Real-time monitoring of voltage shift based on enzymatically released pyrophosphate using phenylboronic acid-immobilized gate field-effect transistor
Pyrophosphate (PPi) is ubiquitous in living cells and is often produced by enzymatic reactions, e.g., DNA synthesis by DNA polymerase. We have developed a novel detection system for the voltage shift associated with the change in PPi concentration resulting from an enzymatic reaction using a phenylb...
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| Veröffentlicht in: | Japanese Journal of Applied Physics 2014-04, Vol.53 (4S), p.4-1-04EL04-3 |
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| container_end_page | 1-04EL04-3 |
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| container_issue | 4S |
| container_start_page | 4 |
| container_title | Japanese Journal of Applied Physics |
| container_volume | 53 |
| creator | Nishida, Hirokazu Takahashi, Kiyofumi Tabuse, Yuki Kambara, Hideki Sakata, Toshiya |
| description | Pyrophosphate (PPi) is ubiquitous in living cells and is often produced by enzymatic reactions, e.g., DNA synthesis by DNA polymerase. We have developed a novel detection system for the voltage shift associated with the change in PPi concentration resulting from an enzymatic reaction using a phenylboronic acid (PBA)-coated gate field-effect transistor (FET), since PBA coating is effective for detecting ion accumulation associated with PPi production from enzymatic reactions. To detect enzymatic reactions more efficiently, we employed the enzyme-electrode conjugation method using specific peptide sequences, which are spontaneously tethered to a gold substrate. The combination of the enzyme-electrode conjugation method with the charge detection using the PBA-coated FET enables the effective detection of enzymatic reactions. |
| doi_str_mv | 10.7567/JJAP.53.04EL04 |
| format | Article |
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We have developed a novel detection system for the voltage shift associated with the change in PPi concentration resulting from an enzymatic reaction using a phenylboronic acid (PBA)-coated gate field-effect transistor (FET), since PBA coating is effective for detecting ion accumulation associated with PPi production from enzymatic reactions. To detect enzymatic reactions more efficiently, we employed the enzyme-electrode conjugation method using specific peptide sequences, which are spontaneously tethered to a gold substrate. 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The combination of the enzyme-electrode conjugation method with the charge detection using the PBA-coated FET enables the effective detection of enzymatic reactions.</description><subject>Conjugation</subject><subject>Deoxyribonucleic acid</subject><subject>DNA polymerase</subject><subject>Electric potential</subject><subject>Field effect transistors</subject><subject>Gates</subject><subject>Monitoring</subject><subject>Semiconductor devices</subject><subject>Voltage</subject><issn>0021-4922</issn><issn>1347-4065</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><recordid>eNp1kE9v1DAQxSNEJZbClbOPgJQl8Z84OVZVC1QrgdpytsbOeNcrJw62Fyn9HnxfErZHOI1G83tP815RvKurrRSN_HR3d_V9K9i24je7ir8oNjXjsuRVI14Wm6qidck7Sl8Vr1M6LmsjeL0pft8j-DK7AckQRpdDdOOeBEt-BZ9hjyQdnM1EQ8KehJHg-DQPkJ0B72cS0ePfyzTHMB1Cmg6QkZzSajIdcJy9DnHxNQSM60s3DEE7754WyX4lrUPfl2gtmkxyhDG5tPzwpriw4BO-fZ6XxY_bm8frL-Xu2-ev11e70vBK5hJZow2TtAHaWQMSLGO6pYJr1mLXGoMNY1yLlgLl2lqgAKLXddUhr6FDdlm8P_tOMfw8YcpqcMmg9zBiOCVVN62QspYdX9DtGTUxpBTRqim6AeKs6kqt_au1fyWYOve_CD6eBS5M6hhOcVyS_B_-8A_4eIRphfjDM6em3rI_wI2ZRQ</recordid><startdate>20140401</startdate><enddate>20140401</enddate><creator>Nishida, Hirokazu</creator><creator>Takahashi, Kiyofumi</creator><creator>Tabuse, Yuki</creator><creator>Kambara, Hideki</creator><creator>Sakata, Toshiya</creator><general>The Japan Society of Applied Physics</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7SP</scope><scope>7U5</scope><scope>8FD</scope><scope>H8D</scope><scope>L7M</scope></search><sort><creationdate>20140401</creationdate><title>Real-time monitoring of voltage shift based on enzymatically released pyrophosphate using phenylboronic acid-immobilized gate field-effect transistor</title><author>Nishida, Hirokazu ; Takahashi, Kiyofumi ; Tabuse, Yuki ; Kambara, Hideki ; Sakata, Toshiya</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c407t-e36bc3726a29fca7af33b8254b38e98cce6334b582a24bffa2aa5db109e41a9e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Conjugation</topic><topic>Deoxyribonucleic acid</topic><topic>DNA polymerase</topic><topic>Electric potential</topic><topic>Field effect transistors</topic><topic>Gates</topic><topic>Monitoring</topic><topic>Semiconductor devices</topic><topic>Voltage</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nishida, Hirokazu</creatorcontrib><creatorcontrib>Takahashi, Kiyofumi</creatorcontrib><creatorcontrib>Tabuse, Yuki</creatorcontrib><creatorcontrib>Kambara, Hideki</creatorcontrib><creatorcontrib>Sakata, Toshiya</creatorcontrib><collection>CrossRef</collection><collection>Electronics & Communications Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Aerospace Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Japanese Journal of Applied Physics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nishida, Hirokazu</au><au>Takahashi, Kiyofumi</au><au>Tabuse, Yuki</au><au>Kambara, Hideki</au><au>Sakata, Toshiya</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Real-time monitoring of voltage shift based on enzymatically released pyrophosphate using phenylboronic acid-immobilized gate field-effect transistor</atitle><jtitle>Japanese Journal of Applied Physics</jtitle><addtitle>Jpn. J. Appl. Phys</addtitle><date>2014-04-01</date><risdate>2014</risdate><volume>53</volume><issue>4S</issue><spage>4</spage><epage>1-04EL04-3</epage><pages>4-1-04EL04-3</pages><issn>0021-4922</issn><eissn>1347-4065</eissn><coden>JJAPB6</coden><abstract>Pyrophosphate (PPi) is ubiquitous in living cells and is often produced by enzymatic reactions, e.g., DNA synthesis by DNA polymerase. We have developed a novel detection system for the voltage shift associated with the change in PPi concentration resulting from an enzymatic reaction using a phenylboronic acid (PBA)-coated gate field-effect transistor (FET), since PBA coating is effective for detecting ion accumulation associated with PPi production from enzymatic reactions. To detect enzymatic reactions more efficiently, we employed the enzyme-electrode conjugation method using specific peptide sequences, which are spontaneously tethered to a gold substrate. The combination of the enzyme-electrode conjugation method with the charge detection using the PBA-coated FET enables the effective detection of enzymatic reactions.</abstract><pub>The Japan Society of Applied Physics</pub><doi>10.7567/JJAP.53.04EL04</doi><tpages>3</tpages></addata></record> |
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| ispartof | Japanese Journal of Applied Physics, 2014-04, Vol.53 (4S), p.4-1-04EL04-3 |
| issn | 0021-4922 1347-4065 |
| language | eng |
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| source | IOP Publishing Journals; Institute of Physics (IOP) Journals - HEAL-Link |
| subjects | Conjugation Deoxyribonucleic acid DNA polymerase Electric potential Field effect transistors Gates Monitoring Semiconductor devices Voltage |
| title | Real-time monitoring of voltage shift based on enzymatically released pyrophosphate using phenylboronic acid-immobilized gate field-effect transistor |
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