Low expression of secreted frizzled-related protein 4 in aggressive pituitary adenoma
Objective The secreted frizzled-related proteins (sFRPs) are reported to be antagonists of a number of tumors. This study was designed to investigate the relationship of sFRP4 with aggressiveness of pituitary adenomas. Material and method Specimens were classified into three groups: normal control (...
Gespeichert in:
| Veröffentlicht in: | Pituitary 2015-06, Vol.18 (3), p.335-342 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 342 |
|---|---|
| container_issue | 3 |
| container_start_page | 335 |
| container_title | Pituitary |
| container_volume | 18 |
| creator | Wu, Youtu Bai, Jiwei Li, Zhenye Wang, Fei Cao, Lei Liu, Chunhui Yu, Shengyuan Yu, Guoqiang Zhang, Yazhuo |
| description | Objective
The secreted frizzled-related proteins (sFRPs) are reported to be antagonists of a number of tumors. This study was designed to investigate the relationship of sFRP4 with aggressiveness of pituitary adenomas.
Material and method
Specimens were classified into three groups: normal control (n = 10), non-aggressive group (n = 42) and aggressive group (n = 26) according to preoperative magnetic resonance imaging (MRI)/computed tomography. sFRP4 were investigated by PCR, Western blotting, and immunohistochemistry (IHC). The methylation status of the sFRP4 promoter region was observed by MassArray. Cell culture and 5-aza-2-deoxycytidine treatment was performed to observe the relationship of downregulation of sFRP4 with methylation of the
sFRP4
gene.
Results
PCR and Western blot results showed that sFRP4 expression was downregulated in aggressive pituitary adenomas, which was confirmed by IHC. Methylation of the sFRP4 promoter was increased in aggressive pituitary adenomas. And methylation of the sFRP4 promoter lead to downregulation of sFRP4 expression.
Conclusions
sFRP4 expression is inversely related to the aggressiveness of pituitary adenomas, and act as a tumor suppressor. |
| doi_str_mv | 10.1007/s11102-014-0579-4 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1683349412</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1683349412</sourcerecordid><originalsourceid>FETCH-LOGICAL-c475t-901a5f76bf2805e54b9b4943c5168235dd64aa863a45954a0e024309a814cb9f3</originalsourceid><addsrcrecordid>eNqNkctKxDAUhoMo3h_AjRTcuInmNCdpsxTxBgNuFNyFtD0dKp12TFov8_RmHBURBDdNSb7_y-Vn7ADECQiRnQYAECkXgFyozHBcY9ugMskzFHI9_stcc4nwsMV2QngUIqYkbrKtFA1kUsM2u5_0Lwm9zj2F0PRd0tdJoNLTQFVS-2axaKninlq3nJj7fqCmSzCJHzedfoSeKZk3w9gMzr8lrqKun7k9tlG7NtD-57jL7i8v7s6v-eT26ub8bMJLzNTAjQCn6kwXdZoLRQoLU6BBWSrQeSpVVWl0LtfSoTIKnSCRohTG5YBlYWq5y45X3niyp5HCYGdNKKltXUf9GGzUSBmNkP4HFZDnaFREj36hj_3ou3iRSGVG6lzrpRBWVOn7EDzVdu6bWXwEC8Iu67Gremysxy7rsRgzh5_msZhR9Z346iMC6QoIcambkv-x9Z_Wd_NAmNQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1679368662</pqid></control><display><type>article</type><title>Low expression of secreted frizzled-related protein 4 in aggressive pituitary adenoma</title><source>MEDLINE</source><source>Springer Nature - Complete Springer Journals</source><creator>Wu, Youtu ; Bai, Jiwei ; Li, Zhenye ; Wang, Fei ; Cao, Lei ; Liu, Chunhui ; Yu, Shengyuan ; Yu, Guoqiang ; Zhang, Yazhuo</creator><creatorcontrib>Wu, Youtu ; Bai, Jiwei ; Li, Zhenye ; Wang, Fei ; Cao, Lei ; Liu, Chunhui ; Yu, Shengyuan ; Yu, Guoqiang ; Zhang, Yazhuo</creatorcontrib><description>Objective
The secreted frizzled-related proteins (sFRPs) are reported to be antagonists of a number of tumors. This study was designed to investigate the relationship of sFRP4 with aggressiveness of pituitary adenomas.
Material and method
Specimens were classified into three groups: normal control (n = 10), non-aggressive group (n = 42) and aggressive group (n = 26) according to preoperative magnetic resonance imaging (MRI)/computed tomography. sFRP4 were investigated by PCR, Western blotting, and immunohistochemistry (IHC). The methylation status of the sFRP4 promoter region was observed by MassArray. Cell culture and 5-aza-2-deoxycytidine treatment was performed to observe the relationship of downregulation of sFRP4 with methylation of the
sFRP4
gene.
Results
PCR and Western blot results showed that sFRP4 expression was downregulated in aggressive pituitary adenomas, which was confirmed by IHC. Methylation of the sFRP4 promoter was increased in aggressive pituitary adenomas. And methylation of the sFRP4 promoter lead to downregulation of sFRP4 expression.
Conclusions
sFRP4 expression is inversely related to the aggressiveness of pituitary adenomas, and act as a tumor suppressor.</description><identifier>ISSN: 1386-341X</identifier><identifier>EISSN: 1573-7403</identifier><identifier>DOI: 10.1007/s11102-014-0579-4</identifier><identifier>PMID: 24917361</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Adenoma - genetics ; Adenoma - metabolism ; Adenoma - pathology ; Adolescent ; Adult ; Aged ; Biomarkers, Tumor - genetics ; Biomarkers, Tumor - metabolism ; Blotting, Western ; Case-Control Studies ; Cell Line ; CpG Islands ; DNA Methylation ; Down-Regulation ; Endocrinology ; Female ; Gene Expression Regulation, Neoplastic ; Human Physiology ; Humans ; Immunohistochemistry ; Magnetic Resonance Imaging ; Male ; Medicine ; Medicine & Public Health ; Middle Aged ; Pituitary Neoplasms - genetics ; Pituitary Neoplasms - metabolism ; Pituitary Neoplasms - pathology ; Polymerase Chain Reaction ; Promoter Regions, Genetic ; Proto-Oncogene Proteins - genetics ; Proto-Oncogene Proteins - metabolism ; RNA, Messenger - genetics ; Tomography, X-Ray Computed ; Transfection ; Tumor Suppressor Proteins - genetics ; Tumor Suppressor Proteins - metabolism ; Young Adult</subject><ispartof>Pituitary, 2015-06, Vol.18 (3), p.335-342</ispartof><rights>Springer Science+Business Media New York 2014</rights><rights>Springer Science+Business Media New York 2015</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c475t-901a5f76bf2805e54b9b4943c5168235dd64aa863a45954a0e024309a814cb9f3</citedby><cites>FETCH-LOGICAL-c475t-901a5f76bf2805e54b9b4943c5168235dd64aa863a45954a0e024309a814cb9f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11102-014-0579-4$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11102-014-0579-4$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,777,781,27905,27906,41469,42538,51300</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24917361$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wu, Youtu</creatorcontrib><creatorcontrib>Bai, Jiwei</creatorcontrib><creatorcontrib>Li, Zhenye</creatorcontrib><creatorcontrib>Wang, Fei</creatorcontrib><creatorcontrib>Cao, Lei</creatorcontrib><creatorcontrib>Liu, Chunhui</creatorcontrib><creatorcontrib>Yu, Shengyuan</creatorcontrib><creatorcontrib>Yu, Guoqiang</creatorcontrib><creatorcontrib>Zhang, Yazhuo</creatorcontrib><title>Low expression of secreted frizzled-related protein 4 in aggressive pituitary adenoma</title><title>Pituitary</title><addtitle>Pituitary</addtitle><addtitle>Pituitary</addtitle><description>Objective
The secreted frizzled-related proteins (sFRPs) are reported to be antagonists of a number of tumors. This study was designed to investigate the relationship of sFRP4 with aggressiveness of pituitary adenomas.
Material and method
Specimens were classified into three groups: normal control (n = 10), non-aggressive group (n = 42) and aggressive group (n = 26) according to preoperative magnetic resonance imaging (MRI)/computed tomography. sFRP4 were investigated by PCR, Western blotting, and immunohistochemistry (IHC). The methylation status of the sFRP4 promoter region was observed by MassArray. Cell culture and 5-aza-2-deoxycytidine treatment was performed to observe the relationship of downregulation of sFRP4 with methylation of the
sFRP4
gene.
Results
PCR and Western blot results showed that sFRP4 expression was downregulated in aggressive pituitary adenomas, which was confirmed by IHC. Methylation of the sFRP4 promoter was increased in aggressive pituitary adenomas. And methylation of the sFRP4 promoter lead to downregulation of sFRP4 expression.
Conclusions
sFRP4 expression is inversely related to the aggressiveness of pituitary adenomas, and act as a tumor suppressor.</description><subject>Adenoma - genetics</subject><subject>Adenoma - metabolism</subject><subject>Adenoma - pathology</subject><subject>Adolescent</subject><subject>Adult</subject><subject>Aged</subject><subject>Biomarkers, Tumor - genetics</subject><subject>Biomarkers, Tumor - metabolism</subject><subject>Blotting, Western</subject><subject>Case-Control Studies</subject><subject>Cell Line</subject><subject>CpG Islands</subject><subject>DNA Methylation</subject><subject>Down-Regulation</subject><subject>Endocrinology</subject><subject>Female</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Human Physiology</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Magnetic Resonance Imaging</subject><subject>Male</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Middle Aged</subject><subject>Pituitary Neoplasms - genetics</subject><subject>Pituitary Neoplasms - metabolism</subject><subject>Pituitary Neoplasms - pathology</subject><subject>Polymerase Chain Reaction</subject><subject>Promoter Regions, Genetic</subject><subject>Proto-Oncogene Proteins - genetics</subject><subject>Proto-Oncogene Proteins - metabolism</subject><subject>RNA, Messenger - genetics</subject><subject>Tomography, X-Ray Computed</subject><subject>Transfection</subject><subject>Tumor Suppressor Proteins - genetics</subject><subject>Tumor Suppressor Proteins - metabolism</subject><subject>Young Adult</subject><issn>1386-341X</issn><issn>1573-7403</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNqNkctKxDAUhoMo3h_AjRTcuInmNCdpsxTxBgNuFNyFtD0dKp12TFov8_RmHBURBDdNSb7_y-Vn7ADECQiRnQYAECkXgFyozHBcY9ugMskzFHI9_stcc4nwsMV2QngUIqYkbrKtFA1kUsM2u5_0Lwm9zj2F0PRd0tdJoNLTQFVS-2axaKninlq3nJj7fqCmSzCJHzedfoSeKZk3w9gMzr8lrqKun7k9tlG7NtD-57jL7i8v7s6v-eT26ub8bMJLzNTAjQCn6kwXdZoLRQoLU6BBWSrQeSpVVWl0LtfSoTIKnSCRohTG5YBlYWq5y45X3niyp5HCYGdNKKltXUf9GGzUSBmNkP4HFZDnaFREj36hj_3ou3iRSGVG6lzrpRBWVOn7EDzVdu6bWXwEC8Iu67Gremysxy7rsRgzh5_msZhR9Z346iMC6QoIcambkv-x9Z_Wd_NAmNQ</recordid><startdate>20150601</startdate><enddate>20150601</enddate><creator>Wu, Youtu</creator><creator>Bai, Jiwei</creator><creator>Li, Zhenye</creator><creator>Wang, Fei</creator><creator>Cao, Lei</creator><creator>Liu, Chunhui</creator><creator>Yu, Shengyuan</creator><creator>Yu, Guoqiang</creator><creator>Zhang, Yazhuo</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20150601</creationdate><title>Low expression of secreted frizzled-related protein 4 in aggressive pituitary adenoma</title><author>Wu, Youtu ; Bai, Jiwei ; Li, Zhenye ; Wang, Fei ; Cao, Lei ; Liu, Chunhui ; Yu, Shengyuan ; Yu, Guoqiang ; Zhang, Yazhuo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c475t-901a5f76bf2805e54b9b4943c5168235dd64aa863a45954a0e024309a814cb9f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Adenoma - genetics</topic><topic>Adenoma - metabolism</topic><topic>Adenoma - pathology</topic><topic>Adolescent</topic><topic>Adult</topic><topic>Aged</topic><topic>Biomarkers, Tumor - genetics</topic><topic>Biomarkers, Tumor - metabolism</topic><topic>Blotting, Western</topic><topic>Case-Control Studies</topic><topic>Cell Line</topic><topic>CpG Islands</topic><topic>DNA Methylation</topic><topic>Down-Regulation</topic><topic>Endocrinology</topic><topic>Female</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Human Physiology</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Magnetic Resonance Imaging</topic><topic>Male</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Middle Aged</topic><topic>Pituitary Neoplasms - genetics</topic><topic>Pituitary Neoplasms - metabolism</topic><topic>Pituitary Neoplasms - pathology</topic><topic>Polymerase Chain Reaction</topic><topic>Promoter Regions, Genetic</topic><topic>Proto-Oncogene Proteins - genetics</topic><topic>Proto-Oncogene Proteins - metabolism</topic><topic>RNA, Messenger - genetics</topic><topic>Tomography, X-Ray Computed</topic><topic>Transfection</topic><topic>Tumor Suppressor Proteins - genetics</topic><topic>Tumor Suppressor Proteins - metabolism</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wu, Youtu</creatorcontrib><creatorcontrib>Bai, Jiwei</creatorcontrib><creatorcontrib>Li, Zhenye</creatorcontrib><creatorcontrib>Wang, Fei</creatorcontrib><creatorcontrib>Cao, Lei</creatorcontrib><creatorcontrib>Liu, Chunhui</creatorcontrib><creatorcontrib>Yu, Shengyuan</creatorcontrib><creatorcontrib>Yu, Guoqiang</creatorcontrib><creatorcontrib>Zhang, Yazhuo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Pituitary</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wu, Youtu</au><au>Bai, Jiwei</au><au>Li, Zhenye</au><au>Wang, Fei</au><au>Cao, Lei</au><au>Liu, Chunhui</au><au>Yu, Shengyuan</au><au>Yu, Guoqiang</au><au>Zhang, Yazhuo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Low expression of secreted frizzled-related protein 4 in aggressive pituitary adenoma</atitle><jtitle>Pituitary</jtitle><stitle>Pituitary</stitle><addtitle>Pituitary</addtitle><date>2015-06-01</date><risdate>2015</risdate><volume>18</volume><issue>3</issue><spage>335</spage><epage>342</epage><pages>335-342</pages><issn>1386-341X</issn><eissn>1573-7403</eissn><abstract>Objective
The secreted frizzled-related proteins (sFRPs) are reported to be antagonists of a number of tumors. This study was designed to investigate the relationship of sFRP4 with aggressiveness of pituitary adenomas.
Material and method
Specimens were classified into three groups: normal control (n = 10), non-aggressive group (n = 42) and aggressive group (n = 26) according to preoperative magnetic resonance imaging (MRI)/computed tomography. sFRP4 were investigated by PCR, Western blotting, and immunohistochemistry (IHC). The methylation status of the sFRP4 promoter region was observed by MassArray. Cell culture and 5-aza-2-deoxycytidine treatment was performed to observe the relationship of downregulation of sFRP4 with methylation of the
sFRP4
gene.
Results
PCR and Western blot results showed that sFRP4 expression was downregulated in aggressive pituitary adenomas, which was confirmed by IHC. Methylation of the sFRP4 promoter was increased in aggressive pituitary adenomas. And methylation of the sFRP4 promoter lead to downregulation of sFRP4 expression.
Conclusions
sFRP4 expression is inversely related to the aggressiveness of pituitary adenomas, and act as a tumor suppressor.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>24917361</pmid><doi>10.1007/s11102-014-0579-4</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1386-341X |
| ispartof | Pituitary, 2015-06, Vol.18 (3), p.335-342 |
| issn | 1386-341X 1573-7403 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_1683349412 |
| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Adenoma - genetics Adenoma - metabolism Adenoma - pathology Adolescent Adult Aged Biomarkers, Tumor - genetics Biomarkers, Tumor - metabolism Blotting, Western Case-Control Studies Cell Line CpG Islands DNA Methylation Down-Regulation Endocrinology Female Gene Expression Regulation, Neoplastic Human Physiology Humans Immunohistochemistry Magnetic Resonance Imaging Male Medicine Medicine & Public Health Middle Aged Pituitary Neoplasms - genetics Pituitary Neoplasms - metabolism Pituitary Neoplasms - pathology Polymerase Chain Reaction Promoter Regions, Genetic Proto-Oncogene Proteins - genetics Proto-Oncogene Proteins - metabolism RNA, Messenger - genetics Tomography, X-Ray Computed Transfection Tumor Suppressor Proteins - genetics Tumor Suppressor Proteins - metabolism Young Adult |
| title | Low expression of secreted frizzled-related protein 4 in aggressive pituitary adenoma |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-20T18%3A48%3A36IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Low%20expression%20of%20secreted%20frizzled-related%20protein%204%20in%20aggressive%20pituitary%20adenoma&rft.jtitle=Pituitary&rft.au=Wu,%20Youtu&rft.date=2015-06-01&rft.volume=18&rft.issue=3&rft.spage=335&rft.epage=342&rft.pages=335-342&rft.issn=1386-341X&rft.eissn=1573-7403&rft_id=info:doi/10.1007/s11102-014-0579-4&rft_dat=%3Cproquest_cross%3E1683349412%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1679368662&rft_id=info:pmid/24917361&rfr_iscdi=true |