Modulation of P-glycoprotein function and multidrug resistance in cancer cells by Thai plant extracts
The effects of ethanol extracts from Thai plants belonging to the families of Annonaceae, Rutaceae, and Zingiberaceae on P-glycoprotein (P-gp) function and multidrug resistance were examined in paclitaxel-resistant HepG2 (PR-HepG2) cells. All the extracts tested, significantly increased the accumula...
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| Veröffentlicht in: | Pharmazie 2014-11, Vol.69 (11), p.823-828 |
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| creator | Takano, M Kakizoe, S Kawami, M Nagai, J Patanasethnont, D Sripanidkulchai, B Yumoto, R |
| description | The effects of ethanol extracts from Thai plants belonging to the families of Annonaceae, Rutaceae, and Zingiberaceae on P-glycoprotein (P-gp) function and multidrug resistance were examined in paclitaxel-resistant HepG2 (PR-HepG2) cells. All the extracts tested, significantly increased
the accumulation of [3H]paclitaxel, a P-gp substrate, in the cells. Among nine extracts, Z01 and Z02, extracts from Curcuma comosa and Kaempferia marginata (Zingiberaceae family), respectively, potently increased the accumulation. In addition, Z01 and Z02 increased
the accumulation of other P-gp substrates, rhodamine 123 and doxorubicin, in PR-HepG2 cells in a concentration-dependent manner. Increased accumulation of rhodamine 123 and doxorubicin by Z01 and Z02 was also confirmed by confocal laser scanning microscopy. The effect of Z01 and Z02 pretreatment
on the expression of MDR1 mRNA was also examined. The expression of MDR1 mRNA was not affected by the treatment of PR-HepG2 cells with these extracts for 48 hours. Cytotoxicity of paclitaxel was examined by XTT and protein assays in the absence and presence of Z02. Z02 potentiated the cytotoxicity
of paclitaxel in PR-HepG2 cells. These results suggest that Curcuma comosa and Kaempferia marginata belonging to Zingiberaceae are useful sources to search for new P-gp modulator(s) that can be used to overcome multidrug resistance of cancer cells. |
| doi_str_mv | 10.1691/ph.2014.4619 |
| format | Article |
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the accumulation of [3H]paclitaxel, a P-gp substrate, in the cells. Among nine extracts, Z01 and Z02, extracts from Curcuma comosa and Kaempferia marginata (Zingiberaceae family), respectively, potently increased the accumulation. In addition, Z01 and Z02 increased
the accumulation of other P-gp substrates, rhodamine 123 and doxorubicin, in PR-HepG2 cells in a concentration-dependent manner. Increased accumulation of rhodamine 123 and doxorubicin by Z01 and Z02 was also confirmed by confocal laser scanning microscopy. The effect of Z01 and Z02 pretreatment
on the expression of MDR1 mRNA was also examined. The expression of MDR1 mRNA was not affected by the treatment of PR-HepG2 cells with these extracts for 48 hours. Cytotoxicity of paclitaxel was examined by XTT and protein assays in the absence and presence of Z02. Z02 potentiated the cytotoxicity
of paclitaxel in PR-HepG2 cells. These results suggest that Curcuma comosa and Kaempferia marginata belonging to Zingiberaceae are useful sources to search for new P-gp modulator(s) that can be used to overcome multidrug resistance of cancer cells.</description><identifier>ISSN: 0031-7144</identifier><identifier>DOI: 10.1691/ph.2014.4619</identifier><identifier>PMID: 25985578</identifier><language>eng</language><publisher>Germany: Govi-Verlag</publisher><subject>Antibiotics, Antineoplastic - metabolism ; Antineoplastic Agents, Phytogenic - pharmacology ; ATP Binding Cassette Transporter, Sub-Family B - biosynthesis ; ATP Binding Cassette Transporter, Sub-Family B - genetics ; ATP-Binding Cassette, Sub-Family B, Member 1 - drug effects ; ATP-Binding Cassette, Sub-Family B, Member 1 - metabolism ; Cell Line, Tumor ; Doxorubicin - metabolism ; Drug Resistance, Multiple - drug effects ; Drug Resistance, Neoplasm - drug effects ; Hep G2 Cells ; Humans ; Paclitaxel - metabolism ; Paclitaxel - pharmacology ; Plant Extracts - pharmacology ; Plants, Medicinal - chemistry ; Thailand</subject><ispartof>Pharmazie, 2014-11, Vol.69 (11), p.823-828</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>288,314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25985578$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Takano, M</creatorcontrib><creatorcontrib>Kakizoe, S</creatorcontrib><creatorcontrib>Kawami, M</creatorcontrib><creatorcontrib>Nagai, J</creatorcontrib><creatorcontrib>Patanasethnont, D</creatorcontrib><creatorcontrib>Sripanidkulchai, B</creatorcontrib><creatorcontrib>Yumoto, R</creatorcontrib><title>Modulation of P-glycoprotein function and multidrug resistance in cancer cells by Thai plant extracts</title><title>Pharmazie</title><addtitle>Pharmazie</addtitle><addtitle>Pharmazie</addtitle><description>The effects of ethanol extracts from Thai plants belonging to the families of Annonaceae, Rutaceae, and Zingiberaceae on P-glycoprotein (P-gp) function and multidrug resistance were examined in paclitaxel-resistant HepG2 (PR-HepG2) cells. All the extracts tested, significantly increased
the accumulation of [3H]paclitaxel, a P-gp substrate, in the cells. Among nine extracts, Z01 and Z02, extracts from Curcuma comosa and Kaempferia marginata (Zingiberaceae family), respectively, potently increased the accumulation. In addition, Z01 and Z02 increased
the accumulation of other P-gp substrates, rhodamine 123 and doxorubicin, in PR-HepG2 cells in a concentration-dependent manner. Increased accumulation of rhodamine 123 and doxorubicin by Z01 and Z02 was also confirmed by confocal laser scanning microscopy. The effect of Z01 and Z02 pretreatment
on the expression of MDR1 mRNA was also examined. The expression of MDR1 mRNA was not affected by the treatment of PR-HepG2 cells with these extracts for 48 hours. Cytotoxicity of paclitaxel was examined by XTT and protein assays in the absence and presence of Z02. Z02 potentiated the cytotoxicity
of paclitaxel in PR-HepG2 cells. These results suggest that Curcuma comosa and Kaempferia marginata belonging to Zingiberaceae are useful sources to search for new P-gp modulator(s) that can be used to overcome multidrug resistance of cancer cells.</description><subject>Antibiotics, Antineoplastic - metabolism</subject><subject>Antineoplastic Agents, Phytogenic - pharmacology</subject><subject>ATP Binding Cassette Transporter, Sub-Family B - biosynthesis</subject><subject>ATP Binding Cassette Transporter, Sub-Family B - genetics</subject><subject>ATP-Binding Cassette, Sub-Family B, Member 1 - drug effects</subject><subject>ATP-Binding Cassette, Sub-Family B, Member 1 - metabolism</subject><subject>Cell Line, Tumor</subject><subject>Doxorubicin - metabolism</subject><subject>Drug Resistance, Multiple - drug effects</subject><subject>Drug Resistance, Neoplasm - drug effects</subject><subject>Hep G2 Cells</subject><subject>Humans</subject><subject>Paclitaxel - metabolism</subject><subject>Paclitaxel - pharmacology</subject><subject>Plant Extracts - pharmacology</subject><subject>Plants, Medicinal - chemistry</subject><subject>Thailand</subject><issn>0031-7144</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kU1v3CAURVm0atK0u64rlt14ysOYgWWVfkqJ0sXs0TPGHiIbu4CjTn59cTJdhgU86V0dHV0I-QBsB1LD5-W44wzETkjQr8glYzVUexDigrxN6Z4xLrlUb8gFb7Rqmr26JO527tYRs58DnXv6uxrGk52XOGfnA-3XYJ9WGDo6rWP2XVwHGl3yKWOwjpaQ3YZIrRvHRNsTPRzR02XEkKn7myPanN6R1z2Oyb0_v1fk8P3b4fpndXP349f1l5vK10LkSgEIJotX3WqhoIUOVYMNsL4R5YamcZ10RRyFYhaYcAhc7y1oxJbb-op8esYW_z-rS9lMPm1eGNy8JgNScc5qrfcl-vEcXdvJdWaJfsJ4Mv-bKYG754APgwsZzf28xlDkjbdmmB-82drdyjUPUgcAwxkHpqA2UMxM53oshZmM0QyPJm3Ery8Qn3DLEeOEj2b7wMLejtTnodAx5m1S9T-2CZNn</recordid><startdate>20141101</startdate><enddate>20141101</enddate><creator>Takano, M</creator><creator>Kakizoe, S</creator><creator>Kawami, M</creator><creator>Nagai, J</creator><creator>Patanasethnont, D</creator><creator>Sripanidkulchai, B</creator><creator>Yumoto, R</creator><general>Govi-Verlag</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20141101</creationdate><title>Modulation of P-glycoprotein function and multidrug resistance in cancer cells by Thai plant extracts</title><author>Takano, M ; Kakizoe, S ; Kawami, M ; Nagai, J ; Patanasethnont, D ; Sripanidkulchai, B ; Yumoto, R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-i344t-8114065783b9481b1da85a510f54510155ed6e557a480c104ea1297c19aab2c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Antibiotics, Antineoplastic - metabolism</topic><topic>Antineoplastic Agents, Phytogenic - pharmacology</topic><topic>ATP Binding Cassette Transporter, Sub-Family B - biosynthesis</topic><topic>ATP Binding Cassette Transporter, Sub-Family B - genetics</topic><topic>ATP-Binding Cassette, Sub-Family B, Member 1 - drug effects</topic><topic>ATP-Binding Cassette, Sub-Family B, Member 1 - metabolism</topic><topic>Cell Line, Tumor</topic><topic>Doxorubicin - metabolism</topic><topic>Drug Resistance, Multiple - drug effects</topic><topic>Drug Resistance, Neoplasm - drug effects</topic><topic>Hep G2 Cells</topic><topic>Humans</topic><topic>Paclitaxel - metabolism</topic><topic>Paclitaxel - pharmacology</topic><topic>Plant Extracts - pharmacology</topic><topic>Plants, Medicinal - chemistry</topic><topic>Thailand</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Takano, M</creatorcontrib><creatorcontrib>Kakizoe, S</creatorcontrib><creatorcontrib>Kawami, M</creatorcontrib><creatorcontrib>Nagai, J</creatorcontrib><creatorcontrib>Patanasethnont, D</creatorcontrib><creatorcontrib>Sripanidkulchai, B</creatorcontrib><creatorcontrib>Yumoto, R</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Pharmazie</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Takano, M</au><au>Kakizoe, S</au><au>Kawami, M</au><au>Nagai, J</au><au>Patanasethnont, D</au><au>Sripanidkulchai, B</au><au>Yumoto, R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Modulation of P-glycoprotein function and multidrug resistance in cancer cells by Thai plant extracts</atitle><jtitle>Pharmazie</jtitle><stitle>Pharmazie</stitle><addtitle>Pharmazie</addtitle><date>2014-11-01</date><risdate>2014</risdate><volume>69</volume><issue>11</issue><spage>823</spage><epage>828</epage><pages>823-828</pages><issn>0031-7144</issn><abstract>The effects of ethanol extracts from Thai plants belonging to the families of Annonaceae, Rutaceae, and Zingiberaceae on P-glycoprotein (P-gp) function and multidrug resistance were examined in paclitaxel-resistant HepG2 (PR-HepG2) cells. All the extracts tested, significantly increased
the accumulation of [3H]paclitaxel, a P-gp substrate, in the cells. Among nine extracts, Z01 and Z02, extracts from Curcuma comosa and Kaempferia marginata (Zingiberaceae family), respectively, potently increased the accumulation. In addition, Z01 and Z02 increased
the accumulation of other P-gp substrates, rhodamine 123 and doxorubicin, in PR-HepG2 cells in a concentration-dependent manner. Increased accumulation of rhodamine 123 and doxorubicin by Z01 and Z02 was also confirmed by confocal laser scanning microscopy. The effect of Z01 and Z02 pretreatment
on the expression of MDR1 mRNA was also examined. The expression of MDR1 mRNA was not affected by the treatment of PR-HepG2 cells with these extracts for 48 hours. Cytotoxicity of paclitaxel was examined by XTT and protein assays in the absence and presence of Z02. Z02 potentiated the cytotoxicity
of paclitaxel in PR-HepG2 cells. These results suggest that Curcuma comosa and Kaempferia marginata belonging to Zingiberaceae are useful sources to search for new P-gp modulator(s) that can be used to overcome multidrug resistance of cancer cells.</abstract><cop>Germany</cop><pub>Govi-Verlag</pub><pmid>25985578</pmid><doi>10.1691/ph.2014.4619</doi><tpages>6</tpages></addata></record> |
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| subjects | Antibiotics, Antineoplastic - metabolism Antineoplastic Agents, Phytogenic - pharmacology ATP Binding Cassette Transporter, Sub-Family B - biosynthesis ATP Binding Cassette Transporter, Sub-Family B - genetics ATP-Binding Cassette, Sub-Family B, Member 1 - drug effects ATP-Binding Cassette, Sub-Family B, Member 1 - metabolism Cell Line, Tumor Doxorubicin - metabolism Drug Resistance, Multiple - drug effects Drug Resistance, Neoplasm - drug effects Hep G2 Cells Humans Paclitaxel - metabolism Paclitaxel - pharmacology Plant Extracts - pharmacology Plants, Medicinal - chemistry Thailand |
| title | Modulation of P-glycoprotein function and multidrug resistance in cancer cells by Thai plant extracts |
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