Engineering the oxyanion hole of trypsin for promoting the reverse of proteolysis

Although proteases are capable of synthesizing peptide bonds via the reverse of proteolysis, they are not proficient at peptide fragment ligation. Further manipulations are needed to shift the native enzyme activity from the cleavage to the synthesis of peptides especially when longer peptides or ev...

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Veröffentlicht in:	Journal of peptide science 2014-02, Vol.20 (2), p.128-136
Hauptverfasser:	Franke, Lars, Liebscher, Sandra, Bordusa, Frank
Format:	Artikel
Sprache:	eng
Schlagworte:	biocatalysis Chromatography, High Pressure Liquid Electrophoresis, Polyacrylamide Gel enzymatic peptide synthesis enzyme engineering Enzymes Peptides Protein Engineering protein modification Proteolysis trypsin Trypsin - chemistry Trypsin - genetics
Online-Zugang:	Volltext
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