EGFR/ERBB receptors differentially modulate sebaceous lipogenesis
•EGFR, ERBB2 and ERBB3 tyrosine kinase receptors are expressed in human sebocytes.•Expression of EGFR/ERBBs is downregulated during sebaceous lipogenesis.•Downregulation of EGFR and ERBB3 using siRNAs increases lipogenesis.•EGFR/ERBB downregulation differentially modulates the expression of lipogeni...
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| Veröffentlicht in: | FEBS letters 2015-05, Vol.589 (12), p.1376-1382 |
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| creator | Dahlhoff, Maik Camera, Emanuela Ludovici, Matteo Picardo, Mauro Müller, Udo Leonhardt, Heinrich Zouboulis, Christos C. Schneider, Marlon R. |
| description | •EGFR, ERBB2 and ERBB3 tyrosine kinase receptors are expressed in human sebocytes.•Expression of EGFR/ERBBs is downregulated during sebaceous lipogenesis.•Downregulation of EGFR and ERBB3 using siRNAs increases lipogenesis.•EGFR/ERBB downregulation differentially modulates the expression of lipogenic markers.
The roles of the epidermal growth factor receptor (EGFR) in sebaceous glands remain poorly explored. We show that human sebocytes express EGFR and lower levels of ERBB2 and ERBB3, all receptors being downregulated after the induction of lipid synthesis. Nile red staining showed that siRNA-mediated downregulation of EGFR or ERBB3 increases lipid accumulation, whereas ERBB2 downregulation has no effect. Spectrometry confirmed induction of triglycerides after EGFR or ERBB3 downregulation and revealed induction of cholesteryl esters after downregulation of EGFR, ERBB2 or ERBB3. Thus, EGFR/ERBB receptors differentially modulate sebaceous lipogenesis, a key feature of sebaceous gland physiology and of several skin diseases. |
| doi_str_mv | 10.1016/j.febslet.2015.04.003 |
| format | Article |
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The roles of the epidermal growth factor receptor (EGFR) in sebaceous glands remain poorly explored. We show that human sebocytes express EGFR and lower levels of ERBB2 and ERBB3, all receptors being downregulated after the induction of lipid synthesis. Nile red staining showed that siRNA-mediated downregulation of EGFR or ERBB3 increases lipid accumulation, whereas ERBB2 downregulation has no effect. Spectrometry confirmed induction of triglycerides after EGFR or ERBB3 downregulation and revealed induction of cholesteryl esters after downregulation of EGFR, ERBB2 or ERBB3. Thus, EGFR/ERBB receptors differentially modulate sebaceous lipogenesis, a key feature of sebaceous gland physiology and of several skin diseases.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/j.febslet.2015.04.003</identifier><identifier>PMID: 25889637</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>Biomarkers - metabolism ; Cell Line ; Cholesterol Esters - metabolism ; Down-Regulation ; Epidermal growth factor receptor ; ERBB ; Fatty Acids, Nonesterified - metabolism ; Gene Expression Regulation, Enzymologic ; Humans ; Ligands ; Linoleic Acid - metabolism ; Lipogenesis ; Receptor, Epidermal Growth Factor - antagonists & inhibitors ; Receptor, Epidermal Growth Factor - genetics ; Receptor, Epidermal Growth Factor - metabolism ; Receptor, ErbB-2 - antagonists & inhibitors ; Receptor, ErbB-2 - genetics ; Receptor, ErbB-2 - metabolism ; Receptor, ErbB-3 - antagonists & inhibitors ; Receptor, ErbB-3 - genetics ; Receptor, ErbB-3 - metabolism ; RNA Interference ; RNA, Small Interfering ; Sebaceous gland ; Sebaceous Glands - enzymology ; Sebaceous Glands - metabolism ; Sebaceous lipogenesis ; Triglycerides - metabolism ; Up-Regulation</subject><ispartof>FEBS letters, 2015-05, Vol.589 (12), p.1376-1382</ispartof><rights>2015 Federation of European Biochemical Societies</rights><rights>FEBS Letters 589 (2015) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><rights>Copyright © 2015 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5479-3e8dad3bc7eb56eb0f719a58f6509759a2970835ddcfb6aef2d81346ba28f3203</citedby><cites>FETCH-LOGICAL-c5479-3e8dad3bc7eb56eb0f719a58f6509759a2970835ddcfb6aef2d81346ba28f3203</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1016%2Fj.febslet.2015.04.003$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0014579315002409$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,1411,1427,3537,27901,27902,45550,45551,46384,46808,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25889637$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Dahlhoff, Maik</creatorcontrib><creatorcontrib>Camera, Emanuela</creatorcontrib><creatorcontrib>Ludovici, Matteo</creatorcontrib><creatorcontrib>Picardo, Mauro</creatorcontrib><creatorcontrib>Müller, Udo</creatorcontrib><creatorcontrib>Leonhardt, Heinrich</creatorcontrib><creatorcontrib>Zouboulis, Christos C.</creatorcontrib><creatorcontrib>Schneider, Marlon R.</creatorcontrib><title>EGFR/ERBB receptors differentially modulate sebaceous lipogenesis</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>•EGFR, ERBB2 and ERBB3 tyrosine kinase receptors are expressed in human sebocytes.•Expression of EGFR/ERBBs is downregulated during sebaceous lipogenesis.•Downregulation of EGFR and ERBB3 using siRNAs increases lipogenesis.•EGFR/ERBB downregulation differentially modulates the expression of lipogenic markers.
The roles of the epidermal growth factor receptor (EGFR) in sebaceous glands remain poorly explored. We show that human sebocytes express EGFR and lower levels of ERBB2 and ERBB3, all receptors being downregulated after the induction of lipid synthesis. Nile red staining showed that siRNA-mediated downregulation of EGFR or ERBB3 increases lipid accumulation, whereas ERBB2 downregulation has no effect. Spectrometry confirmed induction of triglycerides after EGFR or ERBB3 downregulation and revealed induction of cholesteryl esters after downregulation of EGFR, ERBB2 or ERBB3. Thus, EGFR/ERBB receptors differentially modulate sebaceous lipogenesis, a key feature of sebaceous gland physiology and of several skin diseases.</description><subject>Biomarkers - metabolism</subject><subject>Cell Line</subject><subject>Cholesterol Esters - metabolism</subject><subject>Down-Regulation</subject><subject>Epidermal growth factor receptor</subject><subject>ERBB</subject><subject>Fatty Acids, Nonesterified - metabolism</subject><subject>Gene Expression Regulation, Enzymologic</subject><subject>Humans</subject><subject>Ligands</subject><subject>Linoleic Acid - metabolism</subject><subject>Lipogenesis</subject><subject>Receptor, Epidermal Growth Factor - antagonists & inhibitors</subject><subject>Receptor, Epidermal Growth Factor - genetics</subject><subject>Receptor, Epidermal Growth Factor - metabolism</subject><subject>Receptor, ErbB-2 - antagonists & inhibitors</subject><subject>Receptor, ErbB-2 - genetics</subject><subject>Receptor, ErbB-2 - metabolism</subject><subject>Receptor, ErbB-3 - antagonists & inhibitors</subject><subject>Receptor, ErbB-3 - genetics</subject><subject>Receptor, ErbB-3 - metabolism</subject><subject>RNA Interference</subject><subject>RNA, Small Interfering</subject><subject>Sebaceous gland</subject><subject>Sebaceous Glands - enzymology</subject><subject>Sebaceous Glands - metabolism</subject><subject>Sebaceous lipogenesis</subject><subject>Triglycerides - metabolism</subject><subject>Up-Regulation</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkUFvEzEQha0K1IbSnwDaI5fdju31rn1CTZW0SJWQCpwtrz2uHDnZYG-K8u_xKoErnEYjvXkz8z1CPlBoKNDudtN4HHLEqWFARQNtA8AvyILKnte87eQbsgCgbS16xa_Iu5w3UHpJ1SW5YkJK1fF-Qe5WD-vn29XzclkltLifxpQrF7zHhLspmBiP1XZ0h2gmrDIOxuJ4yFUM-_EFd5hDfk_eehMz3pzrNfmxXn2_f6yfvj58ub97qq1oe1VzlM44PtgeB9HhAL6nygjpOwGqF8ow1YPkwjnrh86gZ07S8sdgmPScAb8mn06--zT-PGCe9DZkizGa3XySpp2krPwkWJGKk9SmMeeEXu9T2Jp01BT0TE9v9JmenulpaHWhV-Y-nlcchi26v1N_cBXB40nwK0Q8_p-rXq-W7NscxZwEFQCsBVWsPp-ssDB7DZh0tgF3Fl0oQUzajeEf1_4GFm6Ycg</recordid><startdate>20150522</startdate><enddate>20150522</enddate><creator>Dahlhoff, Maik</creator><creator>Camera, Emanuela</creator><creator>Ludovici, Matteo</creator><creator>Picardo, Mauro</creator><creator>Müller, Udo</creator><creator>Leonhardt, Heinrich</creator><creator>Zouboulis, Christos C.</creator><creator>Schneider, Marlon R.</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20150522</creationdate><title>EGFR/ERBB receptors differentially modulate sebaceous lipogenesis</title><author>Dahlhoff, Maik ; Camera, Emanuela ; Ludovici, Matteo ; Picardo, Mauro ; Müller, Udo ; Leonhardt, Heinrich ; Zouboulis, Christos C. ; Schneider, Marlon R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5479-3e8dad3bc7eb56eb0f719a58f6509759a2970835ddcfb6aef2d81346ba28f3203</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Biomarkers - metabolism</topic><topic>Cell Line</topic><topic>Cholesterol Esters - metabolism</topic><topic>Down-Regulation</topic><topic>Epidermal growth factor receptor</topic><topic>ERBB</topic><topic>Fatty Acids, Nonesterified - metabolism</topic><topic>Gene Expression Regulation, Enzymologic</topic><topic>Humans</topic><topic>Ligands</topic><topic>Linoleic Acid - metabolism</topic><topic>Lipogenesis</topic><topic>Receptor, Epidermal Growth Factor - antagonists & inhibitors</topic><topic>Receptor, Epidermal Growth Factor - genetics</topic><topic>Receptor, Epidermal Growth Factor - metabolism</topic><topic>Receptor, ErbB-2 - antagonists & inhibitors</topic><topic>Receptor, ErbB-2 - genetics</topic><topic>Receptor, ErbB-2 - metabolism</topic><topic>Receptor, ErbB-3 - antagonists & inhibitors</topic><topic>Receptor, ErbB-3 - genetics</topic><topic>Receptor, ErbB-3 - metabolism</topic><topic>RNA Interference</topic><topic>RNA, Small Interfering</topic><topic>Sebaceous gland</topic><topic>Sebaceous Glands - enzymology</topic><topic>Sebaceous Glands - metabolism</topic><topic>Sebaceous lipogenesis</topic><topic>Triglycerides - metabolism</topic><topic>Up-Regulation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dahlhoff, Maik</creatorcontrib><creatorcontrib>Camera, Emanuela</creatorcontrib><creatorcontrib>Ludovici, Matteo</creatorcontrib><creatorcontrib>Picardo, Mauro</creatorcontrib><creatorcontrib>Müller, Udo</creatorcontrib><creatorcontrib>Leonhardt, Heinrich</creatorcontrib><creatorcontrib>Zouboulis, Christos C.</creatorcontrib><creatorcontrib>Schneider, Marlon R.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dahlhoff, Maik</au><au>Camera, Emanuela</au><au>Ludovici, Matteo</au><au>Picardo, Mauro</au><au>Müller, Udo</au><au>Leonhardt, Heinrich</au><au>Zouboulis, Christos C.</au><au>Schneider, Marlon R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>EGFR/ERBB receptors differentially modulate sebaceous lipogenesis</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>2015-05-22</date><risdate>2015</risdate><volume>589</volume><issue>12</issue><spage>1376</spage><epage>1382</epage><pages>1376-1382</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>•EGFR, ERBB2 and ERBB3 tyrosine kinase receptors are expressed in human sebocytes.•Expression of EGFR/ERBBs is downregulated during sebaceous lipogenesis.•Downregulation of EGFR and ERBB3 using siRNAs increases lipogenesis.•EGFR/ERBB downregulation differentially modulates the expression of lipogenic markers.
The roles of the epidermal growth factor receptor (EGFR) in sebaceous glands remain poorly explored. We show that human sebocytes express EGFR and lower levels of ERBB2 and ERBB3, all receptors being downregulated after the induction of lipid synthesis. Nile red staining showed that siRNA-mediated downregulation of EGFR or ERBB3 increases lipid accumulation, whereas ERBB2 downregulation has no effect. Spectrometry confirmed induction of triglycerides after EGFR or ERBB3 downregulation and revealed induction of cholesteryl esters after downregulation of EGFR, ERBB2 or ERBB3. Thus, EGFR/ERBB receptors differentially modulate sebaceous lipogenesis, a key feature of sebaceous gland physiology and of several skin diseases.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>25889637</pmid><doi>10.1016/j.febslet.2015.04.003</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| source | Wiley Free Content; MEDLINE; Wiley Online Library Journals Frontfile Complete; Elsevier ScienceDirect Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Biomarkers - metabolism Cell Line Cholesterol Esters - metabolism Down-Regulation Epidermal growth factor receptor ERBB Fatty Acids, Nonesterified - metabolism Gene Expression Regulation, Enzymologic Humans Ligands Linoleic Acid - metabolism Lipogenesis Receptor, Epidermal Growth Factor - antagonists & inhibitors Receptor, Epidermal Growth Factor - genetics Receptor, Epidermal Growth Factor - metabolism Receptor, ErbB-2 - antagonists & inhibitors Receptor, ErbB-2 - genetics Receptor, ErbB-2 - metabolism Receptor, ErbB-3 - antagonists & inhibitors Receptor, ErbB-3 - genetics Receptor, ErbB-3 - metabolism RNA Interference RNA, Small Interfering Sebaceous gland Sebaceous Glands - enzymology Sebaceous Glands - metabolism Sebaceous lipogenesis Triglycerides - metabolism Up-Regulation |
| title | EGFR/ERBB receptors differentially modulate sebaceous lipogenesis |
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