Effects of in Utero Exposure to Di(n-butyl) Phthalate for Estrogen Receptors α, β, and Androgen Receptor of Leydig Cell on Rats
Estrogens and androgens affect male and female reproductive systems. Recently, we reported that prenatal di(n-butyl) phthalate (DBP) exposure induced atypical Leydig cells (LCs) hyperplasia during adulthood. The present study investigated the expression of estrogen receptor α (ERα), estrogen recepto...
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| Veröffentlicht in: | Toxicologic pathology 2014-07, Vol.42 (5), p.877-887 |
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| creator | Wakui, Shin Shirai, Masaru Motohashi, Masaya Mutou, Tomoko Oyama, Noriko Wempe, Michael F. Takahashi, Hiroyuki Inomata, Tomoo Ikegami, Masahiro Endou, Hitoshi Asari, Masao |
| description | Estrogens and androgens affect male and female reproductive systems. Recently, we reported that prenatal di(n-butyl) phthalate (DBP) exposure induced atypical Leydig cells (LCs) hyperplasia during adulthood. The present study investigated the expression of estrogen receptor α (ERα), estrogen receptor β (ERβ), and androgen receptor (AR) in LCs of 5-, 7-, 9-, 14-, and 17-week-old Sprague-Dawley (srl) rats whose dams had been administered DBP intragastrically at 100 mg/kg/day or the vehicle (corn oil) from days 12 to 21 postconception. Immunohistochemical, Western blotting, and reverse transcription polymerase chain reaction analyses revealed that the expressions of ERα, ERβ, and AR proteins and mRNAs in the DBP group were similar to those of the vehicle group at 5 and 7 weeks, but significantly higher ERα and lower ERβ and AR levels were observed in the DBP group at 9 to 17 weeks. The rats prenatally exposed to DBP had seminiferous tubule degeneration and atypical hyperplasia of LCs during adulthood, which was associated with an increase in expression of ERα and a decrease of ERβ and AR in the testis. |
| doi_str_mv | 10.1177/0192623313502879 |
| format | Article |
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Recently, we reported that prenatal di(n-butyl) phthalate (DBP) exposure induced atypical Leydig cells (LCs) hyperplasia during adulthood. The present study investigated the expression of estrogen receptor α (ERα), estrogen receptor β (ERβ), and androgen receptor (AR) in LCs of 5-, 7-, 9-, 14-, and 17-week-old Sprague-Dawley (srl) rats whose dams had been administered DBP intragastrically at 100 mg/kg/day or the vehicle (corn oil) from days 12 to 21 postconception. Immunohistochemical, Western blotting, and reverse transcription polymerase chain reaction analyses revealed that the expressions of ERα, ERβ, and AR proteins and mRNAs in the DBP group were similar to those of the vehicle group at 5 and 7 weeks, but significantly higher ERα and lower ERβ and AR levels were observed in the DBP group at 9 to 17 weeks. The rats prenatally exposed to DBP had seminiferous tubule degeneration and atypical hyperplasia of LCs during adulthood, which was associated with an increase in expression of ERα and a decrease of ERβ and AR in the testis.</description><identifier>ISSN: 0192-6233</identifier><identifier>EISSN: 1533-1601</identifier><identifier>DOI: 10.1177/0192623313502879</identifier><identifier>PMID: 24067674</identifier><language>eng</language><publisher>Los Angeles, CA: SAGE Publications</publisher><subject>Animals ; Dibutyl Phthalate - toxicity ; Estrogen Receptor alpha - genetics ; Estrogen Receptor alpha - metabolism ; Estrogen Receptor beta - genetics ; Estrogen Receptor beta - metabolism ; Female ; Leydig Cells - drug effects ; Leydig Cells - metabolism ; Male ; Pregnancy ; Prenatal Exposure Delayed Effects - genetics ; Prenatal Exposure Delayed Effects - metabolism ; Rats ; Rats, Sprague-Dawley ; Receptors, Androgen - genetics ; Receptors, Androgen - metabolism</subject><ispartof>Toxicologic pathology, 2014-07, Vol.42 (5), p.877-887</ispartof><rights>2014 by The Author(s)</rights><rights>2014 by The Author(s).</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c370t-e519b412aab6581b407d013b3c2d045b1ab16862bcc8d436ded591de4cd276fc3</citedby><cites>FETCH-LOGICAL-c370t-e519b412aab6581b407d013b3c2d045b1ab16862bcc8d436ded591de4cd276fc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.sagepub.com/doi/pdf/10.1177/0192623313502879$$EPDF$$P50$$Gsage$$H</linktopdf><linktohtml>$$Uhttps://journals.sagepub.com/doi/10.1177/0192623313502879$$EHTML$$P50$$Gsage$$H</linktohtml><link.rule.ids>314,776,780,21798,27901,27902,43597,43598</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24067674$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wakui, Shin</creatorcontrib><creatorcontrib>Shirai, Masaru</creatorcontrib><creatorcontrib>Motohashi, Masaya</creatorcontrib><creatorcontrib>Mutou, Tomoko</creatorcontrib><creatorcontrib>Oyama, Noriko</creatorcontrib><creatorcontrib>Wempe, Michael F.</creatorcontrib><creatorcontrib>Takahashi, Hiroyuki</creatorcontrib><creatorcontrib>Inomata, Tomoo</creatorcontrib><creatorcontrib>Ikegami, Masahiro</creatorcontrib><creatorcontrib>Endou, Hitoshi</creatorcontrib><creatorcontrib>Asari, Masao</creatorcontrib><title>Effects of in Utero Exposure to Di(n-butyl) Phthalate for Estrogen Receptors α, β, and Androgen Receptor of Leydig Cell on Rats</title><title>Toxicologic pathology</title><addtitle>Toxicol Pathol</addtitle><description>Estrogens and androgens affect male and female reproductive systems. Recently, we reported that prenatal di(n-butyl) phthalate (DBP) exposure induced atypical Leydig cells (LCs) hyperplasia during adulthood. The present study investigated the expression of estrogen receptor α (ERα), estrogen receptor β (ERβ), and androgen receptor (AR) in LCs of 5-, 7-, 9-, 14-, and 17-week-old Sprague-Dawley (srl) rats whose dams had been administered DBP intragastrically at 100 mg/kg/day or the vehicle (corn oil) from days 12 to 21 postconception. Immunohistochemical, Western blotting, and reverse transcription polymerase chain reaction analyses revealed that the expressions of ERα, ERβ, and AR proteins and mRNAs in the DBP group were similar to those of the vehicle group at 5 and 7 weeks, but significantly higher ERα and lower ERβ and AR levels were observed in the DBP group at 9 to 17 weeks. The rats prenatally exposed to DBP had seminiferous tubule degeneration and atypical hyperplasia of LCs during adulthood, which was associated with an increase in expression of ERα and a decrease of ERβ and AR in the testis.</description><subject>Animals</subject><subject>Dibutyl Phthalate - toxicity</subject><subject>Estrogen Receptor alpha - genetics</subject><subject>Estrogen Receptor alpha - metabolism</subject><subject>Estrogen Receptor beta - genetics</subject><subject>Estrogen Receptor beta - metabolism</subject><subject>Female</subject><subject>Leydig Cells - drug effects</subject><subject>Leydig Cells - metabolism</subject><subject>Male</subject><subject>Pregnancy</subject><subject>Prenatal Exposure Delayed Effects - genetics</subject><subject>Prenatal Exposure Delayed Effects - metabolism</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Receptors, Androgen - genetics</subject><subject>Receptors, Androgen - metabolism</subject><issn>0192-6233</issn><issn>1533-1601</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kEtLw0AQxxdRtD7unmSPFRrdySab9FhqfUBBET2HfUxqJM3W3Q3Yox9JP0g_kylVQcHTHP6PmfkRcgzsDCDLzhkMYxFzDjxlcZ4Nt0gPUs4jEAy2SW8tR2t9j-x7_8wY5JCwXbIXJ0xkIkt65G1SlqiDp7akVUMfAzpLJ68L61uHNFh6UfWbSLVhWZ_Su6fwJGsZkJbW0YkPzs6wofeocRGs83T1PqCrjwGVjaGjxvyW1yumuDTVjI6xrqntJBn8IdkpZe3x6GsekMfLycP4OpreXt2MR9NI84yFCFMYqgRiKZVIc1AJywwDrriODUtSBVKByEWstM5NwoVBkw7BYKJNnIlS8wPS3_QunH1p0YdiXnndHSIbtK0vujTvMPI07axsY9XOeu-wLBaumku3LIAVa_DFX_Bd5OSrvVVzND-Bb9KdIdoYvJxh8Wxb13Tf_l_4CY30i1w</recordid><startdate>20140701</startdate><enddate>20140701</enddate><creator>Wakui, Shin</creator><creator>Shirai, Masaru</creator><creator>Motohashi, Masaya</creator><creator>Mutou, Tomoko</creator><creator>Oyama, Noriko</creator><creator>Wempe, Michael F.</creator><creator>Takahashi, Hiroyuki</creator><creator>Inomata, Tomoo</creator><creator>Ikegami, Masahiro</creator><creator>Endou, Hitoshi</creator><creator>Asari, Masao</creator><general>SAGE Publications</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U7</scope><scope>C1K</scope></search><sort><creationdate>20140701</creationdate><title>Effects of in Utero Exposure to Di(n-butyl) Phthalate for Estrogen Receptors α, β, and Androgen Receptor of Leydig Cell on Rats</title><author>Wakui, Shin ; Shirai, Masaru ; Motohashi, Masaya ; Mutou, Tomoko ; Oyama, Noriko ; Wempe, Michael F. ; Takahashi, Hiroyuki ; Inomata, Tomoo ; Ikegami, Masahiro ; Endou, Hitoshi ; Asari, Masao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c370t-e519b412aab6581b407d013b3c2d045b1ab16862bcc8d436ded591de4cd276fc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Animals</topic><topic>Dibutyl Phthalate - toxicity</topic><topic>Estrogen Receptor alpha - genetics</topic><topic>Estrogen Receptor alpha - metabolism</topic><topic>Estrogen Receptor beta - genetics</topic><topic>Estrogen Receptor beta - metabolism</topic><topic>Female</topic><topic>Leydig Cells - drug effects</topic><topic>Leydig Cells - metabolism</topic><topic>Male</topic><topic>Pregnancy</topic><topic>Prenatal Exposure Delayed Effects - genetics</topic><topic>Prenatal Exposure Delayed Effects - metabolism</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Receptors, Androgen - genetics</topic><topic>Receptors, Androgen - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wakui, Shin</creatorcontrib><creatorcontrib>Shirai, Masaru</creatorcontrib><creatorcontrib>Motohashi, Masaya</creatorcontrib><creatorcontrib>Mutou, Tomoko</creatorcontrib><creatorcontrib>Oyama, Noriko</creatorcontrib><creatorcontrib>Wempe, Michael F.</creatorcontrib><creatorcontrib>Takahashi, Hiroyuki</creatorcontrib><creatorcontrib>Inomata, Tomoo</creatorcontrib><creatorcontrib>Ikegami, Masahiro</creatorcontrib><creatorcontrib>Endou, Hitoshi</creatorcontrib><creatorcontrib>Asari, Masao</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Toxicologic pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wakui, Shin</au><au>Shirai, Masaru</au><au>Motohashi, Masaya</au><au>Mutou, Tomoko</au><au>Oyama, Noriko</au><au>Wempe, Michael F.</au><au>Takahashi, Hiroyuki</au><au>Inomata, Tomoo</au><au>Ikegami, Masahiro</au><au>Endou, Hitoshi</au><au>Asari, Masao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of in Utero Exposure to Di(n-butyl) Phthalate for Estrogen Receptors α, β, and Androgen Receptor of Leydig Cell on Rats</atitle><jtitle>Toxicologic pathology</jtitle><addtitle>Toxicol Pathol</addtitle><date>2014-07-01</date><risdate>2014</risdate><volume>42</volume><issue>5</issue><spage>877</spage><epage>887</epage><pages>877-887</pages><issn>0192-6233</issn><eissn>1533-1601</eissn><abstract>Estrogens and androgens affect male and female reproductive systems. Recently, we reported that prenatal di(n-butyl) phthalate (DBP) exposure induced atypical Leydig cells (LCs) hyperplasia during adulthood. The present study investigated the expression of estrogen receptor α (ERα), estrogen receptor β (ERβ), and androgen receptor (AR) in LCs of 5-, 7-, 9-, 14-, and 17-week-old Sprague-Dawley (srl) rats whose dams had been administered DBP intragastrically at 100 mg/kg/day or the vehicle (corn oil) from days 12 to 21 postconception. Immunohistochemical, Western blotting, and reverse transcription polymerase chain reaction analyses revealed that the expressions of ERα, ERβ, and AR proteins and mRNAs in the DBP group were similar to those of the vehicle group at 5 and 7 weeks, but significantly higher ERα and lower ERβ and AR levels were observed in the DBP group at 9 to 17 weeks. 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| ispartof | Toxicologic pathology, 2014-07, Vol.42 (5), p.877-887 |
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| source | SAGE Complete A-Z List; MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Dibutyl Phthalate - toxicity Estrogen Receptor alpha - genetics Estrogen Receptor alpha - metabolism Estrogen Receptor beta - genetics Estrogen Receptor beta - metabolism Female Leydig Cells - drug effects Leydig Cells - metabolism Male Pregnancy Prenatal Exposure Delayed Effects - genetics Prenatal Exposure Delayed Effects - metabolism Rats Rats, Sprague-Dawley Receptors, Androgen - genetics Receptors, Androgen - metabolism |
| title | Effects of in Utero Exposure to Di(n-butyl) Phthalate for Estrogen Receptors α, β, and Androgen Receptor of Leydig Cell on Rats |
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