Structural and thermodynamic consequences of burying a charged residue within the hydrophobic core of T4 lysozyme

To determine the energetic and structural consequences of placing a charged group within the core of a protein, two "buried charge" mutants, Met 102---Lys (M102K) and Leu 133---Asp (L133D) were constructed in phage T4 lysozyme. Both proteins fold at neutral pH, although they are substantia...

Ausführliche Beschreibung