Optimization of sporicidal activity and environmental Bacillus endospores decontamination by biogenic silver nanoparticle
The intent of this study is to decontaminate Bacillus endospores and to determine the D-values using silver nanoparticles (AgNPs) synthesized from Streptomyces sp. cell filtrate. AgNPs synthesis was performed extracellularly followed by characterization using spectrophotometer, High Resolution Trans...
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| Veröffentlicht in: | Future microbiology 2015-05, Vol.10 (5), p.725-741 |
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| creator | Gopinath, Ponnusamy M Dhanasekaran, Dharumadhurai Ranjani, Anandan Thajuddin, Nooruddin Akbarsha, Mohamad A Velmurugan, Meganathan Panneerselvam, Annamalai |
| description | The intent of this study is to decontaminate Bacillus endospores and to determine the D-values using silver nanoparticles (AgNPs) synthesized from Streptomyces sp. cell filtrate.
AgNPs synthesis was performed extracellularly followed by characterization using spectrophotometer, High Resolution Transmission Electron Microscope and x-ray diffraction pattern analysis. Subsequently, the optimized conditions for the decontamination and D-value estimation of Bacillus endospores were determined using the response surface methodology. The environmental spore decontamination study was performed in mice model.
AgNPs were visibly and spectroscopically identified which were spherical with the size range of less than 20 nm. The synthesized AgNPs destroyed 1log10 CFU Bacillus endospores at around 20 min. The adherence of AgNPs to the surface of spore coat, pit formation and its complete structural loss was detected under field emission scanning electron microscopy. All the mice exposed to AgNP-treated spores showed no sign of pathological lesions.
The results of our study strongly suggest that the application of AgNPs as a sporicidal agent could be a new approach in consistently eliminating the hazardous Bacillus spores. |
| doi_str_mv | 10.2217/fmb.14.150 |
| format | Article |
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AgNPs synthesis was performed extracellularly followed by characterization using spectrophotometer, High Resolution Transmission Electron Microscope and x-ray diffraction pattern analysis. Subsequently, the optimized conditions for the decontamination and D-value estimation of Bacillus endospores were determined using the response surface methodology. The environmental spore decontamination study was performed in mice model.
AgNPs were visibly and spectroscopically identified which were spherical with the size range of less than 20 nm. The synthesized AgNPs destroyed 1log10 CFU Bacillus endospores at around 20 min. The adherence of AgNPs to the surface of spore coat, pit formation and its complete structural loss was detected under field emission scanning electron microscopy. All the mice exposed to AgNP-treated spores showed no sign of pathological lesions.
The results of our study strongly suggest that the application of AgNPs as a sporicidal agent could be a new approach in consistently eliminating the hazardous Bacillus spores.</description><identifier>ISSN: 1746-0913</identifier><identifier>EISSN: 1746-0921</identifier><identifier>DOI: 10.2217/fmb.14.150</identifier><identifier>PMID: 26000648</identifier><language>eng</language><publisher>England: Future Medicine Ltd</publisher><subject>Animals ; Bacillus ; Bacillus - drug effects ; Bacterial spores ; Biological & chemical terrorism ; Decontamination ; Disease Models, Animal ; Disinfectants - isolation & purification ; Disinfectants - pharmacology ; Disinfection - methods ; Drug resistance ; Environmental Microbiology ; Fourier transforms ; Gram-Positive Bacterial Infections - pathology ; Health aspects ; Methods ; Mice ; Microbial Viability - drug effects ; Microscopy, Electron, Scanning ; Microscopy, Electron, Transmission ; Nanoparticles ; Nanotechnology ; Optimization ; Scholarships & fellowships ; Silver ; Silver - isolation & purification ; Silver - pharmacology ; Spectrophotometry ; Spores, Bacterial - drug effects ; Streptomyces - metabolism ; Time Factors ; X-Ray Diffraction</subject><ispartof>Future microbiology, 2015-05, Vol.10 (5), p.725-741</ispartof><rights>COPYRIGHT 2015 Future Medicine Ltd.</rights><rights>2015 Future Medicine Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c382t-19d26ed5b806dce4040c5ebb8bbc4580a911169d92cd448994279af65ed3435d3</citedby><cites>FETCH-LOGICAL-c382t-19d26ed5b806dce4040c5ebb8bbc4580a911169d92cd448994279af65ed3435d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26000648$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gopinath, Ponnusamy M</creatorcontrib><creatorcontrib>Dhanasekaran, Dharumadhurai</creatorcontrib><creatorcontrib>Ranjani, Anandan</creatorcontrib><creatorcontrib>Thajuddin, Nooruddin</creatorcontrib><creatorcontrib>Akbarsha, Mohamad A</creatorcontrib><creatorcontrib>Velmurugan, Meganathan</creatorcontrib><creatorcontrib>Panneerselvam, Annamalai</creatorcontrib><title>Optimization of sporicidal activity and environmental Bacillus endospores decontamination by biogenic silver nanoparticle</title><title>Future microbiology</title><addtitle>Future Microbiol</addtitle><description>The intent of this study is to decontaminate Bacillus endospores and to determine the D-values using silver nanoparticles (AgNPs) synthesized from Streptomyces sp. cell filtrate.
AgNPs synthesis was performed extracellularly followed by characterization using spectrophotometer, High Resolution Transmission Electron Microscope and x-ray diffraction pattern analysis. Subsequently, the optimized conditions for the decontamination and D-value estimation of Bacillus endospores were determined using the response surface methodology. The environmental spore decontamination study was performed in mice model.
AgNPs were visibly and spectroscopically identified which were spherical with the size range of less than 20 nm. The synthesized AgNPs destroyed 1log10 CFU Bacillus endospores at around 20 min. The adherence of AgNPs to the surface of spore coat, pit formation and its complete structural loss was detected under field emission scanning electron microscopy. All the mice exposed to AgNP-treated spores showed no sign of pathological lesions.
The results of our study strongly suggest that the application of AgNPs as a sporicidal agent could be a new approach in consistently eliminating the hazardous Bacillus spores.</description><subject>Animals</subject><subject>Bacillus</subject><subject>Bacillus - drug effects</subject><subject>Bacterial spores</subject><subject>Biological & chemical terrorism</subject><subject>Decontamination</subject><subject>Disease Models, Animal</subject><subject>Disinfectants - isolation & purification</subject><subject>Disinfectants - pharmacology</subject><subject>Disinfection - methods</subject><subject>Drug resistance</subject><subject>Environmental Microbiology</subject><subject>Fourier transforms</subject><subject>Gram-Positive Bacterial Infections - pathology</subject><subject>Health aspects</subject><subject>Methods</subject><subject>Mice</subject><subject>Microbial Viability - drug effects</subject><subject>Microscopy, Electron, Scanning</subject><subject>Microscopy, Electron, Transmission</subject><subject>Nanoparticles</subject><subject>Nanotechnology</subject><subject>Optimization</subject><subject>Scholarships & fellowships</subject><subject>Silver</subject><subject>Silver - isolation & purification</subject><subject>Silver - pharmacology</subject><subject>Spectrophotometry</subject><subject>Spores, Bacterial - drug effects</subject><subject>Streptomyces - metabolism</subject><subject>Time Factors</subject><subject>X-Ray Diffraction</subject><issn>1746-0913</issn><issn>1746-0921</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNptkUtr3DAUhU1paNK0m_6AYuimFGaqK0uyvUxCH4FANula6HEdbrAlV7IHpr--GiZNaQlaSNz7ncNBp6reAdtyDu3nYbJbEFuQ7EV1Bq1QG9ZzePn0hua0ep3zA2Oygx5eVadcMcaU6M6q_e280ES_zEIx1HGo8xwTOfJmrI1baEfLvjbB1xh2lGKYMCxldWkcjeOay9jHgwRz7dHFspwoHM3svrYU7zGQqzONO0x1MCHOJi3kRnxTnQxmzPj28T6vfnz9cnf1fXNz--366uJm45qOLxvoPVfope2Y8g4FE8xJtLaz1gnZMdMDgOp9z50Xout7wdveDEqib0QjfXNefTz6zin-XDEveqLscBxNwLhmDaprWKsEQEE__Ic-xDWFku5AcdW0Soq_1L0ZUVMY4pKMO5jqCwFClkCsLdT2GaocjxOVj8KByvwfwaejwKWYc8JBz4kmk_YamD70rEvPGoQuPRf4_WPS1U7on9A_xTa_ARBCo8c</recordid><startdate>20150501</startdate><enddate>20150501</enddate><creator>Gopinath, Ponnusamy M</creator><creator>Dhanasekaran, Dharumadhurai</creator><creator>Ranjani, Anandan</creator><creator>Thajuddin, Nooruddin</creator><creator>Akbarsha, Mohamad A</creator><creator>Velmurugan, Meganathan</creator><creator>Panneerselvam, Annamalai</creator><general>Future Medicine Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>EHMNL</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20150501</creationdate><title>Optimization of sporicidal activity and environmental Bacillus endospores decontamination by biogenic silver nanoparticle</title><author>Gopinath, Ponnusamy M ; Dhanasekaran, Dharumadhurai ; Ranjani, Anandan ; Thajuddin, Nooruddin ; Akbarsha, Mohamad A ; Velmurugan, Meganathan ; Panneerselvam, Annamalai</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c382t-19d26ed5b806dce4040c5ebb8bbc4580a911169d92cd448994279af65ed3435d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Animals</topic><topic>Bacillus</topic><topic>Bacillus - drug effects</topic><topic>Bacterial spores</topic><topic>Biological & chemical terrorism</topic><topic>Decontamination</topic><topic>Disease Models, Animal</topic><topic>Disinfectants - isolation & purification</topic><topic>Disinfectants - pharmacology</topic><topic>Disinfection - methods</topic><topic>Drug resistance</topic><topic>Environmental Microbiology</topic><topic>Fourier transforms</topic><topic>Gram-Positive Bacterial Infections - pathology</topic><topic>Health aspects</topic><topic>Methods</topic><topic>Mice</topic><topic>Microbial Viability - drug effects</topic><topic>Microscopy, Electron, Scanning</topic><topic>Microscopy, Electron, Transmission</topic><topic>Nanoparticles</topic><topic>Nanotechnology</topic><topic>Optimization</topic><topic>Scholarships & fellowships</topic><topic>Silver</topic><topic>Silver - isolation & purification</topic><topic>Silver - pharmacology</topic><topic>Spectrophotometry</topic><topic>Spores, Bacterial - drug effects</topic><topic>Streptomyces - metabolism</topic><topic>Time Factors</topic><topic>X-Ray Diffraction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gopinath, Ponnusamy M</creatorcontrib><creatorcontrib>Dhanasekaran, Dharumadhurai</creatorcontrib><creatorcontrib>Ranjani, Anandan</creatorcontrib><creatorcontrib>Thajuddin, Nooruddin</creatorcontrib><creatorcontrib>Akbarsha, Mohamad A</creatorcontrib><creatorcontrib>Velmurugan, Meganathan</creatorcontrib><creatorcontrib>Panneerselvam, Annamalai</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>UK & Ireland Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Future microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gopinath, Ponnusamy M</au><au>Dhanasekaran, Dharumadhurai</au><au>Ranjani, Anandan</au><au>Thajuddin, Nooruddin</au><au>Akbarsha, Mohamad A</au><au>Velmurugan, Meganathan</au><au>Panneerselvam, Annamalai</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Optimization of sporicidal activity and environmental Bacillus endospores decontamination by biogenic silver nanoparticle</atitle><jtitle>Future microbiology</jtitle><addtitle>Future Microbiol</addtitle><date>2015-05-01</date><risdate>2015</risdate><volume>10</volume><issue>5</issue><spage>725</spage><epage>741</epage><pages>725-741</pages><issn>1746-0913</issn><eissn>1746-0921</eissn><abstract>The intent of this study is to decontaminate Bacillus endospores and to determine the D-values using silver nanoparticles (AgNPs) synthesized from Streptomyces sp. cell filtrate.
AgNPs synthesis was performed extracellularly followed by characterization using spectrophotometer, High Resolution Transmission Electron Microscope and x-ray diffraction pattern analysis. Subsequently, the optimized conditions for the decontamination and D-value estimation of Bacillus endospores were determined using the response surface methodology. The environmental spore decontamination study was performed in mice model.
AgNPs were visibly and spectroscopically identified which were spherical with the size range of less than 20 nm. The synthesized AgNPs destroyed 1log10 CFU Bacillus endospores at around 20 min. The adherence of AgNPs to the surface of spore coat, pit formation and its complete structural loss was detected under field emission scanning electron microscopy. All the mice exposed to AgNP-treated spores showed no sign of pathological lesions.
The results of our study strongly suggest that the application of AgNPs as a sporicidal agent could be a new approach in consistently eliminating the hazardous Bacillus spores.</abstract><cop>England</cop><pub>Future Medicine Ltd</pub><pmid>26000648</pmid><doi>10.2217/fmb.14.150</doi><tpages>17</tpages></addata></record> |
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| subjects | Animals Bacillus Bacillus - drug effects Bacterial spores Biological & chemical terrorism Decontamination Disease Models, Animal Disinfectants - isolation & purification Disinfectants - pharmacology Disinfection - methods Drug resistance Environmental Microbiology Fourier transforms Gram-Positive Bacterial Infections - pathology Health aspects Methods Mice Microbial Viability - drug effects Microscopy, Electron, Scanning Microscopy, Electron, Transmission Nanoparticles Nanotechnology Optimization Scholarships & fellowships Silver Silver - isolation & purification Silver - pharmacology Spectrophotometry Spores, Bacterial - drug effects Streptomyces - metabolism Time Factors X-Ray Diffraction |
| title | Optimization of sporicidal activity and environmental Bacillus endospores decontamination by biogenic silver nanoparticle |
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