Determination of recognition nucleotides for Escherichia coli phenylalanyl-tRNA synthetase

The nucleotides in Escherichia coli tRNA(Phe) required for recognition by its cognate synthetase have been determined in vitro by measuring the kinetic parameters for aminoacylation using mutant tRNA(Phe) transcripts with purified E. coli tRNA(Phe) synthetase. The substitution of 11 nucleotides in E...

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Veröffentlicht in:	Biochemistry (Easton) 1992-10, Vol.31 (42), p.10380-10389
Hauptverfasser:	Peterson, Ellen Tinkle, Uhlenbeck, Olke C
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Base Sequence Biological and medical sciences Cloning, Molecular Enzymes and enzyme inhibitors Escherichia coli Escherichia coli - enzymology Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Kinetics Ligases Molecular Sequence Data Mutagenesis, Site-Directed Nucleic Acid Conformation Oligodeoxyribonucleotides Phenylalanine-tRNA Ligase - genetics Phenylalanine-tRNA Ligase - isolation & purification Phenylalanine-tRNA Ligase - metabolism Polymerase Chain Reaction - methods Recombinant Proteins - isolation & purification Recombinant Proteins - metabolism RNA, Transfer, Phe - genetics RNA, Transfer, Phe - metabolism Substrate Specificity Transcription, Genetic
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