Protamine sulfate precipitation method depletes abundant plant seed-storage proteins: A case study on legume plants

Depletion of abundant proteins is one of the effective ways to improve detection and identification of low‐abundance proteins. Our previous study showed that protamine sulfate precipitation (PSP) method can deplete abundant ribulose‐1,5‐bisphosphate carboxylase/oxygenase (RuBisCO) from leaf proteins...

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Veröffentlicht in:	Proteomics (Weinheim) 2015-05, Vol.15 (10), p.1760-1764
Hauptverfasser:	Kim, Yu Ji, Wang, Yiming, Gupta, Ravi, Kim, So Wun, Min, Chul Woo, Kim, Yong Chul, Park, Ki Hun, Agrawal, Ganesh Kumar, Rakwal, Randeep, Choung, Myoung-Gun, Kang, Kyu Young, Kim, Sun Tae
Format:	Artikel
Sprache:	eng
Schlagworte:	Arachis - metabolism Arachis hypogaea Case studies Chemical Precipitation Coefficient of variation Comparative studies Depletion Electrophoresis, Gel, Two-Dimensional Fabaceae - metabolism Gel electrophoresis Glycine max - metabolism Legume seeds Legumes Oxygenase Peanuts Plant Proteins - metabolism Plant proteomics Principal Component Analysis Principal components analysis Protamine sulfate Protamine sulfate precipitation method Protamines - chemistry Proteins Proteomes Proteomics - methods Reproducibility Ribulose-bisphosphate carboxylase Seed proteome Seed storage proteins Seed Storage Proteins - metabolism Seeds Seeds - metabolism Sodium lauryl sulfate Soybeans Spots Storage Storage proteins Sulfates Western blotting
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