Sensitive fluorescent assay for copper (II) determination in aqueous solution using copper-specific ssDNA and Sybr Green I
This paper reports a fluorescent turn-off assay for sensitive detection of Cu2+ in an aqueous solution by using a copper-specific ssDNA Cu100 and Sybr Green I. By monitoring the fluorescence changes arose from different interactions of Sybr Green I with Cu100 and Cu100/Cu2+ complex, the Cu2+ could b...
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| Veröffentlicht in: | Talanta (Oxford) 2015-09, Vol.142, p.176-182 |
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| creator | Zhan, Shenshan Xu, Hanchu Zhang, Weilin Zhan, Xuejia Wu, Yuangen Wang, Lumei Zhou, Pei |
| description | This paper reports a fluorescent turn-off assay for sensitive detection of Cu2+ in an aqueous solution by using a copper-specific ssDNA Cu100 and Sybr Green I. By monitoring the fluorescence changes arose from different interactions of Sybr Green I with Cu100 and Cu100/Cu2+ complex, the Cu2+ could be linearly detected from 5.57 to 250ppb, with a detection limit of 5.57ppb. The feasibility of this assay was demonstrated by detecting Cu2+ in certified reference materials and spiked water samples with satisfactory results.
[Display omitted]
•A copper-specific ssDNA named Cu100 was firstly proposed.•The recognition ability of Cu100 to Cu2+ was preliminary verified.•A fluorescent assay for Cu2+ detection was designed based on Cu100 and Sybr Green I.•This assay provides a linear range of 5.57–250ppb and a detection limit of 5.57ppb.•It is hoped that the Cu100 can be applied as an alternative of the Cu2+-dependent DNAzyme. |
| doi_str_mv | 10.1016/j.talanta.2015.04.037 |
| format | Article |
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[Display omitted]
•A copper-specific ssDNA named Cu100 was firstly proposed.•The recognition ability of Cu100 to Cu2+ was preliminary verified.•A fluorescent assay for Cu2+ detection was designed based on Cu100 and Sybr Green I.•This assay provides a linear range of 5.57–250ppb and a detection limit of 5.57ppb.•It is hoped that the Cu100 can be applied as an alternative of the Cu2+-dependent DNAzyme.</description><identifier>ISSN: 0039-9140</identifier><identifier>EISSN: 1873-3573</identifier><identifier>DOI: 10.1016/j.talanta.2015.04.037</identifier><identifier>PMID: 26003709</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Copper (II) determination ; Copper - analysis ; Copper - chemistry ; Copper-specific ssDNA ; DNA, Single-Stranded - chemistry ; Fluorescent assay ; Fluorescent Dyes - chemistry ; Organic Chemicals - chemistry ; Solutions ; Spectrometry, Fluorescence ; Sybr Green I ; Water Pollutants, Chemical - analysis ; Water Pollutants, Chemical - chemistry</subject><ispartof>Talanta (Oxford), 2015-09, Vol.142, p.176-182</ispartof><rights>2015 Elsevier B.V.</rights><rights>Copyright © 2015 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c435t-c41232b6c27e22440afa9cd9dfe6909431636653f84ec32ab6bd7a53aef43d2e3</citedby><cites>FETCH-LOGICAL-c435t-c41232b6c27e22440afa9cd9dfe6909431636653f84ec32ab6bd7a53aef43d2e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.talanta.2015.04.037$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26003709$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhan, Shenshan</creatorcontrib><creatorcontrib>Xu, Hanchu</creatorcontrib><creatorcontrib>Zhang, Weilin</creatorcontrib><creatorcontrib>Zhan, Xuejia</creatorcontrib><creatorcontrib>Wu, Yuangen</creatorcontrib><creatorcontrib>Wang, Lumei</creatorcontrib><creatorcontrib>Zhou, Pei</creatorcontrib><title>Sensitive fluorescent assay for copper (II) determination in aqueous solution using copper-specific ssDNA and Sybr Green I</title><title>Talanta (Oxford)</title><addtitle>Talanta</addtitle><description>This paper reports a fluorescent turn-off assay for sensitive detection of Cu2+ in an aqueous solution by using a copper-specific ssDNA Cu100 and Sybr Green I. By monitoring the fluorescence changes arose from different interactions of Sybr Green I with Cu100 and Cu100/Cu2+ complex, the Cu2+ could be linearly detected from 5.57 to 250ppb, with a detection limit of 5.57ppb. The feasibility of this assay was demonstrated by detecting Cu2+ in certified reference materials and spiked water samples with satisfactory results.
[Display omitted]
•A copper-specific ssDNA named Cu100 was firstly proposed.•The recognition ability of Cu100 to Cu2+ was preliminary verified.•A fluorescent assay for Cu2+ detection was designed based on Cu100 and Sybr Green I.•This assay provides a linear range of 5.57–250ppb and a detection limit of 5.57ppb.•It is hoped that the Cu100 can be applied as an alternative of the Cu2+-dependent DNAzyme.</description><subject>Copper (II) determination</subject><subject>Copper - analysis</subject><subject>Copper - chemistry</subject><subject>Copper-specific ssDNA</subject><subject>DNA, Single-Stranded - chemistry</subject><subject>Fluorescent assay</subject><subject>Fluorescent Dyes - chemistry</subject><subject>Organic Chemicals - chemistry</subject><subject>Solutions</subject><subject>Spectrometry, Fluorescence</subject><subject>Sybr Green I</subject><subject>Water Pollutants, Chemical - analysis</subject><subject>Water Pollutants, Chemical - chemistry</subject><issn>0039-9140</issn><issn>1873-3573</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1v1DAQhi0EotvCTwD5WA4J_oqzOaGqtGWlih5azpZjj5FXWTvYTqXl1-PtLly5zEgz7zsfD0IfKGkpofLzti160qHolhHatUS0hPev0Ique97wruev0YoQPjQDFeQMnee8JYQwTvhbdMZkbfVkWKHfjxCyL_4ZsJuWmCAbCAXrnPUeu5iwifMMCV9uNp-whQJp54MuPgbsA9a_FohLxjlOy0ttyT78PHmaPIPxzhuc89fvV1gHix_3Y8J3CSDgzTv0xukpw_tTvkA_bm-err819w93m-ur-8YI3pUaKeNslIb1wJgQRDs9GDtYB3Igg-BUcik77tYCDGd6lKPtdcc1OMEtA36BLo9z5xTrvbmona9fTpXe4XhF5ZrzTvRUVGl3lJoUc07g1Jz8Tqe9okQdsKutOmFXB-yKCFVBVt_H04pl3IH95_rLuQq-HAVQH332kFQ2HoIB6xOYomz0_1nxB9KEl5g</recordid><startdate>20150901</startdate><enddate>20150901</enddate><creator>Zhan, Shenshan</creator><creator>Xu, Hanchu</creator><creator>Zhang, Weilin</creator><creator>Zhan, Xuejia</creator><creator>Wu, Yuangen</creator><creator>Wang, Lumei</creator><creator>Zhou, Pei</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20150901</creationdate><title>Sensitive fluorescent assay for copper (II) determination in aqueous solution using copper-specific ssDNA and Sybr Green I</title><author>Zhan, Shenshan ; Xu, Hanchu ; Zhang, Weilin ; Zhan, Xuejia ; Wu, Yuangen ; Wang, Lumei ; Zhou, Pei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c435t-c41232b6c27e22440afa9cd9dfe6909431636653f84ec32ab6bd7a53aef43d2e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Copper (II) determination</topic><topic>Copper - analysis</topic><topic>Copper - chemistry</topic><topic>Copper-specific ssDNA</topic><topic>DNA, Single-Stranded - chemistry</topic><topic>Fluorescent assay</topic><topic>Fluorescent Dyes - chemistry</topic><topic>Organic Chemicals - chemistry</topic><topic>Solutions</topic><topic>Spectrometry, Fluorescence</topic><topic>Sybr Green I</topic><topic>Water Pollutants, Chemical - analysis</topic><topic>Water Pollutants, Chemical - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhan, Shenshan</creatorcontrib><creatorcontrib>Xu, Hanchu</creatorcontrib><creatorcontrib>Zhang, Weilin</creatorcontrib><creatorcontrib>Zhan, Xuejia</creatorcontrib><creatorcontrib>Wu, Yuangen</creatorcontrib><creatorcontrib>Wang, Lumei</creatorcontrib><creatorcontrib>Zhou, Pei</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Talanta (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhan, Shenshan</au><au>Xu, Hanchu</au><au>Zhang, Weilin</au><au>Zhan, Xuejia</au><au>Wu, Yuangen</au><au>Wang, Lumei</au><au>Zhou, Pei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sensitive fluorescent assay for copper (II) determination in aqueous solution using copper-specific ssDNA and Sybr Green I</atitle><jtitle>Talanta (Oxford)</jtitle><addtitle>Talanta</addtitle><date>2015-09-01</date><risdate>2015</risdate><volume>142</volume><spage>176</spage><epage>182</epage><pages>176-182</pages><issn>0039-9140</issn><eissn>1873-3573</eissn><abstract>This paper reports a fluorescent turn-off assay for sensitive detection of Cu2+ in an aqueous solution by using a copper-specific ssDNA Cu100 and Sybr Green I. By monitoring the fluorescence changes arose from different interactions of Sybr Green I with Cu100 and Cu100/Cu2+ complex, the Cu2+ could be linearly detected from 5.57 to 250ppb, with a detection limit of 5.57ppb. The feasibility of this assay was demonstrated by detecting Cu2+ in certified reference materials and spiked water samples with satisfactory results.
[Display omitted]
•A copper-specific ssDNA named Cu100 was firstly proposed.•The recognition ability of Cu100 to Cu2+ was preliminary verified.•A fluorescent assay for Cu2+ detection was designed based on Cu100 and Sybr Green I.•This assay provides a linear range of 5.57–250ppb and a detection limit of 5.57ppb.•It is hoped that the Cu100 can be applied as an alternative of the Cu2+-dependent DNAzyme.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>26003709</pmid><doi>10.1016/j.talanta.2015.04.037</doi><tpages>7</tpages></addata></record> |
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| subjects | Copper (II) determination Copper - analysis Copper - chemistry Copper-specific ssDNA DNA, Single-Stranded - chemistry Fluorescent assay Fluorescent Dyes - chemistry Organic Chemicals - chemistry Solutions Spectrometry, Fluorescence Sybr Green I Water Pollutants, Chemical - analysis Water Pollutants, Chemical - chemistry |
| title | Sensitive fluorescent assay for copper (II) determination in aqueous solution using copper-specific ssDNA and Sybr Green I |
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