Real-time analysis of the interaction of a multiple-epitope peptide with antibodies against classical swine fever virus using surface plasmon resonance

► In this paper, CSFV multi-epitope GST-BT21 was expressed in E. coli and SPR further demonstrated the high reactivity of the multiple-epitope peptide BT21 with antibodies in serum from pigs infected with CSFV. ► We report SPR to study the interactions of recombinant multiple-epitope fusion protein...

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Veröffentlicht in:	Journal of biotechnology 2012-10, Vol.161 (3), p.221-227
Hauptverfasser:	Hou, Xiangmin, Tian, Hong, Wu, Jinyan, Tao, Jiang, Chen, Yan, Yin, Shuanghui, Zhang, Keshan, Shang, Youjun, Liu, Xiangtao
Format:	Artikel
Sprache:	eng
Schlagworte:	Affinity agarose Amino Acid Sequence Animals Antibodies Antibodies, Viral - immunology Antigen–antibody interaction antiserum Binding affinity and binding kinetics biotechnology blood serum Blotting, Western Classical Swine Fever - immunology Classical Swine Fever - virology Classical swine fever virus Classical swine fever virus - chemistry Classical swine fever virus - genetics Classical swine fever virus - immunology Computer Systems Electrophoresis, Polyacrylamide Gel epitopes Epitopes - chemistry Epitopes - genetics Epitopes - immunology Escherichia coli Escherichia coli - metabolism Fever gene expression Genes, Viral - genetics Glutathione glycoproteins Hydrogen-Ion Concentration Immobilized Proteins immune response Kinetics Molecular Sequence Data neutralizing antibodies Peptides - chemistry Peptides - immunology Plasmids - metabolism Plasmons Protein Binding - immunology Proteins Recombinant Recombinant Fusion Proteins - isolation & purification Recombinant Fusion Proteins - metabolism recombinant proteins Reproduction Surface plasmon resonance Surface Plasmon Resonance - methods Surface Properties Swine Swine - immunology Swine - virology Western blotting
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