Cloning and Characterization of a Putative G-Protein Coupled Receptor Potentially Involved in Development
Polymerase chain reaction techniques and medium stringency library screening were used to isolate a rat cDNA ("H218") which encodes a novel guanine nucleotide-binding protein coupled receptor homolog ("p H218"). Northern analysis revealed that brain H218 mRNA is preferentially ex...
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| Veröffentlicht in: | Molecular and cellular neuroscience 1994, Vol.5 (3), p.201-209 |
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| container_title | Molecular and cellular neuroscience |
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| creator | MacLennan, A.John Browe, Christopher S. Gaskin, Amanda A. Lado, David C. Shaw, Gerry |
| description | Polymerase chain reaction techniques and medium stringency library screening were used to isolate a rat cDNA ("H218") which encodes a novel guanine nucleotide-binding protein coupled receptor homolog ("p
H218"). Northern analysis revealed that brain H218 mRNA is preferentially expressed during embryogenesis. In addition, H218 mRNA is expressed in all developing tissues and rodent cell lines examined with highest levels detected in primitive, transformed cells. H218 mRNA expression in cell lines is rapidly increased by a tumor promoter and rapidly decreased by a differentiation-inducing growth factor. Finally, all of the sequence motifs characteristic of Src homology 2 domains are present in p
H218 but in a unique arrangement. We conclude that p
H218 may function as a growth factor receptor. |
| doi_str_mv | 10.1006/mcne.1994.1024 |
| format | Article |
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H218"). Northern analysis revealed that brain H218 mRNA is preferentially expressed during embryogenesis. In addition, H218 mRNA is expressed in all developing tissues and rodent cell lines examined with highest levels detected in primitive, transformed cells. H218 mRNA expression in cell lines is rapidly increased by a tumor promoter and rapidly decreased by a differentiation-inducing growth factor. Finally, all of the sequence motifs characteristic of Src homology 2 domains are present in p
H218 but in a unique arrangement. We conclude that p
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H218"). Northern analysis revealed that brain H218 mRNA is preferentially expressed during embryogenesis. In addition, H218 mRNA is expressed in all developing tissues and rodent cell lines examined with highest levels detected in primitive, transformed cells. H218 mRNA expression in cell lines is rapidly increased by a tumor promoter and rapidly decreased by a differentiation-inducing growth factor. Finally, all of the sequence motifs characteristic of Src homology 2 domains are present in p
H218 but in a unique arrangement. We conclude that p
H218 may function as a growth factor receptor.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Cloning, Molecular</subject><subject>Cricetinae</subject><subject>DNA, Complementary - genetics</subject><subject>Fibroblasts - metabolism</subject><subject>Gene Expression Regulation - drug effects</subject><subject>GTP-Binding Proteins</subject><subject>Mice</subject><subject>Molecular Sequence Data</subject><subject>PC12 Cells - drug effects</subject><subject>Polymerase Chain Reaction</subject><subject>Protein Structure, Tertiary</subject><subject>Rats</subject><subject>Receptors, Cell Surface - biosynthesis</subject><subject>Receptors, Cell Surface - chemistry</subject><subject>Receptors, Cell Surface - genetics</subject><subject>Receptors, Cell Surface - physiology</subject><subject>Receptors, Dopamine - genetics</subject><subject>Receptors, G-Protein-Coupled</subject><subject>Receptors, Lysophospholipid</subject><subject>RNA, Messenger - biosynthesis</subject><subject>Sequence Alignment</subject><subject>Sequence Homology, Amino Acid</subject><subject>Species Specificity</subject><subject>Tetradecanoylphorbol Acetate - pharmacology</subject><subject>Tumor Cells, Cultured</subject><issn>1044-7431</issn><issn>1095-9327</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kEtr3DAUhUVpybPb7gJadeeJXrblZXHbNBDIUJK10MjXrYosuZJsSH59ZWboLqv7OOceuB9CnyjZUUKa28l42NGuE2Vk4h26oKSrq46z9v3WC1G1gtNzdJnSH0JIzTp-hs4kka2g8gLZ3gVv_S-s_YD73zpqkyHaV51t8DiMWOP9ksu0Ar6r9jFksB73YZkdDPgnGJhziHhf9j5b7dwLvvdrcGtRi_ErrODCPBXxGn0YtUvw8VSv0PP3b0_9j-rh8e6-__JQGdHUuTrUA2UNEDk2rGO0aZgmlPDyYcsl4wNnxnQwEnrghgDRhtUSqKANkaJtmeZX6PMxd47h7wIpq8kmA85pD2FJijaS81bWxbg7Gk0MKUUY1RztpOOLokRtbNXGVm1s1ca2HNyckpfDBMN_-wlm0eVRh_LeaiGqZCx4A4ONYLIagn0r-h_Hn4gh</recordid><startdate>1994</startdate><enddate>1994</enddate><creator>MacLennan, A.John</creator><creator>Browe, Christopher S.</creator><creator>Gaskin, Amanda A.</creator><creator>Lado, David C.</creator><creator>Shaw, Gerry</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope></search><sort><creationdate>1994</creationdate><title>Cloning and Characterization of a Putative G-Protein Coupled Receptor Potentially Involved in Development</title><author>MacLennan, A.John ; 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H218"). Northern analysis revealed that brain H218 mRNA is preferentially expressed during embryogenesis. In addition, H218 mRNA is expressed in all developing tissues and rodent cell lines examined with highest levels detected in primitive, transformed cells. H218 mRNA expression in cell lines is rapidly increased by a tumor promoter and rapidly decreased by a differentiation-inducing growth factor. Finally, all of the sequence motifs characteristic of Src homology 2 domains are present in p
H218 but in a unique arrangement. We conclude that p
H218 may function as a growth factor receptor.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>8087418</pmid><doi>10.1006/mcne.1994.1024</doi><tpages>9</tpages></addata></record> |
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| ispartof | Molecular and cellular neuroscience, 1994, Vol.5 (3), p.201-209 |
| issn | 1044-7431 1095-9327 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Amino Acid Sequence Animals Base Sequence Cloning, Molecular Cricetinae DNA, Complementary - genetics Fibroblasts - metabolism Gene Expression Regulation - drug effects GTP-Binding Proteins Mice Molecular Sequence Data PC12 Cells - drug effects Polymerase Chain Reaction Protein Structure, Tertiary Rats Receptors, Cell Surface - biosynthesis Receptors, Cell Surface - chemistry Receptors, Cell Surface - genetics Receptors, Cell Surface - physiology Receptors, Dopamine - genetics Receptors, G-Protein-Coupled Receptors, Lysophospholipid RNA, Messenger - biosynthesis Sequence Alignment Sequence Homology, Amino Acid Species Specificity Tetradecanoylphorbol Acetate - pharmacology Tumor Cells, Cultured |
| title | Cloning and Characterization of a Putative G-Protein Coupled Receptor Potentially Involved in Development |
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