Nuclear magnetic resonance microscopy as a non-invasive tool to study the development of lepidopteran pupae

The application of NMR microscopy to the non-invasive study of internal structures in lepidopteran pupae is described and examples presented for an immature pupa of Pieris brassicae and a mature female pupa of Graphiphora augur. By using a chemical shift selective imaging procedure it was possible t...

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Veröffentlicht in:Journal of insect physiology 1995, Vol.41 (5), p.419-424
Hauptverfasser: Goodman, B.A., Gordon, S.C., Chudek, J.A., Hunter, G., Woodford, J.A.T.
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container_end_page 424
container_issue 5
container_start_page 419
container_title Journal of insect physiology
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creator Goodman, B.A.
Gordon, S.C.
Chudek, J.A.
Hunter, G.
Woodford, J.A.T.
description The application of NMR microscopy to the non-invasive study of internal structures in lepidopteran pupae is described and examples presented for an immature pupa of Pieris brassicae and a mature female pupa of Graphiphora augur. By using a chemical shift selective imaging procedure it was possible to determine separately the distributions of water and lipid. This technique makes it possible, in principle, to observe the relationship between the progress of metamorphosis and the utilization of lipid reserves and could thus represent a valuable new approach to the study of pupal developmental processes.
doi_str_mv 10.1016/0022-1910(94)00115-W
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ispartof Journal of insect physiology, 1995, Vol.41 (5), p.419-424
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subjects Chemical shift selective
Lepidoptera
NMR microimaging
Noctuidae
Pieridae
Pupal development
title Nuclear magnetic resonance microscopy as a non-invasive tool to study the development of lepidopteran pupae
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