Intervention and therapeutic effect of siRNA-HDAC5 on abnormal histone modification in non-obese diabetic mice
To evaluate therapeutic eff ect of siRNA-HDAC5 on non-obese diabetic (NOD) mice by using small interference RNA (siRNA) technique to knock down the expression of HDAC5 in spleen CD4+ T cells. NOD mice, 12-weeks old, were randomly divided into 3 groups and were given normal saline, siRNA-Control or s...
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| Veröffentlicht in: | Zhong nan da xue xue bao. Journal of Central South University. Yi xue ban 2015-05, Vol.40 (5), p.464-470 |
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| container_title | Zhong nan da xue xue bao. Journal of Central South University. Yi xue ban |
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| creator | Ouyang, Lin Wang, Yanfei Liu, Lingjiao Peng, Youming Hou, Can |
| description | To evaluate therapeutic eff ect of siRNA-HDAC5 on non-obese diabetic (NOD) mice by using small interference RNA (siRNA) technique to knock down the expression of HDAC5 in spleen CD4+ T cells.
NOD mice, 12-weeks old, were randomly divided into 3 groups and were given normal saline, siRNA-Control or siRNA-HDAC5 through caudal vein injection. The spleens and other samples were collected at the 18th, 24th or 30th week. The blood glucose was tested by blood glucose meter. The urinary albumin and serum levels of IL-1, IL-6, IL-18, and TNF-α were detected by ELISA. The mRNA levels of CD11a, CCR5, and CX3CR1 in spleen CD4+ T cells were measured by quantitative Real-time PCR. The HDAC5 protein level in spleen CD4+ T cell was detected by Western blot.
Compared with the control group, the siRNA-HDAC5 group showed a significant decrease in blood glucose, urine albumin excretion rate, serum cytokine and the mRNA levels of CD11a, CCR5, and CX3CR1, consist with the decrease in protein level of HDAC5.
Inhibition of HDAC5 exp |
| doi_str_mv | 10.11817/j.issn.1672-7347.2015.05.002 |
| format | Article |
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NOD mice, 12-weeks old, were randomly divided into 3 groups and were given normal saline, siRNA-Control or siRNA-HDAC5 through caudal vein injection. The spleens and other samples were collected at the 18th, 24th or 30th week. The blood glucose was tested by blood glucose meter. The urinary albumin and serum levels of IL-1, IL-6, IL-18, and TNF-α were detected by ELISA. The mRNA levels of CD11a, CCR5, and CX3CR1 in spleen CD4+ T cells were measured by quantitative Real-time PCR. The HDAC5 protein level in spleen CD4+ T cell was detected by Western blot.
Compared with the control group, the siRNA-HDAC5 group showed a significant decrease in blood glucose, urine albumin excretion rate, serum cytokine and the mRNA levels of CD11a, CCR5, and CX3CR1, consist with the decrease in protein level of HDAC5.
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NOD mice, 12-weeks old, were randomly divided into 3 groups and were given normal saline, siRNA-Control or siRNA-HDAC5 through caudal vein injection. The spleens and other samples were collected at the 18th, 24th or 30th week. The blood glucose was tested by blood glucose meter. The urinary albumin and serum levels of IL-1, IL-6, IL-18, and TNF-α were detected by ELISA. The mRNA levels of CD11a, CCR5, and CX3CR1 in spleen CD4+ T cells were measured by quantitative Real-time PCR. The HDAC5 protein level in spleen CD4+ T cell was detected by Western blot.
Compared with the control group, the siRNA-HDAC5 group showed a significant decrease in blood glucose, urine albumin excretion rate, serum cytokine and the mRNA levels of CD11a, CCR5, and CX3CR1, consist with the decrease in protein level of HDAC5.
Inhibition of HDAC5 exp</description><subject>Animals</subject><subject>CD11a Antigen - metabolism</subject><subject>CD4-Positive T-Lymphocytes - metabolism</subject><subject>CX3C Chemokine Receptor 1</subject><subject>Cytokines - blood</subject><subject>Diabetes Mellitus, Experimental - genetics</subject><subject>Diabetes Mellitus, Experimental - therapy</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Histone Code</subject><subject>Histone Deacetylases - genetics</subject><subject>Mice</subject><subject>Mice, Inbred NOD</subject><subject>Random Allocation</subject><subject>Real-Time Polymerase Chain Reaction</subject><subject>Receptors, CCR5 - metabolism</subject><subject>Receptors, Chemokine - metabolism</subject><subject>RNA, Messenger - metabolism</subject><subject>RNA, Small Interfering - genetics</subject><subject>RNA, Small Interfering - therapeutic use</subject><subject>Spleen - cytology</subject><issn>1672-7347</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kF1LwzAUhnOhuDH3FyQ3gjet-ViS9nLMjw2Gguh1SdJTFmmT2qSC_95Op3DgfXl5eC4OQteU5JQWVN2-5y5Gn1OpWKb4SuWMUJGT6Qg7Q_P_fYaWMTpDSFmWXBbqAs2YJJwRJebI73yC4RN8csFj7WucDjDoHsbkLIamAZtwaHB0L0_rbHu33gh8BI0PQ6dbfHAxBQ-4C7VrnNU_GuexDz4LBiLg2mkDR1nnLFyi80a3EZanXKC3h_vXzTbbPz_uNut91lMmUyZrKy1XRJRAmdVEACUrzrWpS24sTBOsTKkKUQomuWgYyEYXU1NaWBCUL9DNr7cfwscIMVWdixbaVnsIY6yoLIQSkvJiQq9O6Gg6qKt-cJ0evqq_H_Fvd5prog</recordid><startdate>201505</startdate><enddate>201505</enddate><creator>Ouyang, Lin</creator><creator>Wang, Yanfei</creator><creator>Liu, Lingjiao</creator><creator>Peng, Youming</creator><creator>Hou, Can</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>201505</creationdate><title>Intervention and therapeutic effect of siRNA-HDAC5 on abnormal histone modification in non-obese diabetic mice</title><author>Ouyang, Lin ; Wang, Yanfei ; Liu, Lingjiao ; Peng, Youming ; Hou, Can</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p126t-6dc6c37059e12ca05e10433abd93bce2cae4b9785952635f2e6fa86357a5ce513</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>chi</language><creationdate>2015</creationdate><topic>Animals</topic><topic>CD11a Antigen - metabolism</topic><topic>CD4-Positive T-Lymphocytes - metabolism</topic><topic>CX3C Chemokine Receptor 1</topic><topic>Cytokines - blood</topic><topic>Diabetes Mellitus, Experimental - genetics</topic><topic>Diabetes Mellitus, Experimental - therapy</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Histone Code</topic><topic>Histone Deacetylases - genetics</topic><topic>Mice</topic><topic>Mice, Inbred NOD</topic><topic>Random Allocation</topic><topic>Real-Time Polymerase Chain Reaction</topic><topic>Receptors, CCR5 - metabolism</topic><topic>Receptors, Chemokine - metabolism</topic><topic>RNA, Messenger - metabolism</topic><topic>RNA, Small Interfering - genetics</topic><topic>RNA, Small Interfering - therapeutic use</topic><topic>Spleen - cytology</topic><toplevel>online_resources</toplevel><creatorcontrib>Ouyang, Lin</creatorcontrib><creatorcontrib>Wang, Yanfei</creatorcontrib><creatorcontrib>Liu, Lingjiao</creatorcontrib><creatorcontrib>Peng, Youming</creatorcontrib><creatorcontrib>Hou, Can</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Zhong nan da xue xue bao. Journal of Central South University. Yi xue ban</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ouyang, Lin</au><au>Wang, Yanfei</au><au>Liu, Lingjiao</au><au>Peng, Youming</au><au>Hou, Can</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Intervention and therapeutic effect of siRNA-HDAC5 on abnormal histone modification in non-obese diabetic mice</atitle><jtitle>Zhong nan da xue xue bao. Journal of Central South University. Yi xue ban</jtitle><addtitle>Zhong Nan Da Xue Xue Bao Yi Xue Ban</addtitle><date>2015-05</date><risdate>2015</risdate><volume>40</volume><issue>5</issue><spage>464</spage><epage>470</epage><pages>464-470</pages><issn>1672-7347</issn><abstract>To evaluate therapeutic eff ect of siRNA-HDAC5 on non-obese diabetic (NOD) mice by using small interference RNA (siRNA) technique to knock down the expression of HDAC5 in spleen CD4+ T cells.
NOD mice, 12-weeks old, were randomly divided into 3 groups and were given normal saline, siRNA-Control or siRNA-HDAC5 through caudal vein injection. The spleens and other samples were collected at the 18th, 24th or 30th week. The blood glucose was tested by blood glucose meter. The urinary albumin and serum levels of IL-1, IL-6, IL-18, and TNF-α were detected by ELISA. The mRNA levels of CD11a, CCR5, and CX3CR1 in spleen CD4+ T cells were measured by quantitative Real-time PCR. The HDAC5 protein level in spleen CD4+ T cell was detected by Western blot.
Compared with the control group, the siRNA-HDAC5 group showed a significant decrease in blood glucose, urine albumin excretion rate, serum cytokine and the mRNA levels of CD11a, CCR5, and CX3CR1, consist with the decrease in protein level of HDAC5.
Inhibition of HDAC5 exp</abstract><cop>China</cop><pmid>26032075</pmid><doi>10.11817/j.issn.1672-7347.2015.05.002</doi><tpages>7</tpages></addata></record> |
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| ispartof | Zhong nan da xue xue bao. Journal of Central South University. Yi xue ban, 2015-05, Vol.40 (5), p.464-470 |
| issn | 1672-7347 |
| language | chi |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals CD11a Antigen - metabolism CD4-Positive T-Lymphocytes - metabolism CX3C Chemokine Receptor 1 Cytokines - blood Diabetes Mellitus, Experimental - genetics Diabetes Mellitus, Experimental - therapy Enzyme-Linked Immunosorbent Assay Histone Code Histone Deacetylases - genetics Mice Mice, Inbred NOD Random Allocation Real-Time Polymerase Chain Reaction Receptors, CCR5 - metabolism Receptors, Chemokine - metabolism RNA, Messenger - metabolism RNA, Small Interfering - genetics RNA, Small Interfering - therapeutic use Spleen - cytology |
| title | Intervention and therapeutic effect of siRNA-HDAC5 on abnormal histone modification in non-obese diabetic mice |
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