Potassium ions and changes in bacterial DNA supercoiling under osmotic stress

Escherichia coli transiently increases both the [ATP]/[ADP] ratio and the negative supercoiling of plasmid DNA when it is shifted to high osmolarity. Here we report that a mutant lacking all saturable K+ transport systems increases the negative supercoiling of the plasmid DNA under upshock but canno...

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Veröffentlicht in:FEMS microbiology letters 1992-12, Vol.99 (2‐3), p.159-164
Hauptverfasser: Meury, Jean, Kohiyama, Masamichi
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Kohiyama, Masamichi
description Escherichia coli transiently increases both the [ATP]/[ADP] ratio and the negative supercoiling of plasmid DNA when it is shifted to high osmolarity. Here we report that a mutant lacking all saturable K+ transport systems increases the negative supercoiling of the plasmid DNA under upshock but cannot further relax DNA. The mutant dnaK756 behaves like the K+ transport mutant.
doi_str_mv 10.1111/j.1574-6968.1992.tb05559.x
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source Oxford University Press Journals Digital Archive legacy; Wiley Online Library Journals Frontfile Complete; Alma/SFX Local Collection
subjects Action of physical and chemical agents on bacteria
Bacteriology
Biological and medical sciences
DNA supercoiling
DnaK
Escherichia coli
Fundamental and applied biological sciences. Psychology
Microbiology
Osmotic upshock
Potassium ions
title Potassium ions and changes in bacterial DNA supercoiling under osmotic stress
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