Exogenous tyrosol inhibits planktonic cells and biofilms of Candida species and enhances their susceptibility to antifungals
Tyrosol is a quorum-sensing molecule of Candida albicans able to induce hyphal development in the early and intermediate stages of biofilm growth. In the present study, we evaluated the effect of high concentrations of exogenous tyrosol on planktonic cells and biofilms of C. albicans (n = 10) and C....
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| creator | Cordeiro, Rossana de A. Teixeira, Carlos E.C. Brilhante, Raimunda S.N. Castelo-Branco, Débora S.C.M. Alencar, Lucas P. de Oliveira, Jonathas S. Monteiro, André J. Bandeira, Tereza J.P.G. Sidrim, José J.C. Moreira, José Luciano Bezerra Rocha, Marcos F.G. |
| description | Tyrosol is a quorum-sensing molecule of Candida albicans able to induce hyphal development in the early and intermediate stages of biofilm growth. In the present study, we evaluated the effect of high concentrations of exogenous tyrosol on planktonic cells and biofilms of C. albicans (n = 10) and C. tropicalis (n = 10), and investigated whether tyrosol could be synergic to antifungals that target cellular ergosterol. Antifungal susceptibility and drug interaction against planktonic cells were investigated by the broth microdilution method. Tyrosol was able to inhibit planktonic cells, with MIC values ranging from 2.5 to 5.0 mM for both species. Synergism was observed between tyrosol/amphotericin B (11/20 strains), tyrosol/itraconazole (18/20 strains) and tyrosol/fluconazole (18/20 strains). Exogenous tyrosol alone or combined with antifungals at both 10 × MIC and 50 × MIC were able to reduce biofilm of both Candida species. Mature biofilms were susceptible to tyrosol alone at 50 × MIC or combined with amphotericin at both 10 × MIC and 50 × MIC. On the other hand, tyrosol plus azoles at both 10 × MIC and 50 × MIC enhanced biofilm growth.
Exogenous tyrosol destroys Candida albicans and C. tropicalis biofilms. |
| doi_str_mv | 10.1093/femsyr/fov012 |
| format | Article |
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Exogenous tyrosol destroys Candida albicans and C. tropicalis biofilms.</description><identifier>ISSN: 1567-1356</identifier><identifier>EISSN: 1567-1364</identifier><identifier>DOI: 10.1093/femsyr/fov012</identifier><identifier>PMID: 25795651</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Amphotericin B ; Amphotericin B - metabolism ; Antifungal agents ; Antifungal Agents - metabolism ; Azoles ; Biofilms ; Biofilms - drug effects ; Candida ; Candida - drug effects ; Candida - physiology ; Candida albicans ; Drug interaction ; Drug Synergism ; Ergosterol ; Fluconazole ; Fluconazole - metabolism ; Itraconazole ; Itraconazole - metabolism ; Microbial Sensitivity Tests ; Minimum inhibitory concentration ; Phenylethyl Alcohol - analogs & derivatives ; Phenylethyl Alcohol - metabolism ; Planktonic cells ; Species ; Tyrosol</subject><ispartof>FEMS yeast research, 2015-06, Vol.15 (4), p.fov012-fov012</ispartof><rights>FEMS 2015. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com 2015</rights><rights>FEMS 2015. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.</rights><rights>FEMS 2015. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c354t-eab20aed6c5753349bb3371aa951e6fc53222364f672b8fcc8ce2dcc4c7ffaa83</citedby><cites>FETCH-LOGICAL-c354t-eab20aed6c5753349bb3371aa951e6fc53222364f672b8fcc8ce2dcc4c7ffaa83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1598,27901,27902</link.rule.ids><linktorsrc>$$Uhttps://dx.doi.org/10.1093/femsyr/fov012$$EView_record_in_Oxford_University_Press$$FView_record_in_$$GOxford_University_Press</linktorsrc><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25795651$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cordeiro, Rossana de A.</creatorcontrib><creatorcontrib>Teixeira, Carlos E.C.</creatorcontrib><creatorcontrib>Brilhante, Raimunda S.N.</creatorcontrib><creatorcontrib>Castelo-Branco, Débora S.C.M.</creatorcontrib><creatorcontrib>Alencar, Lucas P.</creatorcontrib><creatorcontrib>de Oliveira, Jonathas S.</creatorcontrib><creatorcontrib>Monteiro, André J.</creatorcontrib><creatorcontrib>Bandeira, Tereza J.P.G.</creatorcontrib><creatorcontrib>Sidrim, José J.C.</creatorcontrib><creatorcontrib>Moreira, José Luciano Bezerra</creatorcontrib><creatorcontrib>Rocha, Marcos F.G.</creatorcontrib><title>Exogenous tyrosol inhibits planktonic cells and biofilms of Candida species and enhances their susceptibility to antifungals</title><title>FEMS yeast research</title><addtitle>FEMS Yeast Res</addtitle><description>Tyrosol is a quorum-sensing molecule of Candida albicans able to induce hyphal development in the early and intermediate stages of biofilm growth. In the present study, we evaluated the effect of high concentrations of exogenous tyrosol on planktonic cells and biofilms of C. albicans (n = 10) and C. tropicalis (n = 10), and investigated whether tyrosol could be synergic to antifungals that target cellular ergosterol. Antifungal susceptibility and drug interaction against planktonic cells were investigated by the broth microdilution method. Tyrosol was able to inhibit planktonic cells, with MIC values ranging from 2.5 to 5.0 mM for both species. Synergism was observed between tyrosol/amphotericin B (11/20 strains), tyrosol/itraconazole (18/20 strains) and tyrosol/fluconazole (18/20 strains). Exogenous tyrosol alone or combined with antifungals at both 10 × MIC and 50 × MIC were able to reduce biofilm of both Candida species. Mature biofilms were susceptible to tyrosol alone at 50 × MIC or combined with amphotericin at both 10 × MIC and 50 × MIC. On the other hand, tyrosol plus azoles at both 10 × MIC and 50 × MIC enhanced biofilm growth.
Exogenous tyrosol destroys Candida albicans and C. tropicalis biofilms.</description><subject>Amphotericin B</subject><subject>Amphotericin B - metabolism</subject><subject>Antifungal agents</subject><subject>Antifungal Agents - metabolism</subject><subject>Azoles</subject><subject>Biofilms</subject><subject>Biofilms - drug effects</subject><subject>Candida</subject><subject>Candida - drug effects</subject><subject>Candida - physiology</subject><subject>Candida albicans</subject><subject>Drug interaction</subject><subject>Drug Synergism</subject><subject>Ergosterol</subject><subject>Fluconazole</subject><subject>Fluconazole - metabolism</subject><subject>Itraconazole</subject><subject>Itraconazole - metabolism</subject><subject>Microbial Sensitivity Tests</subject><subject>Minimum inhibitory concentration</subject><subject>Phenylethyl Alcohol - analogs & derivatives</subject><subject>Phenylethyl Alcohol - metabolism</subject><subject>Planktonic cells</subject><subject>Species</subject><subject>Tyrosol</subject><issn>1567-1356</issn><issn>1567-1364</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqFkUtLxDAUhYMovpduJeDGTTWPSdouZRgfILjRdUnTm5lom9QmFQf88WaoD3Dj6ia5Hyf33IPQCSUXlJT80kAX1sOl8W-Esi20T4XMM8rlbPvnLOQeOgjhmRCaE1Lsoj0m8lJIQffRx-LdL8H5MeC4HnzwLbZuZWsbA-5b5V6id1ZjDW0bsHINrq03tu0C9gbP04NtFA49aAtTH9xKOZ0ucQV2wGEMGvqYBFsb1zj6BEVrRrdUbThCOyYVOP6qh-jpevE4v83uH27u5lf3meZiFjNQNSMKGqlFLjiflXXNeU6VKgUFabTgjLHk2Mic1YXRutDAGq1nOjdGqYIfovNJtx_86wghVp0NG0vKQXJeUVmwouS8ZAk9-4M--3FwabqKpa9ZyaikicomSqeVhQFM1Q-2U8O6oqTaxFJNsVRTLIk__VId6w6aH_o7h98J_dj_o_UJ-mqb6Q</recordid><startdate>20150601</startdate><enddate>20150601</enddate><creator>Cordeiro, Rossana de A.</creator><creator>Teixeira, Carlos E.C.</creator><creator>Brilhante, Raimunda S.N.</creator><creator>Castelo-Branco, Débora S.C.M.</creator><creator>Alencar, Lucas P.</creator><creator>de Oliveira, Jonathas S.</creator><creator>Monteiro, André J.</creator><creator>Bandeira, Tereza J.P.G.</creator><creator>Sidrim, José J.C.</creator><creator>Moreira, José Luciano Bezerra</creator><creator>Rocha, Marcos F.G.</creator><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PHGZM</scope><scope>PHGZT</scope><scope>PJZUB</scope><scope>PKEHL</scope><scope>PPXIY</scope><scope>PQEST</scope><scope>PQGLB</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20150601</creationdate><title>Exogenous tyrosol inhibits planktonic cells and biofilms of Candida species and enhances their susceptibility to antifungals</title><author>Cordeiro, Rossana de A. ; Teixeira, Carlos E.C. ; Brilhante, Raimunda S.N. ; Castelo-Branco, Débora S.C.M. ; Alencar, Lucas P. ; de Oliveira, Jonathas S. ; Monteiro, André J. ; Bandeira, Tereza J.P.G. ; Sidrim, José J.C. ; Moreira, José Luciano Bezerra ; Rocha, Marcos F.G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c354t-eab20aed6c5753349bb3371aa951e6fc53222364f672b8fcc8ce2dcc4c7ffaa83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Amphotericin B</topic><topic>Amphotericin B - 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Academic</collection><jtitle>FEMS yeast research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Cordeiro, Rossana de A.</au><au>Teixeira, Carlos E.C.</au><au>Brilhante, Raimunda S.N.</au><au>Castelo-Branco, Débora S.C.M.</au><au>Alencar, Lucas P.</au><au>de Oliveira, Jonathas S.</au><au>Monteiro, André J.</au><au>Bandeira, Tereza J.P.G.</au><au>Sidrim, José J.C.</au><au>Moreira, José Luciano Bezerra</au><au>Rocha, Marcos F.G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Exogenous tyrosol inhibits planktonic cells and biofilms of Candida species and enhances their susceptibility to antifungals</atitle><jtitle>FEMS yeast research</jtitle><addtitle>FEMS Yeast Res</addtitle><date>2015-06-01</date><risdate>2015</risdate><volume>15</volume><issue>4</issue><spage>fov012</spage><epage>fov012</epage><pages>fov012-fov012</pages><issn>1567-1356</issn><eissn>1567-1364</eissn><abstract>Tyrosol is a quorum-sensing molecule of Candida albicans able to induce hyphal development in the early and intermediate stages of biofilm growth. In the present study, we evaluated the effect of high concentrations of exogenous tyrosol on planktonic cells and biofilms of C. albicans (n = 10) and C. tropicalis (n = 10), and investigated whether tyrosol could be synergic to antifungals that target cellular ergosterol. Antifungal susceptibility and drug interaction against planktonic cells were investigated by the broth microdilution method. Tyrosol was able to inhibit planktonic cells, with MIC values ranging from 2.5 to 5.0 mM for both species. Synergism was observed between tyrosol/amphotericin B (11/20 strains), tyrosol/itraconazole (18/20 strains) and tyrosol/fluconazole (18/20 strains). Exogenous tyrosol alone or combined with antifungals at both 10 × MIC and 50 × MIC were able to reduce biofilm of both Candida species. Mature biofilms were susceptible to tyrosol alone at 50 × MIC or combined with amphotericin at both 10 × MIC and 50 × MIC. On the other hand, tyrosol plus azoles at both 10 × MIC and 50 × MIC enhanced biofilm growth.
Exogenous tyrosol destroys Candida albicans and C. tropicalis biofilms.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>25795651</pmid><doi>10.1093/femsyr/fov012</doi></addata></record> |
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| subjects | Amphotericin B Amphotericin B - metabolism Antifungal agents Antifungal Agents - metabolism Azoles Biofilms Biofilms - drug effects Candida Candida - drug effects Candida - physiology Candida albicans Drug interaction Drug Synergism Ergosterol Fluconazole Fluconazole - metabolism Itraconazole Itraconazole - metabolism Microbial Sensitivity Tests Minimum inhibitory concentration Phenylethyl Alcohol - analogs & derivatives Phenylethyl Alcohol - metabolism Planktonic cells Species Tyrosol |
| title | Exogenous tyrosol inhibits planktonic cells and biofilms of Candida species and enhances their susceptibility to antifungals |
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