Reconstruction of ethanol fermentation in permeabilized cells of the yeast Saccharomyces cerevisiae

Saccharomyces cerevisiae cells from a stationary culture were permeabilized with 1% toluene, 4% ethanol and 0.075% Triton X-100. Not only sugars but also ATP, NAD +, magnesium and inorganic phosphate must be simultaneously added to initiate the ethanol fermentation. The optimal pH for the fermentati...

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Veröffentlicht in:	Journal of fermentation and bioengineering 1995, Vol.79 (1), p.11-16
Hauptverfasser:	Takeshige, Kazuhiko, Ouchi, Kozo
Format:	Artikel
Sprache:	eng
Schlagworte:	ALCOHOLIC FERMENTATION ARSENIC COMPOUNDS Biological and medical sciences Biotechnology CELLS CELLULE CELULAS COMPOSE ARSENICAL COMPUESTOS ARSENICALES ESTIMULO ethanol fermentation FERMENTACION ALCOHOLICA FERMENTATION ALCOOLIQUE Fundamental and applied biological sciences. Psychology Methods. Procedures. Technologies Microbial engineering. Fermentation and microbial culture technology molasses NUTRIENT SOLUTIONS PERMEABILIDAD PERMEABILITE PERMEABILITY permeabilized cell POROSIDAD POROSITE POROSITY SACCHAROMYCES CEREVISIAE SACCHAROSE SOLUCIONES NUTRITIVAS SOLUTION NUTRITIVE STIMULI STIMULUS SUCROSA SUCROSE yeast
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