Automated metaphase finding: an assessment of the efficiency of the METAFER2 system in a routine mutagenicity assay
The efficiency of the automated metaphase finding system METAFER2 is assessed in a routine mutagenicity assay using an aneuploid rat liver cell line treated with various promutagens. Data sets generated by automated and manual selection of metaphases are compared. It is demonstrated that METAFER2 ro...
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| Veröffentlicht in: | Mutation Research 1995-02, Vol.334 (1), p.97-102 |
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| container_title | Mutation Research |
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| creator | Huber, R. Kulka, U. Lörch, Th Braselmann, H. Bauchinger, M. |
| description | The efficiency of the automated metaphase finding system METAFER2 is assessed in a routine mutagenicity assay using an aneuploid rat liver cell line treated with various promutagens. Data sets generated by automated and manual selection of metaphases are compared. It is demonstrated that METAFER2 routinely allows an efficient automatic identification of metaphases not only in lymphocyte preparations, but also in preparation from mammalian cell lines with varying chromosome numbers. Although larger slide areas are required for automated compared to manual metaphase scanning, the automatic system is faster by a factor of about 5. The interactive visual elimination of metaphases of insufficient quality is an easy and fast procedure.
METAFER2 allows an unbiased selection of metaphases irrespective of their appearance as homogeneously stained first or harlequin-staines second division cells. Random selection of metaphases is neither influenced by various structural chromosome changes nor by increased frequencies of sister-chromatid exchanges. |
| doi_str_mv | 10.1016/0165-1161(95)90035-7 |
| format | Article |
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METAFER2 allows an unbiased selection of metaphases irrespective of their appearance as homogeneously stained first or harlequin-staines second division cells. Random selection of metaphases is neither influenced by various structural chromosome changes nor by increased frequencies of sister-chromatid exchanges.</description><subject>Aneuploidy</subject><subject>Animals</subject><subject>Automated metaphase finding</subject><subject>Automation</subject><subject>Biological and medical sciences</subject><subject>Cell Line</subject><subject>Chemical mutagenesis</subject><subject>Chromosome Aberrations</subject><subject>Evaluation Studies as Topic</subject><subject>Image analysis</subject><subject>Image Processing, Computer-Assisted</subject><subject>Medical sciences</subject><subject>METAFER2</subject><subject>Metaphase</subject><subject>Mutagenicity Tests - methods</subject><subject>Mutagens - toxicity</subject><subject>Rats</subject><subject>Sister Chromatid Exchange</subject><subject>Time Factors</subject><subject>Toxicology</subject><issn>0165-1161</issn><issn>0027-5107</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEFrFDEUx-eg1Fr9Bgo5iOhhNJkkMxMPhaVsq1ARpJ7D28xLG9nJrHkZYb692e52jx7Cg_d-_z_hV1VvBP8kuGg_l6drIVrxweiPhnOp6-5ZdX5av6heEv3mXLW66c-qs64MqeR5Ras5TyNkHNiIGXYPQMh8iEOI918YRAZESDRizGzyLD8gQ--DCxjd8rT5vr5bXa9_NowWyjiyUGIsTXMOEdk4Z7jHWCJ52bfB8qp67mFL-Po4L6pf1-u7q6_17Y-bb1er29qpXuYapVfetW7wre7NoETjjW-4aVtsNqbzYLhRnRFNg43sPYB2G1ASvBZgem7kRfX-0LtL058ZKdsxkMPtFiJOM1nR9koXroDqALo0ESX0dpfCCGmxgtu9X7sXafcirdH20a_tSuztsX_ejDicQke55f7ueAdysPUJogt0wqRUou10wS4PGBYXfwMmS496cQgJXbbDFP7_j3823JkI</recordid><startdate>19950201</startdate><enddate>19950201</enddate><creator>Huber, R.</creator><creator>Kulka, U.</creator><creator>Lörch, Th</creator><creator>Braselmann, H.</creator><creator>Bauchinger, M.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>19950201</creationdate><title>Automated metaphase finding: an assessment of the efficiency of the METAFER2 system in a routine mutagenicity assay</title><author>Huber, R. ; Kulka, U. ; Lörch, Th ; Braselmann, H. ; Bauchinger, M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c483t-e3f4fc6cdf6589d412f9f20966e2b97fa909479122e238faa5cba43af51a98093</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Aneuploidy</topic><topic>Animals</topic><topic>Automated metaphase finding</topic><topic>Automation</topic><topic>Biological and medical sciences</topic><topic>Cell Line</topic><topic>Chemical mutagenesis</topic><topic>Chromosome Aberrations</topic><topic>Evaluation Studies as Topic</topic><topic>Image analysis</topic><topic>Image Processing, Computer-Assisted</topic><topic>Medical sciences</topic><topic>METAFER2</topic><topic>Metaphase</topic><topic>Mutagenicity Tests - methods</topic><topic>Mutagens - toxicity</topic><topic>Rats</topic><topic>Sister Chromatid Exchange</topic><topic>Time Factors</topic><topic>Toxicology</topic><toplevel>online_resources</toplevel><creatorcontrib>Huber, R.</creatorcontrib><creatorcontrib>Kulka, U.</creatorcontrib><creatorcontrib>Lörch, Th</creatorcontrib><creatorcontrib>Braselmann, H.</creatorcontrib><creatorcontrib>Bauchinger, M.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Mutation Research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Huber, R.</au><au>Kulka, U.</au><au>Lörch, Th</au><au>Braselmann, H.</au><au>Bauchinger, M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Automated metaphase finding: an assessment of the efficiency of the METAFER2 system in a routine mutagenicity assay</atitle><jtitle>Mutation Research</jtitle><addtitle>Mutat Res</addtitle><date>1995-02-01</date><risdate>1995</risdate><volume>334</volume><issue>1</issue><spage>97</spage><epage>102</epage><pages>97-102</pages><issn>0165-1161</issn><issn>0027-5107</issn><abstract>The efficiency of the automated metaphase finding system METAFER2 is assessed in a routine mutagenicity assay using an aneuploid rat liver cell line treated with various promutagens. Data sets generated by automated and manual selection of metaphases are compared. It is demonstrated that METAFER2 routinely allows an efficient automatic identification of metaphases not only in lymphocyte preparations, but also in preparation from mammalian cell lines with varying chromosome numbers. Although larger slide areas are required for automated compared to manual metaphase scanning, the automatic system is faster by a factor of about 5. The interactive visual elimination of metaphases of insufficient quality is an easy and fast procedure.
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| fulltext | fulltext |
| identifier | ISSN: 0165-1161 |
| ispartof | Mutation Research, 1995-02, Vol.334 (1), p.97-102 |
| issn | 0165-1161 0027-5107 |
| language | eng |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Aneuploidy Animals Automated metaphase finding Automation Biological and medical sciences Cell Line Chemical mutagenesis Chromosome Aberrations Evaluation Studies as Topic Image analysis Image Processing, Computer-Assisted Medical sciences METAFER2 Metaphase Mutagenicity Tests - methods Mutagens - toxicity Rats Sister Chromatid Exchange Time Factors Toxicology |
| title | Automated metaphase finding: an assessment of the efficiency of the METAFER2 system in a routine mutagenicity assay |
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