Effects of Listeria monocytogenes EGD-e and Salmonella enterica ser. Typhimurium LT2 chitinases on intracellular survival in Dictyostelium discoideum and mammalian cell lines
Abstract Some bacterial pathogens produce chitinases as virulence factors during host infection. The molecular target of such enzymes in non-chitinous hosts remains uncertain. We studied the importance of Listeria monocytogenes EGD-e and Salmonella enterica ser. Typhimurium LT2 chitinases for intrac...
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| Veröffentlicht in: | FEMS microbiology letters 2015-05, Vol.362 (10) |
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| creator | Frederiksen, Rikki F. Leisner, Jørgen J. |
| description | Abstract
Some bacterial pathogens produce chitinases as virulence factors during host infection. The molecular target of such enzymes in non-chitinous hosts remains uncertain. We studied the importance of Listeria monocytogenes EGD-e and Salmonella enterica ser. Typhimurium LT2 chitinases for intracellular survival in Dictyostelium discoideum, and for Salmonella, also infection of mammalian cell lines, and a mouse model. The Salmonella chitinase did not contribute significantly to infection of D. discoideum, mammalian cell lines or mice. However, survival in D. discoideum was clearly reduced for Listeria mutants deficient of ChiB (8-fold) or deficient of both ChiA and ChiB (22-fold). Our findings suggest that chitinases from the two species play different roles in virulence.
Listeria chitinases, but not the Salmonella chitinase ChiA, promotes intracellular survival in the cellular slime mould Dictyostelium discoideum.
Graphical Abstract Figure.
Listeria chitinases, but not the Salmonella chitinase ChiA, promotes intracellular survival in the cellular slime mould Dictyostelium discoideum. |
| doi_str_mv | 10.1093/femsle/fnv067 |
| format | Article |
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Some bacterial pathogens produce chitinases as virulence factors during host infection. The molecular target of such enzymes in non-chitinous hosts remains uncertain. We studied the importance of Listeria monocytogenes EGD-e and Salmonella enterica ser. Typhimurium LT2 chitinases for intracellular survival in Dictyostelium discoideum, and for Salmonella, also infection of mammalian cell lines, and a mouse model. The Salmonella chitinase did not contribute significantly to infection of D. discoideum, mammalian cell lines or mice. However, survival in D. discoideum was clearly reduced for Listeria mutants deficient of ChiB (8-fold) or deficient of both ChiA and ChiB (22-fold). Our findings suggest that chitinases from the two species play different roles in virulence.
Listeria chitinases, but not the Salmonella chitinase ChiA, promotes intracellular survival in the cellular slime mould Dictyostelium discoideum.
Graphical Abstract Figure.
Listeria chitinases, but not the Salmonella chitinase ChiA, promotes intracellular survival in the cellular slime mould Dictyostelium discoideum.</description><identifier>ISSN: 0378-1097</identifier><identifier>EISSN: 1574-6968</identifier><identifier>DOI: 10.1093/femsle/fnv067</identifier><identifier>PMID: 25908871</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Animals ; Biotechnology ; Cell Line ; Cell lines ; Chitinase ; Chitinases - genetics ; Chitinases - metabolism ; Dictyostelium - microbiology ; Dictyostelium discoideum ; Gene Expression Regulation, Bacterial ; HeLa Cells ; Humans ; Infections ; Intracellular ; Listeria ; Listeria monocytogenes ; Listeria monocytogenes - enzymology ; Listeria monocytogenes - pathogenicity ; Listeria monocytogenes - physiology ; Mammals ; Mice ; Microbial Viability ; Microbiology ; Salmonella ; Salmonella enterica ; Salmonella Infections, Animal - microbiology ; Salmonella typhimurium - enzymology ; Salmonella typhimurium - pathogenicity ; Salmonella typhimurium - physiology ; Sequence Deletion ; Survival ; Virulence ; Virulence factors ; Virulence Factors - genetics ; Virulence Factors - metabolism</subject><ispartof>FEMS microbiology letters, 2015-05, Vol.362 (10)</ispartof><rights>FEMS 2015. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com 2015</rights><rights>FEMS 2015. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.</rights><rights>FEMS 2015. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c393t-a57f229344e7bc5300e5a362e8ac5da62f8f3a0a2682c7b521c10879d05322dd3</citedby><cites>FETCH-LOGICAL-c393t-a57f229344e7bc5300e5a362e8ac5da62f8f3a0a2682c7b521c10879d05322dd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1578,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25908871$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Frederiksen, Rikki F.</creatorcontrib><creatorcontrib>Leisner, Jørgen J.</creatorcontrib><title>Effects of Listeria monocytogenes EGD-e and Salmonella enterica ser. Typhimurium LT2 chitinases on intracellular survival in Dictyostelium discoideum and mammalian cell lines</title><title>FEMS microbiology letters</title><addtitle>FEMS Microbiol Lett</addtitle><description>Abstract
Some bacterial pathogens produce chitinases as virulence factors during host infection. The molecular target of such enzymes in non-chitinous hosts remains uncertain. We studied the importance of Listeria monocytogenes EGD-e and Salmonella enterica ser. Typhimurium LT2 chitinases for intracellular survival in Dictyostelium discoideum, and for Salmonella, also infection of mammalian cell lines, and a mouse model. The Salmonella chitinase did not contribute significantly to infection of D. discoideum, mammalian cell lines or mice. However, survival in D. discoideum was clearly reduced for Listeria mutants deficient of ChiB (8-fold) or deficient of both ChiA and ChiB (22-fold). Our findings suggest that chitinases from the two species play different roles in virulence.
Listeria chitinases, but not the Salmonella chitinase ChiA, promotes intracellular survival in the cellular slime mould Dictyostelium discoideum.
Graphical Abstract Figure.
Listeria chitinases, but not the Salmonella chitinase ChiA, promotes intracellular survival in the cellular slime mould Dictyostelium discoideum.</description><subject>Animals</subject><subject>Biotechnology</subject><subject>Cell Line</subject><subject>Cell lines</subject><subject>Chitinase</subject><subject>Chitinases - genetics</subject><subject>Chitinases - metabolism</subject><subject>Dictyostelium - microbiology</subject><subject>Dictyostelium discoideum</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>Infections</subject><subject>Intracellular</subject><subject>Listeria</subject><subject>Listeria monocytogenes</subject><subject>Listeria monocytogenes - enzymology</subject><subject>Listeria monocytogenes - pathogenicity</subject><subject>Listeria monocytogenes - physiology</subject><subject>Mammals</subject><subject>Mice</subject><subject>Microbial Viability</subject><subject>Microbiology</subject><subject>Salmonella</subject><subject>Salmonella enterica</subject><subject>Salmonella Infections, Animal - microbiology</subject><subject>Salmonella typhimurium - enzymology</subject><subject>Salmonella typhimurium - pathogenicity</subject><subject>Salmonella typhimurium - physiology</subject><subject>Sequence Deletion</subject><subject>Survival</subject><subject>Virulence</subject><subject>Virulence factors</subject><subject>Virulence Factors - genetics</subject><subject>Virulence Factors - metabolism</subject><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNqFkTtvFDEUha2IKFkCJS2yREMziR9jj6dEyRKQVkrBUo_uemziyI_Fnllp_xS_MR5tAImGypbvd8491kHoHSXXlPT8xppQvLmx8UBkd4ZWVHRtI3upXqEV4Z1qKtVdotelPBFCWkbkBbpkoidKdXSFfq2tNXoqOFm8cWUy2QEOKSZ9nNIPE03B6_u7xmCII_4Gvo6M94BNXFANuJh8jbfH_aMLc3ZzwJstw_rRTS5CqeoUsYtTBl1ls4eMy5wP7gC-PuM7p6djqlv9ohxd0cmNpl6XbQFCAO8g4kWLvath3qBzC76Yty_nFfr-eb29_dJsHu6_3n7aNJr3fGpAdJaxnret6XZacEKMAC6ZUaDFCJJZZTkQYFIx3e0Eo5oS1fUjEZyxceRX6OPJd5_Tz9mUaQg13PLzaNJcBioV7SnjLa3oh3_QpzTnWNMNjBMhBJetqlRzonROpWRjh312AfJxoGRYihxORQ6nIiv__sV13gUz_qF_N_c3YZr3__F6Bn_5rBI</recordid><startdate>20150501</startdate><enddate>20150501</enddate><creator>Frederiksen, Rikki F.</creator><creator>Leisner, Jørgen J.</creator><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20150501</creationdate><title>Effects of Listeria monocytogenes EGD-e and Salmonella enterica ser. Typhimurium LT2 chitinases on intracellular survival in Dictyostelium discoideum and mammalian cell lines</title><author>Frederiksen, Rikki F. ; Leisner, Jørgen J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c393t-a57f229344e7bc5300e5a362e8ac5da62f8f3a0a2682c7b521c10879d05322dd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Animals</topic><topic>Biotechnology</topic><topic>Cell Line</topic><topic>Cell lines</topic><topic>Chitinase</topic><topic>Chitinases - genetics</topic><topic>Chitinases - metabolism</topic><topic>Dictyostelium - microbiology</topic><topic>Dictyostelium discoideum</topic><topic>Gene Expression Regulation, Bacterial</topic><topic>HeLa Cells</topic><topic>Humans</topic><topic>Infections</topic><topic>Intracellular</topic><topic>Listeria</topic><topic>Listeria monocytogenes</topic><topic>Listeria monocytogenes - enzymology</topic><topic>Listeria monocytogenes - pathogenicity</topic><topic>Listeria monocytogenes - physiology</topic><topic>Mammals</topic><topic>Mice</topic><topic>Microbial Viability</topic><topic>Microbiology</topic><topic>Salmonella</topic><topic>Salmonella enterica</topic><topic>Salmonella Infections, Animal - microbiology</topic><topic>Salmonella typhimurium - enzymology</topic><topic>Salmonella typhimurium - pathogenicity</topic><topic>Salmonella typhimurium - physiology</topic><topic>Sequence Deletion</topic><topic>Survival</topic><topic>Virulence</topic><topic>Virulence factors</topic><topic>Virulence Factors - genetics</topic><topic>Virulence Factors - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Frederiksen, Rikki F.</creatorcontrib><creatorcontrib>Leisner, Jørgen J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>FEMS microbiology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Frederiksen, Rikki F.</au><au>Leisner, Jørgen J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of Listeria monocytogenes EGD-e and Salmonella enterica ser. Typhimurium LT2 chitinases on intracellular survival in Dictyostelium discoideum and mammalian cell lines</atitle><jtitle>FEMS microbiology letters</jtitle><addtitle>FEMS Microbiol Lett</addtitle><date>2015-05-01</date><risdate>2015</risdate><volume>362</volume><issue>10</issue><issn>0378-1097</issn><eissn>1574-6968</eissn><abstract>Abstract
Some bacterial pathogens produce chitinases as virulence factors during host infection. The molecular target of such enzymes in non-chitinous hosts remains uncertain. We studied the importance of Listeria monocytogenes EGD-e and Salmonella enterica ser. Typhimurium LT2 chitinases for intracellular survival in Dictyostelium discoideum, and for Salmonella, also infection of mammalian cell lines, and a mouse model. The Salmonella chitinase did not contribute significantly to infection of D. discoideum, mammalian cell lines or mice. However, survival in D. discoideum was clearly reduced for Listeria mutants deficient of ChiB (8-fold) or deficient of both ChiA and ChiB (22-fold). Our findings suggest that chitinases from the two species play different roles in virulence.
Listeria chitinases, but not the Salmonella chitinase ChiA, promotes intracellular survival in the cellular slime mould Dictyostelium discoideum.
Graphical Abstract Figure.
Listeria chitinases, but not the Salmonella chitinase ChiA, promotes intracellular survival in the cellular slime mould Dictyostelium discoideum.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>25908871</pmid><doi>10.1093/femsle/fnv067</doi><oa>free_for_read</oa></addata></record> |
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| source | Oxford University Press Journals All Titles (1996-Current); MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Biotechnology Cell Line Cell lines Chitinase Chitinases - genetics Chitinases - metabolism Dictyostelium - microbiology Dictyostelium discoideum Gene Expression Regulation, Bacterial HeLa Cells Humans Infections Intracellular Listeria Listeria monocytogenes Listeria monocytogenes - enzymology Listeria monocytogenes - pathogenicity Listeria monocytogenes - physiology Mammals Mice Microbial Viability Microbiology Salmonella Salmonella enterica Salmonella Infections, Animal - microbiology Salmonella typhimurium - enzymology Salmonella typhimurium - pathogenicity Salmonella typhimurium - physiology Sequence Deletion Survival Virulence Virulence factors Virulence Factors - genetics Virulence Factors - metabolism |
| title | Effects of Listeria monocytogenes EGD-e and Salmonella enterica ser. Typhimurium LT2 chitinases on intracellular survival in Dictyostelium discoideum and mammalian cell lines |
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