Lactate monooxygenase. I. Expression of the mycobacterial gene in Escherichia coli and site-directed mutagenesis of lysine 266

Lactate monooxygenase utilizes oxygen in the conversion of L-lactate to acetate, CO2, and water. The gene for the enzyme from Mycobacterium smegmatis had been cloned into Escherichia coli (Giegel, D. A., Williams, C. H., Jr., and Massey, V. (1990) J. Biol. Chem. 265, 6626-6632) and the derived amino...

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Veröffentlicht in:	The Journal of biological chemistry 1994-03, Vol.269 (11), p.7982-7988
Hauptverfasser:	U MÃ¼h, V Massey, C H Williams, Jr
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Analytical, structural and metabolic biochemistry Base Sequence Binding Sites Biological and medical sciences Cloning, Molecular Enzymes and enzyme inhibitors Escherichia coli Escherichia coli - metabolism Fundamental and applied biological sciences. Psychology Gene Expression Lysine Mixed Function Oxygenases - biosynthesis Mixed Function Oxygenases - chemistry Mixed Function Oxygenases - genetics Molecular Sequence Data Mutagenesis, Site-Directed Mycobacterium - enzymology Mycobacterium - genetics Mycobacterium smegmatis Oligodeoxyribonucleotides Oxidation-Reduction Oxidoreductases Recombinant Proteins - biosynthesis Recombinant Proteins - chemistry Restriction Mapping Spectrophotometry Sulfites - metabolism Thermodynamics
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