Expression profiling of major heat shock protein genes during different seasons in cattle (Bos indicus) and buffalo (Bubalus bubalis) under tropical climatic condition
Heat shock proteins consist of highly conserved stress proteins, expressed in response to stress and play crucial roles in environmental stress tolerance and adaptation. The present study was conducted to identify major types of genes under the HSP70 family and other HSPs and to evaluate their expre...
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| Veröffentlicht in: | Journal of thermal biology 2015-07, Vol.51, p.55-64 |
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| creator | Kumar, Anil Ashraf, Syma Goud, T. Sridhar Grewal, Anita Singh, S.V. Yadav, B.R. Upadhyay, R.C. |
| description | Heat shock proteins consist of highly conserved stress proteins, expressed in response to stress and play crucial roles in environmental stress tolerance and adaptation. The present study was conducted to identify major types of genes under the HSP70 family and other HSPs and to evaluate their expression pattern in Sahiwal and Tharparkar breeds of zebu cattle (Bos indicus) and Murrah buffalo (Bubalus bubalis) with respect to different seasons. Quantitative real time polymerase chain reaction was performed to analyze the transcript variants of three HSP70 family genes (HSPA1A, HSPA1B, and HSPA8) and HSP10, HSP60, HSP90 and HSF1 in each breed. The major finding of this study was the higher abundance of all the studied HSP genes during summer and winter compared to spring season, but the magnitude of increase was higher during summer as compared to winter. HSPA1A and HSPA1B genes showed maximal induction (P |
| doi_str_mv | 10.1016/j.jtherbio.2015.03.006 |
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•Peripheral blood mononuclear cells resistance to heat stress was assessed in zebu breeds and Murrah.•Expression of HSPA8 gene was higher, but that of HSPA1A and HSPA2 was lower in Tharparkar than buffalo.•Expression of HSP90, HSP 60, HSP 10 and HSF1 was higher during summer season compare to spring and winter season.•Lymphocytes of zebu cattle breeds are more adapted to tropical climatic condition than buffalo.</description><identifier>ISSN: 0306-4565</identifier><identifier>EISSN: 1879-0992</identifier><identifier>DOI: 10.1016/j.jtherbio.2015.03.006</identifier><identifier>PMID: 25965018</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Acclimatization - genetics ; Animals ; Buffaloes ; Cattle ; Heat shock protein genes ; Heat-Shock Proteins - blood ; Heat-Shock Proteins - genetics ; Heat-Shock Proteins - metabolism ; Heat-Shock Response - genetics ; Murrah ; PBMC ; RNA, Messenger - metabolism ; Sahiwal ; Seasons ; Tharparkar ; Transcriptome ; Tropical Climate ; Zebu cattle</subject><ispartof>Journal of thermal biology, 2015-07, Vol.51, p.55-64</ispartof><rights>2015 Elsevier Ltd</rights><rights>Copyright © 2015 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c487t-58c5ae81ccbb6c903ed1f60a25a9258bd1f8b29dc54c5b1ed3ff4f636b4a65623</citedby><cites>FETCH-LOGICAL-c487t-58c5ae81ccbb6c903ed1f60a25a9258bd1f8b29dc54c5b1ed3ff4f636b4a65623</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jtherbio.2015.03.006$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25965018$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kumar, Anil</creatorcontrib><creatorcontrib>Ashraf, Syma</creatorcontrib><creatorcontrib>Goud, T. Sridhar</creatorcontrib><creatorcontrib>Grewal, Anita</creatorcontrib><creatorcontrib>Singh, S.V.</creatorcontrib><creatorcontrib>Yadav, B.R.</creatorcontrib><creatorcontrib>Upadhyay, R.C.</creatorcontrib><title>Expression profiling of major heat shock protein genes during different seasons in cattle (Bos indicus) and buffalo (Bubalus bubalis) under tropical climatic condition</title><title>Journal of thermal biology</title><addtitle>J Therm Biol</addtitle><description>Heat shock proteins consist of highly conserved stress proteins, expressed in response to stress and play crucial roles in environmental stress tolerance and adaptation. The present study was conducted to identify major types of genes under the HSP70 family and other HSPs and to evaluate their expression pattern in Sahiwal and Tharparkar breeds of zebu cattle (Bos indicus) and Murrah buffalo (Bubalus bubalis) with respect to different seasons. Quantitative real time polymerase chain reaction was performed to analyze the transcript variants of three HSP70 family genes (HSPA1A, HSPA1B, and HSPA8) and HSP10, HSP60, HSP90 and HSF1 in each breed. The major finding of this study was the higher abundance of all the studied HSP genes during summer and winter compared to spring season, but the magnitude of increase was higher during summer as compared to winter. HSPA1A and HSPA1B genes showed maximal induction (P<0.001) during summer and winter while HSP60 and HSP10 were found to be the second most abundantly expressed HSPs. The relative mRNA abundance of HSF1 significantly increased (P<0.001) in Murrah buffalo compared to Tharparkar and Sahiwal cattle during summer and winter. Expression pattern of heat shock protein genes indicated that amongst the breeds, the expression was higher in Murrah buffalo compared to Sahiwal and Tharparkar cattle, thereby indicating the more adaptive capacity of later during periods of stress. Hence, this study suggests that heat shock protein genes may be conveniently used as biomarkers for assessing stress response in cattle and buffalo and the expression is species and breed-specific. Furthermore, the variation in expression is associated with heat tolerance and adaptation to different climatic conditions.
•Peripheral blood mononuclear cells resistance to heat stress was assessed in zebu breeds and Murrah.•Expression of HSPA8 gene was higher, but that of HSPA1A and HSPA2 was lower in Tharparkar than buffalo.•Expression of HSP90, HSP 60, HSP 10 and HSF1 was higher during summer season compare to spring and winter season.•Lymphocytes of zebu cattle breeds are more adapted to tropical climatic condition than buffalo.</description><subject>Acclimatization - genetics</subject><subject>Animals</subject><subject>Buffaloes</subject><subject>Cattle</subject><subject>Heat shock protein genes</subject><subject>Heat-Shock Proteins - blood</subject><subject>Heat-Shock Proteins - genetics</subject><subject>Heat-Shock Proteins - metabolism</subject><subject>Heat-Shock Response - genetics</subject><subject>Murrah</subject><subject>PBMC</subject><subject>RNA, Messenger - metabolism</subject><subject>Sahiwal</subject><subject>Seasons</subject><subject>Tharparkar</subject><subject>Transcriptome</subject><subject>Tropical Climate</subject><subject>Zebu cattle</subject><issn>0306-4565</issn><issn>1879-0992</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc9u1DAQxi0EokvhFSofyyHBTmJvcgOq8keqxAXOlj0edx2y9mI7CJ6I18TRtlw5jcbf7_PM6CPkirOWMy7fzO1cDpiMj23HuGhZ3zImn5AdH_dTw6ape0p2rGeyGYQUF-RFzjOrYC_Yc3LRiUkKxscd-XP765QwZx8DPaXo_OLDPY2OHvUcEz2gLjQfInzf1II-0HsMmKld0wZa7xwmDBVCnWPItBKgS1mQXr-PW2s9rPk11cFSszqnl1iV1ehlzfWhVl_VNVhMtKR48qAXCos_6uKBQqz-Upd7SZ5Va8ZXD_WSfPtw-_XmU3P35ePnm3d3DQzjvjRiBKFx5ADGSJhYj5Y7yXQn9NSJ0dRuNN1kQQwgDEfbOzc42UszaClk11-S6_O_9dwfK-aijj4DLosOGNesuBzZJMTY7ysqzyikmHNCp06prp1-K87UFpKa1WNIagtJsV7VkKrx6mHGao5o_9keU6nA2zOA9dKfHpPK4DEAWp8QirLR_2_GX8UrqvM</recordid><startdate>201507</startdate><enddate>201507</enddate><creator>Kumar, Anil</creator><creator>Ashraf, Syma</creator><creator>Goud, T. Sridhar</creator><creator>Grewal, Anita</creator><creator>Singh, S.V.</creator><creator>Yadav, B.R.</creator><creator>Upadhyay, R.C.</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201507</creationdate><title>Expression profiling of major heat shock protein genes during different seasons in cattle (Bos indicus) and buffalo (Bubalus bubalis) under tropical climatic condition</title><author>Kumar, Anil ; Ashraf, Syma ; Goud, T. Sridhar ; Grewal, Anita ; Singh, S.V. ; Yadav, B.R. ; Upadhyay, R.C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c487t-58c5ae81ccbb6c903ed1f60a25a9258bd1f8b29dc54c5b1ed3ff4f636b4a65623</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Acclimatization - genetics</topic><topic>Animals</topic><topic>Buffaloes</topic><topic>Cattle</topic><topic>Heat shock protein genes</topic><topic>Heat-Shock Proteins - blood</topic><topic>Heat-Shock Proteins - genetics</topic><topic>Heat-Shock Proteins - metabolism</topic><topic>Heat-Shock Response - genetics</topic><topic>Murrah</topic><topic>PBMC</topic><topic>RNA, Messenger - metabolism</topic><topic>Sahiwal</topic><topic>Seasons</topic><topic>Tharparkar</topic><topic>Transcriptome</topic><topic>Tropical Climate</topic><topic>Zebu cattle</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kumar, Anil</creatorcontrib><creatorcontrib>Ashraf, Syma</creatorcontrib><creatorcontrib>Goud, T. Sridhar</creatorcontrib><creatorcontrib>Grewal, Anita</creatorcontrib><creatorcontrib>Singh, S.V.</creatorcontrib><creatorcontrib>Yadav, B.R.</creatorcontrib><creatorcontrib>Upadhyay, R.C.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of thermal biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kumar, Anil</au><au>Ashraf, Syma</au><au>Goud, T. Sridhar</au><au>Grewal, Anita</au><au>Singh, S.V.</au><au>Yadav, B.R.</au><au>Upadhyay, R.C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression profiling of major heat shock protein genes during different seasons in cattle (Bos indicus) and buffalo (Bubalus bubalis) under tropical climatic condition</atitle><jtitle>Journal of thermal biology</jtitle><addtitle>J Therm Biol</addtitle><date>2015-07</date><risdate>2015</risdate><volume>51</volume><spage>55</spage><epage>64</epage><pages>55-64</pages><issn>0306-4565</issn><eissn>1879-0992</eissn><abstract>Heat shock proteins consist of highly conserved stress proteins, expressed in response to stress and play crucial roles in environmental stress tolerance and adaptation. The present study was conducted to identify major types of genes under the HSP70 family and other HSPs and to evaluate their expression pattern in Sahiwal and Tharparkar breeds of zebu cattle (Bos indicus) and Murrah buffalo (Bubalus bubalis) with respect to different seasons. Quantitative real time polymerase chain reaction was performed to analyze the transcript variants of three HSP70 family genes (HSPA1A, HSPA1B, and HSPA8) and HSP10, HSP60, HSP90 and HSF1 in each breed. The major finding of this study was the higher abundance of all the studied HSP genes during summer and winter compared to spring season, but the magnitude of increase was higher during summer as compared to winter. HSPA1A and HSPA1B genes showed maximal induction (P<0.001) during summer and winter while HSP60 and HSP10 were found to be the second most abundantly expressed HSPs. The relative mRNA abundance of HSF1 significantly increased (P<0.001) in Murrah buffalo compared to Tharparkar and Sahiwal cattle during summer and winter. Expression pattern of heat shock protein genes indicated that amongst the breeds, the expression was higher in Murrah buffalo compared to Sahiwal and Tharparkar cattle, thereby indicating the more adaptive capacity of later during periods of stress. Hence, this study suggests that heat shock protein genes may be conveniently used as biomarkers for assessing stress response in cattle and buffalo and the expression is species and breed-specific. Furthermore, the variation in expression is associated with heat tolerance and adaptation to different climatic conditions.
•Peripheral blood mononuclear cells resistance to heat stress was assessed in zebu breeds and Murrah.•Expression of HSPA8 gene was higher, but that of HSPA1A and HSPA2 was lower in Tharparkar than buffalo.•Expression of HSP90, HSP 60, HSP 10 and HSF1 was higher during summer season compare to spring and winter season.•Lymphocytes of zebu cattle breeds are more adapted to tropical climatic condition than buffalo.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>25965018</pmid><doi>10.1016/j.jtherbio.2015.03.006</doi><tpages>10</tpages></addata></record> |
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| ispartof | Journal of thermal biology, 2015-07, Vol.51, p.55-64 |
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| subjects | Acclimatization - genetics Animals Buffaloes Cattle Heat shock protein genes Heat-Shock Proteins - blood Heat-Shock Proteins - genetics Heat-Shock Proteins - metabolism Heat-Shock Response - genetics Murrah PBMC RNA, Messenger - metabolism Sahiwal Seasons Tharparkar Transcriptome Tropical Climate Zebu cattle |
| title | Expression profiling of major heat shock protein genes during different seasons in cattle (Bos indicus) and buffalo (Bubalus bubalis) under tropical climatic condition |
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