The ecotoxicological relevance of Anguilla anguilla L. as a proposed cytogenetic model for brackish-water genetic toxicological studies

The Sister Chromatid Exchange (SCE) Assay was specially adapted to Anguilla anguilla L. Cyclophosphamide (CP) was used as a standard on the SCE frequency study in Anguilla anguilla L. The 50 and 75% Bleached Kraft Pulp Mill Effluent (BKPME) was used to study the SCE frequency in Anguilla anguilla L....

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Veröffentlicht in:Science of the total environment 1993, Vol.134 (Suppl. Pts. 1-2), p.817-822
Hauptverfasser: Pacheco, M., Santos, M.A., van Der Gaag, M.A.
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container_issue Suppl. Pts. 1-2
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container_title Science of the total environment
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creator Pacheco, M.
Santos, M.A.
van Der Gaag, M.A.
description The Sister Chromatid Exchange (SCE) Assay was specially adapted to Anguilla anguilla L. Cyclophosphamide (CP) was used as a standard on the SCE frequency study in Anguilla anguilla L. The 50 and 75% Bleached Kraft Pulp Mill Effluent (BKPME) was used to study the SCE frequency in Anguilla anguilla L. in comparison with the standard cyclophosphamide results. The experimental results showed that the SCE assay is 33 h longer in Anguilla anguilla L. and also that the SCE frequency is lower in animals exposed either to cyclophosphamide or to 50 or 75% BKPME. However, further experiments with 75% BKPME showed an increase in the eel's SCE frequency compared to 50% BKPME, but it was still lower than the cyclophosphamide standard. In summary, the relevance of ecotoxicological tests, concerning the effect of industrial effluents containing potential genotoxic substances, should be based on the use of a wide range of species living in a particular habitat. The aim of this research work is to confirm the use of Anguilla anguilla L. as a genotoxic indicator, as part of the ecosystem, with a particular economic interest to this area of the country.
doi_str_mv 10.1016/S0048-9697(05)80087-7
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Cyclophosphamide (CP) was used as a standard on the SCE frequency study in Anguilla anguilla L. The 50 and 75% Bleached Kraft Pulp Mill Effluent (BKPME) was used to study the SCE frequency in Anguilla anguilla L. in comparison with the standard cyclophosphamide results. The experimental results showed that the SCE assay is 33 h longer in Anguilla anguilla L. and also that the SCE frequency is lower in animals exposed either to cyclophosphamide or to 50 or 75% BKPME. However, further experiments with 75% BKPME showed an increase in the eel's SCE frequency compared to 50% BKPME, but it was still lower than the cyclophosphamide standard. In summary, the relevance of ecotoxicological tests, concerning the effect of industrial effluents containing potential genotoxic substances, should be based on the use of a wide range of species living in a particular habitat. 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subjects Anguilla
Anguilla anguilla
Animal, plant and microbial ecology
Applied ecology
Biological and medical sciences
Brackish
cytogenetics
Ecotoxicology, biological effects of pollution
fish
Fresh water environment
Freshwater
Fundamental and applied biological sciences. Psychology
genotoxicity
Marine
pulp mill
sister chromatid exchange
title The ecotoxicological relevance of Anguilla anguilla L. as a proposed cytogenetic model for brackish-water genetic toxicological studies
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