The Effect of Defective Interfering RNAs on the Accumulation of Tomato Bushy Stunt Virus Proteins and Implications for Disease Attenuation
Tombusviruses, of which tomato bushy stunt virus (TBSV) is the type member, spontaneously generate defective interfering RNAs (DIs) that are known to interfere with vital accumulation and symptom development. We show that co-infection with TBSV and Dis causes a dramatic reduction in accumulation of...
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| Veröffentlicht in: | Virology (New York, N.Y.) N.Y.), 1995-08, Vol.211 (1), p.324-328 |
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| container_title | Virology (New York, N.Y.) |
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| creator | Scholthof, Karen-Beth G. Scholthof, Herman B. Jackson, Andrew O. |
| description | Tombusviruses, of which tomato bushy stunt virus (TBSV) is the type member, spontaneously generate defective interfering RNAs (DIs) that are known to interfere with vital accumulation and symptom development. We show that co-infection with TBSV and Dis causes a dramatic reduction in accumulation of TBSV subgenomic RNAs and corresponding TBSV proteins with a less dramatic reduced accumulation of the genomic RNA and the replicase proteins. Associated with this differentially regulated suppression was a greatly reduced expression of both the p19 protein, which is responsible for severe symptoms, and the p22 protein, which is associated with cell-to-cell movement of the virus. Therefore, the results suggest that the protective effect of DIs may be due to selective inhibition of p19 and p22 expression in addition to reduced replication of genomic RNA. |
| doi_str_mv | 10.1006/viro.1995.1410 |
| format | Article |
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We show that co-infection with TBSV and Dis causes a dramatic reduction in accumulation of TBSV subgenomic RNAs and corresponding TBSV proteins with a less dramatic reduced accumulation of the genomic RNA and the replicase proteins. Associated with this differentially regulated suppression was a greatly reduced expression of both the p19 protein, which is responsible for severe symptoms, and the p22 protein, which is associated with cell-to-cell movement of the virus. 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Therefore, the results suggest that the protective effect of DIs may be due to selective inhibition of p19 and p22 expression in addition to reduced replication of genomic RNA.</description><subject>ARN</subject><subject>Genome, Viral</subject><subject>Nicotiana</subject><subject>Nicotiana benthamiana</subject><subject>Open Reading Frames</subject><subject>Plant Diseases</subject><subject>Plant Leaves</subject><subject>Plants, Toxic</subject><subject>PROTEINAS</subject><subject>PROTEINE</subject><subject>Protoplasts</subject><subject>RNA, Viral - analysis</subject><subject>RNA, Viral - biosynthesis</subject><subject>SINTOMAS</subject><subject>SYMPTOME</subject><subject>tomato bushy stunt virus</subject><subject>TOMBUSVIRUS</subject><subject>Tombusvirus - genetics</subject><subject>Tombusvirus - metabolism</subject><subject>Tombusvirus - pathogenicity</subject><subject>Transcription, Genetic</subject><subject>VIRUS</subject><subject>VIRUS DE LAS PLANTAS</subject><subject>VIRUS DES VEGETAUX</subject><issn>0042-6822</issn><issn>1096-0341</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kUtv1DAUhS0EKkNhywIJySt2GezYceLl0BYYqQJEp2wtx7lujZJ46sdI_Qv8apzOiB0rP75zzpWPEXpLyZoSIj4eXPBrKmWzppySZ2hFiRQVYZw-RytCeF2Jrq5folcx_ibl3LbkDJ21gjc1Iyv0Z3cP-MpaMAl7iy9h2bkD4O2cIFgIbr7DP79tIvYzTkW7MSZPedTJlYvi2PlJJ48_5Xj_iG9SnhP-5UKO-EfwCdwcsZ4HvJ32ozNPpoitD_jSRdCxxKUEc34Cr9ELq8cIb07rObr9fLW7-Fpdf_-yvdhcV4bXMlWs5p1gDQFudKstk5yKmvUtb4H1knMz6F5TxqEdur6WwhDRWTvUvTVUDqJl5-jDMXcf_EOGmNTkooFx1DP4HBUVHWed7IpwfRSa4GMMYNU-uEmHR0WJWspXS_lqKV8t5RfD-1Ny7icY_slPbRf-7sit9krfBRfV7Y1sGtmSpsDuCKG8_eAgqGgczAYGF8qfqMG7_839C0RynXU</recordid><startdate>19950801</startdate><enddate>19950801</enddate><creator>Scholthof, Karen-Beth G.</creator><creator>Scholthof, Herman B.</creator><creator>Jackson, Andrew O.</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope></search><sort><creationdate>19950801</creationdate><title>The Effect of Defective Interfering RNAs on the Accumulation of Tomato Bushy Stunt Virus Proteins and Implications for Disease Attenuation</title><author>Scholthof, Karen-Beth G. ; 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| identifier | ISSN: 0042-6822 |
| ispartof | Virology (New York, N.Y.), 1995-08, Vol.211 (1), p.324-328 |
| issn | 0042-6822 1096-0341 |
| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier); EZB-FREE-00999 freely available EZB journals |
| subjects | ARN Genome, Viral Nicotiana Nicotiana benthamiana Open Reading Frames Plant Diseases Plant Leaves Plants, Toxic PROTEINAS PROTEINE Protoplasts RNA, Viral - analysis RNA, Viral - biosynthesis SINTOMAS SYMPTOME tomato bushy stunt virus TOMBUSVIRUS Tombusvirus - genetics Tombusvirus - metabolism Tombusvirus - pathogenicity Transcription, Genetic VIRUS VIRUS DE LAS PLANTAS VIRUS DES VEGETAUX |
| title | The Effect of Defective Interfering RNAs on the Accumulation of Tomato Bushy Stunt Virus Proteins and Implications for Disease Attenuation |
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