DNA biosensor-based on fluorescence detection of E. coli O157:H7 by Au@Ag nanorods

A novel DNA sensor for the detection of the Escherichia coli O157:H7 (E. coli O157:H7) eaeA gene was constructed using surface enhanced fluorescence (SEF). The spacing distance dependence nature of Au@Ag nanorods surface enhanced fluorescence was investigated when the cy3-labled single strand DNA(ss...

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Veröffentlicht in:	Biosensors & bioelectronics 2015-08, Vol.70, p.239-245
Hauptverfasser:	Sun, Jiadi, Ji, Jian, Sun, Yanqing, Abdalhai, Mandour H., Zhang, Yinzhi, Sun, Xiulan
Format:	Artikel
Sprache:	eng
Schlagworte:	Adhesins, Bacterial - analysis Adhesins, Bacterial - genetics Au@Ag nanorods DNA Probes - chemistry DNA Probes - genetics DNA, Bacterial - analysis DNA, Bacterial - chemistry DNA, Bacterial - genetics Escherichia coli O157 - chemistry Escherichia coli O157 - genetics Escherichia coli O157 - isolation & purification Escherichia coli O157:H7 Escherichia coli Proteins - analysis Escherichia coli Proteins - genetics Gold - chemistry Metal Nanoparticles - chemistry Metal Nanoparticles - ultrastructure Nanotubes - chemistry Nanotubes - ultrastructure Reproducibility of Results Sensitivity and Specificity Silver - chemistry Spectrometry, Fluorescence - methods Surface enhanced fluorescence DNAsensor
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