Conventional karyotyping and fluorescence in situ hybridization: an effective utilization strategy in diagnostic adult acute myeloid leukemia
Cytogenetics defines disease entities and predicts prognosis in acute myeloid leukemia (AML). Conventional karyotyping provides a comprehensive view of the genome, while fluorescence in situ hybridization (FISH) detects targeted abnormalities. The aim of this study was to compare the utility of kary...
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| Veröffentlicht in: | American journal of clinical pathology 2015-06, Vol.143 (6), p.873-878 |
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| container_title | American journal of clinical pathology |
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| creator | He, Rong Wiktor, Anne E Hanson, Curtis A Ketterling, Rhett P Kurtin, Paul J Van Dyke, Daniel L Litzow, Mark R Howard, Matthew T Howard, Matthew H Reichard, Kaaren K |
| description | Cytogenetics defines disease entities and predicts prognosis in acute myeloid leukemia (AML). Conventional karyotyping provides a comprehensive view of the genome, while fluorescence in situ hybridization (FISH) detects targeted abnormalities. The aim of this study was to compare the utility of karyotyping and FISH in adult AML.
We studied 250 adult AML cases with concurrent karyotyping and FISH testing. Karyotyping was considered adequate when 20 or more metaphases were analyzed.
In total, 220 cases had adequate karyotyping and were classified as normal karyotype/normal FISH (n = 92), normal karyotype/abnormal FISH (n = 4), abnormal karyotype/normal FISH (n = 8), and abnormal karyotype/abnormal FISH (n = 116). The overall karyotype/FISH concordance rate was 97.7% with five discordant cases identified, four from the normal karyotype/abnormal FISH group and one from the abnormal karyotype/abnormal FISH group. No karyotype/FISH discordance was seen in the abnormal karyotype/normal FISH group for the FISH probes evaluated. FISH lent prognostic information in one (0.5%) of 220 cases with normal karyotype/abnormal FISH: CBFB-MYH11 fusion, indicating favorable prognosis.
In adult AML, FISH rarely provides additional information when karyotyping is adequate. We therefore propose an evidence-based, cost-effective algorithmic approach for routine conventional karyotype and FISH testing in adult AML workup. |
| doi_str_mv | 10.1309/AJCPP6LVMQG4LNCK |
| format | Article |
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We studied 250 adult AML cases with concurrent karyotyping and FISH testing. Karyotyping was considered adequate when 20 or more metaphases were analyzed.
In total, 220 cases had adequate karyotyping and were classified as normal karyotype/normal FISH (n = 92), normal karyotype/abnormal FISH (n = 4), abnormal karyotype/normal FISH (n = 8), and abnormal karyotype/abnormal FISH (n = 116). The overall karyotype/FISH concordance rate was 97.7% with five discordant cases identified, four from the normal karyotype/abnormal FISH group and one from the abnormal karyotype/abnormal FISH group. No karyotype/FISH discordance was seen in the abnormal karyotype/normal FISH group for the FISH probes evaluated. FISH lent prognostic information in one (0.5%) of 220 cases with normal karyotype/abnormal FISH: CBFB-MYH11 fusion, indicating favorable prognosis.
In adult AML, FISH rarely provides additional information when karyotyping is adequate. We therefore propose an evidence-based, cost-effective algorithmic approach for routine conventional karyotype and FISH testing in adult AML workup.</description><identifier>ISSN: 0002-9173</identifier><identifier>EISSN: 1943-7722</identifier><identifier>DOI: 10.1309/AJCPP6LVMQG4LNCK</identifier><identifier>PMID: 25972330</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Adult ; Algorithms ; Female ; Humans ; In Situ Hybridization, Fluorescence - methods ; Karyotyping - methods ; Leukemia, Myeloid, Acute - genetics ; Male</subject><ispartof>American journal of clinical pathology, 2015-06, Vol.143 (6), p.873-878</ispartof><rights>Copyright© by the American Society for Clinical Pathology.</rights><rights>Copyright American Society for Clinical Pathology Jun 2015</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25972330$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>He, Rong</creatorcontrib><creatorcontrib>Wiktor, Anne E</creatorcontrib><creatorcontrib>Hanson, Curtis A</creatorcontrib><creatorcontrib>Ketterling, Rhett P</creatorcontrib><creatorcontrib>Kurtin, Paul J</creatorcontrib><creatorcontrib>Van Dyke, Daniel L</creatorcontrib><creatorcontrib>Litzow, Mark R</creatorcontrib><creatorcontrib>Howard, Matthew T</creatorcontrib><creatorcontrib>Howard, Matthew H</creatorcontrib><creatorcontrib>Reichard, Kaaren K</creatorcontrib><title>Conventional karyotyping and fluorescence in situ hybridization: an effective utilization strategy in diagnostic adult acute myeloid leukemia</title><title>American journal of clinical pathology</title><addtitle>Am J Clin Pathol</addtitle><description>Cytogenetics defines disease entities and predicts prognosis in acute myeloid leukemia (AML). Conventional karyotyping provides a comprehensive view of the genome, while fluorescence in situ hybridization (FISH) detects targeted abnormalities. The aim of this study was to compare the utility of karyotyping and FISH in adult AML.
We studied 250 adult AML cases with concurrent karyotyping and FISH testing. Karyotyping was considered adequate when 20 or more metaphases were analyzed.
In total, 220 cases had adequate karyotyping and were classified as normal karyotype/normal FISH (n = 92), normal karyotype/abnormal FISH (n = 4), abnormal karyotype/normal FISH (n = 8), and abnormal karyotype/abnormal FISH (n = 116). The overall karyotype/FISH concordance rate was 97.7% with five discordant cases identified, four from the normal karyotype/abnormal FISH group and one from the abnormal karyotype/abnormal FISH group. No karyotype/FISH discordance was seen in the abnormal karyotype/normal FISH group for the FISH probes evaluated. FISH lent prognostic information in one (0.5%) of 220 cases with normal karyotype/abnormal FISH: CBFB-MYH11 fusion, indicating favorable prognosis.
In adult AML, FISH rarely provides additional information when karyotyping is adequate. We therefore propose an evidence-based, cost-effective algorithmic approach for routine conventional karyotype and FISH testing in adult AML workup.</description><subject>Adult</subject><subject>Algorithms</subject><subject>Female</subject><subject>Humans</subject><subject>In Situ Hybridization, Fluorescence - methods</subject><subject>Karyotyping - methods</subject><subject>Leukemia, Myeloid, Acute - genetics</subject><subject>Male</subject><issn>0002-9173</issn><issn>1943-7722</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpd0E1P3DAQBmALUZUt9M4JWeLCJa0_EjvmhiK6_dhSkBDXlZNMFu869hLbK4X_0P9MVmwvPc1hnnekeRE6p-QL5UR9vflZ3d-LxdPvh3m-uKt-HaEZVTnPpGTsGM0IISxTVPIT9CmENSGUlST_iE5YoSTjnMzQ38q7HbhovNMWb_Qw-jhujVth7Vrc2eQHCA24BrBxOJiY8PNYD6Y1r3ofup4chq6DJpod4BSNPWxwiIOOsBr3wdbolfMhmgbrNtmIdZMi4H4E602LLaQN9EafoQ-dtgE-H-Ypevx2-1h9zxZ_5j-qm0W2ZVzFDDQo3hSlBNZJKgopa1UXDIQiUOZtW-uyaHjZMSF5rkkJTVcIJRTtpOZE8FN09X52O_iXBCEuezM9aa124FNYUlFSJkTO8ole_kfXPg1TV3uluKRMKTWpi4NKdQ_tcjuYfqpy-a9n_gYIEIPG</recordid><startdate>201506</startdate><enddate>201506</enddate><creator>He, Rong</creator><creator>Wiktor, Anne E</creator><creator>Hanson, Curtis A</creator><creator>Ketterling, Rhett P</creator><creator>Kurtin, Paul J</creator><creator>Van Dyke, Daniel L</creator><creator>Litzow, Mark R</creator><creator>Howard, Matthew T</creator><creator>Howard, Matthew H</creator><creator>Reichard, Kaaren K</creator><general>Oxford University Press</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>3V.</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope></search><sort><creationdate>201506</creationdate><title>Conventional karyotyping and fluorescence in situ hybridization: an effective utilization strategy in diagnostic adult acute myeloid leukemia</title><author>He, Rong ; Wiktor, Anne E ; Hanson, Curtis A ; Ketterling, Rhett P ; Kurtin, Paul J ; Van Dyke, Daniel L ; Litzow, Mark R ; Howard, Matthew T ; Howard, Matthew H ; Reichard, Kaaren K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p239t-eae93c587e2f716577b9b52e690e84ddba85c38f26734a08ecf569691f7a3063</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Adult</topic><topic>Algorithms</topic><topic>Female</topic><topic>Humans</topic><topic>In Situ Hybridization, Fluorescence - methods</topic><topic>Karyotyping - methods</topic><topic>Leukemia, Myeloid, Acute - genetics</topic><topic>Male</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>He, Rong</creatorcontrib><creatorcontrib>Wiktor, Anne E</creatorcontrib><creatorcontrib>Hanson, Curtis A</creatorcontrib><creatorcontrib>Ketterling, Rhett P</creatorcontrib><creatorcontrib>Kurtin, Paul J</creatorcontrib><creatorcontrib>Van Dyke, Daniel L</creatorcontrib><creatorcontrib>Litzow, Mark R</creatorcontrib><creatorcontrib>Howard, Matthew T</creatorcontrib><creatorcontrib>Howard, Matthew H</creatorcontrib><creatorcontrib>Reichard, Kaaren K</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><jtitle>American journal of clinical pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>He, Rong</au><au>Wiktor, Anne E</au><au>Hanson, Curtis A</au><au>Ketterling, Rhett P</au><au>Kurtin, Paul J</au><au>Van Dyke, Daniel L</au><au>Litzow, Mark R</au><au>Howard, Matthew T</au><au>Howard, Matthew H</au><au>Reichard, Kaaren K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Conventional karyotyping and fluorescence in situ hybridization: an effective utilization strategy in diagnostic adult acute myeloid leukemia</atitle><jtitle>American journal of clinical pathology</jtitle><addtitle>Am J Clin Pathol</addtitle><date>2015-06</date><risdate>2015</risdate><volume>143</volume><issue>6</issue><spage>873</spage><epage>878</epage><pages>873-878</pages><issn>0002-9173</issn><eissn>1943-7722</eissn><abstract>Cytogenetics defines disease entities and predicts prognosis in acute myeloid leukemia (AML). Conventional karyotyping provides a comprehensive view of the genome, while fluorescence in situ hybridization (FISH) detects targeted abnormalities. The aim of this study was to compare the utility of karyotyping and FISH in adult AML.
We studied 250 adult AML cases with concurrent karyotyping and FISH testing. Karyotyping was considered adequate when 20 or more metaphases were analyzed.
In total, 220 cases had adequate karyotyping and were classified as normal karyotype/normal FISH (n = 92), normal karyotype/abnormal FISH (n = 4), abnormal karyotype/normal FISH (n = 8), and abnormal karyotype/abnormal FISH (n = 116). The overall karyotype/FISH concordance rate was 97.7% with five discordant cases identified, four from the normal karyotype/abnormal FISH group and one from the abnormal karyotype/abnormal FISH group. No karyotype/FISH discordance was seen in the abnormal karyotype/normal FISH group for the FISH probes evaluated. FISH lent prognostic information in one (0.5%) of 220 cases with normal karyotype/abnormal FISH: CBFB-MYH11 fusion, indicating favorable prognosis.
In adult AML, FISH rarely provides additional information when karyotyping is adequate. We therefore propose an evidence-based, cost-effective algorithmic approach for routine conventional karyotype and FISH testing in adult AML workup.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>25972330</pmid><doi>10.1309/AJCPP6LVMQG4LNCK</doi><tpages>6</tpages></addata></record> |
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| ispartof | American journal of clinical pathology, 2015-06, Vol.143 (6), p.873-878 |
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| language | eng |
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| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current); EZB-FREE-00999 freely available EZB journals |
| subjects | Adult Algorithms Female Humans In Situ Hybridization, Fluorescence - methods Karyotyping - methods Leukemia, Myeloid, Acute - genetics Male |
| title | Conventional karyotyping and fluorescence in situ hybridization: an effective utilization strategy in diagnostic adult acute myeloid leukemia |
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