Effect of chemicals on production, composition and antioxidant activity of polysaccharides of Inonotus obliquus
Polysaccharides are important secondary metabolites from the medicinal mushroom Inonotus obliquus. Various fatty acids, surfactants and organic solvents as cell membrane-reorganizing chemicals were investigated for their stimulatory effects on the growth of fungal mycelium and production of exopolys...
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| Veröffentlicht in: | International journal of biological macromolecules 2015-06, Vol.77, p.143-150 |
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| creator | Xu, Xiangqun Quan, Lili Shen, Mengwei |
| description | Polysaccharides are important secondary metabolites from the medicinal mushroom Inonotus obliquus. Various fatty acids, surfactants and organic solvents as cell membrane-reorganizing chemicals were investigated for their stimulatory effects on the growth of fungal mycelium and production of exopolysaccharides (EPS) and endopolysaccharides (IPS) by submerged fermentation of I. obliquus. After evaluation of 14 chemicals, oleic acid, Tween 80, and TritonX-100 were chosen for optimization of addition concentration and addition time. Among the three chemicals, 0.1% (v/v) Tween 80 gave maximum production of mycelial biomass, EPS, IPS1, and IPS2 with a increase of 16.6, 81.6, 37.7 and 18.1%, respectively, when supplemented at the early growth phase (24h after inoculation). These EPS, IPS1, and IPS2 had significantly (p |
| doi_str_mv | 10.1016/j.ijbiomac.2015.03.013 |
| format | Article |
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Various fatty acids, surfactants and organic solvents as cell membrane-reorganizing chemicals were investigated for their stimulatory effects on the growth of fungal mycelium and production of exopolysaccharides (EPS) and endopolysaccharides (IPS) by submerged fermentation of I. obliquus. After evaluation of 14 chemicals, oleic acid, Tween 80, and TritonX-100 were chosen for optimization of addition concentration and addition time. Among the three chemicals, 0.1% (v/v) Tween 80 gave maximum production of mycelial biomass, EPS, IPS1, and IPS2 with a increase of 16.6, 81.6, 37.7 and 18.1%, respectively, when supplemented at the early growth phase (24h after inoculation). These EPS, IPS1, and IPS2 had significantly (p<0.05) stronger scavenging activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals than those from the control medium. IPS1 from Tween 80-containing medium was the most effective antioxidant, with an estimated IC50 value of 0.74mg/mL. This might be attributed to that the EPS and IPS from the Tween 80-containing medium had significantly (p<0.05) higher content of sugar and glucose among the six monosaccharide compositions than those from the control. The simultaneously enhanced accumulation of bioactive EPS and IPS of cultured I. obliquus supplemented with Tween 80 was evident.</description><identifier>ISSN: 0141-8130</identifier><identifier>EISSN: 1879-0003</identifier><identifier>DOI: 10.1016/j.ijbiomac.2015.03.013</identifier><identifier>PMID: 25797403</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Basidiomycota - drug effects ; Basidiomycota - metabolism ; Biphenyl Compounds - chemistry ; Dose-Response Relationship, Drug ; Fatty Acids - pharmacology ; Free Radical Scavengers - chemistry ; Free Radical Scavengers - metabolism ; Free Radical Scavengers - pharmacology ; Inonotus obliquus ; Monosaccharides - analysis ; Picrates - chemistry ; Polysaccharides ; Polysaccharides - biosynthesis ; Polysaccharides - chemistry ; Polysaccharides - pharmacology ; Polysorbates - pharmacology ; Solvents - pharmacology ; Stimulatory agents ; Surface-Active Agents - pharmacology ; Time Factors</subject><ispartof>International journal of biological macromolecules, 2015-06, Vol.77, p.143-150</ispartof><rights>2015 Elsevier B.V.</rights><rights>Copyright © 2015 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c368t-18c9783824f80fcfcb98802f7de7b958f4236f010f9ff6f7b59a1ed8769797d13</citedby><cites>FETCH-LOGICAL-c368t-18c9783824f80fcfcb98802f7de7b958f4236f010f9ff6f7b59a1ed8769797d13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ijbiomac.2015.03.013$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,27929,27930,46000</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25797403$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Xu, Xiangqun</creatorcontrib><creatorcontrib>Quan, Lili</creatorcontrib><creatorcontrib>Shen, Mengwei</creatorcontrib><title>Effect of chemicals on production, composition and antioxidant activity of polysaccharides of Inonotus obliquus</title><title>International journal of biological macromolecules</title><addtitle>Int J Biol Macromol</addtitle><description>Polysaccharides are important secondary metabolites from the medicinal mushroom Inonotus obliquus. Various fatty acids, surfactants and organic solvents as cell membrane-reorganizing chemicals were investigated for their stimulatory effects on the growth of fungal mycelium and production of exopolysaccharides (EPS) and endopolysaccharides (IPS) by submerged fermentation of I. obliquus. After evaluation of 14 chemicals, oleic acid, Tween 80, and TritonX-100 were chosen for optimization of addition concentration and addition time. Among the three chemicals, 0.1% (v/v) Tween 80 gave maximum production of mycelial biomass, EPS, IPS1, and IPS2 with a increase of 16.6, 81.6, 37.7 and 18.1%, respectively, when supplemented at the early growth phase (24h after inoculation). These EPS, IPS1, and IPS2 had significantly (p<0.05) stronger scavenging activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals than those from the control medium. IPS1 from Tween 80-containing medium was the most effective antioxidant, with an estimated IC50 value of 0.74mg/mL. This might be attributed to that the EPS and IPS from the Tween 80-containing medium had significantly (p<0.05) higher content of sugar and glucose among the six monosaccharide compositions than those from the control. The simultaneously enhanced accumulation of bioactive EPS and IPS of cultured I. obliquus supplemented with Tween 80 was evident.</description><subject>Basidiomycota - drug effects</subject><subject>Basidiomycota - metabolism</subject><subject>Biphenyl Compounds - chemistry</subject><subject>Dose-Response Relationship, Drug</subject><subject>Fatty Acids - pharmacology</subject><subject>Free Radical Scavengers - chemistry</subject><subject>Free Radical Scavengers - metabolism</subject><subject>Free Radical Scavengers - pharmacology</subject><subject>Inonotus obliquus</subject><subject>Monosaccharides - analysis</subject><subject>Picrates - chemistry</subject><subject>Polysaccharides</subject><subject>Polysaccharides - biosynthesis</subject><subject>Polysaccharides - chemistry</subject><subject>Polysaccharides - pharmacology</subject><subject>Polysorbates - pharmacology</subject><subject>Solvents - pharmacology</subject><subject>Stimulatory agents</subject><subject>Surface-Active Agents - pharmacology</subject><subject>Time Factors</subject><issn>0141-8130</issn><issn>1879-0003</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMtOwzAQRS0EglL4BZQlCxJm4iaxdyDES6rEBtaW44fqKonbOKno3-OohS0La8ajO3N1DyE3CBkClvfrzK1r51upshywyIBmgPSEzJBVPAUAekpmgAtMGVK4IJchrOO0LJCdk4u8qHi1ADoj_tlao4bE20StTOuUbELiu2TTez2qwfnuLlG-3fjgpk8iOx1fbL-djjWRUbNzw346sPHNPkilVrJ32oRp9N75zg9j7OvGbccxXJEzGy3M9bHOydfL8-fTW7r8eH1_elymipZsSJEpXjHK8oVlYJVVNWcMcltpU9W8YHaR09ICguXWlraqCy7RaFaVPCbTSOfk9nA3BtmOJgyidUGZppGd8WMQWDLkiJzxKC0PUtX7EHpjxaZ3rez3AkFMsMVa_MIWE2wBVETYcfHm6DHWrdF_a790o-DhIDAx6c6ZXgTlTKeMdn2ELrR3_3n8APR4leE</recordid><startdate>20150601</startdate><enddate>20150601</enddate><creator>Xu, Xiangqun</creator><creator>Quan, Lili</creator><creator>Shen, Mengwei</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20150601</creationdate><title>Effect of chemicals on production, composition and antioxidant activity of polysaccharides of Inonotus obliquus</title><author>Xu, Xiangqun ; Quan, Lili ; Shen, Mengwei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c368t-18c9783824f80fcfcb98802f7de7b958f4236f010f9ff6f7b59a1ed8769797d13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Basidiomycota - drug effects</topic><topic>Basidiomycota - metabolism</topic><topic>Biphenyl Compounds - chemistry</topic><topic>Dose-Response Relationship, Drug</topic><topic>Fatty Acids - pharmacology</topic><topic>Free Radical Scavengers - chemistry</topic><topic>Free Radical Scavengers - metabolism</topic><topic>Free Radical Scavengers - pharmacology</topic><topic>Inonotus obliquus</topic><topic>Monosaccharides - analysis</topic><topic>Picrates - chemistry</topic><topic>Polysaccharides</topic><topic>Polysaccharides - biosynthesis</topic><topic>Polysaccharides - chemistry</topic><topic>Polysaccharides - pharmacology</topic><topic>Polysorbates - pharmacology</topic><topic>Solvents - pharmacology</topic><topic>Stimulatory agents</topic><topic>Surface-Active Agents - pharmacology</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xu, Xiangqun</creatorcontrib><creatorcontrib>Quan, Lili</creatorcontrib><creatorcontrib>Shen, Mengwei</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of biological macromolecules</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xu, Xiangqun</au><au>Quan, Lili</au><au>Shen, Mengwei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of chemicals on production, composition and antioxidant activity of polysaccharides of Inonotus obliquus</atitle><jtitle>International journal of biological macromolecules</jtitle><addtitle>Int J Biol Macromol</addtitle><date>2015-06-01</date><risdate>2015</risdate><volume>77</volume><spage>143</spage><epage>150</epage><pages>143-150</pages><issn>0141-8130</issn><eissn>1879-0003</eissn><abstract>Polysaccharides are important secondary metabolites from the medicinal mushroom Inonotus obliquus. Various fatty acids, surfactants and organic solvents as cell membrane-reorganizing chemicals were investigated for their stimulatory effects on the growth of fungal mycelium and production of exopolysaccharides (EPS) and endopolysaccharides (IPS) by submerged fermentation of I. obliquus. After evaluation of 14 chemicals, oleic acid, Tween 80, and TritonX-100 were chosen for optimization of addition concentration and addition time. Among the three chemicals, 0.1% (v/v) Tween 80 gave maximum production of mycelial biomass, EPS, IPS1, and IPS2 with a increase of 16.6, 81.6, 37.7 and 18.1%, respectively, when supplemented at the early growth phase (24h after inoculation). These EPS, IPS1, and IPS2 had significantly (p<0.05) stronger scavenging activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals than those from the control medium. IPS1 from Tween 80-containing medium was the most effective antioxidant, with an estimated IC50 value of 0.74mg/mL. This might be attributed to that the EPS and IPS from the Tween 80-containing medium had significantly (p<0.05) higher content of sugar and glucose among the six monosaccharide compositions than those from the control. The simultaneously enhanced accumulation of bioactive EPS and IPS of cultured I. obliquus supplemented with Tween 80 was evident.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>25797403</pmid><doi>10.1016/j.ijbiomac.2015.03.013</doi><tpages>8</tpages></addata></record> |
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| subjects | Basidiomycota - drug effects Basidiomycota - metabolism Biphenyl Compounds - chemistry Dose-Response Relationship, Drug Fatty Acids - pharmacology Free Radical Scavengers - chemistry Free Radical Scavengers - metabolism Free Radical Scavengers - pharmacology Inonotus obliquus Monosaccharides - analysis Picrates - chemistry Polysaccharides Polysaccharides - biosynthesis Polysaccharides - chemistry Polysaccharides - pharmacology Polysorbates - pharmacology Solvents - pharmacology Stimulatory agents Surface-Active Agents - pharmacology Time Factors |
| title | Effect of chemicals on production, composition and antioxidant activity of polysaccharides of Inonotus obliquus |
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