Potassium ions and changes in bacterial DNA supercoiling under osmotic stress
Escherichia coli transiently increases both the [ATP]/[ADP] ratio and the negative supercoiling of plasmid DNA when it is shifted to high osmolarity. Here we report that a mutant lacking all saturable K+ transport systems increases the negative supercoiling of the plasmid DNA under upshock but canno...
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Veröffentlicht in: | FEMS microbiology letters 1992-12, Vol.99 (2‐3), p.159-164 |
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creator | Meury, Jean Kohiyama, Masamichi |
description | Escherichia coli transiently increases both the [ATP]/[ADP] ratio and the negative supercoiling of plasmid DNA when it is shifted to high osmolarity. Here we report that a mutant lacking all saturable K+ transport systems increases the negative supercoiling of the plasmid DNA under upshock but cannot further relax DNA. The mutant dnaK756 behaves like the K+ transport mutant. |
doi_str_mv | 10.1111/j.1574-6968.1992.tb05559.x |
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Here we report that a mutant lacking all saturable K+ transport systems increases the negative supercoiling of the plasmid DNA under upshock but cannot further relax DNA. The mutant dnaK756 behaves like the K+ transport mutant.</description><subject>Action of physical and chemical agents on bacteria</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>DNA supercoiling</subject><subject>DnaK</subject><subject>Escherichia coli</subject><subject>Fundamental and applied biological sciences. 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language | eng |
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source | Oxford University Press Journals Digital Archive legacy; Wiley Online Library Journals Frontfile Complete; Alma/SFX Local Collection |
subjects | Action of physical and chemical agents on bacteria Bacteriology Biological and medical sciences DNA supercoiling DnaK Escherichia coli Fundamental and applied biological sciences. Psychology Microbiology Osmotic upshock Potassium ions |
title | Potassium ions and changes in bacterial DNA supercoiling under osmotic stress |
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