Production of a Renibacterium salmoninarum hemolysin fusion protein in Escherichia coli K12

Gene rsh encoding a novel hemolysin of Renibacterium salmoninarum was fused to the lacZ gene of the pAX5+ plasmid vector to facilitate production of a 160 kDa beta -galactosidase fusion protein. The soluble fusion protein was produced cytoplasmically in Escherichia coli XL1-Blue and purified using a...

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Veröffentlicht in:	Diseases of aquatic organisms 1995, Vol.22 (2), p.153-156
Hauptverfasser:	GRAYSON, T. H, EVENDEN, A. J, GILPIN, M. L, MUNN, C. B
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biotechnology Escherichia coli Fundamental and applied biological sciences. Psychology Genetic engineering Genetic technics Marine Methods. Procedures. Technologies Modification of gene expression level Renibacterium salmoninarum
Online-Zugang:	Volltext
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