ; Oxidation-Reduction ; Oxidoreductases - genetics ; Oxidoreductases - metabolism ; Pollution ; Pseudomonas ; Pseudomonas - genetics ; Pseudomonas - metabolism ; Sequence Analysis, DNA ; Soil Science & Conservation ; Spectroscopy ; Waste Water Technology ; Water Management ; Water Pollution Control ; Zeolites - chemistry</subject><ispartof>Bulletin of environmental contamination and toxicology, 2015-05, Vol.94 (5), p.667-673</ispartof><rights>Springer Science+Business Media New York 2015</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a531t-56071a7caf74b5e26a5e7e01aaefc44300d41ad6a8966b12868f1a7b7ea575073</citedby><cites>FETCH-LOGICAL-a531t-56071a7caf74b5e26a5e7e01aaefc44300d41ad6a8966b12868f1a7b7ea575073</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00128-015-1495-7$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00128-015-1495-7$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25673523$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Valenzuela, Cristian</creatorcontrib><creatorcontrib>Moraga, Ruben</creatorcontrib><creatorcontrib>Leon, Carla</creatorcontrib><creatorcontrib>Smith, Carlos T.</creatorcontrib><creatorcontrib>Mondaca, Maria-Angelica</creatorcontrib><creatorcontrib>Campos, Victor L.</creatorcontrib><title>Arsenite Oxidation by Pseudomonas arsenicoxydans Immobilized on Zeolite and Its Potential Biotechnological Application</title><title>Bulletin of environmental contamination and toxicology</title><addtitle>Bull Environ Contam Toxicol</addtitle><addtitle>Bull Environ Contam Toxicol</addtitle><description>Pseudomonas arsenicoxydans
has been recently described as a new arsenite oxidizing bacterial species. Arsenite detoxification activity by this species was determined by HPLC/HG/AAS.
P. arsenicoxydans
showed a high rate of As(III) conversion, particularly when immobilized (it oxidizes 100 % of 500 μg arsenite present in the medium after 48 of incubation). Arsenite oxidizing activity, mediated by a constitutive periplasmic enzyme, was determined following the transfer of reducing equivalents from arsenite to 2,4-dichlorophenolindophenol (DCIP) showing that approximately 75 % (0.173 µmol DCIP min
−1
mg
−1
) of the total activity (0.231 µmol DCIP min
−1
mg
−1
) was detected in the periplasmic fraction. Using PCR with primers specific for arsenite oxidase gene showed the presence of a gene encoding for arsenite oxidase in
P. arsenicoxydans
. Results show the potential biotechnological application of
P.
arsenicoxydans
as a candidate for detoxification of As(III).</description><subject>Aquatic Pollution</subject><subject>Arsenic</subject><subject>Arsenites - metabolism</subject><subject>Atomic absorption analysis</subject><subject>Bacteria</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Biotechnology</subject><subject>Chemical compounds</subject><subject>Detoxification</subject><subject>Earth and Environmental Science</subject><subject>Ecotoxicology</subject><subject>Environment</subject><subject>Environmental Chemistry</subject><subject>Environmental Health</subject><subject>Environmental Pollutants - metabolism</subject><subject>Genes</subject><subject>Liquid chromatography</subject><subject>Molecular Sequence Data</subject><subject>Oxidase</subject><subject>Oxidation</subject><subject>Oxidation-Reduction</subject><subject>Oxidoreductases - genetics</subject><subject>Oxidoreductases - metabolism</subject><subject>Pollution</subject><subject>Pseudomonas</subject><subject>Pseudomonas - genetics</subject><subject>Pseudomonas - metabolism</subject><subject>Sequence Analysis, DNA</subject><subject>Soil Science & Conservation</subject><subject>Spectroscopy</subject><subject>Waste Water Technology</subject><subject>Water Management</subject><subject>Water Pollution Control</subject><subject>Zeolites - chemistry</subject><issn>0007-4861</issn><issn>1432-0800</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNkU1v1DAQhi0EokvhB3BBlrhwCYwdfyTHpaKwUqX2ABcu1iSZFFeJvcRJ1e2vx2ELQkhInDyyn3nH9sPYSwFvBYB9lwCErAoQuhCq1oV9xDZClbKACuAx20CGClUZccKepXSTaV1J-ZSdSG1sqWW5YbfbKVHwM_HLO9_h7GPgzYFfJVq6OMaAieNPoo13hw5D4rtxjI0f_D11PMNfKQ5rO4aO7-bEr-JMYfY48Pc-l-23EId47du8sd3vh1ysM56zJz0OiV48rKfsy_mHz2efiovLj7uz7UWBuhRzoQ1YgbbF3qpGkzSoyRIIROpbpUqATgnsDFa1MU3-C1P1mW8sobYabHnK3hxz91P8vlCa3ehTS8OAgeKSnDCVtjVoU_0Hak2prJYio6__Qm_iMoX8kJWCWihVr4HiSLVTTGmi3u0nP-J0cALc6s8d_bmsxa3-3HrfVw_JSzNS97vjl7AMyCOQ8lG4pumP0f9M_QG5SKZP</recordid><startdate>20150501</startdate><enddate>20150501</enddate><creator>Valenzuela, Cristian</creator><creator>Moraga, Ruben</creator><creator>Leon, Carla</creator><creator>Smith, Carlos T.</creator><creator>Mondaca, Maria-Angelica</creator><creator>Campos, Victor L.</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7T7</scope><scope>7TK</scope><scope>7TV</scope><scope>7U7</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88I</scope><scope>8C1</scope><scope>8FD</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>P64</scope><scope>PATMY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PYCSY</scope><scope>Q9U</scope><scope>7QL</scope><scope>7ST</scope><scope>SOI</scope><scope>7SR</scope><scope>7SU</scope><scope>JG9</scope><scope>KR7</scope></search><sort><creationdate>20150501</creationdate><title>Arsenite Oxidation by Pseudomonas arsenicoxydans Immobilized on Zeolite and Its Potential Biotechnological Application</title><author>Valenzuela, Cristian ; Moraga, Ruben ; Leon, Carla ; Smith, Carlos T. ; Mondaca, Maria-Angelica ; Campos, Victor L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a531t-56071a7caf74b5e26a5e7e01aaefc44300d41ad6a8966b12868f1a7b7ea575073</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Aquatic Pollution</topic><topic>Arsenic</topic><topic>Arsenites - metabolism</topic><topic>Atomic absorption analysis</topic><topic>Bacteria</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - metabolism</topic><topic>Biotechnology</topic><topic>Chemical compounds</topic><topic>Detoxification</topic><topic>Earth and Environmental Science</topic><topic>Ecotoxicology</topic><topic>Environment</topic><topic>Environmental Chemistry</topic><topic>Environmental Health</topic><topic>Environmental Pollutants - metabolism</topic><topic>Genes</topic><topic>Liquid chromatography</topic><topic>Molecular Sequence Data</topic><topic>Oxidase</topic><topic>Oxidation</topic><topic>Oxidation-Reduction</topic><topic>Oxidoreductases - genetics</topic><topic>Oxidoreductases - metabolism</topic><topic>Pollution</topic><topic>Pseudomonas</topic><topic>Pseudomonas - genetics</topic><topic>Pseudomonas - metabolism</topic><topic>Sequence Analysis, DNA</topic><topic>Soil Science & Conservation</topic><topic>Spectroscopy</topic><topic>Waste Water Technology</topic><topic>Water Management</topic><topic>Water Pollution Control</topic><topic>Zeolites - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Valenzuela, Cristian</creatorcontrib><creatorcontrib>Moraga, Ruben</creatorcontrib><creatorcontrib>Leon, Carla</creatorcontrib><creatorcontrib>Smith, Carlos T.</creatorcontrib><creatorcontrib>Mondaca, Maria-Angelica</creatorcontrib><creatorcontrib>Campos, Victor L.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Pollution Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Environmental Science Collection</collection><collection>ProQuest Central Basic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environment Abstracts</collection><collection>Environment Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Environmental Engineering Abstracts</collection><collection>Materials Research Database</collection><collection>Civil Engineering Abstracts</collection><jtitle>Bulletin of environmental contamination and toxicology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Valenzuela, Cristian</au><au>Moraga, Ruben</au><au>Leon, Carla</au><au>Smith, Carlos T.</au><au>Mondaca, Maria-Angelica</au><au>Campos, Victor L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Arsenite Oxidation by Pseudomonas arsenicoxydans Immobilized on Zeolite and Its Potential Biotechnological Application</atitle><jtitle>Bulletin of environmental contamination and toxicology</jtitle><stitle>Bull Environ Contam Toxicol</stitle><addtitle>Bull Environ Contam Toxicol</addtitle><date>2015-05-01</date><risdate>2015</risdate><volume>94</volume><issue>5</issue><spage>667</spage><epage>673</epage><pages>667-673</pages><issn>0007-4861</issn><eissn>1432-0800</eissn><abstract>Pseudomonas arsenicoxydans
has been recently described as a new arsenite oxidizing bacterial species. Arsenite detoxification activity by this species was determined by HPLC/HG/AAS.
P. arsenicoxydans
showed a high rate of As(III) conversion, particularly when immobilized (it oxidizes 100 % of 500 μg arsenite present in the medium after 48 of incubation). Arsenite oxidizing activity, mediated by a constitutive periplasmic enzyme, was determined following the transfer of reducing equivalents from arsenite to 2,4-dichlorophenolindophenol (DCIP) showing that approximately 75 % (0.173 µmol DCIP min
−1
mg
−1
) of the total activity (0.231 µmol DCIP min
−1
mg
−1
) was detected in the periplasmic fraction. Using PCR with primers specific for arsenite oxidase gene showed the presence of a gene encoding for arsenite oxidase in
P. arsenicoxydans
. Results show the potential biotechnological application of
P.
arsenicoxydans
as a candidate for detoxification of As(III).</abstract><cop>New York</cop><pub>Springer US</pub><pmid>25673523</pmid><doi>10.1007/s00128-015-1495-7</doi><tpages>7</tpages></addata></record> |
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| ispartof | Bulletin of environmental contamination and toxicology, 2015-05, Vol.94 (5), p.667-673 |
| issn | 0007-4861 1432-0800 |
| language | eng |
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| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Aquatic Pollution Arsenic Arsenites - metabolism Atomic absorption analysis Bacteria Bacterial Proteins - genetics Bacterial Proteins - metabolism Biotechnology Chemical compounds Detoxification Earth and Environmental Science Ecotoxicology Environment Environmental Chemistry Environmental Health Environmental Pollutants - metabolism Genes Liquid chromatography Molecular Sequence Data Oxidase Oxidation Oxidation-Reduction Oxidoreductases - genetics Oxidoreductases - metabolism Pollution Pseudomonas Pseudomonas - genetics Pseudomonas - metabolism Sequence Analysis, DNA Soil Science & Conservation Spectroscopy Waste Water Technology Water Management Water Pollution Control Zeolites - chemistry |
| title | Arsenite Oxidation by Pseudomonas arsenicoxydans Immobilized on Zeolite and Its Potential Biotechnological Application |
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