Separation of five flavonoids from tartary buckwheat (Fagopyrum tataricum (L.) Gaertn) grains via off-line two dimensional high-speed counter-current chromatography
An off-line two dimensional (2D) high-speed counter-current chromatography (HSCCC) strategy was successfully used for preparative separation of five flavonoids from tartary buckwheat (Fagopyrum tataricum (L.) Gaertn) grains with different solvent systems for the first time in this paper. n-Hexane-et...
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| Veröffentlicht in: | Food chemistry 2015-11, Vol.186, p.153-159 |
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| creator | Jiang, Shujing Liu, Qi Xie, Yixi Zeng, Hualiang Zhang, Li Jiang, Xinyu Chen, Xiaoqing |
| description | An off-line two dimensional (2D) high-speed counter-current chromatography (HSCCC) strategy was successfully used for preparative separation of five flavonoids from tartary buckwheat (Fagopyrum tataricum (L.) Gaertn) grains with different solvent systems for the first time in this paper. n-Hexane-ethyl acetate-methanol-water 3:5:3:5 (v/v) was selected as the first dimension solvent system to purify quercetin (4) and kaempferol (5). The second dimension solvent system, ethyl acetate-n-butanol-water 7:3:10 (v/v), was used to isolate quercetin 3-O-rutinoside-3'-O-β-glucopyranoside (1), rutin (2) and kaempferol 3-rutinoside (3). The purities of these compounds were all above 96.0% and their structures were identified through UV, MS and (1)H NMR. The results indicated that the off-line 2D HSCCC is an efficient technique to isolate flavonoids compounds from grains. |
| doi_str_mv | 10.1016/j.foodchem.2014.08.120 |
| format | Article |
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Gaertn) grains via off-line two dimensional high-speed counter-current chromatography</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Jiang, Shujing ; Liu, Qi ; Xie, Yixi ; Zeng, Hualiang ; Zhang, Li ; Jiang, Xinyu ; Chen, Xiaoqing</creator><creatorcontrib>Jiang, Shujing ; Liu, Qi ; Xie, Yixi ; Zeng, Hualiang ; Zhang, Li ; Jiang, Xinyu ; Chen, Xiaoqing</creatorcontrib><description>An off-line two dimensional (2D) high-speed counter-current chromatography (HSCCC) strategy was successfully used for preparative separation of five flavonoids from tartary buckwheat (Fagopyrum tataricum (L.) Gaertn) grains with different solvent systems for the first time in this paper. n-Hexane-ethyl acetate-methanol-water 3:5:3:5 (v/v) was selected as the first dimension solvent system to purify quercetin (4) and kaempferol (5). The second dimension solvent system, ethyl acetate-n-butanol-water 7:3:10 (v/v), was used to isolate quercetin 3-O-rutinoside-3'-O-β-glucopyranoside (1), rutin (2) and kaempferol 3-rutinoside (3). The purities of these compounds were all above 96.0% and their structures were identified through UV, MS and (1)H NMR. The results indicated that the off-line 2D HSCCC is an efficient technique to isolate flavonoids compounds from grains.</description><identifier>ISSN: 0308-8146</identifier><identifier>EISSN: 1873-7072</identifier><identifier>DOI: 10.1016/j.foodchem.2014.08.120</identifier><identifier>PMID: 25976805</identifier><language>eng</language><publisher>England</publisher><subject>Chromatography, High Pressure Liquid ; Countercurrent Distribution ; Fagopyrum - chemistry ; Flavonoids - analysis ; Glucosides - analysis ; Kaempferols - analysis ; Magnetic Resonance Spectroscopy ; Plant Extracts - analysis ; Quercetin - analogs & derivatives ; Quercetin - analysis ; Rutin - analysis</subject><ispartof>Food chemistry, 2015-11, Vol.186, p.153-159</ispartof><rights>Copyright © 2014 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c311t-61086d4058dbd392d1ea4d0a1b25f36e55161810074ecd6759b234ea3ed5beb83</citedby><cites>FETCH-LOGICAL-c311t-61086d4058dbd392d1ea4d0a1b25f36e55161810074ecd6759b234ea3ed5beb83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25976805$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jiang, Shujing</creatorcontrib><creatorcontrib>Liu, Qi</creatorcontrib><creatorcontrib>Xie, Yixi</creatorcontrib><creatorcontrib>Zeng, Hualiang</creatorcontrib><creatorcontrib>Zhang, Li</creatorcontrib><creatorcontrib>Jiang, Xinyu</creatorcontrib><creatorcontrib>Chen, Xiaoqing</creatorcontrib><title>Separation of five flavonoids from tartary buckwheat (Fagopyrum tataricum (L.) Gaertn) grains via off-line two dimensional high-speed counter-current chromatography</title><title>Food chemistry</title><addtitle>Food Chem</addtitle><description>An off-line two dimensional (2D) high-speed counter-current chromatography (HSCCC) strategy was successfully used for preparative separation of five flavonoids from tartary buckwheat (Fagopyrum tataricum (L.) Gaertn) grains with different solvent systems for the first time in this paper. n-Hexane-ethyl acetate-methanol-water 3:5:3:5 (v/v) was selected as the first dimension solvent system to purify quercetin (4) and kaempferol (5). The second dimension solvent system, ethyl acetate-n-butanol-water 7:3:10 (v/v), was used to isolate quercetin 3-O-rutinoside-3'-O-β-glucopyranoside (1), rutin (2) and kaempferol 3-rutinoside (3). The purities of these compounds were all above 96.0% and their structures were identified through UV, MS and (1)H NMR. The results indicated that the off-line 2D HSCCC is an efficient technique to isolate flavonoids compounds from grains.</description><subject>Chromatography, High Pressure Liquid</subject><subject>Countercurrent Distribution</subject><subject>Fagopyrum - chemistry</subject><subject>Flavonoids - analysis</subject><subject>Glucosides - analysis</subject><subject>Kaempferols - analysis</subject><subject>Magnetic Resonance Spectroscopy</subject><subject>Plant Extracts - analysis</subject><subject>Quercetin - analogs & derivatives</subject><subject>Quercetin - analysis</subject><subject>Rutin - analysis</subject><issn>0308-8146</issn><issn>1873-7072</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kcFq3DAQhkVpabZpXyHouDnYHVm2rD2W0KSFhR7anoUsjdfa2pYryRv2ffKg1ZKkMDAD88__w3yE3DAoGTDx-Vj23lsz4FRWwOoSZMkqeEM2TLa8aKGt3pINcJCFZLW4Ih9iPAJA1sr35Kpqdq2Q0GzI009cdNDJ-Zn6nvbuhLQf9cnP3tlI--AnmnTIdabdav48DqgT3d7rg1_OYb0s886ZPG335S190BjSfEsPQbs50pPT2bYvRjcjTY-eWjfhHHOaHungDkMRF0RLjV_nhKEwawg4J2qGHKyTzzbLcP5I3vV6jPjppV-T3_dff919K_Y_Hr7ffdkXhjOWCsFACltDI21n-a6yDHVtQbOuanousGmYYJIBtDUaK9pm11W8Rs3RNh12kl-T7bPvEvzfFWNSk4sGx1HP6NeomJCsEsCbi1Q8S03wMQbs1RLclL-kGKgLIXVUr4TUhZACqTKhfHjzkrF2E9r_Z69I-D_9cJLl</recordid><startdate>20151101</startdate><enddate>20151101</enddate><creator>Jiang, Shujing</creator><creator>Liu, Qi</creator><creator>Xie, Yixi</creator><creator>Zeng, Hualiang</creator><creator>Zhang, Li</creator><creator>Jiang, Xinyu</creator><creator>Chen, Xiaoqing</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20151101</creationdate><title>Separation of five flavonoids from tartary buckwheat (Fagopyrum tataricum (L.) Gaertn) grains via off-line two dimensional high-speed counter-current chromatography</title><author>Jiang, Shujing ; Liu, Qi ; Xie, Yixi ; Zeng, Hualiang ; Zhang, Li ; Jiang, Xinyu ; Chen, Xiaoqing</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c311t-61086d4058dbd392d1ea4d0a1b25f36e55161810074ecd6759b234ea3ed5beb83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Chromatography, High Pressure Liquid</topic><topic>Countercurrent Distribution</topic><topic>Fagopyrum - chemistry</topic><topic>Flavonoids - analysis</topic><topic>Glucosides - analysis</topic><topic>Kaempferols - analysis</topic><topic>Magnetic Resonance Spectroscopy</topic><topic>Plant Extracts - analysis</topic><topic>Quercetin - analogs & derivatives</topic><topic>Quercetin - analysis</topic><topic>Rutin - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jiang, Shujing</creatorcontrib><creatorcontrib>Liu, Qi</creatorcontrib><creatorcontrib>Xie, Yixi</creatorcontrib><creatorcontrib>Zeng, Hualiang</creatorcontrib><creatorcontrib>Zhang, Li</creatorcontrib><creatorcontrib>Jiang, Xinyu</creatorcontrib><creatorcontrib>Chen, Xiaoqing</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jiang, Shujing</au><au>Liu, Qi</au><au>Xie, Yixi</au><au>Zeng, Hualiang</au><au>Zhang, Li</au><au>Jiang, Xinyu</au><au>Chen, Xiaoqing</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Separation of five flavonoids from tartary buckwheat (Fagopyrum tataricum (L.) Gaertn) grains via off-line two dimensional high-speed counter-current chromatography</atitle><jtitle>Food chemistry</jtitle><addtitle>Food Chem</addtitle><date>2015-11-01</date><risdate>2015</risdate><volume>186</volume><spage>153</spage><epage>159</epage><pages>153-159</pages><issn>0308-8146</issn><eissn>1873-7072</eissn><abstract>An off-line two dimensional (2D) high-speed counter-current chromatography (HSCCC) strategy was successfully used for preparative separation of five flavonoids from tartary buckwheat (Fagopyrum tataricum (L.) Gaertn) grains with different solvent systems for the first time in this paper. n-Hexane-ethyl acetate-methanol-water 3:5:3:5 (v/v) was selected as the first dimension solvent system to purify quercetin (4) and kaempferol (5). The second dimension solvent system, ethyl acetate-n-butanol-water 7:3:10 (v/v), was used to isolate quercetin 3-O-rutinoside-3'-O-β-glucopyranoside (1), rutin (2) and kaempferol 3-rutinoside (3). The purities of these compounds were all above 96.0% and their structures were identified through UV, MS and (1)H NMR. The results indicated that the off-line 2D HSCCC is an efficient technique to isolate flavonoids compounds from grains.</abstract><cop>England</cop><pmid>25976805</pmid><doi>10.1016/j.foodchem.2014.08.120</doi><tpages>7</tpages></addata></record> |
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| identifier | ISSN: 0308-8146 |
| ispartof | Food chemistry, 2015-11, Vol.186, p.153-159 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Chromatography, High Pressure Liquid Countercurrent Distribution Fagopyrum - chemistry Flavonoids - analysis Glucosides - analysis Kaempferols - analysis Magnetic Resonance Spectroscopy Plant Extracts - analysis Quercetin - analogs & derivatives Quercetin - analysis Rutin - analysis |
| title | Separation of five flavonoids from tartary buckwheat (Fagopyrum tataricum (L.) Gaertn) grains via off-line two dimensional high-speed counter-current chromatography |
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