shRNA against PTEN promotes neurite outgrowth of cortical neurons and functional recovery in spinal cord contusion rats
To explore neurite growth/regeneration and spinal cord injury repair after PTEN silencing via lentivirus-mediated RNAi. Cortical neurons were seeded on or adjacent to chondroitin sulfate proteoglycans. The length, number and crossing behavior of neurites were calculated. Lentivirus was locally injec...
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| Veröffentlicht in: | Regenerative medicine 2015-05, Vol.10 (4), p.411-429 |
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| container_title | Regenerative medicine |
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| creator | Zhou, Hengxing Li, Xueying Wu, Qiang Li, Fuyuan Fu, Zheng Liu, Chang Liang, Zhipin Chu, Tianci Wang, Tianyi Lu, Lu Ning, Guangzhi Kong, Xiaohong Feng, Shiqing |
| description | To explore neurite growth/regeneration and spinal cord injury repair after PTEN silencing via lentivirus-mediated RNAi.
Cortical neurons were seeded on or adjacent to chondroitin sulfate proteoglycans. The length, number and crossing behavior of neurites were calculated. Lentivirus was locally injected into spinal cord contusion rats. The functional recovery and immunohistochemical staining were analyzed.
Neurites with PTEN silencing exhibited significant enhancements in elongation, initiation and crossing ability when they encountered chondroitin sulfate proteoglycans in vitro. In vivo PTEN silencing improved functional recovery significantly, and promoted axon and synapse formation, but not scar formation.
PTEN silencing may be promising for spinal cord injury repair. |
| doi_str_mv | 10.2217/rme.14.88 |
| format | Article |
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Cortical neurons were seeded on or adjacent to chondroitin sulfate proteoglycans. The length, number and crossing behavior of neurites were calculated. Lentivirus was locally injected into spinal cord contusion rats. The functional recovery and immunohistochemical staining were analyzed.
Neurites with PTEN silencing exhibited significant enhancements in elongation, initiation and crossing ability when they encountered chondroitin sulfate proteoglycans in vitro. In vivo PTEN silencing improved functional recovery significantly, and promoted axon and synapse formation, but not scar formation.
PTEN silencing may be promising for spinal cord injury repair.</description><identifier>ISSN: 1746-0751</identifier><identifier>EISSN: 1746-076X</identifier><identifier>DOI: 10.2217/rme.14.88</identifier><identifier>PMID: 25495396</identifier><language>eng</language><publisher>England: Future Medicine Ltd</publisher><subject>Analysis ; Animals ; Axons - pathology ; Care and treatment ; Cell proliferation ; Cells, Cultured ; Cerebral Cortex - pathology ; Cicatrix - pathology ; Gene Knockdown Techniques ; Gene Silencing ; Hindlimb - pathology ; Hindlimb - physiopathology ; Intervention (Psychology) ; Lentivirus - metabolism ; Motor Activity ; Neurites - metabolism ; Neurites - pathology ; Plasmids - metabolism ; PTEN Phosphohydrolase - metabolism ; Rats, Wistar ; Recovery of Function ; RNA interference ; RNA, Small Interfering - metabolism ; Spinal cord injuries ; Spinal Cord Injuries - pathology ; Spinal Cord Injuries - physiopathology ; Synapses - metabolism</subject><ispartof>Regenerative medicine, 2015-05, Vol.10 (4), p.411-429</ispartof><rights>COPYRIGHT 2015 Future Medicine Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c352t-f4b9c228128dc73e1926f7bbf9a4cf26653bc14f815a697569b94c3a291388e73</citedby><cites>FETCH-LOGICAL-c352t-f4b9c228128dc73e1926f7bbf9a4cf26653bc14f815a697569b94c3a291388e73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,27929,27930</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25495396$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhou, Hengxing</creatorcontrib><creatorcontrib>Li, Xueying</creatorcontrib><creatorcontrib>Wu, Qiang</creatorcontrib><creatorcontrib>Li, Fuyuan</creatorcontrib><creatorcontrib>Fu, Zheng</creatorcontrib><creatorcontrib>Liu, Chang</creatorcontrib><creatorcontrib>Liang, Zhipin</creatorcontrib><creatorcontrib>Chu, Tianci</creatorcontrib><creatorcontrib>Wang, Tianyi</creatorcontrib><creatorcontrib>Lu, Lu</creatorcontrib><creatorcontrib>Ning, Guangzhi</creatorcontrib><creatorcontrib>Kong, Xiaohong</creatorcontrib><creatorcontrib>Feng, Shiqing</creatorcontrib><title>shRNA against PTEN promotes neurite outgrowth of cortical neurons and functional recovery in spinal cord contusion rats</title><title>Regenerative medicine</title><addtitle>Regen Med</addtitle><description>To explore neurite growth/regeneration and spinal cord injury repair after PTEN silencing via lentivirus-mediated RNAi.
Cortical neurons were seeded on or adjacent to chondroitin sulfate proteoglycans. The length, number and crossing behavior of neurites were calculated. Lentivirus was locally injected into spinal cord contusion rats. The functional recovery and immunohistochemical staining were analyzed.
Neurites with PTEN silencing exhibited significant enhancements in elongation, initiation and crossing ability when they encountered chondroitin sulfate proteoglycans in vitro. In vivo PTEN silencing improved functional recovery significantly, and promoted axon and synapse formation, but not scar formation.
PTEN silencing may be promising for spinal cord injury repair.</description><subject>Analysis</subject><subject>Animals</subject><subject>Axons - pathology</subject><subject>Care and treatment</subject><subject>Cell proliferation</subject><subject>Cells, Cultured</subject><subject>Cerebral Cortex - pathology</subject><subject>Cicatrix - pathology</subject><subject>Gene Knockdown Techniques</subject><subject>Gene Silencing</subject><subject>Hindlimb - pathology</subject><subject>Hindlimb - physiopathology</subject><subject>Intervention (Psychology)</subject><subject>Lentivirus - metabolism</subject><subject>Motor Activity</subject><subject>Neurites - metabolism</subject><subject>Neurites - pathology</subject><subject>Plasmids - metabolism</subject><subject>PTEN Phosphohydrolase - metabolism</subject><subject>Rats, Wistar</subject><subject>Recovery of Function</subject><subject>RNA interference</subject><subject>RNA, Small Interfering - metabolism</subject><subject>Spinal cord injuries</subject><subject>Spinal Cord Injuries - pathology</subject><subject>Spinal Cord Injuries - physiopathology</subject><subject>Synapses - metabolism</subject><issn>1746-0751</issn><issn>1746-076X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkV1rFDEUhoMotlYv_AMS8EYvdjv5nORyKfUDSi1SwbuQyZxsIzPJmmQs_ffNurUglMBJeM_zHnJ4EXpLujWlpD_NM6wJXyv1DB2TnstV18ufzx_fghyhV6X86jrBlKAv0REVXAum5TG6LTffLzfYbm2IpeKr6_NLvMtpThUKjrDkUAGnpW5zuq03OHnsUq7B2elvN8WCbRyxX6KrIcUmZ3DpD-Q7HCIuu7CXmmVsJdalNAZnW8tr9MLbqcCbh_sE_fh0fn32ZXXx7fPXs83FyjFB68rzQTtKFaFqdD0Doqn0_TB4bbnzVErBBke4V0RYqXsh9aC5Y5ZqwpSCnp2gD4e5banfC5Rq5lAcTJONkJZiiFScM8mEbuj7A7q1E5gQfarZuj1uNpwITjrWdY1aP0G1M8Ic2o7gQ9P_M3w8GFxOpWTwZpfDbPOdIZ3Zx2dafIZwo1Rj3z38dhlmGB_Jf3mxe3VHlaw</recordid><startdate>20150501</startdate><enddate>20150501</enddate><creator>Zhou, Hengxing</creator><creator>Li, Xueying</creator><creator>Wu, Qiang</creator><creator>Li, Fuyuan</creator><creator>Fu, Zheng</creator><creator>Liu, Chang</creator><creator>Liang, Zhipin</creator><creator>Chu, Tianci</creator><creator>Wang, Tianyi</creator><creator>Lu, Lu</creator><creator>Ning, Guangzhi</creator><creator>Kong, Xiaohong</creator><creator>Feng, Shiqing</creator><general>Future Medicine Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20150501</creationdate><title>shRNA against PTEN promotes neurite outgrowth of cortical neurons and functional recovery in spinal cord contusion rats</title><author>Zhou, Hengxing ; Li, Xueying ; Wu, Qiang ; Li, Fuyuan ; Fu, Zheng ; Liu, Chang ; Liang, Zhipin ; Chu, Tianci ; Wang, Tianyi ; Lu, Lu ; Ning, Guangzhi ; Kong, Xiaohong ; Feng, Shiqing</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c352t-f4b9c228128dc73e1926f7bbf9a4cf26653bc14f815a697569b94c3a291388e73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Analysis</topic><topic>Animals</topic><topic>Axons - pathology</topic><topic>Care and treatment</topic><topic>Cell proliferation</topic><topic>Cells, Cultured</topic><topic>Cerebral Cortex - pathology</topic><topic>Cicatrix - pathology</topic><topic>Gene Knockdown Techniques</topic><topic>Gene Silencing</topic><topic>Hindlimb - pathology</topic><topic>Hindlimb - physiopathology</topic><topic>Intervention (Psychology)</topic><topic>Lentivirus - metabolism</topic><topic>Motor Activity</topic><topic>Neurites - metabolism</topic><topic>Neurites - pathology</topic><topic>Plasmids - metabolism</topic><topic>PTEN Phosphohydrolase - metabolism</topic><topic>Rats, Wistar</topic><topic>Recovery of Function</topic><topic>RNA interference</topic><topic>RNA, Small Interfering - metabolism</topic><topic>Spinal cord injuries</topic><topic>Spinal Cord Injuries - pathology</topic><topic>Spinal Cord Injuries - physiopathology</topic><topic>Synapses - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhou, Hengxing</creatorcontrib><creatorcontrib>Li, Xueying</creatorcontrib><creatorcontrib>Wu, Qiang</creatorcontrib><creatorcontrib>Li, Fuyuan</creatorcontrib><creatorcontrib>Fu, Zheng</creatorcontrib><creatorcontrib>Liu, Chang</creatorcontrib><creatorcontrib>Liang, Zhipin</creatorcontrib><creatorcontrib>Chu, Tianci</creatorcontrib><creatorcontrib>Wang, Tianyi</creatorcontrib><creatorcontrib>Lu, Lu</creatorcontrib><creatorcontrib>Ning, Guangzhi</creatorcontrib><creatorcontrib>Kong, Xiaohong</creatorcontrib><creatorcontrib>Feng, Shiqing</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Regenerative medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhou, Hengxing</au><au>Li, Xueying</au><au>Wu, Qiang</au><au>Li, Fuyuan</au><au>Fu, Zheng</au><au>Liu, Chang</au><au>Liang, Zhipin</au><au>Chu, Tianci</au><au>Wang, Tianyi</au><au>Lu, Lu</au><au>Ning, Guangzhi</au><au>Kong, Xiaohong</au><au>Feng, Shiqing</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>shRNA against PTEN promotes neurite outgrowth of cortical neurons and functional recovery in spinal cord contusion rats</atitle><jtitle>Regenerative medicine</jtitle><addtitle>Regen Med</addtitle><date>2015-05-01</date><risdate>2015</risdate><volume>10</volume><issue>4</issue><spage>411</spage><epage>429</epage><pages>411-429</pages><issn>1746-0751</issn><eissn>1746-076X</eissn><abstract>To explore neurite growth/regeneration and spinal cord injury repair after PTEN silencing via lentivirus-mediated RNAi.
Cortical neurons were seeded on or adjacent to chondroitin sulfate proteoglycans. The length, number and crossing behavior of neurites were calculated. Lentivirus was locally injected into spinal cord contusion rats. The functional recovery and immunohistochemical staining were analyzed.
Neurites with PTEN silencing exhibited significant enhancements in elongation, initiation and crossing ability when they encountered chondroitin sulfate proteoglycans in vitro. In vivo PTEN silencing improved functional recovery significantly, and promoted axon and synapse formation, but not scar formation.
PTEN silencing may be promising for spinal cord injury repair.</abstract><cop>England</cop><pub>Future Medicine Ltd</pub><pmid>25495396</pmid><doi>10.2217/rme.14.88</doi><tpages>19</tpages></addata></record> |
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| subjects | Analysis Animals Axons - pathology Care and treatment Cell proliferation Cells, Cultured Cerebral Cortex - pathology Cicatrix - pathology Gene Knockdown Techniques Gene Silencing Hindlimb - pathology Hindlimb - physiopathology Intervention (Psychology) Lentivirus - metabolism Motor Activity Neurites - metabolism Neurites - pathology Plasmids - metabolism PTEN Phosphohydrolase - metabolism Rats, Wistar Recovery of Function RNA interference RNA, Small Interfering - metabolism Spinal cord injuries Spinal Cord Injuries - pathology Spinal Cord Injuries - physiopathology Synapses - metabolism |
| title | shRNA against PTEN promotes neurite outgrowth of cortical neurons and functional recovery in spinal cord contusion rats |
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