Expression of both CuZnSOD and APX in chloroplasts enhances tolerance to sulfur dioxide in transgenic sweet potato plants
We have previously reported that transgenic sweet potato (Ipomoea batatas) plants overexpressing both CuZn superoxide dismutase (CuZnSOD) and ascorbate peroxidase (APX) under the control of a stress-inducible SWPA2 promoter in chloroplasts (referred to as SSA plants) showed increased resistance to m...
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| Veröffentlicht in: | Comptes rendus. Biologies 2015-05, Vol.338 (5), p.307-313 |
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| container_title | Comptes rendus. Biologies |
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| creator | Kim, Yun-Hee Lim, Soon Han, Sim-Hee Lee, Jeung Joo Nam, Ki Jung Jeong, Jae Cheol Lee, Haeng-Soon Kwak, Sang-Soo |
| description | We have previously reported that transgenic sweet potato (Ipomoea batatas) plants overexpressing both CuZn superoxide dismutase (CuZnSOD) and ascorbate peroxidase (APX) under the control of a stress-inducible SWPA2 promoter in chloroplasts (referred to as SSA plants) showed increased resistance to methyl viologen-mediated oxidative stress and chilling. To investigate whether SSA plants show enhanced tolerance to air pollutants, they were exposed to 500ppb of sulfur dioxide (SO2). SO2 caused visible damage to the leaves of sweet potato, but damage in the leaves of non-transgenic (NT) plants was more severe than in those of SSA plants. The photosynthetic activity (Fv/Fm) of the SSA plants decreased by only 7% on the 5th day after the treatment, whereas that of NT plants severely decreased by 63% after 5days of recovery. Moreover, the chlorophyll content in the oldest leaf of NT plants decreased by 69%, whereas that of SSA plants remained at a high level. APX activity in NT plants increased about three times under an SO2 stress, and in SSA plants about five times compared to the case with no stress conditions. These results suggest that the overexpression of both CuZnSOD and APX in chloroplasts reduces the oxidative stress derived from SO2. |
| doi_str_mv | 10.1016/j.crvi.2015.03.012 |
| format | Article |
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To investigate whether SSA plants show enhanced tolerance to air pollutants, they were exposed to 500ppb of sulfur dioxide (SO2). SO2 caused visible damage to the leaves of sweet potato, but damage in the leaves of non-transgenic (NT) plants was more severe than in those of SSA plants. The photosynthetic activity (Fv/Fm) of the SSA plants decreased by only 7% on the 5th day after the treatment, whereas that of NT plants severely decreased by 63% after 5days of recovery. Moreover, the chlorophyll content in the oldest leaf of NT plants decreased by 69%, whereas that of SSA plants remained at a high level. APX activity in NT plants increased about three times under an SO2 stress, and in SSA plants about five times compared to the case with no stress conditions. These results suggest that the overexpression of both CuZnSOD and APX in chloroplasts reduces the oxidative stress derived from SO2.</description><identifier>ISSN: 1631-0691</identifier><identifier>ISSN: 1768-3238</identifier><identifier>EISSN: 1768-3238</identifier><identifier>DOI: 10.1016/j.crvi.2015.03.012</identifier><identifier>PMID: 25910434</identifier><language>eng</language><publisher>France: Elsevier SAS</publisher><subject>Air Pollutants - toxicity ; Ascorbate peroxidase ; Ascorbate Peroxidases - biosynthesis ; Chlorophyll - biosynthesis ; Chloroplasts - enzymology ; Gene Expression Regulation, Enzymologic ; Ipomoea batatas - drug effects ; Ipomoea batatas - metabolism ; Oxidative stress ; Oxidative Stress - genetics ; Photosynthesis - drug effects ; Plant Leaves - growth & development ; Plant Leaves - metabolism ; Plants, Genetically Modified - genetics ; Stress-inducible promoter ; Sulfur dioxide ; Sulfur Dioxide - toxicity ; Superoxide dismutase ; Superoxide Dismutase - biosynthesis ; Sweet potato</subject><ispartof>Comptes rendus. Biologies, 2015-05, Vol.338 (5), p.307-313</ispartof><rights>2015 Académie des sciences</rights><rights>Copyright © 2015 Académie des sciences. Published by Elsevier SAS. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c426t-618e5dcb32ca502b57d69f22adf5f4b31280b969bcb4ce052e62cdeba6243cb83</citedby><cites>FETCH-LOGICAL-c426t-618e5dcb32ca502b57d69f22adf5f4b31280b969bcb4ce052e62cdeba6243cb83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.crvi.2015.03.012$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25910434$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, Yun-Hee</creatorcontrib><creatorcontrib>Lim, Soon</creatorcontrib><creatorcontrib>Han, Sim-Hee</creatorcontrib><creatorcontrib>Lee, Jeung Joo</creatorcontrib><creatorcontrib>Nam, Ki Jung</creatorcontrib><creatorcontrib>Jeong, Jae Cheol</creatorcontrib><creatorcontrib>Lee, Haeng-Soon</creatorcontrib><creatorcontrib>Kwak, Sang-Soo</creatorcontrib><title>Expression of both CuZnSOD and APX in chloroplasts enhances tolerance to sulfur dioxide in transgenic sweet potato plants</title><title>Comptes rendus. Biologies</title><addtitle>C R Biol</addtitle><description>We have previously reported that transgenic sweet potato (Ipomoea batatas) plants overexpressing both CuZn superoxide dismutase (CuZnSOD) and ascorbate peroxidase (APX) under the control of a stress-inducible SWPA2 promoter in chloroplasts (referred to as SSA plants) showed increased resistance to methyl viologen-mediated oxidative stress and chilling. To investigate whether SSA plants show enhanced tolerance to air pollutants, they were exposed to 500ppb of sulfur dioxide (SO2). SO2 caused visible damage to the leaves of sweet potato, but damage in the leaves of non-transgenic (NT) plants was more severe than in those of SSA plants. The photosynthetic activity (Fv/Fm) of the SSA plants decreased by only 7% on the 5th day after the treatment, whereas that of NT plants severely decreased by 63% after 5days of recovery. Moreover, the chlorophyll content in the oldest leaf of NT plants decreased by 69%, whereas that of SSA plants remained at a high level. APX activity in NT plants increased about three times under an SO2 stress, and in SSA plants about five times compared to the case with no stress conditions. These results suggest that the overexpression of both CuZnSOD and APX in chloroplasts reduces the oxidative stress derived from SO2.</description><subject>Air Pollutants - toxicity</subject><subject>Ascorbate peroxidase</subject><subject>Ascorbate Peroxidases - biosynthesis</subject><subject>Chlorophyll - biosynthesis</subject><subject>Chloroplasts - enzymology</subject><subject>Gene Expression Regulation, Enzymologic</subject><subject>Ipomoea batatas - drug effects</subject><subject>Ipomoea batatas - metabolism</subject><subject>Oxidative stress</subject><subject>Oxidative Stress - genetics</subject><subject>Photosynthesis - drug effects</subject><subject>Plant Leaves - growth & development</subject><subject>Plant Leaves - metabolism</subject><subject>Plants, Genetically Modified - genetics</subject><subject>Stress-inducible promoter</subject><subject>Sulfur dioxide</subject><subject>Sulfur Dioxide - toxicity</subject><subject>Superoxide dismutase</subject><subject>Superoxide Dismutase - biosynthesis</subject><subject>Sweet potato</subject><issn>1631-0691</issn><issn>1768-3238</issn><issn>1768-3238</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEtv1DAUhS0EoqXwB1ggL9kk-BF7EolNNZSHVKlILRJiY8X2DeNRJg6-Th__HkfTsmTlI_k7R_ZHyFvOas64_rCvXboNtWBc1UzWjItn5JRvdFtJIdvnJWvJK6Y7fkJeIe5ZKXVKvSQnQnWcNbI5JQ8X93MCxBAnGgdqY97R7fJrur76RPvJ0_PvP2mYqNuNMcV57DEjhWnXTw6Q5jhCWmNJFJdxWBL1Id4HD2splzv8DVNwFO8AMp1j7gtZZqaMr8mLoR8R3jyeZ-TH54ub7dfq8urLt-35ZeUaoXOleQvKOyuF6xUTVm287gYhej-oobGSi5bZTnfW2cYBUwK0cB5sr0UjnW3lGXl_3J1T_LMAZnMI6GAsj4C4oOG65R1rtdgUVBxRlyJigsHMKRz69GA4M6tyszercrMqN0yaoryU3j3uL_YA_l_lyXEBPh4BKL-8DZAMugDFmg8JXDY-hv_t_wUE45Sm</recordid><startdate>20150501</startdate><enddate>20150501</enddate><creator>Kim, Yun-Hee</creator><creator>Lim, Soon</creator><creator>Han, Sim-Hee</creator><creator>Lee, Jeung Joo</creator><creator>Nam, Ki Jung</creator><creator>Jeong, Jae Cheol</creator><creator>Lee, Haeng-Soon</creator><creator>Kwak, Sang-Soo</creator><general>Elsevier SAS</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20150501</creationdate><title>Expression of both CuZnSOD and APX in chloroplasts enhances tolerance to sulfur dioxide in transgenic sweet potato plants</title><author>Kim, Yun-Hee ; Lim, Soon ; Han, Sim-Hee ; Lee, Jeung Joo ; Nam, Ki Jung ; Jeong, Jae Cheol ; Lee, Haeng-Soon ; Kwak, Sang-Soo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c426t-618e5dcb32ca502b57d69f22adf5f4b31280b969bcb4ce052e62cdeba6243cb83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Air Pollutants - toxicity</topic><topic>Ascorbate peroxidase</topic><topic>Ascorbate Peroxidases - biosynthesis</topic><topic>Chlorophyll - biosynthesis</topic><topic>Chloroplasts - enzymology</topic><topic>Gene Expression Regulation, Enzymologic</topic><topic>Ipomoea batatas - drug effects</topic><topic>Ipomoea batatas - metabolism</topic><topic>Oxidative stress</topic><topic>Oxidative Stress - genetics</topic><topic>Photosynthesis - drug effects</topic><topic>Plant Leaves - growth & development</topic><topic>Plant Leaves - metabolism</topic><topic>Plants, Genetically Modified - genetics</topic><topic>Stress-inducible promoter</topic><topic>Sulfur dioxide</topic><topic>Sulfur Dioxide - toxicity</topic><topic>Superoxide dismutase</topic><topic>Superoxide Dismutase - biosynthesis</topic><topic>Sweet potato</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, Yun-Hee</creatorcontrib><creatorcontrib>Lim, Soon</creatorcontrib><creatorcontrib>Han, Sim-Hee</creatorcontrib><creatorcontrib>Lee, Jeung Joo</creatorcontrib><creatorcontrib>Nam, Ki Jung</creatorcontrib><creatorcontrib>Jeong, Jae Cheol</creatorcontrib><creatorcontrib>Lee, Haeng-Soon</creatorcontrib><creatorcontrib>Kwak, Sang-Soo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Comptes rendus. Biologies</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, Yun-Hee</au><au>Lim, Soon</au><au>Han, Sim-Hee</au><au>Lee, Jeung Joo</au><au>Nam, Ki Jung</au><au>Jeong, Jae Cheol</au><au>Lee, Haeng-Soon</au><au>Kwak, Sang-Soo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of both CuZnSOD and APX in chloroplasts enhances tolerance to sulfur dioxide in transgenic sweet potato plants</atitle><jtitle>Comptes rendus. Biologies</jtitle><addtitle>C R Biol</addtitle><date>2015-05-01</date><risdate>2015</risdate><volume>338</volume><issue>5</issue><spage>307</spage><epage>313</epage><pages>307-313</pages><issn>1631-0691</issn><issn>1768-3238</issn><eissn>1768-3238</eissn><abstract>We have previously reported that transgenic sweet potato (Ipomoea batatas) plants overexpressing both CuZn superoxide dismutase (CuZnSOD) and ascorbate peroxidase (APX) under the control of a stress-inducible SWPA2 promoter in chloroplasts (referred to as SSA plants) showed increased resistance to methyl viologen-mediated oxidative stress and chilling. To investigate whether SSA plants show enhanced tolerance to air pollutants, they were exposed to 500ppb of sulfur dioxide (SO2). SO2 caused visible damage to the leaves of sweet potato, but damage in the leaves of non-transgenic (NT) plants was more severe than in those of SSA plants. The photosynthetic activity (Fv/Fm) of the SSA plants decreased by only 7% on the 5th day after the treatment, whereas that of NT plants severely decreased by 63% after 5days of recovery. Moreover, the chlorophyll content in the oldest leaf of NT plants decreased by 69%, whereas that of SSA plants remained at a high level. APX activity in NT plants increased about three times under an SO2 stress, and in SSA plants about five times compared to the case with no stress conditions. These results suggest that the overexpression of both CuZnSOD and APX in chloroplasts reduces the oxidative stress derived from SO2.</abstract><cop>France</cop><pub>Elsevier SAS</pub><pmid>25910434</pmid><doi>10.1016/j.crvi.2015.03.012</doi><tpages>7</tpages></addata></record> |
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| subjects | Air Pollutants - toxicity Ascorbate peroxidase Ascorbate Peroxidases - biosynthesis Chlorophyll - biosynthesis Chloroplasts - enzymology Gene Expression Regulation, Enzymologic Ipomoea batatas - drug effects Ipomoea batatas - metabolism Oxidative stress Oxidative Stress - genetics Photosynthesis - drug effects Plant Leaves - growth & development Plant Leaves - metabolism Plants, Genetically Modified - genetics Stress-inducible promoter Sulfur dioxide Sulfur Dioxide - toxicity Superoxide dismutase Superoxide Dismutase - biosynthesis Sweet potato |
| title | Expression of both CuZnSOD and APX in chloroplasts enhances tolerance to sulfur dioxide in transgenic sweet potato plants |
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