Suspended, Shrinkage-Free, Electrospun PLGA Nanofibrous Scaffold for Skin Tissue Engineering
Electrospinning is a technique for creating continuous nanofibrous networks that can architecturally be similar to the structure of extracellular matrix (ECM). However, the shrinkage of electrospun mats is unfavorable for the triggering of cell adhesion and further growth. In this work, electrospun...
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| Veröffentlicht in: | ACS applied materials & interfaces 2015-05, Vol.7 (20), p.10872-10877 |
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| container_title | ACS applied materials & interfaces |
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| creator | Ru, Changhai Wang, Feilong Pang, Ming Sun, Lining Chen, Ruihua Sun, Yu |
| description | Electrospinning is a technique for creating continuous nanofibrous networks that can architecturally be similar to the structure of extracellular matrix (ECM). However, the shrinkage of electrospun mats is unfavorable for the triggering of cell adhesion and further growth. In this work, electrospun PLGA nanofiber assemblies are utilized to create a scaffold. Aided by a polypropylene auxiliary supporter, the scaffold is able to maintain long-term integrity without dimensional shrinkage. This scaffold is also able to suspend in cell culture medium; hence, keratinocyte cells seeded on the scaffold are exposed to air as required in skin tissue engineering. Experiments also show that human skin keratinocytes can proliferate on the scaffold and infiltrate into the scaffold. |
| doi_str_mv | 10.1021/acsami.5b01953 |
| format | Article |
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Experiments also show that human skin keratinocytes can proliferate on the scaffold and infiltrate into the scaffold.</description><identifier>ISSN: 1944-8244</identifier><identifier>EISSN: 1944-8252</identifier><identifier>DOI: 10.1021/acsami.5b01953</identifier><identifier>PMID: 25941905</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Bandages ; Cell Proliferation - physiology ; Cells, Cultured ; Electroplating - methods ; Equipment Design ; Equipment Failure Analysis ; Humans ; Keratinocytes - cytology ; Keratinocytes - physiology ; Lactic Acid - chemistry ; Male ; Materials Testing ; Nanofibers - chemistry ; Nanofibers - ultrastructure ; Particle Size ; Polyglycolic Acid - chemistry ; Rotation ; Skin, Artificial ; Tissue Engineering - instrumentation ; Tissue Scaffolds</subject><ispartof>ACS applied materials & interfaces, 2015-05, Vol.7 (20), p.10872-10877</ispartof><rights>Copyright © 2015 American Chemical Society</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a396t-c00fb256ff1288e14b3f719a43bf518a8a8684a9c459b39e6b062e78043b80873</citedby><cites>FETCH-LOGICAL-a396t-c00fb256ff1288e14b3f719a43bf518a8a8684a9c459b39e6b062e78043b80873</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acsami.5b01953$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acsami.5b01953$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,780,784,2763,27075,27923,27924,56737,56787</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25941905$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ru, Changhai</creatorcontrib><creatorcontrib>Wang, Feilong</creatorcontrib><creatorcontrib>Pang, Ming</creatorcontrib><creatorcontrib>Sun, Lining</creatorcontrib><creatorcontrib>Chen, Ruihua</creatorcontrib><creatorcontrib>Sun, Yu</creatorcontrib><title>Suspended, Shrinkage-Free, Electrospun PLGA Nanofibrous Scaffold for Skin Tissue Engineering</title><title>ACS applied materials & interfaces</title><addtitle>ACS Appl. Mater. Interfaces</addtitle><description>Electrospinning is a technique for creating continuous nanofibrous networks that can architecturally be similar to the structure of extracellular matrix (ECM). However, the shrinkage of electrospun mats is unfavorable for the triggering of cell adhesion and further growth. In this work, electrospun PLGA nanofiber assemblies are utilized to create a scaffold. Aided by a polypropylene auxiliary supporter, the scaffold is able to maintain long-term integrity without dimensional shrinkage. This scaffold is also able to suspend in cell culture medium; hence, keratinocyte cells seeded on the scaffold are exposed to air as required in skin tissue engineering. Experiments also show that human skin keratinocytes can proliferate on the scaffold and infiltrate into the scaffold.</description><subject>Bandages</subject><subject>Cell Proliferation - physiology</subject><subject>Cells, Cultured</subject><subject>Electroplating - methods</subject><subject>Equipment Design</subject><subject>Equipment Failure Analysis</subject><subject>Humans</subject><subject>Keratinocytes - cytology</subject><subject>Keratinocytes - physiology</subject><subject>Lactic Acid - chemistry</subject><subject>Male</subject><subject>Materials Testing</subject><subject>Nanofibers - chemistry</subject><subject>Nanofibers - ultrastructure</subject><subject>Particle Size</subject><subject>Polyglycolic Acid - chemistry</subject><subject>Rotation</subject><subject>Skin, Artificial</subject><subject>Tissue Engineering - instrumentation</subject><subject>Tissue Scaffolds</subject><issn>1944-8244</issn><issn>1944-8252</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kDFPwzAQRi0EoqWwMiKPCDXFTuzEHquqLUgVIKVsSJGTnEvaxCl2M_DvMUrphm64G959unsI3VIyoSSkj6pwqqkmPCdU8ugMDalkLBAhD89PM2MDdOXclpA4Cgm_RIOQS0Yl4UP0kXZuD6aEcozTT1uZndpAsLAAYzyvoTjY1u07g99Wyyl-UabVVW7bzuG0UFq3dYl1a3G6qwxeV851gOdmUxkAH7W5Rhda1Q5ujn2E3hfz9ewpWL0un2fTVaAiGR-CghCdhzzWmoZCAGV5pBMqFYtyzalQvmLBlCwYl3kkIc5JHEIiiAcEEUk0Qvd97t62Xx24Q9ZUroC6Vgb8rRmNRZRwTryhEZr0aOEfcxZ0trdVo-x3Rkn2azTrjWZHo37h7pjd5Q2UJ_xPoQceesAvZtu2s8a_-l_aDx8Nf64</recordid><startdate>20150527</startdate><enddate>20150527</enddate><creator>Ru, Changhai</creator><creator>Wang, Feilong</creator><creator>Pang, Ming</creator><creator>Sun, Lining</creator><creator>Chen, Ruihua</creator><creator>Sun, Yu</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20150527</creationdate><title>Suspended, Shrinkage-Free, Electrospun PLGA Nanofibrous Scaffold for Skin Tissue Engineering</title><author>Ru, Changhai ; Wang, Feilong ; Pang, Ming ; Sun, Lining ; Chen, Ruihua ; Sun, Yu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a396t-c00fb256ff1288e14b3f719a43bf518a8a8684a9c459b39e6b062e78043b80873</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Bandages</topic><topic>Cell Proliferation - physiology</topic><topic>Cells, Cultured</topic><topic>Electroplating - methods</topic><topic>Equipment Design</topic><topic>Equipment Failure Analysis</topic><topic>Humans</topic><topic>Keratinocytes - cytology</topic><topic>Keratinocytes - physiology</topic><topic>Lactic Acid - chemistry</topic><topic>Male</topic><topic>Materials Testing</topic><topic>Nanofibers - chemistry</topic><topic>Nanofibers - ultrastructure</topic><topic>Particle Size</topic><topic>Polyglycolic Acid - chemistry</topic><topic>Rotation</topic><topic>Skin, Artificial</topic><topic>Tissue Engineering - instrumentation</topic><topic>Tissue Scaffolds</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ru, Changhai</creatorcontrib><creatorcontrib>Wang, Feilong</creatorcontrib><creatorcontrib>Pang, Ming</creatorcontrib><creatorcontrib>Sun, Lining</creatorcontrib><creatorcontrib>Chen, Ruihua</creatorcontrib><creatorcontrib>Sun, Yu</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>ACS applied materials & interfaces</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ru, Changhai</au><au>Wang, Feilong</au><au>Pang, Ming</au><au>Sun, Lining</au><au>Chen, Ruihua</au><au>Sun, Yu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Suspended, Shrinkage-Free, Electrospun PLGA Nanofibrous Scaffold for Skin Tissue Engineering</atitle><jtitle>ACS applied materials & interfaces</jtitle><addtitle>ACS Appl. Mater. Interfaces</addtitle><date>2015-05-27</date><risdate>2015</risdate><volume>7</volume><issue>20</issue><spage>10872</spage><epage>10877</epage><pages>10872-10877</pages><issn>1944-8244</issn><eissn>1944-8252</eissn><abstract>Electrospinning is a technique for creating continuous nanofibrous networks that can architecturally be similar to the structure of extracellular matrix (ECM). However, the shrinkage of electrospun mats is unfavorable for the triggering of cell adhesion and further growth. In this work, electrospun PLGA nanofiber assemblies are utilized to create a scaffold. Aided by a polypropylene auxiliary supporter, the scaffold is able to maintain long-term integrity without dimensional shrinkage. This scaffold is also able to suspend in cell culture medium; hence, keratinocyte cells seeded on the scaffold are exposed to air as required in skin tissue engineering. 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| subjects | Bandages Cell Proliferation - physiology Cells, Cultured Electroplating - methods Equipment Design Equipment Failure Analysis Humans Keratinocytes - cytology Keratinocytes - physiology Lactic Acid - chemistry Male Materials Testing Nanofibers - chemistry Nanofibers - ultrastructure Particle Size Polyglycolic Acid - chemistry Rotation Skin, Artificial Tissue Engineering - instrumentation Tissue Scaffolds |
| title | Suspended, Shrinkage-Free, Electrospun PLGA Nanofibrous Scaffold for Skin Tissue Engineering |
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