Expression of TGMV antisense RNA in transgenic tobacco inhibits replication of BCTV but not ACMV geminiviruses
Transgenic tobacco plants expressing an antisense RNA targeted against tomato golden mosaic virus (TGMV) show reduced/no symptoms and viral DNA accumulation upon TGMV infection [5]. The targeted region includes the AL1 gene, encoding an essential viral replication protein. This DNA sequence is conse...
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| Veröffentlicht in: | Plant molecular biology 1994-01, Vol.24 (1), p.241-248 |
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| container_title | Plant molecular biology |
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| creator | Bejarano, E.R. (Imperial Coll. of Science, London (United Kingdom). Dept. of Biochemistry) Lichtenstein, C.P |
| description | Transgenic tobacco plants expressing an antisense RNA targeted against tomato golden mosaic virus (TGMV) show reduced/no symptoms and viral DNA accumulation upon TGMV infection [5]. The targeted region includes the AL1 gene, encoding an essential viral replication protein. This DNA sequence is conserved in various other geminiviruses, suggesting they too might show inhibition of replication in these plants. We infected leaf material with African cassava mosaic virus (ACMV) and beet curly top virus (BTCV) and saw a 4-fold reduction of BCTV, but not ACMV, DNA accumulation, compared to controls. The equivalent regions of BCTV and ACMV show similar overall homology to the TGMV target (63% and 64% respectively), but within this, BCTV displays a 280 nucleotide region of high homology (82%). In contrast, for ACMV, the homology is more dispersed. This indicates that a critical stretch of good complementarity is needed to block expression of the target mRNA, that is effective even within along antisense transcript. These studies indicate the potential for developing a multifunctional antisense cassette. |
| doi_str_mv | 10.1007/BF00040592 |
| format | Article |
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(Imperial Coll. of Science, London (United Kingdom). Dept. of Biochemistry) ; Lichtenstein, C.P</creator><creatorcontrib>Bejarano, E.R. (Imperial Coll. of Science, London (United Kingdom). Dept. of Biochemistry) ; Lichtenstein, C.P</creatorcontrib><description>Transgenic tobacco plants expressing an antisense RNA targeted against tomato golden mosaic virus (TGMV) show reduced/no symptoms and viral DNA accumulation upon TGMV infection [5]. The targeted region includes the AL1 gene, encoding an essential viral replication protein. This DNA sequence is conserved in various other geminiviruses, suggesting they too might show inhibition of replication in these plants. We infected leaf material with African cassava mosaic virus (ACMV) and beet curly top virus (BTCV) and saw a 4-fold reduction of BCTV, but not ACMV, DNA accumulation, compared to controls. The equivalent regions of BCTV and ACMV show similar overall homology to the TGMV target (63% and 64% respectively), but within this, BCTV displays a 280 nucleotide region of high homology (82%). In contrast, for ACMV, the homology is more dispersed. This indicates that a critical stretch of good complementarity is needed to block expression of the target mRNA, that is effective even within along antisense transcript. These studies indicate the potential for developing a multifunctional antisense cassette.</description><identifier>ISSN: 0167-4412</identifier><identifier>EISSN: 1573-5028</identifier><identifier>DOI: 10.1007/BF00040592</identifier><identifier>PMID: 8111023</identifier><language>eng</language><publisher>Netherlands</publisher><subject>ADN ; African cassava mosaic virus ; ARN ; beet curly top virus ; Blotting, Southern ; Cloning, Molecular ; DNA ; Geminiviridae - genetics ; Geminiviridae - physiology ; GEMINIVIRUS ; GEMINIVIRUSES ; GENE ; GENES ; NICOTIANA ; Nicotiana - microbiology ; Nicotiana tabacum ; NUCLEOTIDE SEQUENCE ; PLANT VIRUSES ; PLANTAS TRANSGENICAS ; PLANTE TRANSGENIQUE ; Plants, Genetically Modified ; Plants, Toxic ; RNA ; RNA, Antisense - biosynthesis ; RNA, Viral - biosynthesis ; SECUENCIA NUCLEICA ; SEQUENCE NUCLEIQUE ; Tobacco Mosaic Virus - genetics ; tomato golden mosaic virus ; TRANSGENIC PLANTS ; VIRUS DE LAS PLANTAS ; VIRUS DES VEGETAUX ; Virus Replication - genetics</subject><ispartof>Plant molecular biology, 1994-01, Vol.24 (1), p.241-248</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c332t-baa753e73e38203c231701cbefb42672f54a19d1f51afe6588da224cc03a8bf93</citedby><cites>FETCH-LOGICAL-c332t-baa753e73e38203c231701cbefb42672f54a19d1f51afe6588da224cc03a8bf93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8111023$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bejarano, E.R. (Imperial Coll. of Science, London (United Kingdom). Dept. of Biochemistry)</creatorcontrib><creatorcontrib>Lichtenstein, C.P</creatorcontrib><title>Expression of TGMV antisense RNA in transgenic tobacco inhibits replication of BCTV but not ACMV geminiviruses</title><title>Plant molecular biology</title><addtitle>Plant Mol Biol</addtitle><description>Transgenic tobacco plants expressing an antisense RNA targeted against tomato golden mosaic virus (TGMV) show reduced/no symptoms and viral DNA accumulation upon TGMV infection [5]. The targeted region includes the AL1 gene, encoding an essential viral replication protein. This DNA sequence is conserved in various other geminiviruses, suggesting they too might show inhibition of replication in these plants. We infected leaf material with African cassava mosaic virus (ACMV) and beet curly top virus (BTCV) and saw a 4-fold reduction of BCTV, but not ACMV, DNA accumulation, compared to controls. The equivalent regions of BCTV and ACMV show similar overall homology to the TGMV target (63% and 64% respectively), but within this, BCTV displays a 280 nucleotide region of high homology (82%). In contrast, for ACMV, the homology is more dispersed. This indicates that a critical stretch of good complementarity is needed to block expression of the target mRNA, that is effective even within along antisense transcript. These studies indicate the potential for developing a multifunctional antisense cassette.</description><subject>ADN</subject><subject>African cassava mosaic virus</subject><subject>ARN</subject><subject>beet curly top virus</subject><subject>Blotting, Southern</subject><subject>Cloning, Molecular</subject><subject>DNA</subject><subject>Geminiviridae - genetics</subject><subject>Geminiviridae - physiology</subject><subject>GEMINIVIRUS</subject><subject>GEMINIVIRUSES</subject><subject>GENE</subject><subject>GENES</subject><subject>NICOTIANA</subject><subject>Nicotiana - microbiology</subject><subject>Nicotiana tabacum</subject><subject>NUCLEOTIDE SEQUENCE</subject><subject>PLANT VIRUSES</subject><subject>PLANTAS TRANSGENICAS</subject><subject>PLANTE TRANSGENIQUE</subject><subject>Plants, Genetically Modified</subject><subject>Plants, Toxic</subject><subject>RNA</subject><subject>RNA, Antisense - biosynthesis</subject><subject>RNA, Viral - biosynthesis</subject><subject>SECUENCIA NUCLEICA</subject><subject>SEQUENCE NUCLEIQUE</subject><subject>Tobacco Mosaic Virus - genetics</subject><subject>tomato golden mosaic virus</subject><subject>TRANSGENIC PLANTS</subject><subject>VIRUS DE LAS PLANTAS</subject><subject>VIRUS DES VEGETAUX</subject><subject>Virus Replication - genetics</subject><issn>0167-4412</issn><issn>1573-5028</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkEtLAzEURoMotVY3LgUhKxfCaB7z6rItbRVqBandDpn0pkbapOZmRP-9Iy26unC_w1kcQi45u-OMFffDCWMsZVlfHJEuzwqZZEyUx6TLeF4kacrFKTlDfGesxWXeIZ2Sc86E7BI3_toFQLTeUW_oYvq0pMpFi-AQ6Mt8QK2jMSiHa3BW0-hrpbVvv2-2thFpgN3GahUPguFosaR1E6nzkQ5GrW0NW-vspw0NAp6TE6M2CBeH2yOvk_Fi9JDMnqePo8Es0VKKmNRKFZmEQoIsBZNaSF4wrmswdSryQpgsVby_4ibjykCeleVKCZFqzaQqa9OXPXKz9-6C_2gAY7W1qGGzUQ58gxXPyzRvi7Xg7R7UwSMGMNUu2K0K3xVn1W_c6j9uC18frE29hdUfeqjZ7lf73ShfqXWwWM1n_bSduJQ_B5x7wg</recordid><startdate>199401</startdate><enddate>199401</enddate><creator>Bejarano, E.R. 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Dept. of Biochemistry) ; Lichtenstein, C.P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c332t-baa753e73e38203c231701cbefb42672f54a19d1f51afe6588da224cc03a8bf93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>ADN</topic><topic>African cassava mosaic virus</topic><topic>ARN</topic><topic>beet curly top virus</topic><topic>Blotting, Southern</topic><topic>Cloning, Molecular</topic><topic>DNA</topic><topic>Geminiviridae - genetics</topic><topic>Geminiviridae - physiology</topic><topic>GEMINIVIRUS</topic><topic>GEMINIVIRUSES</topic><topic>GENE</topic><topic>GENES</topic><topic>NICOTIANA</topic><topic>Nicotiana - microbiology</topic><topic>Nicotiana tabacum</topic><topic>NUCLEOTIDE SEQUENCE</topic><topic>PLANT VIRUSES</topic><topic>PLANTAS TRANSGENICAS</topic><topic>PLANTE TRANSGENIQUE</topic><topic>Plants, Genetically Modified</topic><topic>Plants, Toxic</topic><topic>RNA</topic><topic>RNA, Antisense - biosynthesis</topic><topic>RNA, Viral - biosynthesis</topic><topic>SECUENCIA NUCLEICA</topic><topic>SEQUENCE NUCLEIQUE</topic><topic>Tobacco Mosaic Virus - genetics</topic><topic>tomato golden mosaic virus</topic><topic>TRANSGENIC PLANTS</topic><topic>VIRUS DE LAS PLANTAS</topic><topic>VIRUS DES VEGETAUX</topic><topic>Virus Replication - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bejarano, E.R. (Imperial Coll. of Science, London (United Kingdom). Dept. of Biochemistry)</creatorcontrib><creatorcontrib>Lichtenstein, C.P</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Plant molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bejarano, E.R. (Imperial Coll. of Science, London (United Kingdom). Dept. of Biochemistry)</au><au>Lichtenstein, C.P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of TGMV antisense RNA in transgenic tobacco inhibits replication of BCTV but not ACMV geminiviruses</atitle><jtitle>Plant molecular biology</jtitle><addtitle>Plant Mol Biol</addtitle><date>1994-01</date><risdate>1994</risdate><volume>24</volume><issue>1</issue><spage>241</spage><epage>248</epage><pages>241-248</pages><issn>0167-4412</issn><eissn>1573-5028</eissn><abstract>Transgenic tobacco plants expressing an antisense RNA targeted against tomato golden mosaic virus (TGMV) show reduced/no symptoms and viral DNA accumulation upon TGMV infection [5]. The targeted region includes the AL1 gene, encoding an essential viral replication protein. This DNA sequence is conserved in various other geminiviruses, suggesting they too might show inhibition of replication in these plants. We infected leaf material with African cassava mosaic virus (ACMV) and beet curly top virus (BTCV) and saw a 4-fold reduction of BCTV, but not ACMV, DNA accumulation, compared to controls. The equivalent regions of BCTV and ACMV show similar overall homology to the TGMV target (63% and 64% respectively), but within this, BCTV displays a 280 nucleotide region of high homology (82%). In contrast, for ACMV, the homology is more dispersed. This indicates that a critical stretch of good complementarity is needed to block expression of the target mRNA, that is effective even within along antisense transcript. These studies indicate the potential for developing a multifunctional antisense cassette.</abstract><cop>Netherlands</cop><pmid>8111023</pmid><doi>10.1007/BF00040592</doi><tpages>8</tpages></addata></record> |
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| issn | 0167-4412 1573-5028 |
| language | eng |
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| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | ADN African cassava mosaic virus ARN beet curly top virus Blotting, Southern Cloning, Molecular DNA Geminiviridae - genetics Geminiviridae - physiology GEMINIVIRUS GEMINIVIRUSES GENE GENES NICOTIANA Nicotiana - microbiology Nicotiana tabacum NUCLEOTIDE SEQUENCE PLANT VIRUSES PLANTAS TRANSGENICAS PLANTE TRANSGENIQUE Plants, Genetically Modified Plants, Toxic RNA RNA, Antisense - biosynthesis RNA, Viral - biosynthesis SECUENCIA NUCLEICA SEQUENCE NUCLEIQUE Tobacco Mosaic Virus - genetics tomato golden mosaic virus TRANSGENIC PLANTS VIRUS DE LAS PLANTAS VIRUS DES VEGETAUX Virus Replication - genetics |
| title | Expression of TGMV antisense RNA in transgenic tobacco inhibits replication of BCTV but not ACMV geminiviruses |
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