Impacts on the metabolome of down-regulating polyphenol oxidase in potato tubers
Tubers of potato ( Solanum tuberosum L. cv . Estima) genetically modified to reduce polyphenol oxidase (PPO) activity and enzymatic discolouration were assessed for changes in the metabolome using Liquid Chromatography–Mass Spectrometry (LC–MS) and Gas Chromatography (GC)–MS. Metabolome changes indu...
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| Veröffentlicht in: | Transgenic research 2015-06, Vol.24 (3), p.447-461 |
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| creator | Shepherd, Louise Vida Traill Alexander, Colin James Hackett, Christine Anne McRae, Diane Sungurtas, Julia Anne Verrall, Susan Ramsay Morris, Jennifer Anne Hedley, Peter Edward Rockhold, David Belknap, William Davies, Howard Vivian |
| description | Tubers of potato (
Solanum tuberosum
L.
cv
. Estima) genetically modified to reduce polyphenol oxidase (PPO) activity and enzymatic discolouration were assessed for changes in the metabolome using Liquid Chromatography–Mass Spectrometry (LC–MS) and Gas Chromatography (GC)–MS. Metabolome changes induced over a 48 hour (h) period by tuber wounding (sliced transverse sections) were also assessed using two PPO antisense lines (asPPO) and a wild-type (WT) control. Data were analysed using Principal Components Analysis and Analysis of Variance to assess differences between genotypes and temporal changes post-tuber wounding (by slicing). The levels of 15 metabolites (out of a total of 134 that were detected) differed between the WT and asPPO lines in mature tubers at harvest. A considerably higher number (63) of these metabolites changed significantly over a 48 h period following tuber wounding. For individual metabolites the magnitude of the differences between the WT and asPPO lines at harvest were small compared with the impacts of tuber wounding on metabolite levels. Some of the observed metabolite changes are explicable in terms of pathways known to be affected by wound responses. Whilst some statistically significant interactions (11 metabolites) were observed between line and time after wounding, very few profiles were consistent when comparing the WT with both asPPO lines, and the underlying metabolites appeared to be random in terms of the pathways they occupy. Overall, mechanical damage to tubers has a considerably greater impact on the metabolite profile than any potential unintended effects resulting from the down-regulation of
PPO
gene expression. |
| doi_str_mv | 10.1007/s11248-014-9850-8 |
| format | Article |
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Solanum tuberosum
L.
cv
. Estima) genetically modified to reduce polyphenol oxidase (PPO) activity and enzymatic discolouration were assessed for changes in the metabolome using Liquid Chromatography–Mass Spectrometry (LC–MS) and Gas Chromatography (GC)–MS. Metabolome changes induced over a 48 hour (h) period by tuber wounding (sliced transverse sections) were also assessed using two PPO antisense lines (asPPO) and a wild-type (WT) control. Data were analysed using Principal Components Analysis and Analysis of Variance to assess differences between genotypes and temporal changes post-tuber wounding (by slicing). The levels of 15 metabolites (out of a total of 134 that were detected) differed between the WT and asPPO lines in mature tubers at harvest. A considerably higher number (63) of these metabolites changed significantly over a 48 h period following tuber wounding. For individual metabolites the magnitude of the differences between the WT and asPPO lines at harvest were small compared with the impacts of tuber wounding on metabolite levels. Some of the observed metabolite changes are explicable in terms of pathways known to be affected by wound responses. Whilst some statistically significant interactions (11 metabolites) were observed between line and time after wounding, very few profiles were consistent when comparing the WT with both asPPO lines, and the underlying metabolites appeared to be random in terms of the pathways they occupy. Overall, mechanical damage to tubers has a considerably greater impact on the metabolite profile than any potential unintended effects resulting from the down-regulation of
PPO
gene expression.</description><identifier>ISSN: 0962-8819</identifier><identifier>EISSN: 1573-9368</identifier><identifier>DOI: 10.1007/s11248-014-9850-8</identifier><identifier>PMID: 25417184</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>Analysis of Variance ; Animal Genetics and Genomics ; Biomedical and Life Sciences ; Biomedical Engineering/Biotechnology ; Catechol Oxidase - genetics ; Catechol Oxidase - metabolism ; Chromatography, Liquid - methods ; Color ; Down-Regulation ; Gas Chromatography-Mass Spectrometry ; Gene Expression Regulation, Plant ; Genetic Engineering ; Life Sciences ; Metabolome ; Molecular Medicine ; Oligodeoxyribonucleotides, Antisense ; Original Paper ; Plant Genetics and Genomics ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Plant Tubers - genetics ; Plant Tubers - metabolism ; Plants, Genetically Modified ; Solanum tuberosum - genetics ; Solanum tuberosum - metabolism ; Transgenics</subject><ispartof>Transgenic research, 2015-06, Vol.24 (3), p.447-461</ispartof><rights>Springer International Publishing Switzerland 2014</rights><rights>Springer International Publishing Switzerland 2015</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c442t-873e2e4c2d9bfa8a9103e17c99d022a1f6a49f16d56da0d80d55fc3ccee184103</citedby><cites>FETCH-LOGICAL-c442t-873e2e4c2d9bfa8a9103e17c99d022a1f6a49f16d56da0d80d55fc3ccee184103</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11248-014-9850-8$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11248-014-9850-8$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25417184$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shepherd, Louise Vida Traill</creatorcontrib><creatorcontrib>Alexander, Colin James</creatorcontrib><creatorcontrib>Hackett, Christine Anne</creatorcontrib><creatorcontrib>McRae, Diane</creatorcontrib><creatorcontrib>Sungurtas, Julia Anne</creatorcontrib><creatorcontrib>Verrall, Susan Ramsay</creatorcontrib><creatorcontrib>Morris, Jennifer Anne</creatorcontrib><creatorcontrib>Hedley, Peter Edward</creatorcontrib><creatorcontrib>Rockhold, David</creatorcontrib><creatorcontrib>Belknap, William</creatorcontrib><creatorcontrib>Davies, Howard Vivian</creatorcontrib><title>Impacts on the metabolome of down-regulating polyphenol oxidase in potato tubers</title><title>Transgenic research</title><addtitle>Transgenic Res</addtitle><addtitle>Transgenic Res</addtitle><description>Tubers of potato (
Solanum tuberosum
L.
cv
. Estima) genetically modified to reduce polyphenol oxidase (PPO) activity and enzymatic discolouration were assessed for changes in the metabolome using Liquid Chromatography–Mass Spectrometry (LC–MS) and Gas Chromatography (GC)–MS. Metabolome changes induced over a 48 hour (h) period by tuber wounding (sliced transverse sections) were also assessed using two PPO antisense lines (asPPO) and a wild-type (WT) control. Data were analysed using Principal Components Analysis and Analysis of Variance to assess differences between genotypes and temporal changes post-tuber wounding (by slicing). The levels of 15 metabolites (out of a total of 134 that were detected) differed between the WT and asPPO lines in mature tubers at harvest. A considerably higher number (63) of these metabolites changed significantly over a 48 h period following tuber wounding. For individual metabolites the magnitude of the differences between the WT and asPPO lines at harvest were small compared with the impacts of tuber wounding on metabolite levels. Some of the observed metabolite changes are explicable in terms of pathways known to be affected by wound responses. Whilst some statistically significant interactions (11 metabolites) were observed between line and time after wounding, very few profiles were consistent when comparing the WT with both asPPO lines, and the underlying metabolites appeared to be random in terms of the pathways they occupy. Overall, mechanical damage to tubers has a considerably greater impact on the metabolite profile than any potential unintended effects resulting from the down-regulation of
PPO
gene expression.</description><subject>Analysis of Variance</subject><subject>Animal Genetics and Genomics</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedical Engineering/Biotechnology</subject><subject>Catechol Oxidase - genetics</subject><subject>Catechol Oxidase - metabolism</subject><subject>Chromatography, Liquid - methods</subject><subject>Color</subject><subject>Down-Regulation</subject><subject>Gas Chromatography-Mass Spectrometry</subject><subject>Gene Expression Regulation, Plant</subject><subject>Genetic Engineering</subject><subject>Life Sciences</subject><subject>Metabolome</subject><subject>Molecular Medicine</subject><subject>Oligodeoxyribonucleotides, Antisense</subject><subject>Original Paper</subject><subject>Plant Genetics and Genomics</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Plant Tubers - genetics</subject><subject>Plant Tubers - metabolism</subject><subject>Plants, Genetically Modified</subject><subject>Solanum tuberosum - genetics</subject><subject>Solanum tuberosum - metabolism</subject><subject>Transgenics</subject><issn>0962-8819</issn><issn>1573-9368</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp1kMFq3DAQhkVpaDZJH6CXIOilFzUaWbalYwlpEgg0h-QstNJ418G2XEkmzdtXy25CCfQkGH3_P8NHyBfg34Hz9iIBCKkYB8m0qjlTH8gK6rZiumrUR7LiuhFMKdDH5CSlJ85LSlWfyLGoJbSg5Irc346zdTnRMNG8RTpituswhBFp6KgPzxOLuFkGm_tpQ-cwvMxbnMJAw5_e24S0n8o02xxoXtYY0xk56uyQ8PPhPSWPP68eLm_Y3a_r28sfd8xJKTJTbYUCpRNerzurrAZeIbROa8-FsNA1VuoOGl833nKvuK_rzlXOIZa7C3xKvu175xh-L5iyGfvkcBjshGFJBholBK-E3KFf36FPYYlTuW5HgZSqhqpQsKdcDClF7Mwc-9HGFwPc7HSbvW5TdJudbqNK5vzQvKxH9G-JV78FEHsgla9pg_Gf1f9t_QuvEop7</recordid><startdate>20150601</startdate><enddate>20150601</enddate><creator>Shepherd, Louise Vida Traill</creator><creator>Alexander, Colin James</creator><creator>Hackett, Christine Anne</creator><creator>McRae, Diane</creator><creator>Sungurtas, Julia Anne</creator><creator>Verrall, Susan Ramsay</creator><creator>Morris, Jennifer Anne</creator><creator>Hedley, Peter Edward</creator><creator>Rockhold, David</creator><creator>Belknap, William</creator><creator>Davies, Howard Vivian</creator><general>Springer International Publishing</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TK</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PHGZM</scope><scope>PHGZT</scope><scope>PJZUB</scope><scope>PKEHL</scope><scope>PPXIY</scope><scope>PQEST</scope><scope>PQGLB</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20150601</creationdate><title>Impacts on the metabolome of down-regulating polyphenol oxidase in potato tubers</title><author>Shepherd, Louise Vida Traill ; Alexander, Colin James ; Hackett, Christine Anne ; McRae, Diane ; Sungurtas, Julia Anne ; Verrall, Susan Ramsay ; Morris, Jennifer Anne ; Hedley, Peter Edward ; Rockhold, David ; Belknap, William ; Davies, Howard Vivian</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c442t-873e2e4c2d9bfa8a9103e17c99d022a1f6a49f16d56da0d80d55fc3ccee184103</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Analysis of Variance</topic><topic>Animal Genetics and Genomics</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedical Engineering/Biotechnology</topic><topic>Catechol Oxidase - genetics</topic><topic>Catechol Oxidase - metabolism</topic><topic>Chromatography, Liquid - methods</topic><topic>Color</topic><topic>Down-Regulation</topic><topic>Gas Chromatography-Mass Spectrometry</topic><topic>Gene Expression Regulation, Plant</topic><topic>Genetic Engineering</topic><topic>Life Sciences</topic><topic>Metabolome</topic><topic>Molecular Medicine</topic><topic>Oligodeoxyribonucleotides, Antisense</topic><topic>Original Paper</topic><topic>Plant Genetics and Genomics</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>Plant Tubers - genetics</topic><topic>Plant Tubers - metabolism</topic><topic>Plants, Genetically Modified</topic><topic>Solanum tuberosum - genetics</topic><topic>Solanum tuberosum - metabolism</topic><topic>Transgenics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shepherd, Louise Vida Traill</creatorcontrib><creatorcontrib>Alexander, Colin James</creatorcontrib><creatorcontrib>Hackett, Christine Anne</creatorcontrib><creatorcontrib>McRae, Diane</creatorcontrib><creatorcontrib>Sungurtas, Julia Anne</creatorcontrib><creatorcontrib>Verrall, Susan Ramsay</creatorcontrib><creatorcontrib>Morris, Jennifer Anne</creatorcontrib><creatorcontrib>Hedley, Peter Edward</creatorcontrib><creatorcontrib>Rockhold, David</creatorcontrib><creatorcontrib>Belknap, William</creatorcontrib><creatorcontrib>Davies, Howard Vivian</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>ProQuest Health and Medical</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection (ProQuest)</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest Biological Science Journals</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest Central (New)</collection><collection>ProQuest One Academic (New)</collection><collection>ProQuest Health & Medical Research Collection</collection><collection>ProQuest One Academic Middle East (New)</collection><collection>ProQuest One Health & Nursing</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Applied & Life Sciences</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Transgenic research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shepherd, Louise Vida Traill</au><au>Alexander, Colin James</au><au>Hackett, Christine Anne</au><au>McRae, Diane</au><au>Sungurtas, Julia Anne</au><au>Verrall, Susan Ramsay</au><au>Morris, Jennifer Anne</au><au>Hedley, Peter Edward</au><au>Rockhold, David</au><au>Belknap, William</au><au>Davies, Howard Vivian</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Impacts on the metabolome of down-regulating polyphenol oxidase in potato tubers</atitle><jtitle>Transgenic research</jtitle><stitle>Transgenic Res</stitle><addtitle>Transgenic Res</addtitle><date>2015-06-01</date><risdate>2015</risdate><volume>24</volume><issue>3</issue><spage>447</spage><epage>461</epage><pages>447-461</pages><issn>0962-8819</issn><eissn>1573-9368</eissn><abstract>Tubers of potato (
Solanum tuberosum
L.
cv
. Estima) genetically modified to reduce polyphenol oxidase (PPO) activity and enzymatic discolouration were assessed for changes in the metabolome using Liquid Chromatography–Mass Spectrometry (LC–MS) and Gas Chromatography (GC)–MS. Metabolome changes induced over a 48 hour (h) period by tuber wounding (sliced transverse sections) were also assessed using two PPO antisense lines (asPPO) and a wild-type (WT) control. Data were analysed using Principal Components Analysis and Analysis of Variance to assess differences between genotypes and temporal changes post-tuber wounding (by slicing). The levels of 15 metabolites (out of a total of 134 that were detected) differed between the WT and asPPO lines in mature tubers at harvest. A considerably higher number (63) of these metabolites changed significantly over a 48 h period following tuber wounding. For individual metabolites the magnitude of the differences between the WT and asPPO lines at harvest were small compared with the impacts of tuber wounding on metabolite levels. Some of the observed metabolite changes are explicable in terms of pathways known to be affected by wound responses. Whilst some statistically significant interactions (11 metabolites) were observed between line and time after wounding, very few profiles were consistent when comparing the WT with both asPPO lines, and the underlying metabolites appeared to be random in terms of the pathways they occupy. Overall, mechanical damage to tubers has a considerably greater impact on the metabolite profile than any potential unintended effects resulting from the down-regulation of
PPO
gene expression.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><pmid>25417184</pmid><doi>10.1007/s11248-014-9850-8</doi><tpages>15</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0962-8819 |
| ispartof | Transgenic research, 2015-06, Vol.24 (3), p.447-461 |
| issn | 0962-8819 1573-9368 |
| language | eng |
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| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Analysis of Variance Animal Genetics and Genomics Biomedical and Life Sciences Biomedical Engineering/Biotechnology Catechol Oxidase - genetics Catechol Oxidase - metabolism Chromatography, Liquid - methods Color Down-Regulation Gas Chromatography-Mass Spectrometry Gene Expression Regulation, Plant Genetic Engineering Life Sciences Metabolome Molecular Medicine Oligodeoxyribonucleotides, Antisense Original Paper Plant Genetics and Genomics Plant Proteins - genetics Plant Proteins - metabolism Plant Tubers - genetics Plant Tubers - metabolism Plants, Genetically Modified Solanum tuberosum - genetics Solanum tuberosum - metabolism Transgenics |
| title | Impacts on the metabolome of down-regulating polyphenol oxidase in potato tubers |
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